LightCycler® 96 Instrument from Roche

Product Specs
ItemLightCycler^® 96 Instrument
CompanyRoche
Price Get Pricing
Catalog Number05815916001
QuantityEA
Wells96 Wells
ApplicationsRelative Quantification, Absolute Quantification, Melting Peak, Endpoint Genotyping, Primary applications include gene expression analysis, Gene detection, Mutation detection, Methylation analysis, miRNA research, 4-color multiplexing
TimeLess than an hour.
Temperature Range37 to 98 °C
SensitivitySensitivity of one copy
Excitation SourceNew Generation High-intensity and broad spectrum LED, 470 nm - 645 nm
DetectionCooled CCD camera; Up to 4 colors; Glass fiber optics
Dimensions(wXdXh) 40 x 40 x 53 cm (16 x 16 x 21 inches)
DisplayColor touch screen
Channels4

Features

Connectivity (USB, Network); Silver block

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Tumor-infiltrating platelets promote the growth of lung adenocarcinomaTranslational OncologyJanuary 1, 2024Xiaohui Li, Ming Li, Ziming Hu, Li Zhou, Meijuan Zheng, Defeng Jiao, Jingkun Qin, Binqing Fu, Xiaohu Zheng, Haiming Weithen performed using the SYBR Green Premix Pro-Taq HS qPCR Kit (Accurate biology, Cat#AG11701) on a LightCycler 96 instrument (Roche). Relative mRNA levels were calculated using the 2−ΔΔCt method and normalized to GAPDH housekeeping

(2) Early-life stress triggers long-lasting organismal resilience and longevity via tetraspaninbioRxiv : the preprint server for biologyDecember 17, 2023Jiang WI, De Belly H, Wang B, Wong A, Kim M, Oh F, DeGeorge J, Huang X, Guang S, Weiner OD, et al.by using ChamQ Universal SYBR qPCR Master Mix (Q711-02, Vazyme) on the Roche LightCycler96 (Roche, 05815916001) system. Ct values of tsp-1 were normalized to measurements of rps-23 (C. elegans) levels. Primer for

(3) Clinical Evaluation of Direct Reverse Transcription PCR for Detection of SARS-CoV-2 Compared to Conventional RT-PCR in Patients with Positive Rapid Antigen Test Results during Circulation of Emerging Viral VariantsDiagnostics (Basel)December 14, 2023Ming-Jr Jian, Chi-Sheng Chen, Hsing-Yi Chung, Chih-Kai Chang, Cherng-Lih Perng, Hung-Sheng Shangreference method. Residual viral transport medium (VTM) was analyzed by direct RT-PCR conducted on a LightCycler 96 instrument (Roche, Mannheim, Germany). We focused on the subjects who tested positive with an at-home RAT with

(4) Comparison between Sampling Techniques for Virological Molecular Analyses: Dolphin Morbillivirus and Herpesvirus Detection from FTA® Card and Frozen TissueVirusesDecember 13, 2023Haiyang Si, Claudia Maria Tucciarone, Mattia Cecchinato, Matteo Legnardi, Sandro Mazzariol, Cinzia Centelleghe(Thermofisher Scientific, Waltham, MA, USA) and PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™, Waltham, MA, USA) on LightCycler® 96 Instrument (Roche, Basil, Switzerland) to evaluate the presence of nucleic acids in the samples after the extraction. 3.

(5) Transcriptional Regulation of Methanol Dehydrogenases in the Methanotrophic Bacterium Methylococcus capsulatus Bath by Soluble and Insoluble LanthanidesMicrobes EnvironDecember 13, 2023Ruoyun Xie, Motoko Takashino, Kensuke Igarashi, Wataru Kitagawa, Souichiro Katogene expression analysis was performed by one-step real-time quantitative RT-PCR (qRT-PCR) using the LightCycler 96 Instrument (Roche Life Science) using RNA-direct SYBR Green Realtime PCR Master Mix (Toyobo) as previously described

(6) Alkaline phosphatase LapA regulates quorum sensing–mediated virulence and biofilm formation in Pseudomonas aeruginosa PAO1 under phosphate depletion stressMicrobiology SpectrumDecember 12, 2023Xiaojuan Tan, Xi Cheng, Jingjing Xiao, Qianqian Liu, Dongsheng Du, Minghui Li, Yang Sun, Jinwei Zhou, Guoping Zhu(Monad, China) according to the manufacturer’s instructions. These reactions were performed using the LightCycler 96 Instrument (Roche Diagnostics, USA) by applying the following cycle parameters: 95°C for 30 s, followed by 40 cycles

