SfcI from New England Biolabs

Product Specs
ItemSfcI
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0561S
Concentration2 u/µL
Quantity200 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

C/T(AG)(CT)AG

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Somatic hypermutation patterns are shaped by both motif position and sequence grammarThe EMBO JournalDecember 11, 2025Bianca Bartl, Ursula E Schoeberl, Renan Valieris, Johanna Fitz, Konstantin Roeder, Kutti R Vinothkumar, Benjamin Gundinger, Israel Tojal Da Silva, Rushad Pavri,Fisher Scientific,15240062 b-Mercaptoethanol,Invitrogen,21985023 Phenol:Chlorophorm:Isoamyl Alcohol,Invitrogen,15593049 Glycogen,Thermo Scientific,R0561 DNaseI,New England Biolabs,M0303S UltraPure low-melting Point agarose,Invitrogen,16520050 Proteinase

(2) GlycoRNA-L and glycoRNA-S mediate human monocyte adhesion via binding to Siglec-5Biochimica et Biophysica Acta (BBA) - Molecular Cell ResearchOctober 1, 2025Yong Li, Yisong Qian, Evan Huang, Zain Schwarz, Hannah Tai, Katherine Tillock, Tianhua Lei, Xiaofeng Yang, Mingui Fu(Thermo Fisher Scientific, Cat#:65001) per reaction were blocked with 50 ng/μL glycogen (Thermo Fisher Scientific, Cat#:R0561) and 1 U/μL RNase Inhibitor (NEB) in Biotin Wash Buffer (10 mM Tris HCl pH 7.4, 1 mM EDTA, 100 mM NaCl,

(3) ANP32E drives vulnerability to ATR inhibitors by inducing R-loops-dependent transcription replication conflicts in triple negative breast cancerNat CommunMay 17, 2025Sara Lago, Vittoria Poli, Lisa Fol, Mattia Botteon, Federica Busi, Alice Turdo, Miriam Gaggianesi, Yari Ciani, Giacomo D'Amato, Luca Fagnocchi, et al.MABE1095). Next, the complexes were captured on ProteinA/G agarose beads (Thermo Fisher Scientific R0561), washed, eluted, and purified by phenol/chloroform extraction. qPCR of control regions was performed

(4) Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragilityiScienceNovember 15, 2024Herrera-Moyano E, Porreca RM, Ranjha L, Skourti E, Gonzalez-Franco R, Stylianakis E, Sun Y, Li R, Saleh A, Montoya A, et al.each). Digested nucleic acids were EtOH-precipitated with 1.5 μl of glycogen (Thermo Fisher Scientific, R0561) and gentle resuspended in 50 μl of 1x TE buffer. 8 μg of digested nucleic acids were treated or not

(5) Deep learning-enhanced R-loop prediction provides mechanistic implications for repeat expansion diseasesiScienceAugust 16, 2024Jiyun Hu, Zetong Xing, Hongbing Yang, Yongli Zhou, Liufei Guo, Xianhong Zhang, Longsheng Xu, Qiong Liu, Jing Ye, Xiaoming Zhong, et al.and recombinant proteins" ,, Proteinase K,Sigma-Aldrich,Cat# 124568 Glycogen,Thermo Fisher,Cat# R0561 HindIII - HF,NEB,Cat# R3104 BsrGI - HF,NEB,Cat# R3575 XbaI,NEB,Cat# R0145 SspI - HF,NEB,Cat# R3132 RNase H

(6) Updated Pseudo-seq Protocol for Transcriptome-Wide Detection of PseudouridinesBIO-PROTOCOLMay 5, 2024Yi Pan, Hironori Adachi, Xueyang He, Jonathan L. Chen, Yi-Tao Yu, Paul L. Boutz49553-93-7)TRIzol reagent (Invitrogen, catalog number: 15596018)Glycogen (Thermo Scientific, catalog number: R0561)Oligo d(T)25 magnetic beads (New England Biolabs, catalog number: S1419S)T4 PNK (Thermo Scientific,

(7) Bioorthogonal labeling and profiling of N6-isopentenyladenosine (i6A) modified RNANucleic Acids ResearchApril 12, 2024Yuanyuan Li, Hongling Zhou, Shasha Chen, Yinan Li, Yuyang Guo, Xiaoqian Chen, Sheng Wang, Li Wang, Youfang Gan, Shusheng Zhang, et al.New England Biolabs (USA). Transcript Aid T7 High Yield Transcription kit (product # K0441) and Glycogen (product # R0561) were purchased from Thermo Fisher Scientific. Pyrobest™ DNA Polymerase and PrimeSTAR HS DNA Polymerase

