Fig 1: (A) Setd2 mRNA levels assessed by RT-qPCR 48 h post transfection. All data are normalised to Ct values for Actb. Data are given as mean 2−ΔΔCt values ± SEM of three independent experiments. ***P < 0.001 compared with Scrambled group by unpaired t-test. Scr, scrambled control; KD, knockdown. (B) Western blot of whole cell lysate showing effective depletion of H3K36me3 upon knockdown of Setd2. Total histone H3 levels are unaffected. ACTB was used as a loading control. Untr, untreated cells; Lipo, transfection vehicle only; Scr, scrambled control; KD, knockdown. (C) RNA-seq heatmap of significant differentially expressed transcripts 48 h post transfection. Values are given as row-wise standard-normalised fragments per kilobase of transcript per million mapped reads (z-score). Scr, scrambled control; KD, knockdown. (D) Top ten upregulated biological processes determined by GO analysis (PANTHER). See Supplementary Table S6 for a complete list of GO ID terms. (E) Top ten downregulated biological processes determined by GO analysis (PANTHER). See Supplementary Table S7 for a complete list of GO ID terms.