RNasin Plus RNase Inhibitor from Promega

Product Specs
ItemRNasin Plus RNase Inhibitor
CompanyPromega
Price Get Pricing Get Quote for this Product
Catalog NumberN2615
Quantity10,000u
Concentration40 U/µL
SourceRecombinant E. Coli

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Role of affinity in plasma cell development in the germinal center light zoneJournal of Experimental MedicineJanuary 1, 2024Mohamed A. ElTanbouly, Victor Ramos, Andrew J. MacLean, Spencer T. Chen, Maximilian Loewe, Sandra Steinbach, Tarek Ben Tanfous, Brianna Johnson, Melissa Cipolla, Anna Gazumyan, et al.tergitol (0.5% of NP-40 70% in H2O, Sigma-Aldrich, NP40S), and RNase inhibitor (0.6 U/μl, Promega, N2615) in nuclease-free water (Qiagen) and then incubated at 65°C for 3 min. cDNA was subsequently synthesized

(2) Novel N-methylsulfonyl-indole derivatives: biological activity and COX-2/5-LOX inhibitory effect with improved gastro protective profile and reduced cardio vascular risksJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2023John N. Philoppes, Mohamed A. Abdelgawad, Mohammed A. S. Abourehab, Mohamed Sebak, Mostafa A. Darwish, Phoebe F. LamieInc.). For the experiments, CL Buffer included MgCl2 (M1028; Sigma) or RNasin Plus RNase Inhibitor (N2615; Promega). Before use, both Bio-Rad SPR and CL buffer were equilibrated to room temperature. Cells were

(3) Potent latency reversal by Tat RNA-containing nanoparticle enables multi-omic analysis of the HIV-1 reservoirNat CommunDecember 18, 2023Marion Pardons, Basiel Cole, Laurens Lambrechts, Willem van Snippenberg, Sofie Rutsaert, Ytse Noppe, Nele De Langhe, Annemieke Dhondt, Jerel Vega, Filmon Eyassu, et al.p24 28B7-APC) (45 min, RT) in PBS/1% BSA (ThermoFisher, #AM2616)/RNAse inhibitor 0.4 U/μL (Promega, #N2615)/DTT 1 mM. All washing steps following methanol permeabilization were done in PBS/0.04% BSA. In all

(4) Evolution of neuronal cell classes and types in the vertebrate retinaNatureDecember 13, 2023Joshua Hahn, Aboozar Monavarfeshani, Mu Qiao, Allison H. Kao, Yvonne Kölsch, Ayush Kumar, Vincent P. Kunze, Ashley M. Rasys, Rose Richardson, Joseph B. Wekselblatt, et al.mM CaCl2, 8 mM MgCl2, 15 mM NaCl, 0.1 U μl−1 RNAse inhibitor (Promega RNasin Ribonuclease Inhibitor N2615), and 0.02 U μl−1 DNAse (D4527, Sigma Aldrich). The homogenized tissue was passed through a 40-µm cell

(5) Sperm chromatin accessibility's involvement in the intergenerational effects of stress hormone receptor activationTransl PsychiatryDecember 8, 2023Vincent Fischer, Miriam Kretschmer, Pierre-Luc Germain, Jasmine Kaur, Sergio Mompart-Barrenechea, Pawel Pelczar, David Schürmann, Primo Schär, Katharina GappM1705) and Oligo(dt) 15 primers (Promega, C1101) containing RNasin® Plus RNase inhibitor (Promega, N2615) according to the manufacturer’s recommendations. The resulting cDNA samples were diluted 1:4 and 2

(6) Temporal and spatial analysis of astrocytes following stroke identifies novel drivers of reactivitybioRxivNovember 28, 2023Rachel D Kim, Anne E Marchildon, Paul W Frazel, Philip Hasel, Amy X Guo, Shane A Liddelowbuffer containing of 1% Kollidon VA64, 1% Triton X-100 (Sigma-Aldrich T8787), 1:100 RNasin Plus (Promega N2615), and 1:200 Alexa Fluor 488 anti-GFP antibody (BioLegend 338007) in dissociation buffer. Samples were

