psiCHECK-2 Vector from Promega

Product Specs
ItempsiCHECK-2 Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberC8021
Hostmammalian cell line of choice
PromoterSV40 early enhancer/promoter
ResistanceAmp
Quantity20microg
Molecular Weight~78kDa
Vector NamepsiCHECK-2

Specifications/Features

Vector Size:
~6.3 kb

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) CircSMAD4 shapes matrix-remodeling TAMs in lung adenocarcinomaNon-coding RNA ResearchAugust 1, 2026Zhengwei Yu, Xinyue Wang, Yiqian Zheng, Yifan He, Jiayu Lin, Yue Xiao, Bin Mo, Haoyu Xie, Sitong Hang, Xia Gao, et al.into the pmirGLO Dual-Luciferase vector (Promega, Cat# E1330) or the psiCHECK-2 vector (Promega, Cat# C8021). Corresponding seed-site mutants and m^6A-site mutants were generated using the Mut Express II Fast

(2) ATF6 ameliorates renal warm ischemia-reperfusion injury through FHL2 -mediated NF-κb signaling pathwayiScienceApril 17, 2026Xuan Wu, Ji-Hua Shi, Yang Bai, Dong-Jing Yang, Meng-Yao Jia, Dan-Feng Guo, Jia-Kai Zhang, Xiao-Yi Shi, Hua-Peng Zhang, Wen-Zhi Guo, et al.promoter region DNA sequences of the human FHL2 gene were cloned into the psiCHECK-2 plasmid vector (C8021, Promega, Beijing, China). The constructed plasmids were then transfected into 293T cells. Luciferase

(3) The birth, death, and evolutionary compensation of uORFs in DrosophilaNucleic Acids ResMarch 26, 2026Mengze Xu, Chenlu Liu, Wanting Jin, Yuanqiang Sun, Yuange Duan, Xiaolu Tang, Jian LuD. melanogaster Schneider line 2 (S2) cell line, we used a reporter plasmid modified from the psiCHECK-2 vector (C8021, Promega). This modified reporter plasmid contains the promoter of the actin5C gene (−522 to −1 region)

(4) Functional siRNA Delivery via Jet Nebulization: Proof-of-Concept IL-1ß Silencing in Macrophage-like THP-1 CellsInt J Mol SciMarch 23, 2026Duy Bao Tran Nguyen, Ahmed S. M. Ali, Dongwei Wu, Johanna Berg, Daniel C. Lauster, Jens Kurreck, Beatrice TolksdorfUSA) and sequences were inserted downstream of the Renilla luciferase gene of the psiCHECK™ 2 vector (C8021, Promega, Madison, WI, USA) plasmid using the XhoI/NotI restriction sites in the MCS, resulting in psiCHECK™-2

(5) BOLL ‐Containing Aggregates Mediate the Translational Regulation During Human OogenesisCell ProliferationFebruary 25, 2026Ying Li, Lingya Mao, Boyuan Liang, Longxin Xie, Wenpei Xiang, Kehkooi Keeto the sequences downloaded from the NCBI website, were cloned into the psiCHECK2 vector (Promega, C8021). One day before transfection, 293FT cells were transferred to a well in a 24‐well plate at a density

(6) Curcumin, the Bioactive Compound of Turmeric, Boosts Cellular Antioxidant Defense via the miR-22-3p/MCAT AxisFoodsFebruary 12, 2026Haiqi Wang, Yanqi Lin, Yuanyuan Li, Shiying Huang, Guiling Li(Takara Bio, Beijing, China) and subcloned into the corresponding sites of the psiCHECK-2 vector (Cat# C8021, Promega, Madison, WI, USA) via T4 DNA Ligase (Cat# 2011A, Takara Bio). Separately, to generate the

