Description
With Cayman Contract Services, your lipidomics study is in the hands of scientists who have several decades of collective experience in lipid synthesis, purification, and characterization. Cayman’s lipid analysis laboratories are equipped with state-of-the-art chromatography/mass spectrometry systems and data analysis software.
Cayman offers untargeted analysis to identify relative changes in samples across lipid classes (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, and sterol lipids)
Through untargeted approaches we analyze hundreds to thousands of lipid species from biological samples to create a detailed lipid profile. After using general liquid-liquid methods for extraction of most lipids within a sample, they are separated chromatographically using HPLC or UPLC in order to simultaneously detect individual molecular species and identify changes in these lipids between samples. Detection and identification of lipid species is achieved using high-resolution mass spectrometry (HRMS) and tandem mass spectrometry (MS/MS).
Identification and relative quantitation of candidate lipid species is facilitated by the use of data-processing software and publicly available databases. When molecular identification is provided based on the LC-MS/MS data available, Cayman adheres to the recommendations issued by the Lipid Maps® consortium and the Lipidomics Standards Initiative. Data is reported as tables of mass-to-charge ratios (m/z), with ion intensity peak areas normalized to internal standards, and they can be visualized using powerful plotting and graphing tools. Statistical analysis can also be applied to sort out significant changes within complex lipid profiles.