LightCycler®480 Instrument II from Roche

Product Specs
ItemLightCycler®480 Instrument II
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number05015278001
Wells96-well, 384-well
ApplicationsGene detection, Gene expression analysis, And genetic variation analysis, Multiplexing
Volume96-well PCR plate: 10 - 100 µl
384-well PCR plate: 5 - 20 µl
Time< 40 minutes (384-well PCR plate)
Excitation SourceBroad-spectrum, high-intensity LED (390 – 710 nm)
Excitation Wavelength420 nm - 670 nm
DetectionSix detection filters; Cooled CCD camera
QuantityEA
Temperature Range37 to 95 °C
Heating Rate4.4 to 4.8 °C/sec
Channels6

Features

Customizable channels and filters; Interchangeable blocks

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) miR-7 is recruited to the high molecular weight RNA-induced silencing complex in CD8+ T cells upon activation and suppresses IL-2 signalingRNAJanuary 1, 2024Matilda Toivakka, Katrina Gordon, Sujai Kumar, Roberto Roberto Bermudez-Barrientos, Cei Abreu-Goodger, Rose Zamoyska, Amy H. Buckquantified using miScript Primer Assays (Qiagen) and QuantiText SYBR Green PCR Master Mix (Qiagen) on a LightCycler 480 Instrument II (Roche). qPCR data were analyzed in Microsoft Excel. Data were first normalized to snRNA U6, then calculated

(2) Key bacterial taxa determine longitudinal dynamics of aromatic amino acid catabolism in infants’ gutGut MicrobesDecember 31, 2023Martin F. Laursen, Anurag K. Sinha, Mikael Pedersen, Henrik M. Roager(V3-region) of E. coli (ATCC 25922) into a pCR4-Blunt-TOPO vector (Invitrogen). Plates were run on the LightCycler® 480 Instrument II (Roche, 05015243001) with the program including 5 min pre-incubation at 95°C, followed by 45 cycles

(3) Identification of a novel SNP in the miR172 binding site of Q homoeolog AP2L-D5 is associated with spike compactness and agronomic traits in wheat (Triticum aestivum L.)Theor Appl GenetDecember 23, 2023Victoria Zeng, Cristobal Uauy, Yi Chenthree no template control (NTC) reactions for each biological replicate. qRT-PCR was conducted on a LightCycler 480 Instrument II (Roche) under the following conditions: initial template denaturation at 95 °C (5 min); 45 amplification

(4) Prevalence of hepatitis D virus infection among patients with chronic hepatitis B infection in a tertiary care centre in ThailandSci RepDecember 19, 2023Prooksa Ananchuensook, Sirinporn Suksawatamnuay, Panarat Thaimai, Nipaporn Siripon, Supachaya Sriphoosanaphan, Kessarin Thanapirom, Yong Poovorawan, Piyawat Komolmit× 10–2 international unit per microliter (IU/µL). The amplification process was performed using the LightCycler 480 Instrument II (Roche, Switzerland). HDV genotype sequencing Positive HDV RNA samples were used to determine the

(5) AGER-1 Long Non-Coding RNA Levels Correlate with the Expression of the Advanced Glycosylation End-Product Receptor, a Regulator of the Inflammatory Response in Visceral Adipose Tissue of Women with Obesity and Type 2 Diabetes MellitusInt J Mol SciDecember 13, 2023Klaudia Gutowska, Krzysztof Koźniewski, Michał Wąsowski, Marta Izabela Jonas, Zbigniew Bartoszewicz, Wojciech Lisik, Maurycy Jonas, Artur Binda, Paweł Jaworski, Wiesław Tarnowski, et al.to cDNA using previously described methods [19]. Real-time PCR was performed in triplicate using a LightCycler 480 Instrument II (Roche, Mannheim, Germany), as previously described [50]. The specific primers used to analyze lncAGER-1

(6) An alternative NURF complex sustains acute myeloid leukemia by regulating the accessibility of insulator regionsThe EMBO JournalDecember 11, 2023Aliaksandra Radzisheuskaya, Isabel Peña‐Rømer, Eugenia Lorenzini, Richard Koche, Yingqian Zhan, Pavel V Shliaha, Alexandra J Cooper, Zheng Fan, Daria Shlyueva, Jens V Johansen, et al.cDNA and 1× LightCycler 480 SYBR Green I Master Mix (Roche Cat. no. 04707516001) per reaction on a LightCycler 480 Instrument II (Roche). Levels of gene expression were measured by relative quantification to the expression of a housekeeping

