SspI-HF from New England Biolabs

Product Specs
ItemSspI-HF
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR3132S
Quantity1000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

AAT⇓ATT
TTA⇑TAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Homology-mediated transformation of frog-killing fungus Batrachochytrium dendrobatidis illuminates chytrid development and pathogenesisProceedings of the National Academy of SciencesNovember 4, 2025Stephanie M. Brody, Erik Kalinka, Sarah M. Prostak, Tamilie Carvalho, Jarrett Man, Timothy Y. James, Lillian K. Fritz-Laylinincubated 100 µg of plasmid DNA with 100 units of restriction enzyme SspI-HF (New England Biolabs, Cat. No. R3132S) at 37 °C for 18 h, then heat inactivated by incubation at 65 °C for 20 min. To precipitate the DNA,

(2) Protocol for generation of transmitochondrial cybrids under pyruvate/uridine-supplemented conditions using a microfluidic deviceSTAR ProtocJuly 17, 2025Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda, Yui Harada, Yoshikazu YonemitsuLtd.",KOD-211 Proteinase K solution,"Kanto Chemical Co., Inc.",34060–96 SspI-HF (SspI),New England Biolabs,R3132S 1 M Tris-HCl (pH 8.0),"Nippon Gene Co., Ltd.",312–90061 Tween 20,"Nacalai Tesque, Inc.",35624–02 Tris-Borate-EDTA

(3) Identification of regions required for allelic specificity at the cell wall remodeling allorecognition checkpoint in Neurospora crassaGENETICSApril 4, 2025Adriana M Rico-Ramirez, N Louise Glassbackground were subjected to transformation with PacI (R0547S, NEB), NdeI (R0111S, NEB), or SspI-HF (R3132S, NEB) linearized plasmids. Following established protocols (Margolin et al. 1997), transformation was

(4) Efficient Nonviral Integration of Large Transgenes into Human T Cells Using Cas9-CLIPTMolecular Therapy Methods & Clinical DevelopmentMarch 13, 2025Anna Tommasi, Dan Cappabianca, Madison Bugel, Kirstan Gimse, Karl Lund-Peterson, Hum Shrestha, Denis Arutyunov, James A. Williams, Seshidhar Reddy Police, Venkata Indurthi, et al.a dsDNA HDR template, a restriction digest of the Nanoplasmid construct using SspI-HF (catalog no. R3132S, New England Biolabs [NEB], Ipswich, MA) was performed. Four restriction digest batch reactions in 1.5-mL

(5) TGF-β and RAS jointly unmask primed enhancers to drive metastasisCellOctober 31, 2024Jun Ho Lee, Francisco J. Sánchez-Rivera, Lan He, Harihar Basnet, Fei Xavier Chen, Elena Spina, Liangji Li, Carles Torner, Jason E. Chan, Dig Vijay Kumar Yarlagadda, et al.(BsrGI (NEB, Cat #: R3575S), EcoRI (NEB, Cat #: R3101S), HindIII (NEB, Cat #: R3104S), SspI (NEB, Cat #: R3132S), XbaI (NEB, Cat #: R0145S)) in NEB buffer 2 with 1 mM spermidine (Sigma Aldrich, Cat #: 05292-1ML-F)

(6) Intracellular delivery of oncolytic viruses with engineered Salmonella causes viral replication and cell deathiScienceJune 21, 2024Shradha Khanduja, Shoshana M.K. Bloom, Vishnu Raman, Chinmay P. Deshpande, Christopher L. Hall, Neil S. ForbesBP918-1 Chloramphenicol,Fisher Scientific,Cat# 227921000 SSPI-HF restriction enzyme,New England Biolabs,Cat# R3132S "Laemmli SDS sample buffer, reducing (6X)",Fisher Scientific,Cat# J61337.AC Crystal Violet,Acros organics,229641000 Hoechst

(7) Avoidance of thiazoline compound depends on multiple sensory pathways mediated by TrpA1 and ORs in DrosophilaFrontiers in Molecular NeuroscienceDecember 22, 2023Shoma Sato, Aliyu Mudassir Magaji, Makoto Tominaga, Takaaki SokabeShiga). The presence of dTrpA1-C or dTrpA1-D in the constructs was confirmed by digestion with SspI-HF (#R3132S, NEB, MA), resulted in the following fragments: TrpA1-C/pAc5.1-V5-His A: 4063, 2751, 992, 637, 321,

(8) Glucocorticoids unmask silent non-coding genetic risk variants for common diseasesNucleic Acids ResearchNovember 11, 2022Thanh Thanh L Nguyen, Huanyao Gao, Duan Liu, Trudy Janice Philips, Zhenqing Ye, Jeong-Heon Lee, Geng-xian Shi, Kaleigh Copenhaver, Lingxin Zhang, Lixuan Wei, et al.The plasmid was digested with restriction enzymes BamHI-HF® (Cat#: R3136S, NEB) and SspI-HF® (Cat#: R3132S, NEB). We used Gibson Assembly cloning approach, the conditions of which were similar to those described

(9) A moonlighting function of a chitin polysaccharide monooxygenase, CWR-1, in Neurospora crassa allorecognitioneLifeAugust 30, 2022Tyler C Detomasi, Adriana M Rico-RamÃrez, Richard I Sayler, A Pedro GonÃ§alves, Michael A Marletta, N Louise GlassMiniprep Kit (QIAGEN, Redwood City, CA; Cat# 27104) and linearized with NdeI (NEB, R0111S), SspI-HF (R3132S), or PciI (NEB, R0655S) to transform N. crassa. Strain and culture conditions The standard protocols

(10) Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexesMethods in EnzymologyOctober 14, 2019Nicholas J. Porter, David W. Christianson10X stock (New England BioLabs #B7240S) 14. SspI-HF restriction endonuclease (New England Biolabs #R3132S) 15. QIAquick PCR Purification Kit (QIAGEN #28104) 16. NEBuffer™ 2.1 (New England BioLabs #B7202S) 17. Deoxycytidine

(11) R-ChIP Using Inactive RNase H Reveals Dynamic Coupling of R-loops with Transcriptional Pausing at Gene PromotersMolecular cellNovember 16, 2017Chen L, Chen JY, Zhang X, Gu Y, Xiao R, Shao C, Tang P, Qian H, Luo D, Li H, et al.Stain,Thermo Fisher,S-11494 HindIII-HF,NEB,R3104S BsrGI-HF,NEB,R3575S XbaI,NEB,R0145S SspI-HF,NEB,R3132S RNase H,NEB,M0297S Critical Commercial Assays,, PureLink PCR Micro Kit,Thermo Fisher,K310050 PureLink Quick Gel Extraction Kit

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SspI-HF

SspI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (11)

Reviews

SspI-HF from New England Biolabs

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