Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

Product Specs
ItemProtease/Phosphatase Inhibitor Cocktail (100X)
CompanyCell Signaling Technology
Price $326.00 Inquire
Catalog Number5872S
Quantity1 ml
Research AreaRelated Products
ApplicationsW
Clone100X

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Discovery of novel murine PML isoformsNucleusDecember 31, 2026Karolína Anderová, Lenka Horníková, Vojtěch Šroller, Boris Ryabchenko, Dalibor Pánek, Prokopis C. Andrikopoulos, Jiří Zahradník, Jitka Forstová, Sandra Huérfanosupplemented with a protease and phosphatase inhibitor cocktail (Cell Signaling Technology, Cat. No. 5872S) and 20 mM N-ethylmaleimide [45] (Merck) for 20 min on ice. Cellular debris was removed by centrifugation

(2) Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptakeMaterials Today BioJune 1, 2026Fang Lin, Jing Liu, Yue Ding, Kexin Ma, Qingshu Meng, Xiaohui Zhou, Qingliu Zhang, Hao Hu, Zhongmin Liu, Xiaoting Liangin RIPA buffer (CST, Cat#9806) supplemented with protease/phosphatase Inhibitor Cocktail (CST, Cat#5872). For mitochondrial protein extraction, the mitochondrial pellet obtained from the final step of the

(3) OptoTAT reveals microtubule acetylation as a rapid trigger for GEF-H1-mediated cell migrationJ Cell BiolMay 4, 2026Abhijit Deb Deb Roy, Cristian Saez Gonzalez, Milda Stanislauskas, Farid Shahid, Eesha Yadav, Jalil Rezek, Takanari Inouebuffer, Cell Signalling # 9806S), mixed with protease/phosphatase inhibitor cocktail (Cell Signaling # 5872S). Cell lysates were rotated on a wheel at 4°C for 15 min and centrifuged for 10 min at 15,000 g 4°C

(4) Human ISG15 deficiency unveils impaired healing of ulcerations via type I interferon–mediated fibrosisJournal of Human ImmunityMay 4, 2026Christos Sazeides, Lorenzo Cuollo, Ikjot Sidhu, Haley E. Randolph, Marta Martin-Fernandez, Sofija Buta, O’ Jay Stewart, Rachel Geltman, Jonas A. Adalsteinsson, Robert G. Phelps, et al.(89900; Thermo Fisher Scientific) with Dithiothreitol (DTT) and protease/phosphatase inhibitor cocktail (#5872; Cell Signaling) for at least 20 min on ice. Samples were centrifuged to remove insoluble complexes

(5) RK-33 inhibits the OC43 coronavirus and induces stress granules via DDX3X-independent mechanismsRNAMay 1, 2026Cody J.S. Hecht, Roy R. ParkerPBS and lysed with RIPA buffer (Thermo 89900) containing 1× protease and phosphatase inhibitors (CST 5872S). Soluble proteins were separated by centrifugation and separated on 4%–12% BIS-Tris PAGE gels. Proteins

(6) Nitric oxide inhibition ameliorates cortical proteomic changes in the Cntnap2-/- and Shank3Δ4-22 mouse models of autism spectrum disorderMol AutismApril 20, 2026Wisam Bazbaz, Maryam Kartawy, Igor Khaliulin, Haitham Amal(#7076S) and HRP-conjugated anti-mouse (#7074S), and the protease and phosphatase inhibitor cocktail (#5872), were purchased from Cell Signaling Technology (Danvers, MA, USA). All other reagents were obtained

(7) Autophagy selectively clears ER in TNF-α-induced muscle atrophyAutophagy RepApril 17, 2026Ursula K. Dueren, Alan An Jung Wei, A. Elisabeth Gressler, Simon Rapp, Oliver Popp, Robert Kerridge, Viviana Buonomo, Paolo Grumati, Philipp Mertins, Matthias Selbach, et al.HEPES at pH 7.4) supplemented with protease and phosphatase inhibitors (Cell Signaling Technology, 5872) for 30 min on ice. Lysates were cleared by centrifugation at 12,700 × g for 5 min at 4°C, and protein

