SnaBI from New England Biolabs

Product Specs
ItemSnaBI
CompanyNew England Biolabs
Price $67.00 Inquire
Catalog NumberR0130S
Quantity500 units
Concentration5-25 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TAC/GTA

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damageThe EMBO JournalNovember 20, 2025Alexandra Boegli, Elliott M Bernard, Louise Lacante, Gaël Majeux, Ella Hartenian, Vanessa Mack, Petr Brozbackbone, both the CMV promoter was replaced by an EF1a promoter using SnaBI-NheI (New England Biolabs #R0130S and #R3131S) restriction digest and InFusion cloning, as well as the Puromycin resistance cassette was

(2) Evaluation of Cas13d as a tool for genetic interaction mappingNat CommunFebruary 14, 2025Ghanem El Kassem, Jasmine Hillmer, Michael Boettcherusing Gibson Assembly44. The resulting plasmid was then digested with SacII and SnaBI (NEB, R0157S, R0130S). Gene fragment containing WPRE-3LTR with Gibson Assembly compatible overhangs was ordered from Genscript

(3) IRF4 requires ARID1A to establish plasma cell identity in multiple myelomaCancer CellJuly 8, 2024Arnold Bolomsky, Michele Ceribelli, Sebastian Scheich, Kristina Rinaldi, Da Wei Huang, Papiya Chakraborty, Lisette Pham, George W. Wright, Tony Hsiao, Vivian Morris, et al.gradient media,Cytiva,Cat.#17144002 BfuA1,New England Biolabs,Cat.#R0701L SnaB1,New England Biolabs,Cat.#R0130L Puromycin Dihydrochloride,Thermo Fisher Scientific,Cat.#A1113802 Geneticin™ Selective Antibiotic (G418

(4) Enhancing Antibody-Specific Productivity: Unraveling the Impact of XBP1s Overexpression and Glutamine Availability in SP2/0 CellsBioengineering (Basel)February 21, 2024Priscilla González-Pereira, Ryan Trinh, Alex Vasuthasawat, Angelo Bartsch-Jiménez, Constanza Nuñez-Soto, Claudia Altamiranorestriction digestion using the enzymes SalI (Thermo Fisher ER0641, Freemont, CA, USA) and SnaBI (NEB R0130S, Ipswich, MA, USA). The enzymes were applied sequentially in two steps due to distinct optimal reaction

(5) Novel Enhanced Mammalian Cell Transient Expression Vector via Promoter CombinationInternational Journal of Molecular SciencesFebruary 16, 2024SunKyung Yoon, SeJin Park, JuneWoo Lee, Byoungguk Kim, WonSeok Gwakwere treated with restriction enzymes Hinc II (R0103L, New England Biolabs, Ipswich, MA, USA)/SnaB I (R0130L, New England Biolabs, Ipswich, MA, USA). Subsequently, they were self-ligated and transformed to produce

(6) Response to Bruton’s tyrosine kinase inhibitors in aggressive lymphomas linked to chronic selective autophagyCancer CellFebruary 1, 2024James D. Phelan, Sebastian Scheich, Jaewoo Choi, George W. Wright, Björn Häupl, Ryan M. Young, Sara A. Rieke, Martine Pape, Yanlong Ji, Henning Urlaub, et al.Laemmli sample buffer,BioRad,Cat #1610747 BfuA1,New England Biolabs,Cat.#R0701L SnaB1,New England Biolabs,Cat.#R0130L Puromycin Dihydrochloride,Thermo Fisher Scientific,Cat.#A1113802 Geneticin™ Selective Antibiotic (G418

(7) LRP8-mediated selenocysteine uptake is a targetable vulnerability in MYCN-amplified neuroblastomaEMBO molecular medicineAugust 7, 2023Alborzinia H, Chen Z, Yildiz U, Freitas FP, Vogel FCE, Varga JP, Batani J, Bartenhagen C, Schmitz W, Büchel G, et al.sub‐cloned via restriction digest of the plasmid with NsiI‐HF (NEB, Cat. No. R3127) and SnaBI (NEB, Cat. No. R0130) and the insertion of a synthetic dsDNA fragment coding for a gRNA scaffold sequence with MS2 stem‐loop

