SignalSilence ® Atg5 siRNA I from Cell Signaling Technology

Product Specs
ItemSignalSilence ® Atg5 siRNA I
CompanyCell Signaling Technology
Price $369.00 Inquire
Catalog Number6345S
Quantity300 µl
TargetATG5
NCBI Gene AliasesAPG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5
NCBI Full Gene Nameautophagy related 5
Research AreaAutophagy
ReactivityH
ApplicationssiRNA
TypesiRNA

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Anticancer Activity of Delta-Tocotrienol in Human Hepatocarcinoma: Involvement of Autophagy InductionCancersJuly 26, 2024Montagnani Marelli M, Macchi C, Ruscica M, Sartori P, Moretti RM.(siRNA) To silence endogenous ATG5 expression, negative control (NC) siRNA (#6568) and ATG5 siRNA I (#6345) obtained from Cell Signaling were used. HepG2 were seeded in 6-well culture plates at a concentration

(2) Spermidine improves angiogenic capacity of senescent endothelial cells, and enhances ischemia-induced neovascularization in aged miceScientific ReportsMay 23, 2023Daisuke Ueno, Koji Ikeda, Ekura Yamazaki, Akiko Katayama, Ryota Urata, Satoaki Matobafor ATG5 ECs were transfected with 100 nM ATG5 (SignalSilence Atg5 siRNA, Cell Signaling Technology #6345) or negative siRNA (siRNA Universal Negative control#1, Sigma #SIC001) using Lipofectamine RNAiMAX reagent

(3) A Feedback Loop between TGF-β1 and ATG5 Mediated by miR-122-5p Regulates Fibrosis and EMT in Human Trabecular Meshwork CellsCurrent issues in molecular biologyMarch 13, 2023Chakraborthy M, Rao A.previously described [25]. Briefly, 60 pmol/well of siRNAs against autophagy-essential ATG5 (siATG5) (6345S, Cell signalling technology, Beverly, MA, USA)and nontargeting siRNA (negative control, siNC) (6568S,

(4) Porcine epidemic diarrhoea virus (PEDV) infection activates AMPK and JNK through TAK1 to induce autophagy and enhance virus replicationVirulenceDecember 31, 2022Jingxiang Wang, Xianjin Kan, Xiaomei Li, Jing Sun, Xiulong Xu(Invitrogen Life Technologies, Grand Island, NY, USA). TAK1 (#6317S), Beclin-1 (#6222S), and ATG5 (6345S) siRNA were purchased from Cell Signalling Technology (Danvers, MA, USA). Transfection of Vero cells

(5) HSPB8 counteracts tumor activity of BRAF- and NRAS-mutant melanoma cells by modulation of RAS-prenylation and autophagyCell death & diseaseNovember 18, 2022Cristofani R, Piccolella M, Montagnani Marelli M, Tedesco B, Poletti A, Moretti RM.cells were transfected with 20 nM negative control siRNA (NC siRNA) (#6568) and 20 nM ATG5 siRNA I (#6345) (Cell Signaling) by Lipofectamine RNAi max Reagent (13778) (Invitrogen-ThermoFisher Scientific) according

(6) Curcumin Blunts IL-6 Dependent Endothelial-to-Mesenchymal Transition to Alleviate Renal Allograft Fibrosis Through Autophagy ActivationFrontiers in immunologyJanuary 1, 2021Zhou J, Yao M, Zhu M, Li M, Ke Q, Wu B, Wang D.in 6-well plates with non-proliferative medium. The HUVECs were transfected with 25nM of ATG5 siRNA (6345, Cell Signaling Technology; Boston, USA) or control siRNA using Lipofectamine 2000 transfection reagent

(7) Disrupting Plasmodium UIS3-host LC3 interaction with a small molecule causes parasite elimination from host cellsCommunications biologyNovember 19, 2020Setua S, Enguita FJ, Chora ÂF, Ranga-Prasad H, Lahree A, Marques S, Sundaramurthy V, Mota MM.with LC3B siRNA (Cell Signalling Technology, 6213, 100 nM) or Atg5 siRNA (Cell Signalling Technology, 6345, 100 nM) using Lipofectamine RNAiMAX as per manufacturer protocol. The efficiency of knockdown was assessed

(8) Pyrolysis of Plastics to Liquid Fuel Using Sulphated Zirconium Hydroxide CatalystWaste and Biomass ValorizationNovember 1, 2020Achyut K. Panda, Abdullah Alotaibi, Ivan V. Kozhevnikov, N. Raveendran Shiju15] respectively whereas the sulphated 13 6344 Waste and Biomass Valorization (2020) 11:6337–6345 Table 3 Fuel properties of the oil obtained from plastics Parameters/test protocol Colour Specific

(9) Amino Acid Starvation Sensitizes Resistant Breast Cancer to Doxorubicin-Induced Cell DeathFrontiers in Cell and Developmental BiologyOctober 15, 2020Mark Thomas, Tanja Davis, Theo Nell, Balindiwe Sishi, Anna-Mart Engelbrechtdishes. ATG5 siRNA duplex (20 pmol, Cell Signaling, Danvers, MA, United States, lSilence® Atg5 siRNA I #6345) was diluted into 250 μl transfection medium (containing no antibiotics or serum), where after 2 μl

