DI TNC1 from ATCC

Product Specs
ItemDI TNC1
CompanyATCC
Price $338.00Supplier Page View Company Product Page
Catalog NumberCRL-2005
SpeciesRattus norvegicus
TypeCell Biology
Cell Typeastrocyte also type II
Validated Species ReactivityRat (Rt)
Ageneonate
DiseaseNormal
TissueDiencephalon, Brain

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) A triple-action PROTAC for wild-type p53 cancer therapyCell Reports MedicineDecember 16, 2025Gregory H. Bird, Utsarga Adhikary, Michael J. Schmidt, Marina Godes, Bethany Tesar, Christina M. Camara, Joao A. Paulo, Julia F. Vidlak, Thomas M. DeAngelo, Marilyn Marquez, et al.WT,ATCC,Cat# CCL-247 HCT116 p53−/−,B. Vogelstein Laboratory,N/A C6,ATCC,Cat# CCL-107 DI-TNC1,ATCC,Cat# CRL-2005 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(2) Enhancing Antioxidants Performance of Ceria Nanoparticles in Biological Environment via Surface Engineering with o-Quinone FunctionalitiesAntioxidants (Basel)July 25, 2025Pierluigi Lasala, Tiziana Latronico, Umberto Mattia, Rosa Maria Matteucci, Antonella Milella, Matteo Grattieri, Grazia Maria Liuzzi, Giuseppe Petrosillo, Annamaria Panniello, Nicoletta Depalo, et al.purchased from Sigma-Aldrich (St. Louis, MO, USA), if not differently stated. The DI-TNC1 cell line (ATCC CRL-2005) was acquired and authenticated from the ATCC (www.lgcstandards-atcc.org, accessed on 21 November 2024).

(3) Tetramethylpyrazine attenuates the blood-brain barrier damage against ischemic stroke by targeting endothelin-1/Akt pathway in astrocytesFront PharmacolMay 16, 2025Minzhen Deng, Yuefang Cai, Yu Wang, Dafeng Hu, Yan Li, Zhenqiu Ning, Chengyi Wang, Sookja Kim Chung, Yan Huang, Jingbo Sun, et al.(NC) and transfection kit were provided by Ribobio Corporation (Guangzhou, China). Astrocytes (ATCC, CRL-2005) were cultured in antibiotic-free medium and incubated with ET-1 siRNA or NC for 24 h, then OGD 6 h

(4) Increased Antioxidant Performance of Lignin by Biodegradation Obtained from an Extract of the Mushroom Pleurotus eryngiiMoleculesNovember 26, 2024Tania Petraglia, Tiziana Latronico, Antonietta Pepe, Aniello Crescenzi, Grazia Maria Liuzzi, Rocco Rossano[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay as reported by Latronico et al. [38]. The DI-TNC1 cell line (ATCC CRL-2005) was acquired and authenticated from the ATCC (www.lgcstandards-atcc.org, (accessed on 21 November 2024)).

(5) Methadone directly impairs central nervous system cells in vitroScientific ReportsJuly 23, 2024Cristian De Gregorio, Javiera Gallardo, Pablo Berríos-Cárcamo, Álex Handy, Daniela Santapau, Antonia González-Madrid, Marcelo Ezquer, Paola Morales, Alejandro Luarte, Daniela Corvalán, et al.activation. HMC3 (inmortalized human microglia, CRL-3304, ATCC) and DI TNC1 (inmortalized P1 rat astrocytes, CRL-2005, ATCC) cell lines were seeded at a density of 40.000 cells per well in 6-well plates to avoid overgrow

(6) Effect of Bromfenac on Reducing Neuroinflammation in an Ischemia–Reperfusion Glaucoma ModelCellsJune 17, 2024Si-Eun Oh, Jie-Hyun Kim, Chan-Kee Park, Hae-Young Lopilly Park(Catalog No. CL-0493, Houston, TX, USA) and the DI TNC1 astrocyte cell line from ATCC (Catalog No. CRL 2005, Seoul, Republic of Korea) were cultured in DMEM (Gibco, Grand Island, NY, USA) supplemented with 10%