(7) Hematopoietic stem cells undergo a lymphoid to myeloid switch in early stages of emergency granulopoiesisThe EMBO JournalDecember 1, 2023Karolina Vanickova, Mirko Milosevic, Irina Ribeiro Bas, Monika Burocziova, Asumi Yokota, Petr Danek, Srdjan Grusanovic, Mateusz Chiliński, Dariusz Plewczynski, Jakub Rohlena, et al.Quantitative RT–PCR was performed using a LightCycler® 480 SYBR Green I Master mix and samples were run on a LightCycler® 480 Instrument II (both Roche Molecular Systems, Pleasanton, CA, USA). For each sample, transcript levels of tested genes

(8) HHV-6 infections in hospitalized young children of GabonInfectionDecember 1, 2023Juliana Inoue, David Weber, José Francisco Fernandes, Ayola Akim Adegnika, Selidji Todagbe Agnandji, Bertrand Lell, Peter G. Kremsner, Martin Peter Grobusch, Benjamin Mordmüller, Jana HeldnM of each primer, 100 nM of probe and 2 µl extracted DNA. Samples were assayed in duplicates in the LightCycler® 480 Instrument II (Roche Diagnostics). All assays included plasmid containing the DNA target sequence and DNA from HHV-6

(9) An enhanced biophysical screening strategy to investigate the affinity of ASOs for their target RNA†RSC Chemical BiologyNovember 29, 2023Rouven Stulz, Michael Lerche, Olivia Luige, Agnes Taylor, Stefan Geschwindner, Alice GhidiniSilvers et al.14 All experiments were performed in a 384 qPCR plate (SP-1384, Thermo Scientific) using a LightCycler® 480 Instrument II (Roche) with PBS buffer (0.01 M phosphate buffer, 2.7 mM KCl, 137 mM NaCl, pH 7.4), or no salt-free

(10) Regulation of host gene expression by J paramyxovirusPLOS ONENovember 14, 2023Elizabeth R. Wrobel, Jared Jackson, Mathew Abraham, Biao Hereplicate (KK8421, KAPA Biosystems, Wilmington, MA, USA). The Roche LightCycler 480 II (product no. 05015278001, Roche Molecular Systems, Inc., Pleasanton, CA, USA) was used to pool all the libraries together by

(11) Pathogenicity Studies of NADC34-like Porcine Reproductive and Respiratory Syndrome Virus LNSY-GY and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus GXGG-8011 in PigletsVirusesNovember 11, 2023Hechao Zhu, Liuqing Wei, Xiangzu Liu, Shudan Liu, Huanchun Chen, Pin Chen, Xiangmin Li, Ping Qianquantitative system was prepared with FastKing (TIANGEN, Harbin, China) one-step reagents and amplified using LightCycler® 480 Instrument II (Roche, Basel, Swiss Confederation). Briefly, this method uses specific primers and fluorogenic probes

(12) Characterization of intrinsic and effective fitness changes caused by temporarily fixed mutations in the SARS-CoV-2 spike E484 epitope and identification of an epistatic precondition for the evolution of E484A in variant OmicronVirology JournalNovember 8, 2023Simon Schröder, Anja Richter, Talitha Veith, Jackson Emanuel, Luca Gudermann, Kirstin Friedmann, Lara M. Jeworowski, Barbara Mühlemann, Terry C. Jones, Marcel A. Müller, et al.(Roche). Quantification of subgenomic nucleocapsid RNA (sgRNA N) was performed by real-time PCR on a LightCycler 480 Instrument II (Roche) using Taq-Man PCR probes and primers as specified in [50]. Relative sgRNA N reads were normalized

(13) NUAK1 promotes tumor metastasis through upregulating slug transcription in esophageal squamous cell carcinomaCancer Cell InternationalNovember 2, 2023Huiru Yang, Zhen Wei, Yifan Song, Kexin Du, Nannan Yin, Hong Lu, Bingbing Li, Lili Hou, Panfei Xing, Liang Chen, et al.qRT-PCR was performed with LightCycler 480 SYBR Green I Master (Roche, Basel, Switzerland) using the LightCycler® 480 Instrument II (Roche Diagnostics AG, Rotkreuz, Switzerland). The sequences of the primers used for amplifying NUAK1,

