Description
Creative Biostructure has a professional team of scientists who have been working with membrane proteins for years, and are actively establishing and improving related techniques within the membrane protein screening platform. Using the nanodiscs technology, our scientists have successfully incorporated various membrane protein targets of different topologies into the nanodisc bilayer to preserve MP structure and activity. By searching for an optimal molar stoichiometric ratio of lipid to scaffold protein as well as scaffold protein to MP (usually detergent-solubilized), the composition and homogeneity of the resultant assembly can be optimized to maximize the yield of MP incorporation. Membrane proteins prepared in nanodiscs are compatible with X-ray crystallography, EM and NMR studies.