SignalSilence ® Akt siRNA I from Cell Signaling Technology

Product Specs
ItemSignalSilence ® Akt siRNA I
CompanyCell Signaling Technology
Price $369.00 Inquire
Catalog Number6211S
Quantity300 µl
TargetAkt2;Akt3;Akt1
ReactivityH
ApplicationssiRNA
TypesiRNA
NCBI Full Gene NameAKT serine/threonine kinase 3, AKT serine/threonine kinase 2, AKT serine/threonine kinase 1
NCBI Gene AliasesPKB, RAC, PKB-GAMMA, PRKBB, PKB-ALPHA, PRKBA, STK-2, PRKBG, RAC-gamma, HIHGHH, RAC-ALPHA, MPPH2, MPPH, PKBG, AKT, RAC-PK-gamma, RAC-BETA, PKBBETA, PKBB
Research AreaPI3K / Akt Signaling
Molecular Weight60

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Akt inhibition enhances the antitumor efficacy of immune checkpoint blockades and radiotherapy in a syngeneic breast cancer modelMolecular Therapy OncologyDecember 18, 2025Nawon Park, Seung Hyuck Jeon, Yoomin Kim, Seongmin Kim, In Ah KimsiRNA (Cell Signaling Technology, USA, 6568s) or Akt-specific siRNA (Cell Signaling Technology, USA, 6211s) for 36 h. After treatment, cells were harvested and prepared for flow cytometry or western blot analysis.

(2) K-Ras(V12) differentially affects the three Akt isoforms in lung and pancreatic carcinoma cells and upregulates E-cadherin and NCAM via Akt3Cell Communication and SignalingJanuary 30, 2024Rebekka Geißert, Angela Lammert, Stefanie Wirth, Rabea Hönig, Dirk Lohfink, Monika Unger, Denis Pek, Konstantin Schlüter, Theresa Scheftschik, Daniel J. Smit, et al.Groningen, Netherlands), 66 nM siAkt or fluorescein-conjugated control siRNA (SignalSilencer Akt siRNA I, #6211, SignalSilencer Control siRNA #6201; Cell Signaling Technology, Leiden, Netherlands), and 375 μl growth

(3) Akt, IL-4, and STAT Proteins Play Distinct Roles in Prostaglandin Production in Human Follicular Dendritic Cell-like CellsInternational journal of molecular sciencesNovember 24, 2023Jeong J, Choe J.anti-CD38 (clone HIT2) antibodies were products of BD Biosciences (San Jose, CA, USA). Akt siRNA (#6211) was obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). siRNAs against STAT1 (4390824-s277),

(4) Combined inhibition of ACLY and CDK4/6 reduces cancer cell growth and invasionOncology reportsFebruary 1, 2023Velez BC, Petrella CP, DiSalvo KH, Cheng K, Kravtsov R, Krasniqi D, Krucher NA.Health). Transfection MDA-MB-231 or Panc1 cells were transfected with AKT small interfering RNA (cat. no. 6211; Cell Signaling Technology, Inc.) or NT RNA as a control (cat. no. 6568; Cell Signaling Technology,

(5) The role of Eya1 and Eya2 in the taste system of mice from embryonic stage to adulthoodFrontiers in cell and developmental biologyJanuary 1, 2023Zhang T, Xu PX.antibodies used were before IHC rabbit anti-Sox2 (PA1-094, ThermoFisher), rabbit anti-Six1 (50-204-6211, Cell Signaling), mouse anti-TUBB3 (66375-1-Ig, Proteintech), rabbit anti-β-Gal (08559761, MP Biomedicals),

