SmaI from New England Biolabs

Product Specs
ItemSmaI
CompanyNew England Biolabs
Price $286.00 Inquire
Catalog NumberR0141L
Quantity10000 units
ApplicationsDNA Manipulation
Concentration20 u/µL
TypeRestriction Enzyme
Temperature-20 C

Sequence

CCC/GGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-Creminsdephosphorylated genomic DNA by incubating 5 μg DNA with 10 μL Cas9 RNPs, 10 mM dATP (Thermo Scientific#R0141), and 1 μL Taq polymerase (NEB#M0273) at 37°C for 60 min, followed by dA-tailing at 72°C for 5 min.

(2) Neuronal hyperactivity becomes mTORC1 independent due to transcriptional changes in tuberous sclerosis complex disease modelsCell ReportsDecember 23, 2025Wardiya Afshar-Saber, Juan F. Ruiz, Isabel Gisser, Ziqin Yang, Leena Mehendale, Nicole A. Teaney, Rachel Hobson, Madison R. Glass, Truc T. Pham, Elizabeth Bainbridge, et al.table). The backbone pLV-EF1A-IRES-Neo (Addgene, #85139) was digested with SmaI (New England Biolabs R0141S) and ClaI (New England Biolabs R0197S). These three fragments were cleaned up using SPRI beads (Mag-Bind

(3) Glutamic acid-lysine (EK) rich motif of RabD2 self-associates and regulates adhesion through multivesicular bodies in Entamoeba histolyticaBMC BiolOctober 15, 2025Navyaka Padavala, Kuldeep Vermaweight band (approximately 792 bp) was excised and digested with SmaI and XhoI restriction enzymes (R0141S, R0146S New England Biolabs, USA). The pEhTex-HA plasmid was also digested with the aforementioned enzymes

(4) Systematic over-expression of secondary metabolism transcription factors to reveal the pharmaceutical potential of Aspergillus nidulansCommun BiolOctober 9, 2025Shuhui Guo, Lakhansing Pardeshi, Longguang Qin, Chris Y. Cheung, Xiaofeng Liu, Lu Fan, Chi Cheng Mok, Chirag Parsania, Zhiqiang Dong, Ben C. B. Ko, et al.the Aspergillus fumigatus pyroA gene as the selection marker43, was linearized by SmaI (NEB, cat no. R0141) digestion. Fragments containing epitope tag, 3×HA or 3×FLAG, or 6×HIS and ADH1(t) were then amplified

(5) Deciphering the sequence basis and application of transcriptional initiation regulation in plant genomes through deep learningGenome BiologySeptember 22, 2025Pengfei Gao, Lijie Lian, Wanjie Feng, Yuxue Ma, Jieni Lin, Liya Qin, Shanmeng Hao, Haonan Zhao, Xuantong Liu, Jing Yuan, et al.amplified from Williams 82 genomic DNA and cloned into the pZHY930 vector using KpnI (R3142, NEB) and SmaI (R0141, NEB) restriction sites. Recombinant plasmids were validated by Sanger sequencing to confirm insert

(6) Highly quantitative measurement of differential protein-genome binding with PerCell chromatin sequencingCell Reports MethodsJune 16, 2025Alexi Tallan, Jack Kucinski, Benjamin Sunkel, Cenny Taslim, Stephanie LaHaye, Qi Liu, Jun Qi, Meng Wang, Genevieve C. Kendall, Benjamin Z. StantonPol + T4 PNK),NEB,Cat#E6051AAVIAL Klenow Fragment (3–5 exo-),NEB,Cat# M0212S 100 mM dATP,NEB,Cat#R0141 10 mM ATP,Fisher Scientific,Cat#10-297-018 2.5 mM dNTP mix,Fisher Scientific,Cat#10-297-018 Phusion

(7) Structural and systems characterization of phosphorylation on metabolic enzymes identifies sex-specific metabolic reprogramming in obesityMolecular CellJune 5, 2025Tigist Y. Tamir, Shreya Chaudhary, Annie X. Li, Sonia E. Trojan, Cameron T. Flower, Paula Vo, Yufei Cui, Jeffrey C. Davis, Rachit Mukkamala, Francesca N. Venditti, et al.Scientific,TS45950 Tert-Butyldimethylsilyl-N-methyltrifluoroacetamide with 1% tert-Butyldimethylchlorosilane (TBDMS),Millipore Sigma,375934 SmaI,New England Biolabs,R0141 HindIII,New England Biolabs,R0104 BsmBI-v2,New England Biolabs,R0739 Dulbecco’s Modified Eagle Medium

