SalI-HF from New England Biolabs

Product Specs
ItemSalI-HF
CompanyNew England Biolabs
Price $67.00 Inquire
Catalog NumberR3138S
Concentration20,000 units/ml
Quantity2000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G^TCGAC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Neuron-derived neurotrophic factor-positive interneurons: a cellular target for anti-seizure therapiesEBioMedicineJuly 21, 2026Amy Richardson, Marion S. Mercier, Yoshiteru Shimoda, Robert T. Graham, Marco Leite, Alejandro Garcia, Maud Muller, Suraya A. Bond, Maria Grozdanova, Qimin Wu, et al.and the pAAV-hsyn-DIO-hM3D(Gq)-mCherry plasmid were digested with MluI (NEB; R3198S) and SalI (NEB; R3138S). The DNA was then purified (PureLink PCR Micro kit; K310050) and the fragment ligated into the hM3D(Gq)

(2) Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidCThe EMBO JournalJune 26, 2026Max Busch, Cristian Rosales-Hernandez, Michael Kamel, Yulia Schaumkessel, Eli O van der Sluis, Otto Berninghausen, Thomas Becker, Roland Beckmann, Alexej Kedrovother reagents" Phusion High-Fidelity DNA Polymerase,New England Biolabs, (NEB),M0530L, SalI,NEB,R3138S NheI,NEB,R3131S HindIII,NEB,R3104S T4DNA Ligase,NEB,M0202S PNK,NEB,M0201S DpnI,NEB,R0176S Kanamycin,Sigma-Aldrich,60615 Ampicillin,Sigma-Aldrich,A0166 Arabinose,Roth,5118.2 Glucose,Fisher

(3) Atg23 interacts with both the N- and C-termini of Atg9 via a hydrophobic binding pocketJournal of Biological ChemistryJune 12, 2026Zhanibek Bekkhozhin, Kelsie A. Leary, Michael J. RagusaGibson Assembly master mix (NEB E2611S), T4 ligase (NEB M0202S); BamHI-HF, SalI-HF, NcoI-HF, NotI-HF, DpnI (NEB R3136L, R3138S, R3193S, R3189S, R0176S); 2× GoTaq master mix (Promega M7122), NEB5 alpha (NEB C2987H), Rosetta2 (DE3)

(4) Mutual potentiation of IPA1 and OsNPR1 enhances rice immunityPlant CellMay 20, 2026Zhen Wang, Mingming Liu, Rujuan Yan, Xiaohan Zhang, Xiaoguang Song, Guosheng Xiong, Jiayang Li, Zuhua He, Dong-Lei YangData Set S5). The OsCUL3a DNA fragment was cloned into the pCAMBIA1305-3xHA vector digested with SalI (R3138S, NEB) and HindIII (R3104S, NEB) through homologous recombination using a ClonExpress II One Step Cloning Kit (C112

(5) STREMI: a dual-function upstream ORF-encoded regulator of mitochondrial cristae architectureEMBO ReportsMay 2, 2026Ruiyang Guo, Yabo Guo, Runguo Shu, Jiajia Qian, Jiawei Wang, Ruobing Li, Ti Qin, Ziyi Wang, Hongtao Tian, Mengchen Wu, et al.enzyme,NEB,Cat#R3136S EcoRI-HF restriction enzyme,NEB,Cat#R3101S SalI-HF restriction enzyme,NEB,Cat#R3138S NheI-HF restriction enzyme,NEB,Cat#R3131S NdeI restriction enzyme,NEB,Cat#R0111V DpnI restriction

(6) Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20International journal of molecular sciencesApril 29, 2026Corsi A, Valentino A, Bruno MG, Menichetti G, Belpinati F, Pereira MP, Valenti MT, Ruggiero A, Trabetti E, Bombieri C, et al.enzymatically cut using XbaI (cat R0145S, New England Biolabs, Ipswich, MA, USA) and SalI High Fidelity® (cat R3138S, New England Biolabs, Ipswich, MA, USA) enzymes, according to the manufacturer’s instructions. The digested

(7) A proximity-labeling map of PI5P4K phosphoinositide kinases interaction networksJournal of Biological ChemistryApril 25, 2026Alicia Llorente, Gurpreet K. Arora, Ryan M. Loughran, Rabi Murad, Svetlana Maurya, Sophia Crabtree, Benji Portillo, Pratima Chapagain, Hundaol Huluka, Scott Eron, et al.plasmid #80900, https://www.addgene.org/80900/) using XhoI and SalI restriction enzymes (R0146S and R3138S, New England Biolabs) and the same cloning workflow described above. For lentiviral production, human