(6) Genetic impairment of succinate metabolism disrupts bioenergetic sensing in adrenal neuroendocrine cancerCell reportsAugust 16, 2022Gupta P, Strange K, Telange R, Guo A, Hatch H, Sobh A, Elie J, Carter AM, Totenhagen J, Tan C, et al.N/A Rabbit anti-ChrA,Abcam,Cat#15160; RRID: AB_368477 Rabbit anti-Tyrosine Hydroxylase,Abcam,Cat#6211; RRID: AB_2240393 Rabbit anti-GFP,Cell Signaling Technology,Cat#2956; RRID: AB_1196615 Rabbit anti-p35/25,Cell Signaling Technology

(7) Loss of ZNF677 Expression Is an Independent Predictor for Distant Metastasis in Middle Eastern Papillary Thyroid Carcinoma PatientsInternational journal of molecular sciencesJuly 22, 2021Siraj AK, Poyil PK, Parvathareddy SK, Alobaisi K, Ahmed SO, Al-Sobhi SS, Al-Dayel F, Al-Kuraya KS.(3396), MMP-2 (13132), MMP-9 (2270), PARP (9542), and cleaved caspase-3 (9664) as well as AKT siRNAs (6211 and 6510) were purchased from Cell Signaling Technologies (Danvers, MA, USA). N-cadherin (ab98952) and

(8) Simultaneous Activation of Erk1/2 and Akt Signaling is Critical for Formononetin-Induced Promotion of Endothelial FunctionFrontiers in pharmacologyJanuary 1, 2020Wu J, Kong M, Lou Y, Li L, Yang C, Xu H, Cui Y, Hao H, Liu Z.control siRNA (siCon, #6568), Erk1/2-specific siRNA (siErk1/2, #6560), and Akt-specific siRNA (siAkt, #6211) were from Cell Signaling Technology Inc. (Beverly, MA). All other chemicals were of analytical reagent

(9) Targeting secretory leukocyte protease inhibitor (SLPI) inhibits colorectal cancer cell growth, migration and invasion via downregulation of AKTPeerJJanuary 1, 2020Wei Z, Liu G, Jia R, Zhang W, Li L, Zhang Y, Wang Z, Bai X.within its facilities. siRNA-mediated gene knockdown Cell Signaling Technology provided siAkt (No. 6211). The siRNAs for SLPI was designed by online software (http://rnaidesigner.invitrogen.com/) and shown

(10) Circular RNA hsa_circ_0006848 Related to Ribosomal Protein L6 Acts as a Novel Biomarker for Early Gastric CancerDisease MarkersSeptember 2, 2019Jun Lu, Peng-yang Zhang, Jian-wei Xie, Jia-bin Wang, Jian-xian Lin, Qi-yue Chen, Long-long Cao, Ping Li, Chao-hui Zheng, Chang-ming HuangCell Signaling Technology. Anti-GAPDH (ab181602) antibodies were purchased from Abcam. AKT siRNA (#6211) and control siRNA (#6568) were purchased from Cell Signaling Technology. We corrected the loading error

(11) Akt Regulates a Rab11-Effector Switch Required for CiliogenesisDevelopmental cellJuly 22, 2019Walia V, Cuenca A, Vetter M, Insinna C, Perera S, Lu Q, Ritt DA, Semler E, Specht S, Stauffer J, et al.2010",Custom/ OTP siRNA targeting sequence: sipanAKTsequence not available from company,Cell Signaling,6211 siRNA targeting sequence: siFIP1 #1AAACAGAAGGAAACGAUAA,Dharmacon,J-015968-08 siRNA targeting sequence:

(12) AKT inhibition impairs PCNA ubiquitylation and triggers synthetic lethality in homologous recombination-deficient cells submitted to replication stressOncogeneMay 1, 2019Villafañez F, García IA, Carbajosa S, Pansa MF, Mansilla S, Llorens MC, Angiolini V, Guantay L, Jacobs H, Madauss KP, et al.(ID#1864). The siRNA duplexes used (Cell Signaling Technology) were: siSCR (control) 6568S and siAKT 6211S. Antibodies Primary antibodies used were: α-ubiquityl-PCNA (D5C7P; Cat# 13439), α-PCNA (PC-10; Cat#

