Random Primer Mix from New England Biolabs

Product Specs
ItemRandom Primer Mix
CompanyNew England Biolabs
Price $109.00 Inquire
Catalog NumberS1330S
ApplicationsDNA Analysis
Nucleic Acid TypeRandom Primers
TypePrimer
Temperature-20 C
Quantity100 µl

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous systemBrain Struct FunctApril 4, 2026Lukáš Malčický, Ján Košuth, Daniel Barčák, Jarmila Zrubáková, Marie Vancová, Zuzana Daxnerová, Juraj Ševc, Anna Alexovič Matiašovárandom hexamer primers and oligoT primers (random primer mix, New England Biolabs, Ipswich, MA, USA, #S1330S) using RevertAid Reverse Transcriptase (Thermo Scientific, Vilnius, Lithuania, #EP0442) following the

(2) Comparative Analysis of Prenatal Stress Models: Placental and Neurodevelopmental Outcomes in MiceYale J Biol MedMarch 31, 2026Mia Dukle, Faith M. Fairbairn, Sara V. Maurer, Madelyn M. Grueter, Ahmed I. Baig, Rachel Telles, Hanna E. Stevenswas synthesized (New England Biolabs AMV Reverse Transcriptase; NEB M0227L, Random Primer Mix; NEB S1330S). Ventral forebrain GABAergic system development genes [8,47] and placental immune genes similar to

(3) Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signalingPLoS GenetMarch 5, 2026Valentina Botero, Jenifer Barrios, Anneke Knauss, Greta Dahlen, Ethan Rosendahl, Kenneth J. Colodner, Seth M. TomchikMix (New England Biolabs, catalog #E3025L) with the Random Primer Mix (New England Biolabs, catalog #S1330S). Nf1 gene expression was quantified in fly head samples using Luna Universal qPCR Master Mix (New England Biolabs

(4) Dual promoter–enhancer activities reflect a unified regulatory logicNature CommunicationsJanuary 30, 2026Mauricio I. Paramo, Alden King-Yung Leung, Sagar R. Shah, Junke Zhang, Nathaniel D. Tippens, Jin Liang, Li Yao, Yiyang Jin, Xiuqi Pan, Abdullah Ozer, et al.DNase-treated RNA using LunaScript RT SuperMix Kit (E3010; New England Biolabs) and Random Primer Mix (S1330S; New England Biolabs). Parallel no-RT controls were performed to assess plasmid DNA contamination. cDNA

(5) Characterization and quantitation of baculoviral DNA in rAAV vectors produced in Sf9 cellsMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Xushan Wang, Andrew Pla, Vedud Purde, Sabine Wenzel, Priyam Raut, Diogo de Oliveira Pessoa, Jorge F. Haller, Stuart Nelson, Beverly A. Heinz, Sarah M. Richerreaction consisted of 600 ng extracted AAV DNA diluted in water to 28 μL, 5 μL random primer mix (NEB, S1330S), 10 μL dNTP solution mix (NEB, N0447S), 2 μL DNA Polymerase I, Large (Klenow) Fragment (NEB, M0212S),

(6) Chronic gut inflammation differentially modulates mitochondrial and antioxidant transcriptional programs in limbic brain structuresScientific ReportsDecember 10, 2025C E Matisz, V. Lapointe, K. Beekman, T. Haight, R. Das, J. Medlicott, R J Sutherland, A. Zovoilis, AJ. Gruber18090010, Invitrogen) by the following method: 100ng of total RNA was mixed with 100ng Random Primer Mix (S1330S, NEB), and 1 uL of 10mM dNTP mix (CAT N0447S, NEB). The mixture was incubated for 5 min at 65 °C and

(7) Histone Deacetylase Inhibitors orchestrate epigenetic signalling and alter the nucleoporins and nuclear envelope in cervical cancerTranslational OncologyNovember 1, 2025Harsha Rani, Shabir Ahmad Ganai, Vijayalakshmi Mahadevanremove the traces of contaminating DNA. cDNA synthesis was performed using random hexamer primers (NEB #S1330S) and M-MuLV Reverse Transcriptase (NEB M0253S) as per manufacturer instructions. Real-time-PCR analysis

