SfiI from New England Biolabs

Product Specs
ItemSfiI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0123L
Concentration20 u/µL
Quantity15000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GGCCN4/NGGCC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Synthetic budding morphogenesis by optogenetic receptor tyrosine kinase signalingbioRxivApril 2, 2026Louis S. Prahl, Ronald Canlla, Aria Zheyuan Huang, Daniel S. Alber, Sandra L. Shefter, Sachin N. Davis, Samuel H. Grindel, Zikang Dennis Huang, Thomas R. Mumford, William Benman, et al.New England Biolabs). CLPIT retroviral vector was obtained by double restriction digest with NotI-HF® and SfiI (#R0123S, New England Biolabs). Vectors were purified by gel electrophoresis and extracted using a Zymoclean Gel DNA Recovery kit

(2) Multiomics Analysis across the Life Cycle Identifies Zn 2 Cys 6 _61 as a Target for Enhancing Triterpenoid Production in Ganoderma lucidumJournal of Agricultural and Food ChemistryApril 1, 2026Yihong Li, Liwei Liu, Miaoqing Li, Jing Xu, Jihong Yang, Xinyu He, Wan Yang, Wei Li, Rui Zhang, Lisa Mao, et al.amplifying the coding sequence of Zn2Cys6_61 and cloning it into the pGADT7 vector digested with Sfi I (R0123S, New England Biolabs) and BamH I (R0136S, New England Biolabs). The recombinant prey plasmid was transformed

(3) m6A RNA methylation modulates antiviral response in celiac diseaseGenes & ImmunityJanuary 10, 2026Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Izei Pascual-Gonzalez, Luis Manuel Mendoza, Maria Legarda, Carlos Tutau, Luis Bujanda, Sankar Ghosh, Izortze Santin, Thomas S. Postler, et al.PCR product was cloned into the IRF7 overexpression vector using BsmBI (NEB #R0580) and SfiI (NEB #R0123S) restriction enzymes. The primers used for mutagenesis and cloning are listed in the Supplementary Table

(4) CD8 co-receptor modulates the specificity profile of the 1G4 TCR against NY-ESO-1Molecular TherapyJanuary 7, 2026Heather F. Jones, Zita E.H. Aretz, Ron S. Gejman, Tara Cicic, David A. ScheinbergCGACTCACTATAGGGCCGTATTGGCC and AGTGATTTCCGGCCTGTTTGGCC. PCR sequences were digested with SfiI enzyme (New England Biolabs #R0123L) at 50°C. Minigenes were then purified with the MinElute PCR Purification Kit (Qiagen #28004). PresentER

(5) One-step approach producing barcoded rabies virus with optimized diversityCell Reports MethodsDecember 15, 2025Kang Tan, Zi-xuan Shen, Ya-qian Wang, Yi-jun Zhu, Xiao-feng Wei, Hua-tai Xuinitiate assembly, the rabies viral vector pSAD-B19ΔG or pCVS-N2cΔG was linearized using SfiI (NEB, R0123L) at 50°C for 3 h and then purified using 0.5X DNA clean beads (VAHTS, N411-01). For assembly, 3 μg linearized

(6) Development and application of a barcoded rabies viral tracing method for mapping brain-wide inputs to single neuronsCell Reports MethodsDecember 15, 2025Kang Tan, Ya-qian Wang, Rong-rong Yang, Zi-xuan Shen, Liu Fan, Yi-jun Zhu, Chun Xu, Hua-tai XupSADΔG-EGFP or pCVSΔG-mRuby were linearized using the restriction enzyme SfiI (New England Biolabs, product R0123L) at 50°C for three hours and subsequently purified using VAHTS 0.5X DNA Clean Beads (product N411-01). For

