PvuII from New England Biolabs

Product Specs
ItemPvuII
CompanyNew England Biolabs
Price $273.00 Inquire
Catalog NumberR0151M
Quantity25000 units
Concentration50,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

5'-CAG/CTG-3'

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Deletion of Pax1 scoliosis-associated regulatory elements leads to a female-biased tail abnormalityCell ReportsMarch 26, 2024Aki Ushiki, Rory R. Sheng, Yichi Zhang, Jingjing Zhao, Mai Nobuhara, Elizabeth Murray, Xin Ruan, Jonathan J. Rios, Carol A. Wise, Nadav AhituvSouthern blot analyses, genomic DNA was treated with BglIl or PvuII (New England Biolabs; R0144 or R0151) and fractionated by agarose gel electrophoreses. Following capillary transfer onto nylon membranes,

(2) Deletion of Pax1 scoliosis-associated regulatory elements leads to a female-biased tail abnormalitybioRxivApril 18, 2023Aki Ushiki, Rory R. Sheng, Yichi Zhang, Jingjing Zhao, Mai Nobuhara, Elizabeth Murray, Xin Ruan, Jonathan J. Rios, Carol A. Wise, Nadav AhituvSouthern blot analyses, genomic DNA was treated with BglIl or PvuII (New England Biolabs; R0144 or R0151) and fractionated by agarose gel electrophoreses. Following capillary transfer onto nylon membranes,

(3) Increased fidelity of protein synthesis extends lifespanCell metabolismNovember 2, 2021Martinez-Miguel VE, Lujan C, Espie-Caullet T, Martinez-Martinez D, Moore S, Backes C, Gonzalez S, Galimov ER, Brown AEX, Halic M, et al.TGCTTGTCTGGAAAAAGATT and Taq DNA polymerase (NEB; M0273) was used for PCR. PvuII restriction (NEB; R0151) was performed after PCR, directly in a Taq PCR mixture, without prior DNA purification. The PCR product

(4) High-resolution mapping of a standardized transcriptional reporter reveals positions of high and low transcription in the Escherichia coli chromosomeCell SystemsMarch 1, 2019Scott A. Scholz, Rucheng Diao, Michael B. Wolfe, Elayne M. Fivenson, Xiaoxia Nina Lin, Peter L. Freddolinothe manufacturer’s instructions. In brief, barcoded pSAS31 plasmid was digested with PvuII (NEB Cat#R0151) for one hour at 37°C and fragments were separated on a 0.8% agarose gel. The band corresponding to

(5) Initiation and maintenance of pluripotency gene expression in the absence of cohesinGenes & DevelopmentJanuary 1, 2015Thais Lavagnolli, Preksha Gupta, Eva Hörmanseder, Hegias Mira-Bontenbal, Gopuraja Dharmalingam, Thomas Carroll, John B. Gurdon, Amanda G. Fisher, Matthias Merkenschlagerof DpnII (R0543M, New England Biolabs ) overnight at 37°C for Nanog and Lefty1 and 750 U of PvuII (R0151M, New England Biolabs) for 2 h followed by an additional 750 U overnight at 37°C for Klf4. BAC templates

(6) A long ncRNA links copy number variation to a polycomb/trithorax epigenetic switch in FSHD muscular dystrophyCellMay 11, 2012Cabianca DS, Casa V, Bodega B, Xynos A, Ginelli E, Tanaka Y, Gabellini D.formaldehyde for 10′ at room temperature. Digestion was performed with 600–800 U of PvuII (50U/ml, NEB, R0151M) at 37°C overnight with constant agitation.An appropriate amount of DNA that would amplify within the

(7) Fabrication of DNA Polymer Brush Arrays by Destructive Micropatterning and Rolling-Circle AmplificationMacromolecular BioscienceMay 12, 2011Kristopher D. Barbee, Matt Chandrangsu, Xiaohua Huangwith the wash buffer and then the reaction buffer. A solution containing 2 U · μL−1 of PvuII (Cat. # R0151M, New England Biolabs) in a digestion buffer (0.050 m potassium acetate, 0.020 m Tris acetate, 0.010

(8) Fusion-Defective Gibbon Ape Leukemia Virus Vectors Can Be Rescued by Homologous but Not Heterologous Soluble Envelope ProteinsJournal of VirologyMay 1, 2002Karen B. Farrell, Yuan-Tsang Ting, Maribeth V. Eiden

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PvuII

PvuII from New England Biolabs

Description

Sequence

New England Biolabs

Citations (8)

Reviews

PvuII from New England Biolabs

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