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Puromycin
Puromycin Dihydrochloride
Puromycin Dihydrochloride from Fisher BioReagents
Be the first to write a review!
Citations:
(9)
Supplier Page
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Fisher BioReagents for
Puromycin Dihydrochloride
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Description
Inhibits protein synthesis. Selection agent for cells transformed with pac gene. Used for stable transfection of DNA into mammalian cells.
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Puromycin Dihydrochloride
Company
Fisher BioReagents
Price
Supplier Page
View Company Product Page
Catalog Number
BP2956-100
Format
Dry, White or off-white powder
Quantity
100mg
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Fisher BioReagents
Global Chemicals Thermo Fisher Scientific 300 Industry Dr
Pittsburgh
,
Pennsylvania
15275
United States
Phone
: 800.766.7000
800.926.1166
Company Profile
Website:
http://www.fishersci.com/
Citations (9)
(1)
Mpi-driven N-glycosylation orchestrates mucin O-glycosylation and intestinal homeostasis
Nature Communications
May 18, 2026
Avishek Roy, Steve Meregini, Hye-Jeong Cho, Zhenglan Chen, Aariz Zaki, Tandav Argula, Bruce Beutler, Jeffrey A. SoRelle
selection, cells were cultured in complete media containing puromycin (10 mg/mL,
Fisher Bioreagents
BP2956100
) or blasticidin (10 mg/mL, Fisher Scientific, MT30100RB). HT-29 MTx, LS174T, 136-7 vector only, 1049-7
(2)
Molecular glue degrader function of SPOP inhibitors enhances STING-dependent immunotherapy efficacy in melanoma models
Journal of Clinical Investigation
December 15, 2025
Zhichuan Zhu, Xin Zhou, Max Xu, Jianfeng Chen, Kevin C. Robertson, Gatphan N. Atassi, Mark G. Woodcock, Allie C. Mills, Laura E. Herring, Gianpietro Dotti,
et al.
previously reported (83, 84), using 200 μg/mL hygromycin (
H3274
,
Sigma-Aldrich
) or 2 μg/mL puromycin (
BP2956100
,
Fisher BioReagents
). Compounds used include 2′3′-cGAMP (tlrl-nacga23-02, InvivoGen), diABZI (
28054
,
(3)
Molecular glue degrader function of SPOP inhibitors enhances STING-dependent immunotherapy efficacy in melanoma models
J Clin Invest
October 28, 2025
Zhichuan Zhu, Xin Zhou, Max Xu, Jianfeng Chen, Kevin C. Robertson, Gatphan Atassi, Mark G. Woodcock, Allie C. Mills, Laura E. Herring, Gianpietro Dotti,
et al.
previously reported (83, 84), using 200 μg/mL hygromycin (
H3274
,
Sigma-Aldrich
) or 2 μg/mL puromycin (
BP2956100
,
Fisher BioReagents
). Compounds used include 2′3′-cGAMP (tlrl-nacga23-02, InvivoGen), diABZI (
28054
,
(4)
YAP1::TFE3 mediates endothelial‐to‐mesenchymal plasticity in epithelioid hemangioendothelioma
Molecular Oncology
August 17, 2025
Ant Murphy, Samuel Hartzler, Paula A. Vargas Carranza, Shyaman Jayasundara, Madison E. Yates, Nimod D. Janson, Bozhi Liu, Annaleigh Benton, Majid Kazemian, Jason A. Hanna
47]. Forty‐eight hours post transduction, cells were selected and maintained in 2 μg·mL−1 puromycin (
BP2956100
,
Fisher Bioreagents
, Waltham, MA, USA) or 500 μg·mL−1 G418 (
BP6735
,
Fisher Bioreagents
). YTS94A and
(5)
GGNBP2 regulates MDA5 sensing triggered by self double-stranded RNA following loss of ADAR1 editing
Science Immunology
November 22, 2024
Jacki E. Heraud-Farlow, Scott R. Taylor, Alistair M. Chalk, Adriana Escudero, Shi-Bin Hu, Ankita Goradia, Tao Sun, Qin Li, Iva Nikolic, Jin Billy Li,
et al.
control (shLuci) or Adar1-targeting shRNAs and selected with puromycin (2 μg/ml;
Fisher Bioreagents
,
BP2956-100
). Once selected, 20,000 cells were seeded onto 12-well plates coated with gelatin 0.1% (w/v). On the
(6)
Foxp1 suppresses cortical angiogenesis and attenuates HIF-1alpha signaling to promote neural progenitor cell maintenance
EMBO reports
May 1, 2024
Buth JE, Dyevich CE, Rubin A, Wang C, Gao L, Marks T, Harrison MR, Kong JH, Ross ME, Novitch BG,
et al
.
initiated 24 h after nucleofection in 2i media containing 1.5 μg/ml puromycin (
Fisher BioReagents
#
BP2956100
) for ~72 h.
Clones
were picked individually, expanded in feeder-free 2i media, and genomic DNA was collected
(7)
Fascin-1 expression is associated with neuroendocrine prostate cancer and directly suppressed by androgen receptor
British Journal of Cancer
October 24, 2023
Anthony Turpin, Carine Delliaux, Pauline Parent, Hortense Chevalier, Carmen Escudero-Iriarte, Franck Bonardi, Nathalie Vanpouille, Anne Flourens, Jessica Querol, Aurélien Carnot,
et al.
(Sigma-Aldrich, TCR000012342, and TRCN0000123039) and selected with puromycin 1 μg/mL (
Fisher Bioreagents
,
BP2956-100
). For lentiviral production, HEK293T cells were
transfected
by adding a mixture of 150 mM NaCl, DNA
(8)
Multifaceted role of RNA editing in promoting loss-of-function of PODXL in cancer
iScience
August 19, 2022
Fu T, Chan TW, Bahn JH, Kim TH, Rowat AC, Xiao X.
25300120
"
DPBS, no calcium, no magnesium
",
Gibco
,Cat#
14190144
Puromycin Dihydrochloride
,
Fisher BioReagents
,Cat#
BP2956100
Lipofectamine™ 3000 Transfection Reagent
,
Thermo Fisher Scientific
,Cat#
L3000015
Polybrene
,
Santa Cruz Biotechnology
(9)
ADAR1-Dependent RNA Editing Promotes MET and iPSC Reprogramming by Alleviating ER Stress
Cell stem cell
August 6, 2020
Guallar D, Fuentes-Iglesias A, Souto Y, Ameneiro C, Freire-Agulleiro O, Pardavila JA, Escudero A, Garcia-Outeiral V, Moreira T, Saenz C,
et al
.
lentivirus
containing the indicated
shRNA
and selected with 2 μg/ml puromycin (
Fisher Bioreagents
,
BP2956-100
) or 250 μg/ml hygromycin (Corning, 30–240-R). Once selection was completed, transduced MEFs were seeded
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Puromycin Dihydrochloride from Fisher BioReagents
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