(13) CDK5RAP3 suppresses Wnt/β-catenin signaling by inhibiting AKT phosphorylation in gastric cancerJournal of Experimental & Clinical Cancer ResearchDecember 1, 2018Chao-Hui Zheng et al.(ab76057), Vimentin (ab8978), and GAPDH (ab181602) antibodies were purchased from Abcam. AKT siRNA (#6211) and control siRNA (#6568) were purchased from Cell Signaling Technology. We corrected the loading error

(14) PI3K/AKT inhibition induces compensatory activation of the MET/STAT3 pathway in non‑small cell lung cancerOncology LettersApril 26, 2018Chunan Bian, Zonghang Liu, Dakou Li, Lifeng Zheninterfering RNAs (siRNAs) for p110α (si-p110α; 100 nM; cat. no. 6359S), AKT (si-AKT; 100 nM; cat. no. 6211S) and MET (si-MET; 100 nM; cat. no. 6618S) were obtained from Cell Signaling Technology Inc. (Danvers,

(15) AMPK–Akt Double-Negative Feedback Loop in Breast Cancer Cells Regulates Their Adaptation to Matrix DeprivationCancer ResearchMarch 15, 2018Manipa Saha, Saurav Kumar, Shoiab Bukhari, Sai A. Balaji, Prashant Kumar, Sravanth K. Hindupur, Annapoorni Rangarajanplasmids in pTRIPZ vector) expression. siRNA oligos against Akt (targeting both isoforms Akt1 and Akt2 [6211 and 6510]) were purchased from Cell Signaling Technology and transfected using oligofectamine (Invitrogen). Pharmacologic

(16) AMPK–Akt Double-Negative Feedback Loop in Breast Cancer Cells Regulates Their Adaptation to Matrix DeprivationCancer Res.January 16, 2018Manipa Saha, Saurav Kumar, Shoiab Bukhari, Sai A. Balaji, Prashant Kumar, Sravanth K. Hindupur, Annapoorni Rangarajanplasmids in pTRIPZ vector) expression. siRNA oligos against Akt (targeting both isoforms Akt1 and Akt2 [6211 and 6510]) were purchased from Cell Signaling Technology and transfected using oligofectamine (Invitrogen). Pharmacologic

(17) Dissection of Myogenic Differentiation Signatures in Chickens by RNA-Seq AnalysisGenesJanuary 11, 2018Li T, Zhang G, Wu P, Duan L, Li G, Liu Q, Wang J.

(18) Piperlongumine induces apoptosis and autophagy in leukemic cells through targeting the PI3K/Akt/mTOR and p38 signaling pathwaysOncology LettersNovember 29, 2017Hongfei Wang, Yongqiang Wang, Hongmei Gao, Bing Wang, Lin Dou, Yin Li(LC3‑I; cat. no. 3868; 1:3,000), anti‑phosphorylated (p)‑Akt (cat. no. 4228; 1:3,000), anti‑Akt (cat. no. 6211; 1:3,000), anti‑p‑mechanistic target of rapamycin (mTOR; cat. no. 2974; 1:3,000), anti‑p‑p38 (cat. no.

(19) WNT-Mediated Regulation of FOXO1 Constitutes a Critical Axis Maintaining Pubertal Mammary Stem Cell HomeostasisDevelopmental cellNovember 20, 2017Sreekumar A, Toneff MJ, Toh E, Roarty K, Creighton CJ, Belka GK, Lee DK, Xu J, Chodosh LA, Richards JS, et al.equivalent volume of DMSO. For siRNA experiments, 293T cells were transfected with 2 siRNAs against Akt (6211 & 6510, Cell Signaling Technologies) using TransIT-293 Transfection reagent (MIR 6004, Mirus) as per

(20) Mcl-1 stabilization confers resistance to taxol in human gastric cancerOncotargetOctober 10, 2017Shuang W, Hou L, Zhu Y, Li Q, Hu W.

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