(8) Supramolecular CRISPR-OFF switches with host–guest chemistryNucleic Acids ResearchFebruary 22, 2022Wei Xiong, Xingyu Liu, Qianqian Qi, Huimin Ji, Fengbo Liu, Cheng Zhong, Simin Liu, Tian Tian, Xiang ZhouInc. (USA). Transcript Aid T7 High Yield Transcription kit (product# K0441) and Glycogen (product# R0561) were purchased from Thermo Fisher Scientific Inc. Chemical synthesis Experimental details for synthesis

(9) FACS-based isolation of fixed mouse neuronal nuclei for ATAC-seq and Hi-CSTAR ProtocolsSeptember 17, 2021Ekaterina Eremenko, Anastasia Golova, Daniel Stein, Monica Einav, Ekaterina Khrameeva, Debra Toiberbuffer,NEB,B7202S Chloroform,Frutarom,5551030 Phenol,Bio-Lab ltd,0016912344006 Glycogen,Sigma-Aldrich,R0561 T4 Polynucleotide Kinase,NEB,M0201S T4 DNA Polymerase,NEB,M0203S MyOne Streptavidin C1 beads,Invitrogen,65001 Klenow

(10) Conditional control of RNA-guided nucleic acid cleavage and gene editingNature CommunicationsDecember 1, 2020Shao-Ru Wang, Ling-Yu Wu, Hai-Yan Huang, Wei Xiong, Jian Liu, Lai Wei, Ping Yin, Tian Tian, Xiang ZhouNew England Biolabs (USA). Transcript Aid T7 High Yield Transcription kit (product# K0441) and Glycogen (product# R0561) were purchased from Thermo Fisher Scientific. Pyrobest™ DNA Polymerase and PrimeSTAR HS DNA Polymerase

(11) LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth controlNature cell biologyFebruary 1, 2020Gan W, Dai X, Dai X, Xie J, Yin S, Zhu J, Wang C, Liu Y, Guo J, Wang M, et al.protocol. The genomic DNA fragment containing the S606 site was amplified by PCR and digested by SfcI (NEB, R0561) to screen for the potential correct clones. Finally, successful knock-in mutant was verified by Sanger sequencing

(12) Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of TranscriptionCellJune 27, 2019Xiao R, Chen JY, Liang Z, Luo D, Chen G, Lu ZJ, Chen Y, Zhou B, Li H, Du X, et al.Proteins" Protease Inhibitor Cocktail,Roche,11873580001 Protein A/G magnetic beads,Thermo,88802 Glycogen,Thermo,R0561 BSA,NEB,B9000S tRNA,Thermo,15401011 Formaldehyde,Sigma-Aldrich,F8775-25ml Proteinase K,Thermo,EO0492 RNase A

(13) High-resolution, strand-specific R-loop mapping via S9.6-based DNA:RNA ImmunoPrecipitation and high-throughput sequencing.Nature ProtocolsJune 1, 2019Lionel A. Sanz, Frédéric Chédindinucleotide sodium salt (16 mM, Sigma-Aldrich, cat. No. N0632) Glycogen (ThermoFisher Scientific, cat. No. R0561) NEBNext End repair module (New England BioLabs, cat. No. E6050) Klenow fragment (3’ to 5’ exo-)

(14) Natural variation in C. elegans arsenic toxicity is explained by differences in branched chain amino acid metabolismeLifeApril 8, 2019Stefan Zdraljevic, Bennett William Fox, Christine Strand, Oishika Panda, Francisco J Tenjo, Shannon C Brady, Tim A Crombie, John G Doench, Frank C Schroeder, Erik C Andersenusing primers oECA1163 and oECA1165. The PCR products were digested using the SfcI restriction enzyme (R0561S, New England Biolabs, Ipswich, MA). Differential band patterns signified successfully edited strains

(15) Human proteins that interact with RNA/DNA hybridsGenome researchSeptember 1, 2018Wang IX, Grunseich C, Fox J, Burdick J, Zhu Z, Ravazian N, Hafner M, Cheung VG.(Thermo Fisher Scientific, #AM1334). The T7 promoter sequence in dsDNA was then removed by SfcI (NEB, # R0561S) digestion. The dsDNA and the transcribed ssRNA were dissolved in 10 mM Tris HCl at pH 8.0, 1 mM EDTA,

(16) Senataxin Mutation Reveals How R-Loops Promote Transcription by Blocking DNA Methylation at Gene PromotersMolecular cellFebruary 1, 2018Grunseich C, Wang IX, Watts JA, Burdick JT, Guber RD, Zhu Z, Bruzel A, Lanman T, Chen K, Schindler AB, et al.aliquot of the dsDNA, the ~20 bp T7 RNA polymerase promoter was removed by SfcI digestion (NEB, catalog # R0561S), and this dsDNA and the transcribed RNA were denatured, and then annealed in 10 mM Tris-HCl pH 8.0,

Be the First to Write a Review!

SfcI

SfcI from New England Biolabs

Sequence

New England Biolabs

Citations (16)

Reviews

SfcI from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map