(7) Polar lipids modify Alzheimer's Disease pathology by reducing astrocyte pro-inflammatory signaling through platelet-activating factor receptor (PTAFR) modulationLipids in health and diseaseApril 20, 2024Hans S, Stanton JE, Sauer AK, Shiels K, Saha SK, Lordan R, Tsoupras A, Zabetakis I, Grabrucker AM.is more potent in inhibiting the PTFAR pathway. Materials and methods Materials DI TNC1 (ATCC® CRL-2005™) rat astrocyte cells were used as the cell culture model. Lipid extraction reagents (hexane, ethanol,

(8) Testosterone Inhibits Secretion of the Pro-Inflammatory Chemokine CXCL1 from AstrocytesCurr Issues Mol BiolMarch 6, 2024Malgorzata Turniak-Kusy, Maciej Studzian, Piotr Szpakowski, Piotr Kuchta, Kaja Smietanka, Claudia Mattern, Lukasz Pulaski, Bartosz Bieleckiand Methods 2.1. Cell Culture Type 1 astrocyte cell line DI TNC1 was purchased from ATCC (cat. no. CRL-2005). The cells were cultured at 37 °C in a 5% CO2 humidified atmosphere in high glucose Dulbecco’s modified

(9) Antioxidant Activity of Polysaccharides from the Edible Mushroom Pleurotus eryngiiMoleculesFebruary 26, 2023Tania Petraglia, Tiziana Latronico, Angela Fanigliulo, Aniello Crescenzi, Grazia Maria Liuzzi, Rocco RossanoItaly), Bio-Rad Laboratories (Segrate, Italy) and GE Healthcare (Uppsala, Sweden). The DI-TNC1 (ATCC CRL-2005) cell line, established from primary cultures of type 1 astrocytes from brain diencephalon tissue of

(10) GABAergic Regulation of Astroglial Gliotransmission through Cx43 HemichannelsInt J Mol SciNovember 7, 2022Ivanka JimÃ©nez-Dinamarca, Rachel Reyes-Lizana, Yordan Lemunao-Inostroza, Kevin CÃ¡rdenas, Raimundo Castro-Lazo, Francisca PeÃ±a, Claudia M. Lucero, Juan Prieto-Villalobos, Mauricio Antonio Retamal, Juan AndrÃ©s Orellana, et al.Materials and Methods 4.1. DI TNC1 Astroglial Cell Culture The rat astrocyte cell line DI TNC1 (ATCC CRL-2005) was cultured at 37 °C, and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 5%

(11) An Outside-In Switch in Integrin Signaling Caused by Chemical and Mechanical Signals in Reactive AstrocytesFrontiers in Cell and Developmental BiologyAugust 23, 2021Leonardo A. Pérez, Aysha Rashid, J. Dale Combs, Pascal Schneider, Andrés Rodríguez, Khalid Salaita, Lisette Leytonrepair upon brain damage. Materials and Methods Cell Line The rat astrocytic cell line DITNC1 (ATCC CRL-2005) was obtained from P. Magistretti (University of Lausanne, Switzerland), maintained in RPMI medium 1640

(12) Design and In Vitro Study of a Dual Drug-Loaded Delivery System Produced by Electrospinning for the Treatment of Acute Injuries of the Central Nervous SystemPharmaceuticsJune 8, 2021Luisa Stella Dolci et al.line RAW 264.7 (ATCC® TIB-71™) was selected for efficacy studies, whereas rat astrocyte DITNC1 (ATCC® CRL-2005™) was used for toxicity studies. Cells were grown in DMEM high glucose medium (Thermo Fisher Scientific,

(13) Expression Analysis of Zinc Transporters in Nervous Tissue Cells Reveals Neuronal and Synaptic Localization of ZIP4International journal of molecular sciencesApril 26, 2021De Benedictis CA, Haffke C, Hagmeyer S, Sauer AK, Grabrucker AM.at 37 ◦ C and 5% CO2 for 14 days. 4.4. Cultivation of Cell Lines DI TNC1 (ATCC, Manassas, VA, USA: CRL-2005) rat astrocytes were seeded (50,000 cells/mL) on poly-L-lysine (0.1 mg/mL)-coated 10 cm Petri dish and