(14) Efficacy and safety of ivermectin for the treatment of Plasmodium falciparum infections in asymptomatic male and female Gabonese adults – a pilot randomized, double-blind, placebo-controlled single-centre phase Ib/IIa clinical trialeBioMedicineNovember 1, 2023Dorothea Ekoka Mbassi, Ghyslain Mombo-Ngoma, Jana Held, Dearie Glory Okwu, Wilfrid Ndzebe-Ndoumba, Laura Charlotte Kalkman, Franck Aurelien Ekoka Mbassi, Lais Pessanha de Carvalho, Juliana Inoue, Malik Azeez Akinosho, et al.binder molecule. Samples were assayed in duplicates along with no-template and positive controls on the LightCycler 480 Instrument II (Roche Applied Science). The crossing point (Cp) values were calculated with the second derivative maximum

(15) Identification and validation of a prognostic-related mutant gene DNAH5 for hepatocellular carcinomaFrontiers in ImmunologyOctober 25, 2023Zebing Song, Xiaodong Song, Hang Li, Zongbing Cheng, Zengyi Mo, Xuewei Yang(RT-PCR) was conducted, in accordance with the manufacturer’s instructions, by using an RT-PCR system (LightCycler® 480 Instrument II; Roche Holding, Basel, Switzerland). The primers for the glyceraldehyde 3-phosphate dehydrogenase (GAPDH)

(16) Tübingen model study: large-scale introduction of rapid antigen testing in the population and the viral dynamics of SARS-CoV-2Frontiers in Public HealthOctober 24, 2023Jule Ayran, Carsten Köhler, Le Thi Kieu Linh, Gisela Schneider, Srinivas Reddy Pallerla, Florian Battke, Lisa Federle, Peter Martus, Peter G. Kremsner, Thirumalaisamy P. VelavanRealStar® SARS-CoV-2 real-time PCR targeting the S gene (Altona Diagnostics, Hamburg, Germany) using a LightCycler® 480 Instrument II (Roche Diagnostics, Mannheim, Germany) as described previously (8). RT-qPCR was performed within a median

(17) Phospholipid isotope tracing reveals β-catenin-driven suppression of phosphatidylcholine metabolism in hepatocellular carcinomabioRxivOctober 21, 2023Chad VanSant-Webb, Hayden K. Low, Junko Kuramoto, Claire E. Stanley, Hantao Qiang, Audrey Su, Alexis N. Ross, Chad G. Cooper, James E. Cox, Scott A. Summers, et al.cDNA reactions and plated in duplicate. qPCR was performed using the LC480 PCR Lightcycler (Roche, 05015278001) using the “Mono Color Hydrolysis Probe/UPL probe” detection format. The temperature cycle consisted

(18) RING finger protein 13 protects against nonalcoholic steatohepatitis by targeting STING-relayed signaling pathwaysNature CommunicationsOctober 20, 2023Zhibin Lin, Peijun Yang, Yufeng Hu, Hao Xu, Juanli Duan, Fei He, Kefeng Dou, Lin Wangmeasured by ChamQ SYBR qPCR Master Mix (Q311-02, Vazyme; Nanjing, China), in a Real-Time PCR System (LightCycler 480 Instrument II, Roche; Basel, BS, Switzerland), following the manufacturer’s instructions. The mRNA abundance of the

(19) Heat shock protein 90 (Hsp90) inhibitor STA-9090 (Ganetespib) ameliorates inflammation in a mouse model of atopic dermatitisCell Stress and ChaperonesOctober 18, 2023Krzysztof Sitko, Michał Starke, Stefan TukajTranscription Kit (QIAGEN). The mRNA levels were assessed using QuantiNova SYBR Green PCR Kit (QIAGEN) in LightCycler® 480 Instrument II (Roche). Relative expression of filaggrin (FLG) was monitored. Here, using BestKeeper software, three

(20) Mode of action of antimicrobial agents albofungins in eradicating penicillin- and cephalosporin-resistant Vibrio parahaemolyticus biofilmMicrobiology SpectrumOctober 17, 2023Weiyi She, Aifang Cheng, Wenkang Ye, Ping Zeng, Hao Wang, Pei-Yuan Qiancarried out using LightCycler 480 SYBR Green I Master (Roche, Basel, Switzerland) on a Roche Diagnostics LightCycler 480 Instrument II Real-time PCR System machine. Subsequently, the glyceraldehyde-3-phosphate dehydrogenase gene was utilized

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4/2/2014The Standard for Comparing All Other PCR Instruments Robert LangEasy enough to train undergrads on, but powerful enough for a senior research scientist, the Roche LightCycler 480 is the gold standard I use to compare all other PCR instruments to for gene ... Read Review

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