SalI from New England Biolabs

Product Specs
ItemSalI
CompanyNew England Biolabs
Price $69.00 Inquire
Catalog NumberR0138S
Quantity2000 units
Concentration20-100 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G/TCGAC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopyNucleic Acids ResApril 28, 2026Sara De Bragança, Clara Aicart-Ramos, Ángel Rivera-Calzada, Raquel Arribas-Bosacoma, Mark S Dillingham, Oscar Llorca, Fernando Moreno-Herrerosite of the 1x poly-BbvCI plasmid. For this, the plasmid was digested with SalI (New England Biolabs, #R0138S), dephosphorylated using rSAP (New England Biolabs, #M0371S), and without further purification, ligated

(2) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Dempleby using miniprep spin columns (Qiagen, 27104). Linearization of pUC19 was achieved using SalI (NEB, R0138S) and BamHI (NEB, R3136S), and the resulting linear fragment was purified through agarose gel electrophoresis

(3) Genome-scale CRISPR screen identifies host factors associated with bovine parainfluenza virus 3 infectionVirulenceDecember 2, 2025Yuanchen Geng, Chuanwen Jiang, Huaiyi Zhang, Hao Yang, Yongchong Peng, Yingyu Chen, Changmin Hu, Hailong Liu, Sheng Li, Huanchun Chen, et al.pTRIP-MCS-3Flag-IRES-RFP vector was digested with SpeI and SalI (New England Biolabs, Ipswich, MA, USA, Cat. No.: R3133S, R0138S) at 37°C for 2 h, and the digestion products were verified by 1% agarose gel electrophoresis to confirm

(4) Recombination between fragile regions associated with chromosomal rearrangements in glioblastoma can be mediated by RAGsiScienceNovember 21, 2025Amita Paranjape, Susmita Kumari, Lipsa Rani Sahu, Amrita Mondal, Swapna Kunhiraman, Arun Sharma M, Namrata M. Nilavar, Bibha Choudhary, Sathees C. RaghavanStreptomycin,GIBCO,15140–122 Trypsin-EDTA,GIBCO,25200–072 Tetracyclin,Sigma,200-593-8 Puromycin,Sigma,200-387-8 Opti-MEM,GIBCO,31985070 DAPI,Sigma,D9542 Sephadex G-25,Sigma,S5772 NaCl,Sigma,S9625 γ-32P-ATP,BRIT,N/A BamH1,NEB,R0136 Sal1,NEB,R0138S T4 Polyneucleotide kinase,NEB,M0201S EcoR1,NEB,R0101 Glutathione-agarose resin,Sigma,G4510 Glutathione,Sigma,G4251 MOPS,Sigma,M1254 DMSO,Sigma,D4540 DTT,Sigma,D9779 Potassium

(5) Matrin-3 forms spherical and wormlike assemblies that are modulated by RNA binding and ALS/FTD-associated mutationsMolecular CellOctober 2, 2025Macy L. Sprunger, Min Kyung Shinn, Sabrina K. Talir, Ken Lee, Rohit V. Pappu, Meredith E. Jackrel,New England Biolabs,Cat# R0176S NotI-HF,New England Biolabs,Cat# R3189S SalI-HF,New England Biolabs,Cat# R0138S StyI-HF,New England Biolabs,Cat# R3500S XhoI,New England Biolabs,Cat# R0146S PfuUltra II Fusion HS DNA Polymerase

(6) Robust fluorescent labeling and tracking of endogenous non-repetitive genomic locibioRxivSeptember 1, 2025Alex Raterink, Rajarshi P. Ghosh, Linfeng Yang, Quanming Shi, Minh Khue Nguyen, Isaac B. Hilton, Jan T. Liphardt, Anna-Karin Gustavsson5’...-SalI-5xArray-BamHI...3’. Finally, the plasmids with the two 5x arrays were digested with BamHI-HF and SalI (NEB, R0138S), gel purified and ligated to create the 10x array. sgRNA design 5 kb regions adjacent to a gene of