(7) Generation of a scFv Derived from an IgM-Producing Hybridoma for the Detection of REST Expression in Premalignant Lesions and Invasive Squamous Cell CarcinomaInt J Mol SciDecember 11, 2025Cynthia Rodríguez-Nava, Karen Cortés-Sarabia, Lidia Riaño-Umbarila, Baltazar Becerril-Luján, Yolanda Medina-Flores, Olga Mata-Ruíz, Lourdes Lloret-Sánchez, Berenice Illades-Aguiar, Luz Del Carmen Alarcón-Romero, Carlos Ortuño-Pinedapurified and cut with the enzymes SfiI and NotI (both from New England Biolabs, Ipswich, MA, USA, Cat# R0123S and Cat# R0189S), and ligated to the plasmid pSyn1 previously cut with the same restriction enzymes

(8) Intrinsically disordered regions facilitate target search to drive promoter selectivity by a yeast transcription factorNature CommunicationsDecember 10, 2025Nir Strugo, Carmit Burstein, Sk Saddam Hossain, Noam Nago, Hadeel Khamis, Ariel KaplanHofmann) as a template (primers listed in Supplementary Table 15). The product was digested with SFI-I (R0123S; NEB). In all other experiments, BR is a 60 bp AT-rich sequence flanking a single Msn2 binding motif

(9) Neutralization of Crimean-Congo hemorrhagic fever pseudotyped virions with heavy chain antibodiesVirologica SinicaDecember 4, 2025Kewen Qian, Yu Zhang, Zhihao Li, Wei Ye, Yue Cui, Zheng Zhu, Zhengshan Chen, Jianrong Wang, Jin Han, Ping Huang, et al.amplified VHH fragments and pcomb3X vector were digested with SfiI restriction enzyme (Catalog No. R0123L, NEB, Ipswich, USA), ligated using T4 DNA ligase (Catalog No. CYHF0101-01-AB, Vazyme, Nanjing, China),

(10) A unified mechanism for mitochondrial damage sensing in PINK1-Parkin–mediated mitophagyThe EMBO JournalNovember 20, 2025Julia A Thayer, Jennifer D Petersen, Xiaoping Huang, Luiza M Gruel Budet, James Hawrot, Daniel M Ramos, Shiori Sekine, Yan Li, Michael E Ward, Derek P Narendra,New England Biolabs,R0146 BsmBI-v2,New England Biolabs,R0739 XbaI,New England Biolabs,R0145 SfiI,New England Biolabs,R0123 EcoRI-HF,New England Biolabs,R3101 Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit (Mitolite),AAT Bioquest

(11) Atypical contribution of caspase-3 to melanoma cancer cell motility by regulation of coronin 1B activityCell Death DisOctober 6, 2025Kevin Berthenet, Serena Diazzi, Catherine Jamard, Kinga B. Stopa, Clara Gil, Stefan Dragan, Deborah Fanfone, Trang TM. Nguyen, Nathalie Al, Francois Virard, et al.primers listed in table S1. PCR fragments and the linearized pITR2 vector were digested with SfiI (NEB, R0123S), purified, ligated and transformed into Stbl3 bacteria as previously described. To enable inducible

(12) A method for the detection and enrichment of endogenous cereblon substratesCell chemical biologyAugust 21, 2025Lloyd HC, Li Y, Payne NC, Zhao Z, Xu W, Kroupova A, Zollman D, Long T, Kabir F, Chen M, et al.200550). The randomly mutagenized insert and pCOMB3xSS vector were digested by SfiI (New England Biolabs R0123S) and the backbone gel extracted using the Monarch gel extraction kit (New England Biolabs T1020S). The

(13) Rationally designed self-assembled peptide nanofibers provoke robust humoral immunity against nervous necrosis virusJournal of VirologyAugust 19, 2025Chen Zhang, Yong-Can Zhou, Wen-Ye Song, Xin-Xin Liu, Hai-Hua Peng, Yun SunscFv fragments, and sdAb fragments underwent double digestion with NotI-HF (NEB, R3189) and SfiI (NEB, R0123). Digested scFv fragments were ligated into pCantab5E using T4 DNA ligase. Recombinant plasmids were

(14) Iterative deep learning design of human enhancers exploits condensed sequence grammar to achieve cell-type specificityCell SystemsJuly 16, 2025Christopher Yin, Sebastian Castillo-Hair, Gun Woo Woo Byeon, Peter Bromley, Wouter Meuleman, Georg SeeligGibson overhangs; digested and gel purified the plasmid backbone (pMPRA1, Addgene #49349) with SfiI (NEB R0123) to remove its promoter and reporter sequence; then inserted the library cassette into the digested