(8) LncRNA SLNCR phenocopies the E2F1 DNA binding site to promote melanoma progressionCell ReportsMay 27, 2025Kushani Shah, Eleni Anastasakou, Leinal Sejour, Yufei Wang, Leon Wert-Lamas, Christopher Rauchet, Sabine Studer, Simon Goller, Robert J. Distel, Wayne Marasco, et al.SLNCR fragments were ordered as gblocks from IDT and digested with SphI-HF (NEB, R3182S) and SmaI (NEB, R0141S) restriction enzymes in CutSmart buffer (NEB, B6004S). SLNCR fragment 1 was digested using SphI-HF only.

(9) Skin mycobiota-mediated antagonism against Staphylococcus aureus through a modified fatty acidCurrent BiologyMay 19, 2025Caitlin H. Kowalski, Uyen Thy Nguyen, Susannah Lawhorn, T. Jarrod Smith, Rebecca M. Corrigan, Won Se Suh, Lindsay Kalan, Matthew F. Barber,Selleck Chemicals,Cat#S4297 Oxacillin,Sigma-Aldrich,Cat#O1002 Phusion polymerase,NEB,Cat#M0530S T4 Polynucleotide Kinase,NEB,Cat#M0201S SmaI,NEB,Cat#R0141S EcoRV,NEB,Cat#R0195L XbaI,NEB,Cat#R0145S Novagen Pellet Paint Co-precipitant,EMB Millipore,Cat#69049-3 Quick

(10) Dynamics of stress-induced c-fos expression in the rat prelimbic cortex: lessons from intronic and mature RNA and protein analysesNeurobiology of StressApril 22, 2025Patricia Molina, Xavier Belda, Sandra Beriain, Sara Serrano, Gentzane Compte, Raül Andero, Antonio ArmarioJolla, CA), subcloned into a pBluescript SK-1 (Stratagene, La Jolla, CA) and linearized with SmaI (Ref. R0141S, New England BioLabs). The intronic c-fos probe was a kind gift from Dr Lin (California Institute of

(11) Mosaic H3K9me3 at BREACHes predicts synaptic gene expression associated with fragile X syndrome cognitive severitybioRxiv : the preprint server for biologyMarch 19, 2025Pham K, Malachowski T, Zhou L, Kim JH, Su C, Phillips-Cremins JE.the 5 μg of dephosphorylated DNA and 10 μL of the RNPs mix along with 10 mM dATP (Thermo Scientific, R0141), and 1 μL Taq polymerase (New England Biolabs, M0273) at 37°C for 60 minutes and allowed for dA-tailing

(12) Plasmid Library Construction From Genomic DNACurr ProtocJanuary 22, 2025Valeria Florez-Cardona, Jessica Khani, Emily McNutt, Bruno Manta, Mehmet Berkmen12145)Nuclease free water (NF‐water, NEB, cat. no B1500)Blunt‐end restriction enzyme: e.g., SmaI (NEB, cat. no. R0141S)Sterile LB liquid medium (Thermo Fisher, cat. no. L3522‐1KG)10× CutSmart Buffer (NEB, cat. no B6004)Alkaline

(13) CRISPR targeting of mmu-miR-21a through a single adeno-associated virus vector prolongs survival of glioblastoma-bearing miceMolecular TherapyJanuary 8, 2025Lisa Nieland, Anne B. Vrijmoet, Isabelle W. Jetten, David Rufino-Ramos, Alexandra J.E.M. de Reus, Koen Breyne, Benjamin P. Kleinstiver, Casey A. Maguire, Marike L.D. Broekman, Xandra O. Breakefield, et al.61591; https://www.addgene.org/61591/)15 after a digest using the restriction enzymes SmaI (catalog: R0141; New England Biolabs) and HindIII (catalog: R0104; New England Biolabs) followed by Gibson assembly