(7) Recombinant pseudorabies virus expressing the consensus VP2 protein of porcine parvovirus 1 (PPV1) protects pigs against pseudorabies virus and PPV1Vet ResAugust 5, 2025Xiaoxiao Tian, Haojie Wang, Hao Song, Ziyi Wei, Xulong Zhu, Guoqing Liu, Mingxia Sun, Xinyi Huang, Meng Chen, Yandong Tang, et al.and right arm-F/R, and the left homologous arm was subsequently cloned and inserted into the Sal I (R0138S, NEB)-linearized pCAGGS plasmid to construct the pCAGGS-L-arm. The eGFP gene was amplified from the

(8) Single cell transcriptomes of zebrafish germline reveal progenitor types and feminization by Foxl2leLifeJune 11, 2025Chen-wei Hsu, Hao Hao, Ching-Hsin Yang, Yan-wei Wang, Ker-Chau Li, Bon-Chu ChungR0580), 3 U BglII (New England Biolabs, Cat. No. R0144S), and 6 U SalI (New England Biolabs, Cat. No. R0138S) were incubated with the following condition: 20 min in 37°C and 15 min in 16°C for three cycles, followed

(9) An improved model for prediction of de novo designed proteins with diverse geometriesbioRxivJune 6, 2025Benjamin Orr, Stephanie E. Crilly, Deniz Akpinaroglu, Eleanor Zhu, Michael J. Keiser, Tanja Kortemmeincreased protease resistance, was generated by restriction digest with SalI (New England Biolabs, #R0138S) overnight at 37°C. NdeI and BamHI (New England Biolabs, #R0111S, R3136S) were added to the reaction

(10) Unraveling the cohesin-chromatin interface: identifying protein interactions that modulate chromosome structure and functionEpigenetics ChromatinJune 2, 2025Natalie L. Rittenhouse, Riya Gohil, June E. Arricastres, Jill M. Dowenthe TRE of the parent piggyBac transposon vector by digestion with AgeI (NEB, R3552S) and SalI (NEB, R0138S) followed by Gibson Assembly (NEB, E2611S) to generate piggyBac vectors capable of doxycycline-inducible

(11) Psychedelic control of neuroimmune interactions governing fearNatureMay 1, 2025Chung EN, Lee J, Polonio CM, Choi J, Akl CF, Kilian M, Weiß WM, Gunner G, Ye M, Heo TH, et al.5′-TTTGAATTCTTAAGCGTAATCTGGAACATCGT-3′, followed by restriction digest using EcoRI-HF (NEB, R3101S) and SalI-HF (NEB, R0138S). Successfully cloned plasmids were sequenced by Plasmidsaurus. The plasmid was then digested with XbaI

(12) Dynamics of stress-induced c-fos expression in the rat prelimbic cortex: lessons from intronic and mature RNA and protein analysesNeurobiology of StressApril 22, 2025Patricia Molina, Xavier Belda, Sandra Beriain, Sara Serrano, Gentzane Compte, Raül Andero, Antonio Armariofrom Dr Lin (California Institute of Technology, Caltech, CA) and it was linearized with SalI (Ref. R0138S, New England BioLabs). The c-fos intronic probe targets specifically the first intron of the c-fos gene

(13) Rapid and robust generation of cardiomyocyte-specific crispants in zebrafish using the cardiodeleter systemCell Reports MethodsMarch 24, 2025Sean Keeley, Miriam Fernández-Lajarín, David Bergemann, Nicolette John, Lily Parrott, Brittany E. Andrea, Juan Manuel González-RosaRestriction enzyme,New England Biolabs,Cat# R0140L SalI Restriction enzyme,New England Biolabs,Cat# R0138L T4 DNA ligase,New England Biolabs,Cat# M0202L ,, Critical commercial assays,Critical commercial assays,Critical

(14) BT1549 coordinates the in vitro IL-10 inducing activity of Bacteroides thetaiotaomicronMicrobiology SpectrumMarch 4, 2025Morgan J. Engelhart, Orion D. Brock, Jessica M. Till, Robert W. P. Glowacki, Jason W. Cantwell, David J. Clarke, Darryl A. Wesener, Philip P. Ahernfragments flanking the gene of interest into the suicide pExchange-tdk vector using Sal1 (NEB, Cat. #R0138L) and Xbal (NEB, Cat. # R0145L) cut sites. pExchange-tdk contains a cloned copy of tdk (provides susceptibility

