SignalSilence ® Control siRNA (Fluorescein Conjugate) from Cell Signaling Technology

Product Specs
ItemSignalSilence ® Control siRNA (Fluorescein Conjugate)
CompanyCell Signaling Technology
Price $162.00 Inquire
Catalog Number6201S
Quantity60 µl
Research AreaRelated Products
ApplicationssiRNA
TypesiRNA
Conjugate/Tag/LabelFluorescein

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Interplay between B7–H3 and HLA class I in the clinical course of pancreatic ductal adenocarcinomaCancer LettersApril 1, 2024Giulia Cattaneo, Marco Ventin, Shahrzad Arya, Filippos Kontos, Theodoros Michelakos, Yurie Sekigami, Lei Cai, Vincenzo Villani, Francesco Sabbatino, Francine Chen, et al.were transfected with a NF-kB p65 siRNA (Cell Signaling, #6261) or a control siRNA (Cell Signaling, #6201) at the concentration of 100nM. Lipofectamin 3000™ transfection reagent (Thermo Fisher Scientific) was

(2) K-Ras(V12) differentially affects the three Akt isoforms in lung and pancreatic carcinoma cells and upregulates E-cadherin and NCAM via Akt3Cell Communication and SignalingJanuary 30, 2024Rebekka Geißert, Angela Lammert, Stefanie Wirth, Rabea Hönig, Dirk Lohfink, Monika Unger, Denis Pek, Konstantin Schlüter, Theresa Scheftschik, Daniel J. Smit, et al.fluorescein-conjugated control siRNA (SignalSilencer Akt siRNA I, #6211, SignalSilencer Control siRNA #6201; Cell Signaling Technology, Leiden, Netherlands), and 375 μl growth medium was used. The cells were

(3) Lung Fibroblasts Take up Breast Cancer Cell-derived Extracellular Vesicles Partially Through MEK2-dependent MacropinocytosisCancer Research CommunicationsJanuary 22, 2024Yuhao Wan, Yue Zhao, Minghui Cao, Jingyi Wang, Sheila V. Tran, Zhixuan Song, Brent W. Hsueh, Shizhen Emily Wangpurchased from Invitrogen. The control siRNA was purchased from Cell Signaling Technology (catalog no. 6201S). Plasmids encoding human wild-type MEK2 or a catalytically inactive mutant (K101A), LZRS-Mek2-wt (Addgene

(4) Genetic removal of synaptic Zn2+ impairs cognition, alters neurotrophic signaling and induces neuronal hyperactivityFrontiers in NeurologyJanuary 20, 2023Emily C. Vogler, Matthew Mahavongtrakul, Kristianna Sarkan, Ryan C. Bohannan, Silvina Catuara-Solarz, Jorge Busciglioused were mouse anti-GAPDH (Abcam 125247), rabbit anti-GAPDH (Abcam 37168), rabbit anti-BDNF (Abcam 6201), rabbit anti-MAPK (ERK1/2; Cell Signaling Technology 4695), rabbit anti-phospho-p44/42 MAPK (ERK1/2;

(5) Tumor-Derived Cell Culture Model for the Investigation of Meningioma BiologyJournal of Neuropathology & Experimental NeurologyDecember 29, 2021Erik J Uhlmann, Rosalia Rabinovsky, Hemant Varma, Rachid El Fatimy, Ekkehard M Kasper, Justin M Moore, Rafael A Vega, Ajith J Thomas, Ronald L Alterman, Martina Stippler, et al.ThermoFisher Scientific) before transfection. 0.125 nmol of Fluorescein-labeled control siRNA (No. 6201, Cell Signaling Technology, Danvers, MA) diluted in 50 μl transfection media was mixed with 3 μl transfection reagent

(6) The Post-Translational Regulation of Epithelialâ€“Mesenchymal Transition-Inducing Transcription Factors in Cancer MetastasisInternational Journal of Molecular SciencesMarch 30, 2021Eunjeong Kang, Jihye Seo, Haelim Yoon, Sayeon ChoPathways in the Epithelial-Mesenchymal Transition in Cancer. Asian Pacific J. Cancer Prev. 2015, 16, 6201–6206. [CrossRef] Perona, R. Cell signalling: Growth factors and tyrosine kinase receptors. Clin. Transl.

(7) Icariin Attenuates Amyloid-β (Aβ)-Induced Neuronal Insulin Resistance Through PTEN DownregulationFrontiers in pharmacologyJanuary 1, 2020Zou X, Feng X, Fu Y, Zheng Y, Ma M, Wang C, Zhang Y.for 10 min. Small Interfering RNA (siRNA) and Transfection PTEN siRNA (#6538) and control siRNA (#6201) were obtained from Cell Signaling Technology (Beverly, MA, USA). Transfection of siRNAs was performed

(8) TNFα promotes mucosal wound repair through enhanced platelet activating factor receptor signaling in the epitheliumMucosal immunologyJuly 1, 2019Birkl D, Quiros M, García-Hernández V, Zhou DW, Brazil JC, Hilgarth R, Keeney J, Yulis M, Bruewer M, García AJ, et al.concentration of 10 μg/ml. p65 NF-κB and control siRNA were from Cell Signaling Technologies (cat 6261S and 6201) and used as suggested by the manufacturer. The NF-κB inhibitors Flavopiridol hydrochloride (cat ab