(8) Creatine uptake promotes dendritic cell activation and enhances antitumor immunityiScienceApril 17, 2026Elliot Kang, James Elsten-Brown, Yu-Chen Wang, Ashley Lam, Elise Sanchez, Renee Wen, Tiffany Wang, Jennifer Chiang, Quentin Scarborough, Yan-Ruide Li, et al.89900, Thermo Fisher Scientific) supplemented with protease/phosphatase inhibitor cocktail (catalog no. 5872S, Cell Signaling Technology). Nuclear protein was extracted using the Nuclear Protein Extraction Kit

(9) Nanoparticle-delivered resiquimod induces brain tumor regression in medulloblastoma and diffuse midline glioma models by interrupting paracrine growth support and activating myeloid immune signaling and phagocytosisbioRxivApril 16, 2026Leon McSwain, Kyongtea Kim, Duhyeong Hwang, Chaemin Lim, Cynthia Winham, Jon Jacques, Elias Rosen, Sudhir Kasturi, Arpan Pradhan, Andrey Tikunov, et al.500–1000uL of RIPA + 1x protease/phosphatase inhibitors (RIPA cat CST #9806S, Ptase inhibitors cat CST #5872). Samples were sonicated thoroughly prior to centrifugation and protein quantification using BCA assay.

(10) Redox imbalance dictates dependence on GOT1 versus GOT2 for rod photoreceptor health during aging and stressbioRxivApril 7, 2026Meini Chen, Eric Weh, Moloy T. Goswami, Katherine M. Weh, Heather Hager, Peter Sajjakulnukit, Avi Weingarten, Shubha Subramanya, Nicholas Miller, Sraboni Chaudhury, et al.#89900) containing protease and phosphatase inhibitors (Cell Signaling Technology, Denver, MA, USA; Cat #5872S). Immunoblots were performed as previously described [42]. Briefly, samples were homogenized, centrifuged

(11) TROP2/claudin program mediates immune exclusion to impede checkpoint blockade in breast cancerJ Immunother CancerApril 3, 2026Bogang Wu, Win Thant, Elena Bitman, Ting Liu, Jie Liu, Eleftherios I Paschalis, Bidish K Patel, Cole Nawrocki, Katherine H Xu, Linda T Nieman, et al.with IP lysis buffer including protease/phosphatase inhibitor cocktail (Cell Signaling Technology, 5872) for 10 min. The lysates were centrifuged at 13,000 g for 10 min at 4° to remove debris. The lysate

(12) Gait analysis for functional evaluation in a surgical hindlimb suspension model of muscle atrophyThe Journal of PhysiologyApril 1, 2026Yanping Xu, Zhentao Zhang, Peng Chen, Natalie Kellon, Ashley He, Usman Alizai, Sunjoon Lee, Keerthika Sathish, Zhiyu Yan, Xinyu Zhou, et al.deoxycholate, prepared in‐house] supplemented with protease/phosphatase inhibitor cocktail (100X) (catalog. no. 5872s; Cell Signaling Technology, Danvers, MA, USA). Equal protein amounts were separated by 10% SDS‐polyacrylamide gel electrophoresis (PAGE

(13) RASA2 deletion rescues immune synapse dysfunction, enhancing CAR T cell efficacy against DMGsJournal for ImmunoTherapy of CancerMarch 30, 2026Jorge Ibanez-Vega, Robert Teis, Jennifer K Ocasio, Peter Chockley, Aamir Ansari, Alejandro Allo Anido, Sanya Mehta, Michaela Meehl, Brooke Prinzing, Meghan Ward, et al.RIPA buffer (Thermofisher; 89900) supplemented with protease phosphatase inhibitor cocktail (Cell Signaling; 5872S), and incubated at 4°C for 30 min. Cell lysates were centrifuged at 10 000 g for 10 min at 4°C and supernatant

(14) Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanomaJ Immunother CancerMarch 27, 2026Mark Gregory, Justin B Hackett, Savannah Hughes, Maria C Muñiz, Samantha Ayoub, Alexander Boerner, Habib Alrabaa, Lucien Taylor, Brianna Rivera, Hanna Holland, et al.(Cell Signaling Tech, cat. #9806S) with protease/phosphatase inhibitor (Cell Signaling Tech, cat. #5872) using a Tissue-Tearor. Lysates were collected after centrifugation at 15,000 g for 5 min. Protein concentration

