SphI from New England Biolabs

Product Specs
ItemSphI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0182S
Concentration5 u/µL
Quantity500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCATG/C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Long-read RNA sequencing of transposable elements from single cells using CELLO-seqNature ProtocolsJuly 16, 2025Sophie A. Marlow, Lauryn A. Deaville, Rebecca V. Berrensreaction, ensuring increased length and cDNA output.-dNTP Set, 100mM Solutions (Thermo Fisher Scientific: R0182)-HiFi Taq DNA Ligase (New England Biolabs: M0647S)CRITICAL: This ligase is required as it can be used

(2) A nucleosome switch primes hepatitis B virus infectionCellApril 17, 2025Nicholas A. Prescott, Tracy Biaco, Andrés Mansisidor, Yaron Bram, Justin Rendleman, Sarah C. Faulkner, Abigail A. Lemmon, Christine Lim, Rachel Tiersky, Eralda Salataj, et al.S2742 RNase A,Thermo Scientific,EN0531 S. cerevisiae Nap1,"Nguyen, et al. (2022)",N/A SphI,NEB,Cat# R0182S T4 Ligase,NEB,Cat# M0202M T5 Exonuclease,NEB,Cat# M0663S TransfeX Transfection Reagent,ATCC,Cat#

(3) Tat-fimbriae (“tafi”): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathwayiScienceFebruary 21, 2025Anna V. Galeva, Dahe Zhao, Alexey S. Syutkin, Marina Yu Topilina, Sergei Yu Shchyogolev, Elena Yu Pavlova, Olga M. Selivanova, Igor I. Kireev, Alexey K. Surin, Gennady L. Burygin, et al.proteins" ,, BamHI-HF®,NEB,R3136S EcoRI-HF®,NEB,R3101S KpnI-HF®,NEB,R3142S NdeI,NEB,R0111S PstI,NEB,R0140S Q5® High-Fidelity DNA Polymerase,NEB,M0491S SphI,NEB,R0182S DNase I,Thermo scientific,EN0521 BCIP/NBT Color Development Substrate,Promega,S3771 IPTG,Thermo scientific,R0392 L-Tryptophan,Merck,T0254 Bacto™

(4) Evaluation of Cas13d as a tool for genetic interaction mappingNat CommunFebruary 14, 2025Ghanem El Kassem, Jasmine Hillmer, Michael Boettchercut position by Gibson Assembly. The resulting plasmid was then digested with SphI and PaqCI (NEB, R0182S, R0745S) and purified as before. A gene fragment without the spCas9 tracrRNA was synthesized by Genscript

(5) A structural peculiarity of Antarctic fish IgM drives the generation of an engineered mAb by CRISPR/Cas9Frontiers in Bioengineering and BiotechnologyJuly 25, 2024Alessia Ametrano, Bruno Miranda, Rosalba Moretta, Principia Dardano, Luca De Stefano, Umberto Oreste, Maria Rosaria CosciaEndoFree Plasmid Maxi Kit (QIAGEN) and double-digested with HindIII (NEB, #R0104S) and SphI (NEB, #R0182S) at 37°C for 1 h (Ametrano and Coscia, 2022). 2.5 Hybridoma transfection with CRISPR/Cas9 and HDR donor

(6) Protocol to reveal the binding partner of secreted housekeeping enzymes in Listeria monocytogenes via in silico prediction to in vivo validationSTAR ProtocolsMarch 15, 2024Dongqi Liu, Harrison D.B. Helmick, Jozef L. Kokini, Arun K. BhuniaPlatinum Hot Start PCR master mix (2X),Thermo Fisher Scientific,Cat# 130000 T4 DNA ligase,NEB,Cat# M0202S BamHI,NEB,Cat# R0136 SphI,NEB,Cat# R0182 DNA clean & concentrator-5,Zymo,Cat# D4013 ZymoPURE plasmid miniprep,Zymo,Cat# D4208T NEB-5-alpha

(7) Gene duplication and deletion caused by over-replication at a fork barrierNature CommunicationsNovember 25, 2023Judith Oehler, Carl A. Morrow, Matthew C. Whitbyµl 10x ThermoPol buffer (New England Biolabs, M0267X), 0.6 µl 10 mM dNTPs (ThermoFisher Scientific, R0182); 0.6 µl 10 µM oMW1985, 0.6 µl 10 µM oMW1620/1628, and 0.15 µl of Taq DNA Polymerase (New England Biolabs,

(8) How Pol α-primase is targeted to replisomes to prime eukaryotic DNA replicationMolecular CellAugust 17, 2023Morgan L. Jones, Valentina Aria, Yasemin Baris, Joseph T.P. YeelesAM2616 Phusion® High-Fidelity DNA Polymerase,New England Biolabs,Cat# E0553 SphI,New England Biolabs,Cat# R0182 SapI,New England Biolabs,Cat# R0569 FspI,New England Biolabs,Cat# R0135 BsaHI,New England Biolabs,Cat#

(9) Biological features of TcM: A new Trypanosoma cruzi isolate from Argentina classified into TcV lineage✰Current Research in Microbial SciencesAugust 1, 2022Santiago José Martinez, Gonzalo Nicolás Nardella, Matías Exequiel Rodríguez, Cynthia Vanesa Rivero, Fernán Agüero, Patricia Silvia Romanoamplification products (832 bp) were simultaneously digested with 1 U of HpaI (NEB R0105) and 1 U of SphI (NEB R0182) for 1 h at 37 °C. Restriction fragments were then resolved in 2% TBE-agarose gel for 1 h at 5.33 V/cm.

