Description
From immunogen production to subcloning, we can help with all or a selection of stages. A detailed Statement of Work with clear transition points is written for every step of the process, allowing you to tailor the project in any way needed.
- Antigen production and purification
- Protein engineering to optimize immunogenicity of the antigen
- Immunization and serology
- Development of assays for hybridoma screening
- Hybridoma screening, production and purification
- Analysis of monoclonality using FluoroSpot
- Analysis of target affinity using Octet (Bio-layer interferometry)
- Hybridoma cell cloning by limited dilution
- Cell banking
We develop hybridomas from immunized mice or rats. If you already have an antigen – great! If you don’t have it – we’ll generate it for you. We have the possibility to increase immunogenicity using proprietary methods. All hybridomas are screened using ELISA, and promising hits can be further characterized using Affinity scout, epitope binning (Bio-layer interferometry), and FluoroSpot. Finally, selected hybridoma are subcloned to ensure monoclonality.