(15) Polycystin-1 Orchestrates Tumor Context-Dependent Mechanotransduction Programs Driving Epithelial-to-Mesenchymal Transition and Invasion in Solid CancersInt J Biol SciMarch 25, 2026Antonios N. Gargalionis, Kostas A. Papavassiliou, Angeliki-Ioanna Giannopoulou, Panagiotis Sarantis, Christina Piperi, Eleftheria Lakiotaki, Anastasios Stofas, Christos Adamopoulos, Panagiota Kontou, Efstathios Boviatsis, et al.(Cell Signaling Technology #9803) supplemented with Protease/Phosphatase Inhibitor Cocktail (Cell Signaling Technology #5872). Proteins were resolved by electrophoresis in 8-10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis

(16) Bile acids enrichment fuel tumor aerobic glycolysis and immune evasion via stabilizing FXR-RARαFrontiers in ImmunologyMarch 25, 2026Hongyu Jiang, Wenyan Xiong, Yingna Feng, Xingjie Li, Jinzhuo Tan, Zongde Zhangand lysed on ice with RIPA Buffer (#9806S, CST) containing Protease/Phosphatase Inhibitor Cocktail (#5872S, CST) and 1 mM PMSF (ST505, Beyotime). Cell extracts were loaded onto Biofuraw™ Precast Gel (D0174S,

(17) In‑Situ ssDNA Isolation from dsDNA Sources as a Streamlined Pathway to DNA Origami Assembly and TestingbioRxivMarch 25, 2026Enrique O. Ruiz, Kayla Neyra, Diana M. Lopez, Ruo-Wen Chen, Deepta Paramasamy, Quincy Bizjak, Patrick D. Halley, Yin Wei, Marcos Sotomayor, Michael G. Poirier, et al.150 mM NaCl, 50 mM Tris, 1 mM EDTA, and 1:100 protease/phosphatase inhibitor (Cell Signaling, cat. #5872S) in Nuclease-Free HyPure Molecular Biology Grade Water was prepared for cell lysis. IP buffer (50 μL)

(18) Macrophage SH3 domain‑containing GRB2‑like protein B1 is required for Rab7‑mediated mitophagy in inflammation during sea water drowning acute lung injuryInt J Mol MedMarch 24, 2026Hongjuan Ning, Jingyu Deng, Yongheng Gao, Wei Zhang, Jian Chen, Xian Guo, Faguang Jinglycerol; cat. no. 87787; Thermo Fisher Scientific, Inc.) plus protease/phosphatase inhibitors (cat. no. 5872; CST Biological Reagents Co., Ltd.). Lysates were rotated overnight at 4°C with 1 µg antibody, then

(19) Glomerular basement membrane structural integrity dictates trans-tissue deposition of laminin in the kidneyCell ReportsMarch 24, 2026Kohei Omachi, Meei-Hua Lin, Pongpratch Puapatanakul, Joshua F. Begin, Karen K. McKee, Hironobu Fujiwara, Peter D. Yurchenco, Jeffrey H. MinerFisher Scientific) on ice in RIPA buffer containing protease/phosphatase inhibitor (Cell Signaling, #5872S), followed by rocking for 2 h at 4C and centrifugation for 10 min at 14K rpm at 4C. The supernatant

(20) APC coordinates GSK3 phosphorylation of SETD8 to suppress colorectal cancerCell ReportsMarch 24, 2026Zvi Cramer, Keara Monaghan, Ricardo Petroni, Xin Wang, Stephanie Adams-Tzivelekidis, Kayla Durning, Melissa S. Kim, Yuhua Tian, Nicolette M. Johnson, María F. Carrera Rodríguez, et al.(Abcam ab156034) supplemented with a protease/phosphatase inhibitor cocktail (Cell Signaling Technology #5872S), sonicated and spun down for 15 min at 14000g at 4°C. Protein concentration of resulting supernatant

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Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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Protease/Phosphatase Inhibitor Cocktail (100X) from Cell Signaling Technology

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