(7) Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycleNature CommunicationsNovember 27, 2023Shuyao Hu, Yufeng Zhang, Qianqian Yi, Cuiwei Yang, Yanfen Liu, Yun Baiμg/mL Heparin, 0.5% NP-40, 1:5000 antifoam B, 0.5 mM DTT, 1 U/μL RNasin® Plus RNase Inhibitor (Promega, N2615), and protease inhibitor (APExBIO, K1010; added immediately before use)) on ice and lysed by passing

(8) Neural adaption in midbrain GABAergic cells contributes to high-fat diet–induced obesityScience AdvancesNovember 3, 2023Xiaomeng Wang, Xiaotong Wu, Hao Wu, Hanyang Xiao, Sijia Hao, Bingwei Wang, Chen Li, Katherin Bleymehl, Stefan G. Kauschke, Volker Mack, et al.Sigma-Aldrich) supplemented with protease inhibitor (5892791001; Roche) and ribonuclease inhibitor (N2615, Promega and AM2696, Life Technologies). Chromium 10x Genomics library and sequencing Single-nuclei

(9) Remyelination protects neurons from DLK-mediated neurodegenerationbioRxivOctober 18, 2023Greg J Duncan, Samantha D Ingram, Katie Emberley, Jo Hill, Christian Cordano, Ahmed Abdelhak, Michael McCane, Nora Jabassini, Kirtana Ananth, Skylar J. Ferrara, et al.protein inhibitor cocktail (cOMPLETE Mini lacking EDTA, 11873580001, Roche) along with 15uL of RNAsin (N2615, Promega) per 10 mL NIB. When optic nerves were removed from the freezer, they were immediately placed

(10) Cell type-specific gene expression dynamics during human brain maturationbioRxivOctober 6, 2023Christina Steyn, Ruvimbo Mishi, Stephanie Fillmore, Matthijs B. Verhoog, Jessica More, Ursula K. Rohlwink, Roger Melvill, James Butler, Johannes M. N. Enslin, Muazzam Jacobs, et al.serum albumin [BSA, Sigma-Aldrich, A2153–10G, US], and 0.2 U/μl RNAsin Plus RNase inhibitor [Promega, N2615, US]). Resuspended nuclei were passed through a 40 μm filter and centrifuged at 900 × g for 10 minutes

(11) Binding of the nuclear ribonucleoprotein family member FUS to RNA prevents R-loop RNA:DNA hybrid structuresThe Journal of biological chemistryOctober 1, 2023Thompson VF, Wieland DR, Mendoza-Leon V, Janis HI, Lay MA, Harrell LM, Schwartz JC.Tris-HCl pH 8, 5 mM DTT, 10 mM MgCl2) with 2 mM NTP’s (NEB, N0450S) and 40 U/μl RNasin Plus (Promega, N2615). T7 (P266L) polymerase (20) was added to linearized plasmid DNA (25 ng/μl) at 37 °C, incubated for

(12) Rapid isolation of intact retinal astrocytes: a novel approachActa neuropathologica communicationsSeptember 25, 2023Cullen PF, Mazumder AG, Sun D, Flanagan JG.concentration "DTT (VWR # 97,061–340)",1 M "Protease Inhibitor (VWR # 97,063–970)",‘100x’ RNasin (Promega # N2615),40 u/µl "Cycloheximide (Thermo Scientific # 357,420,050)",5 mg/ml Heparin (Thermo Scientific # BP2524250),100

(13) Therapeutic blockade of ER stress and inflammation prevents NASH and progression to HCCScience AdvancesSeptember 13, 2023Ebru Boslem, Saskia Reibe, Rodrigo Carlessi, Benoit Smeuninx, Surafel Tegegne, Casey L. Egan, Emma McLennan, Lauren V. Terry, Max Nobis, Andre Mu, et al.supplemented with 1% bovine serum albumin (BSA) and RNasin Plus ribonuclease inhibitor (0.2 U/μl; Promega, N2615)]. Following another round of centrifugation, nuclei were resuspended in 700 μl of nuclei wash buffer