(8) Germline-targeting HIV immunogen induces cross-neutralizing antibodies in outbred macaquesImmunityApril 14, 2026Nitesh Mishra, Bo Liang, Ryan S. Roark, Amrit Raj Ghosh, Sean Callaghan, Wen-Hsin Lee, Xuduo Li, Anh L. Vo, Gabriel Avillion, Rohan Roy Chowdhury, et al.,New England Biolabs,Cat# R3131S BsiWI-HF,New England Biolabs,Cat# R3553S SalI-HF,New England Biolabs,Cat# R3138S SPRIselect,Beckman Coulter Genomics,Cat# B23318 Magnesium Chloride Hexahydrate,Fisher bioreagents,Cat#

(9) Exonic enhancers are a widespread class of dual-function regulatory elementsNature CommunicationsApril 2, 2026Jean-Christophe Mouren, Magali Torres, Antoinette van Ouwerkerk, Iris Manosalva, Frederic Gallardo, Salvatore Spicuglia, Benoit Ballesterfor 25 min. The STARR-seq vector was digested with AgeI-HF, SalI-HF, and NotI-HF (NEB, cat. #R3552S, #R3138S, #R3189S), loaded onto a 1.5% agarose gel at 100 V for 45 min, and the desired band was extracted using

(10) Substrate-interacting pore loops of two ATPase subunits determine the degradation efficiency of the 26S proteasomeNature CommunicationsMarch 24, 2026Erika López-Alfonzo, Ayush Saurabh, Sahar Zarafshan, Connor Arkinson, Christine L. Gee, Hao-Hsuan Hsieh, Steve Pressé, Andreas Martinconstructed by subcloning using the respective restriction enzymes (NEB; PstI (Cat# R3140S), SalI (Cat# R3138S), NotI (Cat# R3189S), BlpI (Cat# R0585S), FseI (Cat# R0588S), or PacI (Cat# R0547S)) and T7 DNA ligase

(11) AP-1 mediates cellular adaptation and memory formationNature CommunicationsMarch 20, 2026Jingxin Li, Pavithran T. Ravindran, Aoife O’Farrell, Gianna T. Busch, Ryan H. Boe, Zijian Niu, Sean Woo, Margaret C. Dunagin, Naveen Jain, Yogesh Goyal, et al.Both the lentiviral plasmid and the PCR amplicon were double digested with ClaI (R0197S) and SalI (R3138S) and processed with a PCR & DNA cleanup kit (NEB T1030S). To generate the EGFP reporter, we PCR amplified

(12) RT-qPCR and split-luciferase assays enable batch standardization and analysis of engineered virus-like particle transductionMolecular Therapy AdvancesMarch 12, 2026Lucia Nicosia, Joss B. Murray, Emma Collins, Lisa Lonetti, Patrick T. Harrison, Martina F. ScallanPCR product (insert) and pAAVS1-SA-2A-Puro (vector) were digested at 37°C for 4 h with SalI-HF (NEB, Cat#R3138S). After digestion, pAAVS1-SA-2A-Puro was dephosphorylated with Antarctic phosphatase, (NEB, Cat#M0289)

(13) Plakoglobin transmits tension across VE-cadherin for vascular leak formation and leukocyte diapedesisThe EMBO JournalMarch 11, 2026Neha Uttekar, Annette Artz, Vallari Ghanekar, Pragya Kaul, Jessica Heinrichs, Rebekka I Stegmeyer, Gizem Gülevin Takir, Astrid F Nottebaum, Dietmar VestweberLipofectamine RNAiMAX Transfection reagent,Invitrogen,13778150 Tamoxifen,Sigma-Aldrich,T5648-5G SalI,New England Biolabs,R3138S MluI,New England Biolabs,R3198L Il-1β,BIOMOL GmbH,50441 Evans blue solution,Sigma-Aldrich,E2129 Histamine,Sigma-Aldrich,H7250-10MG Formamide,Sigma-Aldrich,11814320001 TNF-α,Sigma-Aldrich,SRP3177-50UG CellTracker