(14) Hypertonic saline mediates the NLRP3/IL-1β signaling axis in microglia to alleviate ischemic blood-brain barrier permeability by downregulating astrocyte-derived VEGF in ratsCNS neuroscience & therapeuticsOctober 1, 2020Wang QS, Ding HG, Chen SL, Liu XQ, Deng YY, Jiang WQ, Li Y, Huang LQ, Han YL, Wen MY, et al.the culture medium containing 10% FBS. 2.3 TNC1 astrocyte culture and groups DI TNC1 cells (ATCC, CRL‐2005™) were maintained in 75‐cm2 culture flasks with DMEM‐F12 supplemented with 10% FBS. The cultures were

(15) Syndecan-4/PAR-3 signaling regulates focal adhesion dynamics in mesenchymal cellsCell communication and signaling : CCSAugust 18, 2020Valdivia A, Cárdenas A, Brenet M, Maldonado H, Kong M, Díaz J, Burridge K, Schneider P, San Martín A, García-Mata R, et al.role in polarized cell migration. Methods Cell culture The rat astrocytic cell line DI TNC1 (ATCC CRL-2005) was maintained in RPMI-1640 medium (GIBCO Life Technologies) as previously described [17]. CAD cells

(16) Brivaracetam attenuates pain behaviors in a murine model of neuropathic painMolecular painJanuary 1, 2019Tsymbalyuk S, Smith M, Gore C, Tsymbalyuk O, Ivanova S, Sansur C, Gerzanich V, Simard JM.from mouse brain, from an immortalized rat Molecular Pain astrocyte cell line (DI TNC1; catalogue #CRL-2005; ATCC, Gaithersburg, MD, USA) and from an immortalized human T-lymphocyte cell line (TIB-152; ATCC), using

(17) Intracellular Ca2+ Increases and Connexin 43 Hemichannel Opening Are Necessary but Not Sufficient for Thy-1-Induced Astrocyte MigrationInternational Journal of Molecular SciencesJuly 26, 2018Raúl Lagos-Cabré, Marianne Brenet, Jorge Díaz, Ramón D. Pérez, Leonardo A. Pérez, Rodrigo Herrera-Molina, Andrew F. G. Quest, Lisette LeytonMaterials and Methods 4.1. Cell Line and Primary Cultures The rat astrocytic cell line DITNC1 (ATCC CRL-2005) was maintained in RPMI medium 1640 Int. J. Mol. Sci. 2018, 19, 2179 10 of 16 We have previously

(18) The Effects of Synthesized Rhenium Acetylsalicylate Compounds on Human Astrocytoma Cell LinesJournal of Cancer Science & TherapyMay 1, 2018Hirendra N. Banerjee et al.corresponding RAC compounds. Cell Culture Human astrocytoma (ATCC # HTB-12), rat astrocytes (ATCC # CRL-2005) cell lines were obtained (American Type Culture Collection, ATCC, Manassas, VA) D54 human Glioblastoma

(19) Development of a Platform for Studying 3D Astrocyte Mechanobiology: Compression of Astrocytes in Collagen GelsAnnals of Biomedical EngineeringFebruary 1, 2018John J. E. Mulvihill, Julia Raykin, Eric J. Snider, Lisa A. Schildmeyer, Irsham Zaman, Manu O. Platt, Daniel J. Kelly, C. Ross Ethierreactivity markers, and protease activity. 81 Materials and Methods 82 Cell Sourcing 83 DI TNC1 cells (CRL 2005, ATCC), an immortalized cell line derived from primary cultures 84 of type 1 astrocytes taken from

(20) Phytochemicals and botanical extracts regulate NF-κB and Nrf2/ARE reporter activities in DI TNC1 astrocytesNeurochemistry InternationalJuly 1, 2016Deepa Ajit et al.rat brain diencephalon with the polyomavirus middle T-antigen, and this cell line was purchased from ATCC (CRL-2005, Rockville, MD). DI TNC1 cells were cultured in DMEM (high glucose), 10% (v/v) FBS, 100 units/mL penicillin

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