SacI-HF from New England Biolabs

Product Specs
ItemSacI-HF
CompanyNew England Biolabs
Price $273.00 Inquire
Catalog NumberR3156L
Quantity10000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Workflow for Gene Overexpression and Phenotypic Characterisation in Taraxacum kok-saghyzMethods and ProtocolsJanuary 24, 2026Loredana Lopez, Michele Antonio Savoia, Loretta Daddiego, Paolo Facella, Elio Fantini, Linda Bianco, Simone Maci, Francesco PanaraGermany; Cat. no.: P1379).XbaI and SacI (New England Biolabs, Ipswich, MA, USA; Cat. no.: R0145L and R3156L). 2.2. Equipment Standard laboratory equipment: analytical balance; autoclave; electroporator (MicroPulser Electroporator

(2) Multiple oestradiol functions inhibit ferroptosis and acute kidney injuryNatureSeptember 25, 2025Wulf Tonnus, Francesca Maremonti, Shubhangi Gavali, Marlena Nastassja Schlecht, Florian Gembardt, Alexia Belavgeni, Nadja Leinung, Karolin Flade, Natalie Bethe, Sofia Traikov, et al.(Macherey-Nagel, 740410.50). To verify plasmid integrity, the isolated plasmids were digested with SacI-HF (NEB, R3156L) and resolved on 1% agarose gel. Transfection was performed using the Neon NxT electroporation system

(3) Split-and-pool synthesis to generate scalable combinatorial oligonucleotide libraries on magnetic nanoparticlesSTAR ProtocolsJune 20, 2025John V.L. Nguyen, Lidija Malic, Christina Nassif, Dillon Da Fonte, Maryam Tabrizian,New England Biolabs,Cat# M0491L Q5 reaction buffer pack,New England Biolabs,Cat# B9027S SacI-HF,New England Biolabs,Cat# R3156L "Tris (base), ultra pure",BioShop Canada,Cat# TRS001.500 ,, Critical commercial assays,Critical commercial

(4) Redox regulation of G protein oligomerization and signaling by the glutaredoxin WG1 controls grain size in riceThe EMBO JournalMay 19, 2025Lijie Liu, Jianqin Hao, Ke Huang, Penggen Duan, Baolan Zhang, Zhihai Chi, Xiaohong Yao, Yunhai Li,New England Biolabs,R3142L Bgl II,New England Biolabs,R0144L EcoR I,New England Biolabs,R3101S Sac I,New England Biolabs,R3156L Pst I,New England Biolabs,R0140S Nco I,New England Biolabs,R3193L Xba I,New England Biolabs,R0145L Sal

(5) p53 regulates DREAM complex-mediated repression in a p21-independent mannerThe EMBO JournalMarch 4, 2025Ritu Agrawal, Sagar Sengupta,New England BioLabs,Cat# R0104L BamHI HF,New England BioLabs,Cat# R0136L SacI,New England BioLabs,Cat# R3156L XhoI,New England BioLabs,Cat# R0146L GST,This study,N/A GST p53 (1-393),This study,N/A GST p53 (1-75),This

(6) Raiding nature's genetic toolbox for UV-C resistance by functional metagenomicsSci RepJanuary 2, 2025Garrett A. Roberts Kingman, Justin L. Kipness, Lynn J. Rothschildrestriction digest-based cloning methods were used, DNA was digested with BamHI-HF (NEB #R3136L) and SacI-HF (NEB #R3156L) in 1x rCutSmart buffer (NEB #B6004S), products gel-purified (Zymo Research Zymoclean Gel DNA Recovery Kit

(7) STK19 positions TFIIH for cell-free transcription-coupled DNA repairCellDecember 12, 2024Tycho E.T. Mevissen, Maximilian Kümmecke, Ernst W. Schmid, Lucas Farnung, Johannes C. Walterproteins","Chemicals, peptides, and recombinant proteins" BamHI-HF,NEB,Cat# R3136M SacI-HF,NEB,Cat# R3156L BbsI-HF,NEB,Cat# R3539L PmlI,NFR,Cat# R0532S EcoRI-HF,NEB,Cat# R3101S KpnI-HF,NEB,Cat# R3142S XhoI,NEB,Cat#

(8) Targeted Replacement of HSF1 Phosphorylation Sites at S303/S307 with Alanine Residues in Mice Increases Cell Proliferation and Drug ResistanceMethods in Molecular BiologyJuly 11, 2024Xiongjie Jin, Demetrius Moskophidis, Nahid F. MivechiEndonuclease (New England Biolabs, cat no. R0197L) SacI Restriction Endonuclease (New England Biolabs, cat no. R3156L) BglII Restriction Endonuclease (New England Biolabs, cat no. R0144L) NotI Restriction Endonuclease

(9) Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-CasSTAR ProtocolsMarch 15, 2024Adrian Pascal Nievergelt, Dennis Ray Diener, Aliona Bogdanova, Thomas Brown, Gaia PiginoPlus,Biotium,31077-T GelGreen,Biotium,41005 Betaine,Merck,61962 NEBuilder HiFi assembly master mix,NEB,E2621L BspQI,NEB,R0712L BtsI-v2,NEB,R0667L SacI-HF,NEB,R3156L EcoRI-HF,NEB,R3101L NheI-HF,NEB,R3131S Hutner’s trace elements,Chlamydomonas Resource Center,N/A IGEPAL

(10) Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-CasCell Reports MethodsAugust 22, 2023Adrian Pascal Nievergelt, Dennis Ray Diener, Aliona Bogdanova, Thomas Brown, Gaia PiginoPlus,Biotinum,31077-T GelGreen,Biotinum,41005 Betaine,Merck,61962 NEBuilder HiFi assembly MM,NEB,E2621L BspQI,NEB,R0712L SacI-HF,NEB,R3156L EcoRI-HF,NEB,R3101L NheI-HF,NEB,R3131S Hutner’s Trace Elements,Chlamydomonas Resource Center,N/A IGEPAL

(11) Systematic characterization of regulatory variants of blood pressure genesCell GenomicsJuly 12, 2023Winona Oliveros, Kate Delfosse, Daniella F. Lato, Katerina Kiriakopulos, Milad Mokhtaridoost, Abdelrahman Said, Brandon J. McMurray, Jared W.L. Browning, Kaia Mattioli, Guoliang Meng, et al.recombinant,Worthington Biochemical,Cat#LS006355 T4 Polynucleotide Kinase,NEB,Cat#M0201L SacI-HF,NEB,Cat#R3156L Lipofectamine 3000,Invitrogen,Cat#L3000008 Lipofectamine Stem Transfection Reagent,Invitrogen,Cat#STEM00015 "Recombinant Human EGF Protein

(12) A two-step adaptive walk rewires nutrient transport in a challenging edaphic environmentScience AdvancesMay 20, 2022Emmanuel Tergemina et al.389) by using the restriction enzymes Dde I (NEB, catalog no. R0175S) and Sac I–HF (NEB, catalog no. R3156L), respectively. We used dCAPS finder 2.0 (http://helix.wustl.edu/dcaps/dcaps.html) to generate the PCR

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SacI-HF

SacI-HF from New England Biolabs

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New England Biolabs

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SacI-HF from New England Biolabs

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