(9) Host MicroRNA hsa-miR-494-3p Promotes EV71 Replication by Directly Targeting PTENFrontiers in Cellular and Infection MicrobiologySeptember 3, 2018Qing Zhao, Yuan Xiong, Jingru Xu, Shuang Chen, Pu Li, Yong Huang, Yunying Wang, Wei-Xian Chen, Bo Wang(MC12409), miR-494 inhibitors (MH12409), miRNA negative control (miR-NC), mutant PTEN siRNA (CST, #6201), and PTEN siRNA (CST, #6538) using Lipofectamine 2000 (Invitrogen). The miRNAs and siRNAs were commercially

(10) Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza virusesVirology journalApril 10, 2018Slater T, Eckerle I, Chang KC.using the Viromer Blue system (Lipocalyx) with a SignalSilence Control siRNA (Fluorescein Conjugate) (#6201, Cell Signaling Technology) according to manufacturer’s instructions. Flow cytometry for quantification

(11) Regulation of human glioblastoma cell death by combined treatment of cannabidiol, γ-radiation and small molecule inhibitors of cell signaling pathwaysOncotargetSeptember 26, 2017Ivanov VN, Wu J, Hei TK.control siRNA (#6568) from “Cell Signaling Technology” were used. Control siRNA (Fluorescein conjugate) (#6201) was added to both preparations (at ratio 1:5). 48 h after transfection, cells were treated by CBD for

(12) Synthetic lethality between androgen receptor signalling and the PARP pathway in prostate cancerNature communicationsAugust 29, 2017Asim M, Tarish F, Zecchini HI, Sanjiv K, Gelali E, Massie CE, Baridi A, Warren AY, Zhao W, Ogris C, et al.

(13) 3,3â€²-diindolylmethane downregulates cyclin D1 through triggering endoplasmic reticulum stress in colorectal cancer cellsOncology ReportsJanuary 1, 2017Xiaobo Zhang, Pakin Sukamporn, Shiqiang Zhang, Kyung-Won Min, Seung Joon BaekPERK and total eIF2α were obtained from Cell Signaling Technology (Danvers, MA, USA). Control siRNA (#6201) and specific siRNA against PERK (#9024) were purchased from Cell Signaling Technology. Cell culture media

(14) Truncation of the TAR DNA-binding protein 43 is not a prerequisite for cytoplasmic relocalization, and is suppressed by caspase inhibition and by introduction of the A90V sequence variantPloS oneJanuary 1, 2017Wobst HJ, Delsing L, Brandon NJ, Moss SJ.

(15) Hypoxic preconditioning enhances neural stem cell transplantation therapy after intracerebral hemorrhage in miceJournal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismDecember 1, 2016Wakai T, Narasimhan P, Sakata H, Wang E, Yoshioka H, Kinouchi H, Chan PH.transfected with 200 nM Akt siRNA (6211; Cell Signaling Technology) or nonfunctioning negative control siRNA (6201; Cell Signaling Technology), and with 200 nM HIF-1a siRNA (sc-35562; Santa Cruz Biotechnology, Santa Journal

(16) Intracellular ascorbate tightens the endothelial permeability barrier through Epac1 and the tubulin cytoskeletonAmerican journal of physiology. Cell physiologyOctober 1, 2016Parker WH, Rhea EM, Qu ZC, Hecker MR, May JM.no. 6406; Cell Signaling Technology; 10 nM) or control siRNA with fluorescein conjugate (catalog no. 6201; Cell Signaling Technology; 10 nM). siRNA was prepared in serum- and antibiotic-free HUVEC media containing

(17) Caveolin-1 regulates the expression of tight junction proteins during hyperoxia-induced pulmonary epithelial barrier breakdownRespiratory researchMay 12, 2016Xu S, Xue X, You K, Fu J.

(18) Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-κB PathwayPloS oneJanuary 1, 2016Wang XL, Zhou YL, Sun W, Li L.

(19) PI3K/Akt/p53 pathway inhibits reovirus infectionInfection, Genetics and EvolutionAugust 1, 2015Xiaozhan Zhang, Hongxia Wu, Chunguo Liu, Jin Tian, Liandong QuBiosciences). 2.7 Knockdown of PI3K p85α and Akt p85α siRNA (6231, CST), Akt siRNA (6510, CST) and control siRNA (6201, CST) were obtained from Cell Signaling Technology. A549 cells grown in 24-well plates were transfected

(20) Design of a functional cyclic HSV1-TK reporter and its application to PET imaging of apoptosisNature ProtocolsMay 1, 2015Zhe Wang, Fu Wang, Naoki Hida, Dale O Kiesewetter, Jie Tian, Gang Niu, Xiaoyuan Chen(Cell Signaling, cat. no. 6466) SignalSilence scramble siRNA (Control siRNA, Cell Signaling, cat. no. 6201) SuperScript III first-strand synthesis system (Invitrogen, cat. no. 18080-051) TRIzol RNA isolation

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