(7) New Insights into the TIFY Gene Family of Brassica napus and Its Involvement in the Regulation of Shoot BranchingInt J Mol SciDecember 4, 2023Yarong Li, Qian Zhang, Luman Wang, Xinfa Wang, Jiangwei Qiao, Hanzhong Wangmaster mix (71782420, LightCycler® 480 SYBR® Green 1 Master, Roche, Basel, Switzerland) on a Roche LightCycler® 96 instrument (Roche, Basel, Switzerland). The PCR reaction volume comprised 5 μL of SYBR Green I Master, 1 μL of

(8) Mitochondrial membrane potential acts as a retrograde signal to regulate cell cycle progressionLife Science AllianceDecember 1, 2023Choco Michael Gorospe, Gustavo Carvalho, Alicia Herrera Curbelo, Lisa Marchhart, Isabela C Mendes, Katarzyna Niedźwiecka, Paulina H Wanrooijprimers targeting COX1 or ACT1 (Table 2) and 10 μl 2x SyGreen Mix (PCR Biosystems), and were run on a LightCycler 96 instrument (Roche) using the following program: 95°C for 180 s, 45 cycles of (95°C for 10 s, 56°C for 10 s, and

(9) Individual heterogeneity in ixodid tick infestation and prevalence of Borrelia burgdorferi sensu lato in a northern community of small mammalian hostsOecologiaDecember 1, 2023Lars K. Lindsø, Jason L. Anders, Hildegunn Viljugrein, Anders Herland, Vetle M. Stigum, W. Ryan Easterday, Atle Mysterudcome from the genospecies B. afzelii (Mysterud et al. 2019a). The qPCRs were carried out on a Roche Lightcycler® 96 instrument. The qPCR was performed in 10 μl reactions composed of 2.0 μl of 5 × PCR Buffer (F. Hoffmann-La Roche

(10) Screening of an efficient cholesterol-lowering strain of Lactiplantibacillus plantarum 54–1 and investigation of its degradation molecular mechanismUltrasonics SonochemistryDecember 1, 2023Xiankang Fan, Nan Ling, Chunli Liu, Mingzhen Liu, Jue Xu, Tao Zhang, Xiaoqun Zeng, Zhen Wu, Daodong PanThe qPCR reaction for the differential genes was performed on a 20 μL reaction system using a Roche LightCycler® 96 instrument, following the method of Fan et al. with slight modifications [22]. 2.9 Statistical analysis All experiments

(11) Modeling of intramembranous ossification using human pluripotent stem cell-derived paraxial mesoderm derivativesRegenerative TherapyDecember 1, 2023Yuki Ikeda, Shoichiro Tani, Takeshi Moriishi, Aiko Kuroda, Yuki Matsuo, Naoya Saeki, Chizuko Inui-Yamamoto, Makoto Abe, Takashi Maeda, David W. Rowe, et al.Nanjing, China), 10 μM of specific primers, and cDNA. qPCR was performed with a LightCycler 480 system or LightCycler 96 Instrument (Roche, Basel, Switzerland) under the following conditions: 40 cycles of 95 °C for 10 s and 60 °C for

(12) SUV39H1 is a novel biomarker targeting oxidative phosphorylation in hepatitis B virus-associated hepatocellular carcinomaBMC CancerNovember 28, 2023Yanping Zhang, Wanwen Lao, Kaming Yang, Xinyi Kong, Yuetong Li, Xin Yu, Xumeng Wang, Yang Liu, Zhenlin Li, Yilin Deng, et al.Biology, China), and amplified with SYBR Green premix Pro Taq HS qPCR Kit (Accurate Biology, China) using LightCycler 96 instrument (Roche, Switzerland). The PCR primers used were listed in Table S1 [21]. The relative expression was

(13) Melatonin-Mediated Enhancement of Photosynthetic Capacity and Photoprotection Improves Salt Tolerance in WheatPlants (Basel)November 27, 2023Di Yan, Jiajie Wang, Zhenzong Lu, Rui Liu, Yue Hong, Baocai Su, Ye Wang, Zhen Peng, Chunxin Yu, Yuerong Gao, et al.information of TaActin1 (internal reference gene) and six randomly selected DEGs are shown in Table S1. LightCycler 96 Instrument (F. Hoffmann-La Roche Ltd., Indianapolis, IN, USA) was applied for qRT-PCR analysis. The samples used