(14) RNA degradation triggered by decapping is largely independent of initial deadenylationThe EMBO JournalSeptember 25, 2024Léna Audebert, Frank Feuerbach, Mostafa Zedan, Alexandra P Schürch, Laurence Decourty, Abdelkader Namane, Emmanuelle Permal, Karsten Weis, Gwenaël Badis, Cosmin Saveanu,New England Biolabs,R0524 NotI-HF,New England Biolabs,R3189 SstII,ThermoFisher,45230-018 SmaI,New England Biolabs,R0141 XhoI,New England Biolabs,R0146 Lysyl endopeptidase (Lys-C),Fujifilm Wako chemicals,125-05063 "Trypsin gold, mass spectrometry grade

(15) Mechanisms controlling replication fork stalling and collapse at topoisomerase 1 cleavage complexesMolecular CellSeptember 19, 2024Rose Westhorpe, Johann J. Roske, Joseph T.P. Yeeles,New England Biolabs,Cat# R0644 NheI-HF®,New England Biolabs,Cat# R3131 SmaI,New England Biolabs,Cat# R0141 BlpI,New England Biolabs,Cat# R0585 T4 PNK,New England Biolabs,Cat# M0201 Sepharose™ 4B,Sigma Aldrich,Cat#

(16) Structural and systems characterization of phosphorylation on metabolic enzymes identifies sex-specific metabolic reprogramming in obesitybioRxivSeptember 1, 2024Tigist Y Tamir, Shreya Chaudhary, Annie X Li, Sonia E Trojan, Cameron T Flower, Paula Vo, Yufei Cui, Jeffrey C Davis, Rachit S Mukkamala, Francesca N Venditti, et al.Scientific,122-3832UI Plasmids and cloning,, SuperPiggyBac Transposase,SystemBio,PB210PA-1 SmaI,New England Biolabs,R0141 HindIII,New England Biolabs,R0104 BsmBI-v2,New England Biolabs,R0739 pUC19 backbone,Thermo Fisher Scientific

(17) Single-molecule imaging reveals the mechanism of bidirectional replication initiation in metazoaCellJuly 25, 2024Riki Terui, Scott E. Berger, Larissa A. Sambel, Dan Song, Gheorghe ChistolR3104S NcoI-HF,NEB,Cat# R3193S NdeI,NEB,Cat# R0111S NotI-HF,NEB,Cat# R3189S SalI-HF,NEB,Cat# R3138S SmaI,NEB,Cat# R0141S SpeI-HF,NEB,Cat# R3133S SphI-HF,NEB,Cat# R3182S XbaI,NEB,Cat# R0145S XhoI,NEB,Cat# R0146S Klenow

(18) DdmABC-dependent death triggered by viral palindromic DNA sequencesCell ReportsJuly 23, 2024William P. Robins, Bradley T. Meader, Jonida Toska, John J. Mekalanosusing DNA Repair Mix (NEB E6050L) and shotgun cloned into pWR1566 that had been cut with SmaI (NEB R0141L) and AvrII (NEB R0174L), blunted using DNA Repair Mix, and dephosphorylated using Antarctic Phosphatase

(19) A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeastPLOS GeneticsJune 4, 2024Sonia Borao, Montserrat Vega, Susanna Boronat, Elena Hidalgo, Stefan Hümmer, José AytécDNA, respectively, and the pAY24 plasmid digested with PstI and SmaI (New England BioLabs, #R0140 and #R0141, respectively). The sequence of all constructs was confirmed by Sanger sequencing at the Genomic Core

(20) A synthetic biology approach to assemble and reboot clinically relevant Pseudomonas aeruginosa tailed phagesMicrobiology SpectrumMarch 5, 2024Thomas Ipoutcha, Ratanachat Racharaks, Stefanie Huttelmaier, Cole J. Wilson, Egon A. Ozer, Erica M. Hartmannfrom the yeast recombination matrix were performed using 10 µg of yeast DNA digested using SmaI (NEB, R0141S) for JG024 and ScaI (NEB, R3122S) for DMS3, vB_PaeP_PAO1_Ab05, and F8. Restriction enzymes were inactivated

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SmaI

SmaI from New England Biolabs

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New England Biolabs

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SmaI from New England Biolabs

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