(8) EtcPRS (Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondiiiScienceApril 21, 2023Sun P, Zhang Y, Wang C, Hu D, Liu J, Chen L, Shi F, Tang X, Hao Z, Suo J, et al.buffer,NEW ENGLAND Biolabs,B9027S Percoll,CYTIVA,17089101 SnaBI restriction enzyme,NEW ENGLAND Biolabs,R0130L 4% paraformaldehyde (PFA),NOVON,SS0312 RIPA,Macgene,MP015 DMSO,Applichem,A3672 0.25% Triton X-100,Sigma,MC0711 Albumin

(9) Genetic architecture and evolution of color variation in American black bearsCurrent BiologyJanuary 1, 2023Emily E. Puckett, Isis S. Davis, Dawn C. Harper, Kazumasa Wakamatsu, Gopal Battu, Jerrold L. Belant, Dean E. Beyer, Colin Carpenter, Anthony P. Crupi, Maria Davidson, et al.peptides, and recombinant proteins" BamHI,NEB,R0136L HindIII,NEB,R0104L XholI,NEB,R0146L SnaBI,NEB,R0130S Lipofectamine 2000,Invitrogen,11668-019 Hygromycin B,Research Products International,H75020-1.0 RPMI 1640 medium

(10) Genetic tools for the stable overexpression of circular RNAsRNA BiologyDecember 31, 2022Nicol Mecozzi, Arianna Nenci, Olga Vera, Ilah Bok, Aimee Falzone, Gina M. DeNicola, Florian A. KarrethGeneArt, followed by PCR amplification and InFusion cloning into SnaBI/PstI (New England Biolabs, Cat. #s R0130 and R3140, respectively) digested EF1α-spGFP-ZKSCAN1-Blast transposon plasmid. Cell culture Human

(11) System-Wide Analysis of the GATC-Binding Nucleoid-Associated Protein Gbn and Its Impact on Streptomyces DevelopmentmSystemsJune 28, 2022Chao Du, Joost Willemse, Amanda M. Erkelens, Victor J. Carrion, Remus T. Dame, Gilles P. van Wezelno. R3136), XbaI (R0145), EcoRI-HF (R3101), HindIII-HF (R3104), BbsI (R0539), NcoI-HF (R3193), SnaBI (R0130), StuI (R0187), SacI (R0156), and NdeI (R0111). Phusion polymerase (M0532) and T4 DNA ligase (M0202)

(12) Protocol for design, construction, and selection of genome phage (gPhage) display librariesSTAR ProtocolsDecember 17, 2021Luis Antonio Rodriguez Carnero, AndrÃ© Azevedo Reis Teixeira, Fenny Hui Fen Tang, AndrÃ©ia Kuramoto, Maria JÃºlia Manso Alves, Walter Colli, JoÃ£o Carlos Setubal, EdÃ©cio Cunha-Neto, Renata Pasqualini, Wadih Arap, et al.broth,Difco,Cat#262200 Carbenicillin,Sigma,Cat#C1389-5G Penicillin,Sigma,Cat#D7794-100MU Streptomycin,Sigma,Cat#S9137-100G CsCl,Sigma,Cat#V000561 Ethidium bromide,Thermo Fisher Scientific,Cat#15585-011 Eco105I,New England BioLabs,Cat#R0130L FastAP,Thermo Fisher Scientific,Cat#EF0651 Low-melting point agarose,Sigma,Cat#A9539-500G T4 DNA ligase

(13) Targeted Enrichment of rRNA Gene Tandem Arrays for Ultra-Long Sequencing by Selective Restriction Endonuclease DigestionFrontiers in Plant ScienceApril 28, 2021Anastasia McKinlay, Dalen Fultz, Feng Wang, Craig S. Pikaardthe restriction endonucleases BstZ17I-HF (NEB #R3594), SpeI-HF (NEB #R3133), BclI-HF (NEB #R3160), SnaBI (NEB #R0130), MscI (NEB #R0534), and PvuII-HF (NEB #R3151) at 37◦ C overnight. The buffer was then removed and replaced

