pRS shRNA Vector from OriGene Technologies

Product Specs
ItempRS shRNA Vector
CompanyOriGene Technologies
Price $670.00Supplier Page View Company Product Page
Catalog NumberTR20003
Quantity5 µg
MCSBamHI, HindIII
PromoterU6
ResistancePuromycin
Selectable MarkerAmpicillin
Origin of ReplicationpBS
TypeshRNA Vectors

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OriGene Technologies

9620 Medical Center Drive # 200

Rockville, Maryland 20850

United States

Phone: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254

Company Profile

Website: http://www.origene.com

(1) OPRK1 drives SLC9A3R1 progression to neuroendocrine prostate cancerCell Death & DiseaseDecember 7, 2025Linghui Liang, Zhiyi Shen, Yuwei Zhang, Yifei Cheng, Bing Yao, Ninghan Feng, Ruizhe Zhao(sc-45597-SH). The shSLC9A3R1 sequence (5’—3’) is subcloned into the pRS shRNA cloning plasmid (Origene, TR20003). By inserting the kpn I/XhoI fragment containing the OPRK1 promoter (-1987bp ~ +1991bp) cloned from

(2) EP300-mediated H3 acetylation elevates MTHFD2 expression to reduce mitochondrial dysfunction in lipopolysaccharide-induced tubular epithelial cellsRenal FailureDecember 31, 2024Weike HuGenePhrama (Suzhou, China). pCMV6-Entry vectors (NC; PS100001, OriGene, USA) and pRS vectors (shNC; TR20003, OriGene, USA) were used as the negative control for the overexpression plasmids and shRNA, respectively.

(3) Cpne7 deficiency induces cellular senescence and premature aging of dental pulpAging cellMarch 1, 2024Lee YS, Park YH, Hwang G, Seo H, Ki SH, Bai S, Son C, Roh SM, Park SJ, Lee DS, et al.Recombinant CPNE7 (rCPNE7; Oriegene, NP_705900) were purchased from Origene (Rockville, MD). Control shRNA (TR20003) and shRNA targeting Cpne7 (TR305242) were purchased from Origene (Rockville, MD). The cells were transiently

(4) Piezo1 activation facilitates ovarian cancer metastasis via Hippo/YAP signaling axisChannels (Austin)December 31, 2022Yanjie Xiong, Liru Dong, Yun Bai, Hui Tang, Shuang Li, Dan Luo, Fang Liu, Jie Bai, Shikun Yang, Xudong Songplasmid containing shRNA specific to Piezo1 (Origene, Cat # TF313084) or the Veh-shRNA (Origene, cat # TR20003) was transfected to A-1847 cells cultured in 6-well plates in the serum-free medium using lipofectamine

(5) Calnexin Is Involved in Forskolin-Induced Syncytialization in Cytotrophoblast Model BeWo CellsBiomoleculesJuly 28, 2022Matsukawa H, Ikezaki M, Nishioka K, Iwahashi N, Fujimoto M, Nishitsuji K, Ihara Y, Ino K.shRNA vectors containing short hairpin RNA for CNX (CNX-shRNA, #TR314210) and non-specific shRNA (#TR20003), and the puromycin-resistant marker from OriGene Technologies (Rockville, MD, USA). We transfected

(6) Phospholipase C-ε regulates bladder cancer cells via ATM/EXO1American journal of cancer researchJanuary 1, 2020Fan J, Zhao Y, Yuan H, Yang J, Li T, He Z, Wu X, Luo C.

(7) Sirtuin 3 silencing impairs mitochondrial biogenesis and metabolism in colon cancer cellsAmerican journal of physiology. Cell physiologyAugust 1, 2019Torrens-Mas M, Hernández-López R, Pons DG, Roca P, Oliver J, Sastre-Serra J.

(8) CCN1 induces apoptosis in esophageal adenocarcinoma through p53-dependent downregulation of survivinJournal of cellular biochemistryFebruary 1, 2019Dang T, Modak C, Meng X, Wu J, Narvaez R, Chai J.

(9) Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomesEMBO reportsDecember 1, 2018Shakya S, Sharma P, Bhatt AM, Jani RA, Delevoye C, Setty SR.

