PCMVMIR MicroRNA Expression Vector from OriGene Technologies

Product Specs
ItemPCMVMIR MicroRNA Expression Vector
CompanyOriGene Technologies
Price $670.00Supplier Page View Company Product Page
Catalog NumberPCMVMIR
Quantity10 µg
TypeMicroRNA Vector
Conjugate/Tag/Label (standardized)GFP tag
Nucleic Acid TypemiRNA Clones
Vector Type (Standardized)Retroviral

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OriGene Technologies

9620 Medical Center Drive # 200

Rockville, Maryland 20850

United States

Phone: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254

Company Profile

Website: http://www.origene.com

(1) MicroRNA‑24 alleviates colorectal cancer progression via a rs28382740 single nucleotide polymorphism in the long noncoding region of X‑linked inhibitor of apoptosis proteinOncology LettersOctober 4, 2024Yu-Feng Tian, Chi-Jung Huang, Chih-Yi Liu, Shung-Haur Yang, Chih-Sheng Hung, Kai-Yuan Lin, Ching-Long Lai, Chun-Chao Chang(pMIR-REPORT-A and pMIR-REPORT-G) in HCT116 cells were assessed by cotransfection of pCMV-MIR vectors (cat. no. PCMVMIR; OriGene Technologies, Inc.) with miR-24-1 precursor (cat. no. SC400296; OriGene Technologies, Inc.)

(2) Identification of miR-6794-3p as a suppressor in pancreatic cancer metastasisInternational Journal of Biological SciencesSeptember 30, 2024Ha Gyeong Kim, Yunmi Cho, Jae-Seon Lee, Eun-Taex Oh, Heon Joo Parkexpressing MAST1 (#VB900132-7646rqa) was purchased from Vector Builder, USA). The plasmid miR-Cont (#pCMVMIR) and miR-6794-3p mimic (SC402235) were obtained from Origene, USA. The plasmids for miR-Cont (#CmiR-AN0001-SN)

(3) In vivo validation of the functional role of MicroRNA-4638-3p in breast cancer bone metastasisJournal of Cancer Research and Clinical OncologyFebruary 1, 2024R. L. Akshaya, I. Saranya, G. Margaret Salomi, P. Shanthi, R. Ilangovan, P. Venkataraman, N. Selvamuruganmetastasis. Materials and methods Transformation and restriction digestion The pCMV-MIR (empty vector; pCMV-EV; Cat #PCMVMIR, Origene, USA) and pCMV-MIR4638 (mir-4638 overexpressing vector; pCMV-miR; Cat # SC401776, Origene,

(4) LncRNA MYLK antisense RNA 1 activates cell division cycle 42/Neutal Wiskott‐Aldrich syndrome protein pathway via microRNA‐101‐5p to accelerate epithelial‐to‐mesenchymal transition of colon cancer cellsThe Kaohsiung Journal of Medical SciencesJanuary 1, 2024Zhen‐Hao Quan, Fei‐Peng Xu, Zhe Huang, Ri‐Hong Chen, Qing‐Wen Xu, Lin LinCC cells, the mature sequence of miR‐101‐5p was cloned into the pCMV‐MIR lentiviral vector (OriGene #PCMVMIR, Rockville, USA). miR‐ctrl was an unmodified pCMV‐MIR lentiviral vector. Recombinant lentiviral particles

(5) Glutathione peroxidase 2 overexpression promotes malignant progression and cisplatin resistance of KRASâ€‘mutated lung cancer cellsOncology ReportsOctober 10, 2022Mei Wang, Xu Chen, Guang Fu, Mingjian Geinserting the mature sequence of miR-325-3p (5′-AACUAUCCUCCAGGAGUUAUUU-3′) into pCMV-MIR vector (cat. no. PCMVMIR; OriGene Technologies, Inc.). The empty pCMV-MIR vector was used as the miR-ctrl. Virus particles were

(6) The Influence of MicroRNA-31 on Oxidative Stress and Radiosensitivity in Pancreatic Ductal AdenocarcinomaCellsJuly 25, 2022McGrath J, Kane LE, Maher SG.reagent (Invitrogen, Waltham, MA, USA). The MiR-31 overexpression plasmid (MI0000089) and vector control (pCMVMIR) were purchased from Origene (Herford, Germany) and stably transfected into the BxPC-3 cell line under

(7) miR-335-laden B Cell-Derived Extracellular Vesicles Promote SOX4-Dependent Apoptosis in Human Multiple Myeloma CellsJournal of Personalized MedicineNovember 23, 2021Elisabetta Lombardi et al.SgfI/XhoI ends by Integrated DNA Technologies (IDT, Coralville, IA, USA). The construct was cloned into the pCMVmir expression vector (Origene, Rockville, MD, USA) by digestion with SgfI and XhoI, and subsequent ligation

(8) Exosomal transfer of miR‑25‑3p promotes the proliferation and temozolomide resistance of glioblastoma cells by targeting FBXW7International Journal of OncologyJuly 9, 2021Jianxin Wang, Tianxiao Li, Bin Wanginserting the miR-25-3p mature sequence (5′-CAT TGC ACT TGT CTC GGT CTG A-3′) into the pCMV-MIR vector (#PCMVMIR; OriGene Technologies, Inc.). Empty pCMV-MIR vector was regarded as miR-ctrl. Single-guide RNA (sgRNA)

(9) LINC01287 facilitates proliferation, migration, invasion and EMT of colon cancer cells via miR-4500/MAP3K13 pathwayBMC cancerJuly 6, 2021Fu D, Ren Y, Wang C, Yu L, Yu R.cells, the mature sequences for miR-4500 were cloned into the pCMV-MIR lentiviral vector (OriGene #PCMVMIR, Rockville, Maryland, USA). The miR-ctrl was unmodified pCMV-MIR lentiviral vector. To knock down miR-4500

(10) Inhibitory role of ginsenoside Rb2 in endothelial senescence and inflammation mediated by microRNA‑216aMolecular medicine reportsJune 1, 2021Chen Y, Wang S, Yang S, Li R, Yang Y, Chen Y, Zhang W.