(7) MiR-21 modulates P.g-LPS induced apoptosis and inflammatory response in HUVECs via NF-κB/iNOS/NO pathway by targeting PDCD4Non-coding RNA ResearchFebruary 1, 2026Jing Ren, Huiqiong Zou, Siyu Sun, Shan Chen, Rui He, Qianbing Zhou, Jun Tao, Junying Yangsequences of PDCD4 were amplified and subcloned into the psiCHECK™-2 luciferase reporter vector (Promega, C8021). Human embryonic kidney cells (HEK-293T) were then co-transfected with these luciferase reporter constructs

(8) Wolbachia-derived small non-coding RNAs exhibit cross-kingdom regulatory effects on host reproduction of a polyphagous miteiScienceJanuary 16, 2026Yi-Yin Zhang, Xin-Yu Shen, Dian-Shu Zhao, Ary Hoffmann, Xiao-Yue Hongmethod details,N/A,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, psiCHECK2,Promega,Cat# C8021 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, miRanda,Enright et

(9) RNAi Regulator C3PO Promotes Arbovirus Infection in Insect VectorsAdvanced ScienceJanuary 1, 2026Yan Xiao, Tianyu Guan, Qianfeng Xia, Chen Chen, Qian Wang, Lan Luo, Hong Lu, Feng CuiAssay ≈300 bp fragment around the target site of NHLRC2 was inserted into the Psi‐CHECK2 vector (Promega; C8021) between the restriction sites NotI and XhoI to generate recombinant plasmids. Mutations in the target

(10) IGF1R-targeted delivery of a bridged nucleic acid oligonucleotide-peptide conjugate for microRNA-21 inhibition in triple-negative breast cancerNAR CancerDecember 31, 2025Yuanyuan Jin, Vilas S Desai, Jason D Mazzaroth, Eric Wickstromday before transfection. Cells were co-transfected with 100 ng of dual-luciferase psiCHECK2 plasmid (C8021, Promega) containing a perfect complement to miR-21 in the 3′UTR of the Renilla luciferase gene, and

(11) Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalkPLOS PathogensDecember 31, 2025Li Fan, Yang Sun, Fangzhou Lou, Zilong Fang, Wenxiang Ding, Xiangxiao Li, Yan Li, Qingqing Shen, Siyu Deng, Jihuan Liang, et al.the multiple cloning site of the psiCheck-2 synthetic firefly luciferase reporter plasmid (Promega, #C8021). Plasmid integrity was verified through DNA sequencing. RAW264.7 cells were seeded in 48-well plates

(12) Sevoflurane attenuates hypoxia/reoxygenation-induced cardiomyocyte injury via the LncRNA FAM201A/miR-488-3p/PTEN axisBMC AnesthesiologyDecember 27, 2025Chen Yang, Yuanzhi Li, Qiang Li, Xibao Shi, Jinping Li, Ying Chen, Huiling Qinfragments of the target genes were synthesized by GenScript and then cloned into the psiCHECK2 vector (Cat# C8021, Promega, Madison, WI, USA). H9c2 cells were co-transfected with the recombinant plasmid and miR-488-3p

(13) A 3' UTR polymorphism g.1618 G > A in the MAFA gene modulates miR-3678-3p binding and enhances meat production in sheep via the MAFA/GHR/JAK2 pathwayGenet Sel EvolDecember 22, 2025Cuiyu Lai, Dandan Tan, Xuewen Han, Yu Fu, Jinlin Chen, Xiaofan Yang, Xuesong Shan, Yang Chen, Huaizhi Jiangor A allele at position g.1618 was synthesized (GenScript) and cloned into the Psicheck2.0 vector (C8021, Promega, USA). Plasmids (1 μg/μL) carrying either MAFA-G or MAFA-A, together with 40 pmol of miR-3678-3p