(10) Flubendazole elicits anti-cancer effects via targeting EVA1A-modulated autophagy and apoptosis in Triple-negative Breast CancerTheranosticsJanuary 1, 2020Zhen Y, Zhao R, Wang M, Jiang X, Gao F, Fu L, Zhang L, Zhou XL.sequences of the siRNA or cDNA involved in this study listed in Supplementary table 2. si-ATG5 siRNA (6345, CST) and Control siRNA (6568, CST) were purchased from CST. The RNA was transfected with Lipofectamine 3000

(11) Resveratrol represses tumor necrosis factor α/c-Jun N-terminal kinase signaling via autophagy in human dental pulp stem cellsArchives of oral biologyJanuary 1, 2019Wang FM, Hu Z, Liu X, Feng JQ, Augsburger RA, Gutmann JL, Glickman GN.treatments at the concentration of 2.5 ng/mL. 2.3. RNA interference SignalSilence® Atg5 siRNA I (#6345) and control (#6568) were purchased from Cell Signaling Technology (Beverly, MA, USA). siRNAs were introduced

(12) ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cellsScientific reportsNovember 9, 2018Chen W, Nyuydzefe MS, Weiss JM, Zhang J, Waksal SD, Zanin-Zhorov A.

(13) Dual role of autophagy on docetaxel-sensitivity in prostate cancer cellsCell death & diseaseAugust 30, 2018Cristofani R, Montagnani Marelli M, Cicardi ME, Fontana F, Marzagalli M, Limonta P, Poletti A, Moretti RM.transfections. To silence endogenous ATG5 expression we used negative control (NC) siRNA (#6568) and ATG5 siRNA I (#6345) obtained from Cell Signaling (#6345). Cell culture and treatments Human PC cell lines, LNCaP, PC3

(14) Promyelocytic Leukemia Restricts Enterovirus 71 Replication by Inhibiting AutophagyFrontiers in immunologyJanuary 1, 2018Chen D, Feng C, Tian X, Zheng N, Wu Z.interfering RNA (siRNA) (sc-36284) was purchased from Santa Cruz (Santa Cruz, CA, USA). Atg5 siRNA (#6345) and a non-specific scrambled siRNA (#6586) were obtained from CST (Cell Signaling Technology, USA). Rhabdomyosarcoma

(15) Cytokine-induced autophagy promotes long-term VCAM-1 but not ICAM-1 expression by degrading late-phase IκBαScientific ReportsDecember 1, 2017Ling-Yun Chu, Ying-Chang Hsueh, Hsiao-Ling Cheng, Kenneth K. Wunm/520 nm in the lysates with a Synergy H1 Hybrid Reader (BioTek). siRNA transfection Atg5 siRNA (#6345) was purchased from Cell Signaling Technology. Control siRNA (AM4611) was purchased from Thermo Fisher Scientific

(16) A Unified Approach to Targeting the Lysosome's Degradative and Growth Signaling RolesCancer DiscoveryNovember 1, 2017Vito W. Rebecca et al.Cell Signaling Technology SignalSilence® Rheb siRNA I (#14267) and siRNA II (#14284), mTOR siRNA (#6381S), and ATG5 siRNA (#6345), along with Control siRNA (#6568) per manufacturers protocol. Electron Microscopy Electron microscopy

(17) Synthetic lethal interaction of CDK inhibition and autophagy inhibition in human solid cancer cell linesOncology reportsJuly 1, 2017Okada Y, Kato S, Sakamoto Y, Oishi T, Ishioka C.BECN1, and negative control siRNAs were purchased from Signal Silence, Cell Signaling Technology (#6345, #6222, and #6568). Each siRNA was introduced to the cell cultures to make a final concentration of

(18) Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase MProceedings of the National Academy of Sciences of the United States of AmericaApril 25, 2017Hansen MD, Johnsen IB, Stiberg KA, Sherstova T, Wakita T, Richard GM, Kandasamy RK, Meurs EF, Anthonsen MW.(Dharmacon; J-005049-06-0020), ULK2 (Dharmacon; LQ-005396-00-0005), ATG5 (Cell Signaling Technology; 6345), IRGM (Ambion; s51146 and s51147), Arf1 (Dharmacon; J-011580-06-0005), GBF1 (Dharmacon; J-019783-06-0005),

(19) The Contribution of Melanoregulin to Microtubule-Associated Protein 1 Light Chain 3 (LC3) Associated Phagocytosis in Retinal Pigment EpitheliumMolecular neurobiologyDecember 1, 2015Frost LS, Lopes VS, Bragin A, Reyes-Reveles J, Brancato J, Cohen A, Mitchell CH, Williams DS, Boesze-Battaglia K.manufacturer’s instructions, (Invitrogen)] with 100 pmol of ATG5 siRNA I (Cell Signaling Technologies # 6345S) or 100 pmol of Silencer Negative Control siRNA (Invitrogen # AM4611) and cells analyzed 72-h post transfection.

(20) Andrographolide Analogue Induces Apoptosis and Autophagy Mediated Cell Death in U937 Cells by Inhibition of PI3K/Akt/mTOR PathwayPloS oneJanuary 1, 2015Kumar D, Das B, Sen R, Kundu P, Manna A, Sarkar A, Chowdhury C, Chatterjee M, Das P.(3438), PI3K(p85) (4257), p-PI3Kp85(Tyr458)/p55(Tyr199) (4228), LY294002 (9901) and siRNA against Atg 5 (6345), Bax (6321), Akt (6211), mTOR (6381) and siControl (6568) were procured from Cell Signaling Technology

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