(15) Virus-induced vesicular acidification enhances HIV immune evasionbioRxivDecember 21, 2024Marianne E. Yaple-Maresh, Giselle G. Flores, Gretchen E. Zimmerman, Francisco Gomez-Rivera, Kathleen L. Collins,New England BioLabs,Cat. R3198 BssHII Enzyme,New England BioLabs,Cat. R0199S SalI Enzyne,New England BioLabs,Cat. R0138S NheI-HF Enzyme,New England BioLabs,Cat. R3131S T4 DNA Ligase,New England BioLabs,Cat. M0202 Corning

(16) AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virusJournal of VirologyNovember 19, 2024Anouk Lkharrazi, Kurt Tobler, Sara Marti, Anna Bratus-Neuenschwander, Bernd Vogt, Cornel Fraefelprotocol (39) and either digested extensively with HindIII, SalI, or mung bean nuclease (MBN) (R0104S, R0138S or M0250S, New England Biolabs, MA, USA) or left untreated. The DNA was separated on 0.8% agarose gels

(17) Activity of zebrafish THAP9 transposase and zebrafish P element-like transposonsbioRxivMarch 27, 2024Nitzan Kutnowski, George E. Ghanim, Yeon Lee, Donald C. Rio,New England Biolabs,R3131S NotI,New England Biolabs,R0189S PstI,New England Biolabs,R0140S SalI,New England Biolabs,R0138S Sau3A1,New England Biolabs,R0169S SpeI,New England Biolabs,R3133S XbaI,New England Biolabs,R0145S XhoI,New England Biolabs

(18) SAS: Split antibiotic selection for identifying chaperones that improve protein solubilityHeliyonMarch 15, 2024Emily McNutt, Na Ke, Alexandre Thurman, James B. Eaglesham, Mehmet Berkmenfolding factors were cloned into pAL0 that was digested with NcoI and SalI (New England Biolabs, cat#R0138S). Proteins native to E. coli were cloned from genomic DNA extracted from E. coli K-12 using the Monarch® Genomic DNA Purification Kit

(19) Pathogenic mycobacterium upregulates cholesterol 25-hydroxylase to promote granuloma development via foam cell formationiScienceMarch 15, 2024Shuang Zhou, Ding Zhang, Dan Li, Hankun Wang, Cairong Ding, Jingrui Song, Weifeng Huang, Xuan Xia, Ziwei Zhou, Shanshan Han, et al.stock,Sigma-Aldrich,Cat#O1391 PrimeSTAR® GXL Premix,Takara,Cat#R050A NotI,New England Biolabs,Cat#R0189S SalI,New England Biolabs,Cat#R0138S In-Fusion,Takara,Cat#638947 Lipomaster 2000 Transfection Reagent,Vazyme,Cat#TL201-01 Triton X-100,Sigma-Aldrich,Cat#T8787 Atorvastatin,MedChemExpress,Cat#HY-B0589 Rifampicin,MedChemExpress,Cat#HY-B0272 5-μm

(20) Generation of an enhancer-driven gene expression viral tool specific to dentate granule cell-types through direct hippocampal injectionFront NeurosciMarch 14, 2024Maria Letizia Potenza, Stefan Blankvoort, Miguel M. Carvalho, Joachim S. Grimstvedt, Valentina Di Maria, Kristian Moan, Rajeevkumar Raveendran Nair, Marcus S. Flatset, Qiangwei Zhang, Laurent F. Thomas, et al.(5′- GTGTACGTCGACTTGTATTGTAACATAGACTCTCACCT-3′) primer containing the restriction enzyme SalI (NEB™ R0138S) used for the amplification of the enhancer sequence of interest. Primers were dissolved in RNase free

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SalI

SalI from New England Biolabs

Sequence

New England Biolabs

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SalI from New England Biolabs

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