Description
RNA immunoprecipitation (RIP) is similar to chromatin immunoprecipitation (ChIP), but instead of DNA, RIP targets RNA-protein interaction. RIP precipitates a specific RNA binding protein (RBP) and associated RNAs which can be analyzed by real-time PCR, microarrays or sequencing. In addition to immunoprecipitation method, researchers sometimes fuse a tag at either the C-terminal or N-terminal of the protein of interest, and the tag antibody thus can be used as IP antibody.