(10) A Zika virus mutation enhances transmission potential and confers escape from protective dengue virus immunityCell ReportsApril 12, 2022Jose Angel Regla-Nava et al.Kit,Qiagen,Cat. #73404 AvrII,(New England BioLabs),Cat. #R0174S SphI,(New England BioLabs),Cat. #R0182S NaeI,(New England BioLabs),Cat. #R0190S ClaI,(New England BioLabs),Cat. #R0197S mMESSAGE mMACHINE™ T7 transcription kit

(11) Modelling Chlamydia and HPV co-infection in patient-derived ectocervix organoids reveals distinct cellular reprogrammingNature communicationsFebruary 24, 2022Koster S, Gurumurthy RK, Kumar N, Prakash PG, Dhanraj J, Bayer S, Berger H, Kurian SM, Drabkina M, Mollenkopf HJ, et al.µL forward primer (10 µM), 0.5 µl reverse primer (10 µM), 0.5 µl dNTPs (10 mM) (Thermo Scientific, #R0182), 2.5 µL 10x NEB buffer (BioLabs, #B9004S), 1 µL NEB Taq polymerase (5000 U/mL) (BioLabs, #M0267S),

(12) Mesmerize is a dynamically adaptable user-friendly analysis platform for 2D and 3D calcium imaging dataNature CommunicationsDecember 1, 2021Kushal Kolar, Daniel Dondorp, Jordi Cornelis Zwiggelaar, JÃ¸rgen HÃ¸yer, Marios ChatzigeorgiouPromega). Using purified gDNA at 150 ng/μl, the primers shown in Supplementary Table 5 dNTPs (Thermofisher, R0182) and the Q5 High-Fidelity DNA Polymerase (M0491L, NEB) we performed PCR reactions. The amplified PCR

(13) Dual RNA 3’-end processing of H2A.X messenger RNA maintains DNA damage repair throughout the cell cycleNature CommunicationsDecember 1, 2021Esther Griesbach, Margarita Schlackow, William F. Marzluff, Nick J. Proudfootpoly(A) site was excised using MluI and SphI restriction enzymes (New England Biolabs (NEB, #R0198S and #R0182S). The linearised plasmid was gel-purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research, #D4007)

(14) Analysis of Drosophila Atg8 proteins reveals multiple lipidation-independent rolesAutophagySeptember 2, 2021András Jipa, Viktor Vedelek, Zsolt Merényi, Adél Ürmösi, Szabolcs Takáts, Attila L. Kovács, Gábor V. Horváth, Rita Sinka, Gábor Juhász3xHA-Atg8b-Atg8aUTR intermediate construct. pGen-3xHA-Atg8b-UTR plasmid was digested with SphI (NEB, R0182S)-EcoRI (NEB, R3101) enzymes and ligated with SphI (NEB, R0182S)-EcoRI (R0101S) digested Atg8a promoter

(15) Implication of ZNF217 in Accelerating Tumor Development and Therapeutically Targeting ZNF217-Induced PI3K–AKT Signaling for the Treatment of Metastatic OsteosarcomaMolecular Cancer TherapeuticsDecember 1, 2020Branden A. Smeester et al.generated via the LR cloning system (#11791020, Thermo Fisher) for #3 prior to linearization with SphI (#R0182, New England Biolabs) and pronuclear injection described in the following section. Pronuclear injection Transgenic

(16) Detection of Individual RNA in Fixed Cells and Tissues by Chromogenic ISHBIO-PROTOCOLFebruary 5, 2020Meng Jiang, Chen Lin, Rongqin Kecontaining PNK buffer A), store at -20 °C 21. dNTP Set, 100 mM Solutions (Thermo Scientific, catalog number: R0182), store at -20 °C 22. SplintR Ligase, 25 U/µl (NEB, catalog number: M0375L), store at -20 °C 23. phi29 DNA Polymerase

(17) Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease ModelsJournal of Visualized ExperimentsApril 16, 2019Lidia Tagliafierro, Ekaterina Ilich, Malik Moncalvo, Jeffrey Gu, Ahila Sriskanda, Carole Grenier, Susan K. Murphy, Ornit Chiba-Falek, Boris KantorR0195S KpnI New England Biolabs R0142S PacI New England Biolabs R0547S SphI New England Biolabs R0182S Materials Company Catalog Number Comments

(18) DNA content analysis allows discrimination between Trypanosoma cruzi and Trypanosoma rangeliPLOS ONEDecember 19, 2017Lucila Langoni Naves, Marcos Vinícius da Silva, Emanuella Francisco Fajardo, Raíssa Bernardes da Silva, Fernanda Bernadelli De Vito, Virmondes Rodrigues, Eliane Lages-Silva, Luis Eduardo Ramírez, André Luiz Pedrosadigested with 1U of the enzyme HpaI (NEB R105) at 55˚C for 1 h and with 1U of the enzyme SphI (NEB R0182) at 37˚C for 1 h (TcSC5D fragment) or with one unit of XhoI (NEB R0146S) endonuclease (TcMK fragment). The

(19) Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesiseLifeSeptember 13, 2016Walentek P, Quigley IK, Sun DI, Sajjan UK, Kintner C, Harland RM.and subcloned into the pCS107 expression vector, which was digested with Sph1 (New England Biolabs, #R0182S) and re-ligated to remove the miR binding site, as previously described (Song et al., 2014). DNAs were

(20) miR-34/449 miRNAs are required for motile ciliogenesis by repressing cp110NatureJune 5, 2014Song R, Walentek P, Sponer N, Klimke A, Lee JS, Dixon G, Harland R, Wan Y, Lishko P, Lize M, et al.Cp110Δ3′UTR was generated from the same clone, which was digested with Sph1 (New England Biolabs, #R0182S) and re-ligated to remove most of the 3′UTR. Analysis of morphant tadpole brains for signs of hydrocephalus

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SphI

SphI from New England Biolabs

Sequence

New England Biolabs

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SphI from New England Biolabs

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