(14) MicroRNA-29b-3p promotes endothelial cell inflammation via NTRK2/PI3K/NLRP3 pathway in Moyamoya diseaseEur J Med ResDecember 1, 2025Liangzhe Wei, He Ren, Yuanwei Lin, Xinpeng Deng, Yuchun Liu, Jingjing Zeng, Jinghui Lin, Xiang Gao, Jie Sun, Yi Huangdatabase analysis. The target sequences of WT or MUT were cloned into the pSI-Check2 reporter vector (C8021, Promega Corporation, WI, USA), and then, the vector and miR-29b-3p mimic or negative control (NC) mimic

(15) CircAASS alleviates renal injury and fibrosis by regulating mitochondrial homeostasis in tubular epithelial cellsAutophagyNovember 20, 2025Tongtong Ma, Yanmei Yu, Huasheng Luo, Ziqi Zhang, Miaotao Wei, Chunjie Tian, Xianmou Fan, Zhenyi Yan, Shaowu Zhang, Junfeng Hao, et al.circAASS and the 3’ UTR of PINK1 were cloned into the psi-CHECK2 dual-luciferase reporter vector (Promega, C8021). To further validate the specific binding of MIR324-3p to these sequences, we generated mutant constructs

(16) DICER1 hotspot mutation induces 3p microRNA gain of function via Argonaute strand switchNature Structural & Molecular BiologyNovember 4, 2025Sharan Malagobadan, Chunmei Shi, Acong Yang, Indranil Mondal, Habikah Baldeh, Karrie Spain, Wilfried Guiblet, Shuo Guv.2.14.0. Reporter assay Dual luciferase reporter assays were performed using a psiCHECK-2 plasmid (Promega, C8021), in which the target sequence for miRNA of interest was cloned into the 3′ UTR of the Renilla luciferase.

(17) A portable poison exon for small-molecule control of mammalian gene expressionNature MethodsNovember 3, 2025Qian Hou, Maxim Oleynikov, Xiaohan Mei, Linghao Dong, Timo Hagen, Samie R. JaffreyExtraction Kit (catalog number 28706). The psiCHECK-2 vector was purchased from Promega (catalog number C8021). pINDUCER20 (Plasmid #44012), pcDNA3-EGFP (Plasmid #13031), pcDNA3-mCherry (Plasmid #30125), pCMV-Cre

(18) A microRNA CRISPR screen reveals microRNA-483-3p as an apoptotic regulator in prostate cancer cellsCell Death DisOctober 24, 2025Jonathan Tak-Sum Chow, Ayeisha Desjardins, Daniel K. C. Lee, Iulia A. Grigore, Linda Lee, Norman J. Fu, Stephanie Chau, Byeong Yeop Lee, Martino Marco Gabra, Leonardo SalmenaLSB-hsa-miR-483-3p (Addgene #103550) and all 3’UTR sequences were cloned into the psiCHECK-2 vector (Promega, C8021). Lentivirus was made using the pMDG.2 (Addgene #12259) and psPAX2 (Addgene #12260) vectors. Plasmid cloning

(19) Multi-omics analyses integration reveals a novel TRF2-miR-181a-5p-S100A10 regulatory axis in colon cancerCell Mol Biol LettOctober 20, 2025Roberto Dinami, Eleonora Petti, Paola Ostano, Sara Iachettini, Angela Rizzo, Carmen Maresca, Pasquale Zizza, Serena Di Vito, Manuela Porru, Carmen D'Angelo, et al.electrophoresis, purified from the gel by commercial kit (Qiagen), and then, cloned into psiCHECK-2 vector (#C8021; Promega) as previously described [9]. The mutagenesis of the binding site of miR-181-5p on the 5′UTR

(20) Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biologyFront ToxicolOctober 1, 2025Alexandra Nessar, Viola Röhrs, Mathias Ziersch, Ahmed S. M. Ali, Julia Moradi, Anke Kurreck, Johanna Berg, Jens Kurreckdual-luciferase reporter assay. For this purpose, a reporter construct based on the psiCHECK™-2 vector (C8021, Promega, Madison, WI, United States) was used, in which the Renilla luciferase (RLuc) gene is fused

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