Description
In many cases, sufficient material can be obtained through production from stable CHO cell pools instead of going all the way to produce a stable cell line. This approach is beneficial especially for time-sensitive projects, as recombinant antibodies and proteins become available within just a few weeks. During stable transfection, the DNA of interest is integrated into the genome of the host cell to allow steady production of the target protein. However, the location and number of DNA insertions vary from cell to cell. This creates a pool of cells that all express the same protein or antibody but with varying degrees of expression efficiency.
When developing a stable cell line, the resulting cell pool then serves as a starting point for single-cell cloning and various screening processes, in which the clone with the highest productivity is identified and selected as the basis for the stable cell line. By skipping the lengthy screening procedures and continuing with production directly from the cell pool, it is possible to reduce the costs and timeline of your project without compromising on the quality of the product.