(15) Conformational ligand-directed targeting of calcium-dependent receptors in acute traumaMedJuly 11, 2025Renata Pasqualini, Christopher Markosian, Daniela I. Staquicini, Andrey S. Dobroff, Esteban Dodero-Rojas, Paul C. Whitford, E. Magda Barbu, Julianna K. Bronk, Marina Cardó-Vila, Dawn R. Christianson, et al.double-stranded DNA. The fUSE5 plasmid was digested with the SfiI restriction enzyme (New England Biolabs, Cat#R0123L), purified by using the QIAquick PCR Purification Kit (QIAGEN, Cat#28106), and eluted in 50 μL of 10

(16) Neutralization of the Pandemic Influenza A/H1N1 Virus with Lama glama Humanized Nanobodies (VHH)Antibodies (Basel)May 16, 2025Zeila Yazmín Páez-Hernández, Jose Luis Stephano-Hornedo, Jose Alberto Bolaños-Prats, Iván Córdova-Guerrero, Mariana Macías-Alonso, Joaquín G. Marrero, Angel Pulido Capiz, Victor García Gonzálezinserted in the linearized pCOMB3X (Addgene, Cat. #63890) vector using the endonuclease SfiI (NEB, Cat. R0123S) sites and chemo transformed into E. coli Top10F’. 4.5. Selected VHH Expression and Purification The

(17) A method for the detection and enrichment of endogenous cereblon substratesbioRxivMarch 29, 2025Hannah C. Lloyd, Yuli Li, N. Connor Connor Payne, Zhenguang Zhao, Wenqing Xu, Alena Kroupova, David Zollman, Tengfang Long, Farah Kabir, Mei Chen, et al.200550). The randomly mutagenized insert and pCOMB3xSS vector were digested by SfiI (New England Biolabs R0123S) and the backbone gel extracted using the Monarch gel extraction kit (New England Biolabs T1020S). The

(18) Discovery and preclinical development of a SdAb-based CAR-T technology for targeting CD33 in AMLMolecular Therapy OncologyMarch 20, 2025Franco Bernasconi-Bisio, Eva Molina, Vianca Ibarra, Inés Ibáñez-Sala, Federica Rochira, Patricia Jauregui, Saray Rodríguez-Diaz, Rebeca Martínez-Turrillas, Iñigo Azagra-Barber, Nuria Gómez-Cebrián, et al.with the Kapa TAQ kit (Roche #KK1015). The SdAb DNA repertoire was digested with SfiI enzyme (NEB #R0123L) and ligated into SfiI digested and rSAP (NEB# M0371L) dephosphorylated pCOMB3XSS phagemid vector (RRID:Addgene_63890)

(19) Massively parallel variant-to-function mapping determines functional regulatory variants of non-small cell lung cancerNat CommunFebruary 6, 2025Congcong Chen, Yang Li, Yayun Gu, Qiqi Zhai, Songwei Guo, Jun Xiang, Yuan Xie, Mingxing An, Chenmeijie Li, Na Qin, et al.products were cloned into pMPRA1 (MiaoLingBio, P31645) plasmid which was digested with SfiI-HF (NEB, R0123) in advance. Then the ligated library was electroporated into DH10B-Plus Electroporation-Competent Cell

(20) Iterative deep learning-design of human enhancers exploits condensed sequence grammar to achieve cell type-specificitybioRxivNovember 19, 2024Christopher Yin, Sebastian Castillo Hair, Gun Woo Byeon, Peter Bromley, Wouter Meuleman, Georg SeeligGibson overhangs; digested and gel purified the plasmid backbone (pMPRA1, Addgene #49349) with SfiI (NEB R0123) to remove its promoter and reporter sequence; then inserted the library cassette into the digested

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SfiI

SfiI from New England Biolabs

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New England Biolabs

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SfiI from New England Biolabs

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