(10) Dlk1-Dio3 locus-derived lncRNAs perpetuate postmitotic motor neuron cell fate and subtype identityeLifeOctober 12, 2018Yen YP, Hsieh WF, Tsai YY, Lu YL, Liau ES, Hsu HC, Chen YC, Liu TC, Chang M, Li J, et al.shRNA The Meg3 HuSH-29 shRNA plasmids (Origene, cat. No. TG501330) and non-effective scrambled sequence (TR20003) were used to create stable knockdown lines of Meg3 within the Hb9::GFP ESCs. We used two different

(11) Regulation of vascular signalling by nuclear Sprouty2 in fetal lung epithelial cells: Implications for co-ordinated airway and vascular branching in lung developmentComparative biochemistry and physiology. Part B, Biochemistry & molecular biologyOctober 1, 2018Walker DJ, Land SC.were generated at positions 218 and 221 to assess Spry2-DNA interactions. Non-genome target control (TR20003) and shRNA vectors targeting human Spry2 in pRS were originally purchased from Origene Technologies,

(12) Rab4A organizes endosomal domains for sorting cargo to lysosome-related organellesJournal of cell scienceSeptember 20, 2018Nag S, Rani S, Mahanty S, Bissig C, Arora P, Azevedo C, Saiardi A, van der Sluijs P, Delevoye C, van Niel G, et al.

(13) EBP50 suppresses the proliferation of MCF-7 human breast cancer cells via promoting Beclin-1/p62-mediated lysosomal degradation of c-MycActa pharmacologica SinicaAugust 1, 2018Liu H, Zhao WL, Wang JP, Xin BM, Shao RG.(5’-CACCGACCAGAAACGCAGCAGCAAACTTCAAGAGAGTTTGCTGCTGCGTTTCTGGTTTTTTTG-3’) was subcloned into the pRS shRNA cloning plasmid (Origene, TR20003). A control shRNA oligonucleotide that did not match any known human coding cDNA was used as a control.

(14) Hypoxia-induced HMGB1 expression of HCC promotes tumor invasiveness and metastasis via regulating macrophage-derived IL-6Experimental cell researchJune 1, 2018Jiang J, Wang GZ, Wang Y, Huang HZ, Li WT, Qu XD.

(15) Annexin A2 Mediates the Localization of Measles Virus Matrix Protein at the Plasma MembraneJournal of virologyMay 15, 2018Koga R, Kubota M, Hashiguchi T, Yanagi Y, Ohno S.

(16) A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancerThe Journal of clinical investigationMarch 1, 2017Berlato C, Khan MN, Schioppa T, Thompson R, Maniati E, Montfort A, Jangani M, Canosa M, Kulbe H, Hagemann UB, et al.(TR314127) and mouse (TR500386) CCR4. A nonspecific shRNA sequence (shGFP, TR30003) and empty pRS vector (TR20003) served as controls. Phoenix packaging cell line was transfected overnight with LipofectAMINE 2000 (Invitrogen)

(17) Nm23H1 mediates tumor invasion in esophageal squamous cell carcinoma by regulation of CLDN1 through the AKT signalingOncogenesisJuly 4, 2016Kuo KT, Chen CL, Chou TY, Yeh CT, Lee WH, Wang LS.mm), HEPES (10 mm) and β-mercaptoethanol (50 μm). Cells were stably transfected with the pRS vector (# TR20003; cell line Vec-shRNA; Origene, Rockville, MD, USA), pRS-short hairpin (sh) GFP (Origene # TR30003; encoding

(18) Peroxiredoxin 6 (Prdx6) supports NADPH oxidase1 (Nox1)-based superoxide generation and cell migrationFree radical biology & medicineJuly 1, 2016Kwon J, Wang A, Burke DJ, Boudreau HE, Lekstrom KJ, Korzeniowska A, Sugamata R, Kim YS, Yi L, Ersoy I, et al.University) and Mouse monoclonal Ab against p22phox (no. 449) were described previously [9]. pRS vector (#TR20003), GFP-specific shRNA vector (#TR30003), and four human Prx6-targeted pRS shRNA expression vectors (#TR310191)

(19) Mic60/mitofilin overexpression alters mitochondrial dynamics and attenuates vulnerability of dopaminergic cells to dopamine and rotenoneNeurobiology of diseaseJuly 1, 2016Van Laar VS, Berman SB, Hastings TG.from OriGene Technologies (cat#TR312153), which included the empty vector pRS plasmid negative control TR20003 (EV-shRNA), the non-functional GFP-targeted shRNA construct negative control TR30003 (GFP-shRNA), and

(20) Actin-Modulating Protein Cofilin Is Involved in the Formation of Measles Virus Ribonucleoprotein Complex at the Perinuclear RegionJournal of virologyOctober 1, 2015Koga R, Sugita Y, Noda T, Yanagi Y, Ohno S.

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pRS shRNA Vector

pRS shRNA Vector from OriGene Technologies

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OriGene Technologies

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pRS shRNA Vector from OriGene Technologies

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