(8) Whole genome sequencing and phylogenetic classification accelerate the implementation of respiratory syncytial virus genomic surveillance in Canada: a pilot studyMicrobiology SpectrumOctober 7, 2025Ruimin Gao, Cody Buchanan, Kerry Dust, Paul Van Caeseele, Henry Wong, Calvin Sjaarda, Prameet M. Sheth, Agatha N. Jassem, Jessica Minion, Nathalie Bastienused to synthesize cDNA from 5 µL of RNA in conjunction with 0.5 µL of 60 µM random hexamers (cat. no. S1330S, New England Biolabs [NEB]) in a final reaction volume of 10 µL. The reverse transcription reaction

(9) Androgen receptor antagonist flutamide modulates estrogen receptor alpha expression in distinct regions of the hypospadiac rat penisFront Endocrinol (Lausanne)September 12, 2025Emilie Elmelund, Monica K. Draskau, Marie Berg, Ida W. Strand, Jay R. Black, Marta Axelstad, Andrew J. Pask, Terje Svingenfrom 350 ng total RNA using the Omniscript RT kit (cat # 205113, Qiagen) and Random Primer Mix (cat # S1330S, New England Biolabs, Ipswich, MA, USA). For RT-qPCR, 3 µL 1:20 diluted cDNA was added to TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG

(10) Unbiased and comprehensive identification of virus-derived circular RNAs in a large range of viral species and familiesPLOS PathogensSeptember 11, 2025Alexis S. Chasseur, Maxime Bellefroid, Mathilde Galais, Meijiao Gong, Pierre Lombard, Sarah Mathieu, Amandine Pecquet, Estelle Plant, Camille Ponsard, Laure Vreux, et al.transcription was performed using the SuperScript IV kit (18090010 – Invitrogen) with random (250 nM; S1330S – New England Biolabs) or specific (100nM; Eurogentec) primers following the manufacturer’s recommendations.

(11) APOBEC3B Promotes SARS-CoV-2 Through Activation of PKR/eIF2⍺ and AMPD2 DysregulationVirusesAugust 28, 2025Benjamin Fixman, Lavanya Manjunath, Philip Sell, Shanshan Wang, Tamara Margaryan, Connor Qiu, Hanjing Yang, Rémi Buisson, Xiaojiang S. Chencontaining 1 µL of 100µM gene-specific reverse primer or 2 µL of 100µM random hexamer primers (NEB #S1330S (Ipswich, MA, USA)), 4 µL 1x Protoscript II buffer, 1 µL of 10 mM dNTP, 1 µL of 0.1 M DTT, 8U RNase

(12) Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signalingbioRxivJuly 31, 2025Valentina Botero, Jenifer Barrios, Anneke Knauss, Ethan Rosendahl, Kenneth J. Colodner, Seth M. TomchikMix (New England Biolabs, catalog #E3025L) with the Random Primer Mix (New England Biolabs, catalog #S1330S). Nf1 gene expression was quantified in fly head samples using Luna Universal qPCR Master Mix (New England Biolabs

(13) The contribution of de novo coding mutations to meningomyeloceleNatureMay 8, 2025Yoo-Jin Jiny Ha, Ashna Nisal, Isaac Tang, Chanjae Lee, Ishani Jhamb, Cassidy Wallace, Robyn Howarth, Sarah Schroeder, Keng Ioi Vong, Naomi Meave, et al.were synthesized using M-MLV reverse transcriptase (Invitrogen, 28025013) and random primers (NEB, S1330S). cDNAs were amplified by Taq polymerase (NEB, M02735) or Phusion High-Fidelity DNA polymerase (NEB,