(14) USP35 promotes HCC development by stabilizing ABHD17C and activating the PI3K/AKT signaling pathwayCell Death DiscoveryNovember 22, 2023Linpei Wang, Jiawei Wang, Xiaoqiu Ma, Guomin Ju, Chunfeng Shi, Wei Wang, Jian Wuof RNA was carried out using HiScript Reverse Transcriptase (Vazyme, China). qPCR was performed on a LightCycler 96 instrument (Roche, USA) using ChamQ qPCR Mix (Vazyme, China). At least 3 biological replicates were performed in

(15) Hierarchical contribution of Argonaute proteins to antiviral protectionJournal of Experimental BotanyNovember 21, 2023Márta Ludman, Gabriella Szalai, Tibor Janda, Károly FátyolGreen Master kit (Roche) according to the manufacturer’s instruction. Measurements were performed on a LightCycler 96 Instrument (Roche). The mRNA levels of AGO genes were determined using appropriate gene-specific primers. The measured

(16) Construction of a molecular regulatory network related to fat deposition by multi-tissue transcriptome sequencing of Jiaxian red cattleiScienceNovember 17, 2023Shuzhe Wang, Cuili Pan, Hui Sheng, Mengli Yang, Chaoyun Yang, Xue Feng, Chunli Hu, Yun Matranscription.64 Real-time quantitative PCR (qRT-PCR) was performed using the TaKaRa quantitative kit on a LightCycler® 96 instrument (Roche, Germany) to measure the expression levels of non-coding RNAs. All primers used are listed in

(17) Role of peptide–cell surface interactions in cosmetic peptide applicationFrontiers in PharmacologyNovember 13, 2023Bingwei He, Feifei Wang, Liping QuSYBR Green PCR Master Mix (Roche), 2 µL of cDNA template, and 0.4 µL of each primer (10 µM) using a LightCycler 96 instrument (Roche). The standard cycling conditions were 95°C for 10 min, followed by 40 cycles of 95°C for 30

(18) Assessment of the Genetic Characteristics of a Generation Born during a Long-Term Socioeconomic CrisisGenes (Basel)November 11, 2023Svetlana V. Mikhailova, Dinara E. Ivanoshchuk, Pavel S. Orlov, Ahmad Bairqdar, Maksim S. Anisimenko, Diana V. Denisovars12720071, rs806377, rs6557168, rs4311, rs4522666, and rs1800497 was conducted using real-time PCR on a LightCycler 96 instrument (Roche, Basel, Switzerland). Flanking oligonucleotides were selected in Primer-BLAST. TaqMan probes

(19) Regulatory Mechanism of Peroxisome Number Reduction Caused by FgPex4 and FgPex22-like Deletion in Fusarium graminearumJournal of FungiNovember 6, 2023Chunjie Liu, Zhuoyu Bi, Hao Xu, Renjie Zhang, Jiayi Wang, Yuancun Liang, Li Zhang, Jinfeng Yusystem, greatly simplifying the operation process and reducing the probability of contamination. A Lightcycler® 96 instrument (Roche Inc., Branchburg, NJ, USA) was used for DNA strand amplification, signal detection, and data

(20) Non-coding RNA-mediated endothelial-to-mesenchymal transition in human diabetic cardiomyopathy, potential regulation by DNA methylationCardiovascular DiabetologyNovember 3, 2023Eric Wang, Shali Chen, Honglin Wang, Tori Chen, Subrata Chakrabartitime reverse transcription quantitative polymerase chain reaction (RT-qPCR) was performed using the LightCycler 96 instrument (Roche). Each RT-qPCR reaction comprised 10 μL SYBR Advantage qPCR Premix (Clontech), 1 μL each of forward

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Analysis of Genetic Variations with High Resolution Melting
April 08, 2014
High Resolution Melting, HRM, is a post-PCR, closed-tube technique that can be used to analyze genetic variations. HRM allows you to rapidly perform single nucleotide polymorphism (SNP) or point mutational analysis, ... read more

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LightCycler^® 96 Instrument from Roche

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Roche

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Analysis of Genetic Variations with High Resolution Melting

LightCycler^® 96 Instrument from Roche

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