(11) Propofol modulates the proliferation, invasion and migration of bladder cancer cells through the miR‑145‑5p/TOP2A axisMolecular medicine reportsJune 1, 2021Du Y, Zhang X, Zhang H, Chen Y, Zhu S, Shu J, Pan H.cloning the mature sequence (5′-GTCCAGTTTTCCCAGGAATCCCT-3′) into a pCMV-MIR lentiviral vector (cat. no. PCMVMIR; OriGene Technologies, Inc.). Empty pCMV-MIR lentiviral plasmid was used as an empty vector control

(12) Horizontal Transfer of miR-643 from Cisplatin-Resistant Cells Confers Chemoresistance to Recipient Drug-Sensitive Cells by Targeting APOL6CellsMay 28, 2021Raji GR, Poyyakkara A, Krishnan AK, Maurya AK, Changmai U, Shankar SS, Kumar VBS.were transfected with miRNA overexpressing plasmid (miR-643) along with proper empty vector control (pCMVmiR obtained from OriGene) using polyethyleneimine method. Then, 24 h post transfection, cells were treated

(13) Epithelial-mesenchymal transition sensitizes breast cancer cells to cell death via the fungus-derived sesterterpenoid ophiobolin AScientific reportsMay 20, 2021Reisenauer KN, Tao Y, Das P, Song S, Svatek H, Patel SD, Mikhail S, Ingros A, Sheesley P, Masi M, et al.

(14) Gene-activated matrix harboring a miR20a-expressing plasmid promotes rat cranial bone augmentationRegenerative biomaterialsMarch 1, 2021Shido R, Sumita Y, Hara M, Iwatake M, Narahara S, Umebayashi M, Miura KI, Kodama Y, Asahina I.(IRES)-green fluorescent protein (GFP) (MI0000638) (SC403217), and its control vector, pCMV-mir-IRES-GFP (PCMVMIR), were purchased (Origene Technologies, Rockville, MD, USA). Isolation of mesenchymal stem cells from

(15) MiR-200c-3p Contrasts PD-L1 Induction by Combinatorial Therapies and Slows Proliferation of Epithelial Ovarian Cancer through Downregulation of β-Catenin and c-MycCellsMarch 1, 2021Anastasiadou E, Messina E, Sanavia T, Mundo L, Farinella F, Lazzi S, Megiorni F, Ceccarelli S, Pontecorvi P, Marampon F, et al.× 106 ) were placed in a 12-well cell culture plate. After 24 h, they were transfected with 1 µg of pCMVmiR-200c carrying the miR-200c precursor (pre-miR200c) (OriGene Technologies, Inc., Rockville, USA; Cat.n.SC400258)

(16) miR-375- and miR-1-Regulated Coxsackievirus B3 Has No Pancreas and Heart Toxicity But Strong Antitumor Efficiency in Colorectal CarcinomasHuman gene therapyFebruary 1, 2021Hazini A, Dieringer B, Pryshliak M, Knoch KP, Heimann L, Tolksdorf B, Pappritz K, El-Shafeey M, Solimena M, Beling A, et al.

(17) Long non-coding RNA LINC00665 promotes gemcitabine resistance of Cholangiocarcinoma cells via regulating EMT and stemness properties through miR-424-5p/BCL9L axisCell death & diseaseJanuary 12, 2021Lu M, Qin X, Zhou Y, Li G, Liu Z, Geng X, Yue H.miR-424-5p was constructed by cloning the mature sequence into pCMV-MIR lentiviral vector (OriGene #PCMVMIR). The empty pCMV-MIR lentiviral plasmid was used as miR-ctrl. For lentivirus transfection, cells were

(18) Restoration of miR-330 expression suppresses lung cancer cell viability, proliferation, and migrationJournal of cellular physiologyJanuary 1, 2021Mohammadi A, Mansoori B, Duijf PHG, Safarzadeh E, Tebbi L, Najafi S, Shokouhi B, Sorensen GL, Holmskov U, Baradaran B.3×105 cells per well were seeded in a 6-well plate. One well was cultured in order to be transfected by PCMVmiR-330 (Origene, USA) referred to as Vec+, and the other wells were cultured with empty PCMV cells as a negative

(19) CtIP suppresses primary microRNA maturation and promotes metastasis of colon cancer cells in a xenograft mouse modelThe Journal of biological chemistryJanuary 1, 2021Ren J, Wu Y, Wang Y, Zhao Y, Li Y, Hao S, Lin L, Zhang S, Xu X, Wang H.

(20) MiR-215-5p Reduces Liver Metastasis in an Experimental Model of Colorectal Cancer through Regulation of ECM-Receptor Interactions and Focal AdhesionCancers (Basel)November 26, 2020Tana Machackova, Petra Vychytilova-Faltejskova, Kamila Souckova, Karolina Trachtova, Dominika Brchnelova, Marek Svoboda, Igor Kiss, Vladimir Prochazka, Zdenek Kala, Ondrej Slabyvector (OriGene Technologies, Rockville, MD, USA, CAT#: SC400278) and empty pCMV-MIR vector (CAT#: PCMVMIR). The first selection was performed using 400 µg/mL−1 of G418 antibiotics (Sigma Aldrich, Saint Louis,

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