(14) Viperin expression leads to downregulation of mitochondrial genes through misincorporation of ddhCTP by mitochondrial RNA polymeraseJournal of Biological ChemistryApril 1, 2025Srijoni Majhi, Pronay Roy, Minshik Jo, Jiying Liu, Rebecca Hurto, Lydia Freddolino, E. Neil G. Marshtranscribed using the ProtoScript II Reverse Transcriptase (M0368 L, NEB) using Random Primer Mix (S1330S, NEB) according to the manufacturer’s protocol. RT-qPCR was performed using the iTaq Universal SYBR Green Supermix

(15) Quorum sensing regulators and non-ribosomal peptide synthetases govern antibacterial secretions in Xenorhabdus szentirmaiiFront MicrobiolMarch 12, 2025Ritisha Dey, Domonique Olivia Valle, Abhijit Chakraborty, Kimberly A. Mayer, Jagadeesh Kumar Uppala, Anish Chakraborty, Shama Mirza, Troy Skwor, Steven Forst, Madhusudan Dey(Invitrogen, United States). Using 2 μg of total RNA, cDNA was prepared by random primers (NEB, USA, cat # S1330S) and NEB cDNA synthesis kit (NEB, USA, cat # E6300S). cDNA was then amplified by gene-specific primers

(16) Chemical evolution of ASO-like DNAzymes for effective and extended gene silencing in cellsNucleic Acids ResMarch 4, 2025Yingyu Liu, Sheyu Zhang, Meiqi Zhang, Xin Liu, Yashu Wu, Qin Wu, John C Chaput, Yajun Wang10-μl reverse transcription-template switching (RT-TS) reaction using a random primer mix (NEB, Cat# S1330S) and 0.35 μM of TSO. The reaction was incubated at 42°C for 90 min. The RT-TS reaction was diluted two-fold

(17) Identification and clinical implications of endogenous retrovirus elements suppressed by SETDB1 in hepatocellular carcinomaJHEP ReportsMarch 1, 2025Yosuke Igarashi, Yoshimitsu Akiyama, Shu Shimada, Shuichi Watanabe, Megumi Hatano, Keita Kodera, Kohei Okazaki, Yoshiaki Tanji, Shu Tsukihara, Tomohiko Taniai, et al.(Thermo Fisher Scientific) to analyze gene expression. For ERV RNA extraction from cell pellets, Random Primer Mix (S1330S, New England Biolabs, Japan) was used. Quantitative reverse transcription-PCR (qRT-PCR) was performed

(18) A Cell Autonomous Free fatty acid receptor 4 - ChemR23 Signaling Cascade Protects Cardiac Myocytes from Ischemic InjurybioRxivJanuary 6, 2025Sara J. Puccini, Chastity L. Healy, Brian A. Harsch, Ahmed R. Ahmed, Gregory C. Shearer, Timothy D. O’ConnellOligo(dt) Primer (ThermoFisher, Waltham, MA, Cat. #18418012), 60 μM Random Hexamer Primer (NEB, Cat. #S1330S), and Nuclease Free Water up to a final volume of 16 μL. The tubes were heated to 65°C for 10 minutes

(19) The skin microbiome stratifies patients with cutaneous T cell lymphoma and determines event-free survivalnpj Biofilms and MicrobiomesAugust 29, 2024Philipp Licht, Nazzareno Dominelli, Johannes Kleemann, Stefan Pastore, Elena-Sophia Müller, Maximilian Haist, Kim Sophie Hartmann, Henner Stege, Matthias Bros, Markus Meissner, et al.strand cDNA was carried out with M-MuLV Reverse Transcriptase (M0253L) using both Random Hexamer Primer (S1330S) and Oligo(dT) Primer (S1316S) (all from New England Biolabs, USA) at a total reaction volume of 20

(20) Loss of p53 epigenetically modulates epithelial to mesenchymal transition in colorectal cancerTranslational OncologyMay 1, 2024Shreya Sharma, Harsha Rani, Yeshwanth Mahesh, Mohit Kumar Jolly, Jagannath Dixit, Vijayalakshmi Mahadevanremove the traces of contaminating DNA. cDNA synthesis was performed using random hexamer primers (NEB #S1330S) and M-MuLV Reverse Transcriptase (NEB M0253S) as per manufacturer instructions. Real-time-PCR analysis

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