C6/lacZ7 from ATCC

Product Specs
ItemC6/lacZ7
CompanyATCC
Price $417.00Supplier Page View Company Product Page
Catalog NumberCRL-2303
SpeciesRattus norvegicus
TypeCell Biology
Cell Typeglial cell
Validated Species ReactivityRat (Rt)
DiseaseGlioma
TissueBrain
ApplicationsC6/lacZ7 is a subclone of the C6/lacZ cell line (ATCC CRL-2199) that was established in 1989 from the N-nitrosomethylurea-induced glial rat, outbred strain, C6 cell line.The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production.The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide.The contrast between stained cells and background facilitates image analysis.The depositor has confirmed that the C6/lacZ7 subclone grows in rat brain, and that the lacZ marker can be detected by histochemistry for b-gal.This is one of few models that permit quantitative analysis of microscopic tumor in the brain.The beta-gal expression is less stable than for 9L/lacZ. Cells should be used soon after thawing or re-cloned.Methods for the use of C6/lacZ may be found in Cancer Res

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Post freeze viability ≥ 50%

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ATCC

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Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

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Website: http://www.atcc.org

(1) Effects of HO-1 on the Integrity of Retinal Pigment Epithelial Cells in Regulating Axial Elongation in Experimental MyopiaInvest Ophthalmol Vis SciApril 17, 2026He-Yan Li, Yu-Hang Yang, Li Dong, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Jiao-Yue Dong, Chu-Yao Yu, Shan-Shan Wang, Wen-Bin WeiOxidative Stress Induction ARPE19 cells were purchased from the American Type Culture Collection (CRL-2303; ATCC, Manassas, VA, USA) and were cultured in a 5% CO2 incubator at 37°C. The culture medium was Gibco

(2) AAV-mediated multiple gene therapy combining VEGFA-targeting miR-agshRNAs and PEDF for the suppression of choroidal neovascularizationMolecular Therapy Nucleic AcidsMarch 12, 2026Bjørn K. Fabian-Jessing, Anne Louise Askou, Thomas Stax Jakobsen, Rikke Lentz Adsersen, Anna Bøgh Lindholm, Anne Kirstine Køllner Bjerre, Sidsel Alsing, Toke Bek, Lars Aagaard, Thomas J. Corydonmg/mL of streptomycin (both from Gibco, Thermo Fischer Scientific, Odense, Denmark). ARPE-19 cells (CRL-2303; ATCC) were kept in 50/50% DMEM/F12 medium supplemented with 5% FCS (Sigma), 100 U/mL of penicillin,

(3) Murine Cytomegalovirus and Human Cytomegalovirus Differ in Pyroptosis Induction in Different Cell Types During Productive ReplicationVirusesAugust 12, 2025Jessica J. Carter, Daniel H. Schneider, Arshaan M. Hisamuddin, Richard D. Dixmaintained according to ATCC recommendations. Human retinal pigmented epithelial cells (ARPE-19) (No. CRL-2303) obtained from ATCC were grown in DMEM: F12 containing 10% FBS, 1% penicillin/streptomycin, 0.1 mg/mL

(4) Lack of strong innate immune reactivity renders macrophages alone unable to control productive Varicella-Zoster Virus infection in an isogenic human iPSC-derived neuronal co-culture modelFrontiers in immunologyJanuary 1, 2023Van Breedam E, Buyle-Huybrecht T, Govaerts J, Meysman P, Bours A, Boeren M, Di Stefano J, Caers T, De Reu H, Dirkx L, et al.VZV-eGFP/ORF23 strain (27), propagated in the human retinal pigment epithelial cell line ARPE-19 (ATCC, CRL-2303), as described in our earlier work (9). VZV-eGFP/ORF23-infected ARPE-19 cells were cultured in DMEM/F12

(5) Dependence of Retinal Pigment Epithelium Integrity on the NRF2–Heme Oxygenase-1 AxisInvestigative Opthalmology & Visual ScienceAugust 29, 2022Yida Jiang, Li-Juan Duan, Jingbo Pi, Yun-Zheng Le, Guo-Hua Fongsubject. Methods Chemical Treatment of ARPE19 Cells ARPE19 cells were purchased from the American Type Culture Collection (CRL-2303; ATCC, Manassas, VA, USA) and were cultured in a 5% CO2 incubator at 37°C. The culture medium was Gibco

(6) Rat and Mouse Brain Tumor Models for Experimental Neuro-Oncology ResearchJournal of Neuropathology & Experimental NeurologyApril 27, 2022Upasana Sahu, Rolf F Barth, Yoshihiro Otani, Ryan McCormack, Balveen Kauraddition, 2 C6 sublines with the LacZ reporter gene are currently available from the ATCC (C6/lacZ 7 [CRL-2303] and C6/lacZ [CRL-2199]). Genomics As summarized in Table 2, genes expressed by the C6 glioma relevant