(14) Drugs of abuse hijack a mesolimbic pathway that processes homeostatic needbioRxivSeptember 10, 2023Bowen Tan, Caleb J. Browne, Tobias Nöbauer, Alipasha Vaziri, Jeffrey M. Friedman, Eric J. Nestlerbuffer, 1 µl DTT, 25 µl 20 U/µl SupeRasine (Thermo Cat# AM2696), 12.5 µl 40 U/ µl RNasin (Promega Cat# N2615), 10 µl protease and phosphatase inhibitor cocktail (100X; Thermo Cat# 78442), 40 µl 1 mg/ml actinomycin D

(15) Memory B cell development elicited by mRNA booster vaccinations in the elderlyJournal of Experimental MedicineSeptember 4, 2023Zijun Wang, Frauke Muecksch, Raphael Raspe, Frederik Johannsen, Martina Turroja, Marie Canis, Mohamed A. ElTanbouly, Gabriela S. Silva Santos, Brianna Johnson, Viren A. Baharani, et al.μl of lysis buffer (0.5 × PBS, 10 mM dithiothreitol, 3,000 units/ml RNasin Ribonuclease Inhibitors [N2615; Promega]) per well using a FACS Aria III and FACSDiva software (Becton Dickinson) for acquisition and

(16) CD31 defines a subpopulation of human adipose-derived regenerative cells with potent angiogenic effectsScientific reportsSeptember 1, 2023Dhumale P, Nielsen JV, Hansen ACS, Burton M, Beck HC, Jørgensen MG, Toyserkani NM, Haahr MK, Hansen ST, Lund L, et al.were resuspended in 500 µl PBS with 1% BSA and 1 U/µl RNAsin PLUS RNase Inhibitor (Promega, Cat.no. N2615) and passed through a 40 µm Flowmi® Cell strainer (VWR, cat.no. 734-5950) to create a single-cell suspension.

(17) Epithelial Ca2+ waves triggered by enteric neurons heal the gutbioRxivAugust 20, 2023Afroditi Petsakou, Yifang Liu, Ying Liu, Aram Comjean, Yanhui Hu, Norbert Perrimonbuffer [250mM Sucrose, 10mM Tris pH8, 25mM KCl, 5mM MgCl, 0.1% Triton-X, 0.5% RNasin Plus (Promega, N2615), 50x protease inhibitor (Promega G6521), 0.1mM DTT]. Using 1ml dounce (Wheaton 357538), nuclei were

(18) Early chromosome condensation by XIST builds A-repeat RNA density that facilitates gene silencingCell reportsJuly 25, 2023Valledor M, Byron M, Dumas B, Carone DM, Hall LL, Lawrence JB.Sigma,Cat# 11093088910 Formaldehyde,Millipore Sigma,Cat# 47671 RNasin Plus RNase inhibitor,Promega,Cat# N2615 Triton X-100,Millipore Sigma,Cat# 11332481001 Paraformaldehyde,Ted Pella,Cat# 18505 PBAE (poly(β-amino

(19) In vitro selection of macrocyclic peptide inhibitors containing cyclic γ2,4-amino acids targeting the SARS-CoV-2 main proteaseNature ChemistryJuly 1, 2023Takashi Miura, Tika R. Malla, C. David Owen, Anthony Tumber, Lennart Brewitz, Michael A. McDonough, Eidarus Salah, Naohiro Terasaka, Takayuki Katoh, Petra Lukacik, et al.1 mM spermidine, 0.01% Triton X-100, 3.75 mM NTP mix, 0.04 U μl–1 RNasin RNase inhibitor (Promega, N2615) and 120 nM T7 RNA polymerase (37 °C, 16 h). For transcription of tRNAs, 5 mM GMP was added to the above

(20) Interaction between host G3BP and viral nucleocapsid protein regulates SARS-CoV-2 replicationbioRxiv : the preprint server for biologyJune 30, 2023Yang Z, Johnson BA, Meliopoulos VA, Ju X, Zhang P, Hughes MP, Wu J, Koreski KP, Chang TC, Wu G, et al.U/mL RNase I for 3 min at 37°C. The reaction was stopped by the addition of 0.4 U/μL RNaseIn (Promega; N2615). Lysates were then centrifuged at 21,000 x g for 10 min at 4°C. The supernatant fraction was incubated

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