(14) CD8 + T cells are primed by cDC1 and exacerbate tau-mediated neurodegenerationbioRxivMarch 1, 2026Hao Hu, Peter Bor-Chian Lin, Carisa Zeng, Prabal Sharma, Yongyi Li, Hong Jiang, Jonathan Nulman, Ray A. Ohara, Tong Wu, Shasha Li, et al.CTG C The amplified DNA was purified and digested by restriction endonuclease enzymes SalI (NEB, Cat# R3138S) and PmeI (NEB, Cat# R0560S) per instruction (https://nebcloner.neb.com/#!/redigest). The rAAV8 plasmid

(15) Protocol for targeted gene manipulation and thermogenic evaluation in mouse brown adipocytesSTAR ProtocolsMarch 1, 2026Jakub Bunk, Drishti Soni, Matthias Calderon, Bozena Samborska, Lawrence Kazak,New England Biolabs (NEB),N0447S HindIII-HF,New England Biolabs (NEB),R3104S SalI-HF,New England Biolabs (NEB),R3138S 10× rCutSmart Buffer,New England Biolabs (NEB),B6004S "Nuclease-free H2O –Dnase, RNase, Protease Free",Wisent

(16) Functional effects of EpCAM N-glycosylation in MDA-MB-468 breast cancer cellsScientific ReportsFebruary 20, 2026Nicole M. Jenkinson, Harsh Oza, Kevin J. Yarema, Kristine Glunderestriction enzyme cloning with XbaI (New England Biolabs #R0145S) and SalI-HF (New England Biolabs #R3138S) using NEB rCutSmart Buffer (New England Biolabs #B6004S). Fragments were isolated by agarose (VWR #490001-580)

(17) Histone variant H2A.Z mutant suppresses the senescence-associated secretory phenotypebioRxivFebruary 20, 2026Zong Ming Chua, Hiroshi Tanaka, Adrianna Abele, Adarsh Rajesh, Marcos Garcia Teneche, Xue Lei, Karl Miller, Andrew Davis, Carolina Cano Macip, Laurence Haddadin, et al.sequences into plasmid was carried out using the restriction endonucleases BamHI (R3136S) and Sall (R3138S) for digestion of both insert and plasmid sequences while T4 ligase (M0202L) was used for the subsequent

(18) Epromoters bind key stress-related transcription factors to regulate clusters of stress response genesThe EMBO JournalJanuary 3, 2026Juliette Malfait, Jing Wan, Himanshu Narayan Singh, Charbel Souaid, Gaëlle Farah, Junhua Su, Magali Torres, Iris Manosalva, Nathalie Sakakini, Cyril Esnault, et al.,Thermo Fisher Scientific,10378016 Dual-Luciferase kit,Promega,E1980 BamHI-HF,New England Biolabs,R3136S SalI-HF,New England Biolabs,R3138S BglII,New England Biolabs,R0144S HindIII,New England Biolabs,R0104S Alt-R CRISPR-Cas9 System,Integrated DNA Technologies

(19) Atg23 Interacts With Both the N- and C-termini of Atg9 Via a Hydrophobic Binding PocketbioRxivDecember 22, 2025Zhanibek Bekkhozhin, Kelsie A Leary, Michael J RagusaGibson Assembly master mix (NEB E2611S), T4 ligase (NEB M0202S); BamHI-HF, SalI-HF, NcoI-HF, NotI-HF, DpnI (NEB R3136L, R3138S, R3193S, R3189S, R0176S); 2× GoTaq master mix (Promega M7122), NEB5 alpha (NEB C2987H), Rosetta2 (DE3)

(20) Evaluating the Antifungal Potential of Autophagy-Related Protein 4 (ATG4) Inhibitors against Human Fungal PathogensJ Microbiol BiotechnolDecember 15, 2025Seungmee Jung, Jongchan Woo, Hyunjin Cha, Seung-Heon Lee, Sagar Dahal, Yong-Sun Bahn, Eunsook Park(G3XZT6), digested with BamHI-HF (New England Biolabs, R3136S, USA) and SalI-HF (New England Biolabs, R3138S), were cloned into the corresponding site of the pET28-Citrine-BcAtg8-ShR plasmid [28], replacing BcAtg8

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4/11/2018Diagnostic Restriction Mapping Of Plasmid Constructs With SalI-HF Triveni MahendrappaI routinely construct several different types of plasmids for cloning and expression purposes. I have constructed plasmid of a size 6134 bp. After transforming it into suitable E. coli cells, colonies... Read Review

SalI-HF

SalI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (120)

Reviews

SalI-HF from New England Biolabs

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