(7) MBP-11901 Inhibits Tumor Growth of Hepatocellular Carcinoma through Multitargeted Inhibition of Receptor Tyrosine KinasesCancersApril 14, 2022Park HJ, Choi G, Ha S, Kim Y, Choi MJ, Kim M, Islam MK, Chang Y, Kwon TJ, Kim D, et al.adenocarcinoma cells (MCF-7, ATCC® HTB-22™ and MDA-MB-231, ATCC® HTB-26™), rattus brain glioma cells (C6, ATCC® CRL-2303™), and human renal carcinoma cells (Caki-2, ATCC® HTB-47™) were cultured in Dulbecco’s modified Eagle’s medium (DMEM

(8) β-Cleavage of the prion protein in the human eye: Implications for the spread of infectious prions and human ocular disordersExperimental eye researchNovember 1, 2021Chaudhary S, Ashok A, Wise AS, Rana NA, Kritikos AE, Lindner E, Singh N.obtained from Santa Cruz Biotechnology, Inc, USA. 2.4. Cell lines and experimental design ARPE-19 (CRL-2303™) and BE(2)-M17 (CRL-2267™) cells were obtained from ATCC, and primary cultures of TM cells were established

(9) Synthesis, Characterization, and Anticancer Activity of Benzothiazole Aniline Derivatives and Their Platinum (II) Complexes as New Chemotherapy AgentsPharmaceuticals (Basel)August 23, 2021Md. Kamrul Islam et al.(MCF-7, ATCC® HTB-22™), hepatocellular carcinoma (HepG2, ATCC® HB-8065™), Rattus brain glioma (C6, ATCC ® CRL-2303™), and human renal carcinoma (Caki-2, ATCC® HTB-47™) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM

(10) The Immunomodulatory Potential of Copper and Silver Based Self-Assembled Metal Organic Biohybrids Nanomaterials in Cancer TheranosticsFrontiers in ChemistryJanuary 27, 2021Neela Prajapati, Anik Karan, Elnaz Khezerlou, Mark A. DeCostersynthesized CuHARS and AgCysNPs were compared as shown in Figures 1A,B, respectively. Cell Culture CRL2303(ATCC) glioma cells were cultured in a complete growth medium containing DMEM (ATCC), 10% Fetal Bovine Serum

(11) Self-Assembled Metal–Organic Biohybrids (MOBs) Using Copper and Silver for Cell StudiesNanomaterials (Basel)September 8, 2019Neha Karekar, Anik Karan, Elnaz Khezerlou, Neela Prajapati, Chelsea D. Pernici, Teresa A. Murray, Mark A. DeCosteraddition of nanomaterials on top of them [1,12,13]. 2. Materials and Methods 2.1. Cell Culture (1) CRL-2303 glioma cells were maintained, as suggested by the vendor (ATCC, Manassas, VA, USA), in complete growth

(12) Oxidative stress induces ferroptotic cell death in retinal pigment epithelial cellsExperimental eye researchApril 1, 2019Totsuka K, Ueta T, Uchida T, Roggia MF, Nakagawa S, Vavvas DG, Honjo M, Aihara M.Cell culture Author Manuscript Author Manuscript The human retinal pigment epithelial (RPE) cell line ARPE-19 (CRL-2303; ATCC, Manassas, VA, USA) cells were cultured in Dulbecco’s modified Eagle’s medium with nutrient mixture

(13) Integration of a Copper-Containing Biohybrid (CuHARS) with Cellulose for Subsequent Degradation and Biomedical ControlInternational Journal of Environmental Research and Public HealthApril 25, 2018Anik Karan, Margarita Darder, Urna Kansakar, Zach Norcross, Mark A. DeCostermedia evaluated contained complete growth components including serum. The evaluated media included: (1) CRL-2303 (brain tumor, glioma) cell media, prepared as previously described [24] and as suggested by the vendor

(14) Sublethal Photothermal Stimulation with a Micropulse Laser Induces Heat Shock Protein Expression in ARPE-19 CellsJournal of OphthalmologyNovember 30, 2015Keiji Inagaki, Takuya Shuo, Kanae Katakura, Nobuyuki Ebihara, Akira Murakami, Kishiko OhkoshiMaterials and Methods 2.1. Cell Culture. The ARPE-19 immortal human RPE cell line [27] at passage 19 (ATCC CRL-2303, Lot number: 60279299) was purchased from American Type Culture Collection (Manassas, VA, USA), and

(15) Rat brain tumor models in experimental neuro-oncology: the C6, 9L, T9, RG2, F98, BT4C, RT-2 and CNS-1 gliomasJournal of Neuro-OncologySeptember 1, 2009Rolf F. Barth, Balveen Kaur2010 September 1. Barth and Kaur Page 4 NIH-PA Author Manuscript available through the ATCC (# CRL-2303). However, it must be noted that the β-galactosidase marker protein itself can serve as a tumor antigen,

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C6/lacZ7 from ATCC

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