(14) SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by AktDevelopmental cellApril 19, 2021Burckhardt CJ, Minna JD, Danuser G.puromycin resistance genes follow after an internal ribosomal entry site. For subcloning SnaBI (NEB, R0130) and NotI-HF (NEB, R3189) were used. The same approach was used for Dyn2-GFP, Eps15-GFP, Eps15-SNAP,

(15) Engineering SARS-CoV-2 using a reverse genetic systemNature ProtocolsMarch 1, 2021Xuping Xie, Kumari G. Lokugamage, Xianwen Zhang, Michelle N. Vu, Antonio E. Muruato, Vineet D. Menachery, Pei-Yong Shino. R0734L) Restriction enzyme PvuI-HF (NEB, cat. no. R3150S) Restriction enzyme SnaBI (NEB, cat. no. R0130L) Ribonucleotide solution mix (rNTP solution mix; NEB, cat. no. N0466L) SOC outgrowth medium (10 mL;

(16) Separable functions of Tof1/Timeless in intra-S-checkpoint signalling, replisome stability and DNA topological stressNucleic acids researchDecember 2, 2020Westhorpe R, Keszthelyi A, Minchell NE, Jones D, Baxter J.purified DNA was digested with BamHI-HF (New England Biolabs, R3136S) and SnaBI (New England Biolabs, R0130L) or SnaBI alone according to manufacturer's instructions. After nicking/digestion the DNA was precipitated,

(17) Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and ChickenSTAR ProtocolsOctober 28, 2020Martyna Lukoseviciute, Irving T.C. Ling, Upeka Senanayake, Ivan Candido-Ferreira, Gunes Taylor, Ruth M. Williams, Tatjana Sauka-SpenglerL4509CAS: 151-21-3 High Fidelity (HF) NcoI,NEB,Cat#R3193S SnaBI Restriction endonuclease,NEB,Cat#R0130 High Fidelity (HF) EcoRV,NEB,Cat#R3195 "Proteinase K, recombinant, PCR Grade",Roche,Cat#3115836001 "RNase A, DNase and protease-free (10 mg/mL

(18) Mechanical regulation of glycolysis via cytoskeleton architectureNatureFebruary 1, 2020Park JS, Burckhardt CJ, Lazcano R, Solis LM, Isogai T, Li L, Chen CS, Gao B, Minna JD, Bachoo R, et al.subcloned from pEGFP vectors into pLVX puro or neo (632183, 832181, Clontech) vectors using SnaBI (R0130, NEB) and NotI (R3189S) restriction sites. For shRNA mediated protein knockdown vectors from the pGIPZ

(19) A Novel Animal Model of Emphysema Induced by Anti-Elastin AutoimmunityJournal of immunology (Baltimore, Md. : 1950)July 15, 2019Gu BH, Sprouse ML, Madison MC, Hong MJ, Yuan X, Tung HY, Landers CT, Song LZ, Corry DB, Bettini M, et al.specific restriction sites digestion: BstBI (NEB #R0519) and MfeI (NEB #R3589) for TCRα, and SnaBI (NEB #R0130) and SacII (NEB #R0157) for TCRβ. Following confirmation of the correct insertion sites, Sanger Sequencing

(20) Rapid identification and expression of human TCRs in retrogenic mice☆Journal of Immunological MethodsDecember 1, 2016Maran L. Sprouse, Gabriele Blahnik, Thomas Lee, Natalie Tully, Pinaki Banerjee, Eddie A. James, Maria J. Redondo, Matthew L. Bettini, Maria Bettiniregion. Purified alpha nested PCR products and beta containing TCR vectors were digested with SnabI (NEB #R0130L) and SacII (NEB #R0157S), and ligated. Stable retroviral producer cell lines were generated as previously

Be the First to Write a Review!

SnaBI

SnaBI from New England Biolabs

Sequence

New England Biolabs

Citations (24)

Reviews

SnaBI from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map