Description
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen [1]. There is evidence showing that fibroblasts in various organs are intrinsically different [2]. In the lymphoid compartment, fibroblasts construct a unique conduit system with intriguing size exclusion which plays a fundamental role in regulating immune response and homeostasis of tissue fluids [3]. RLF from ScienCell Research Laboratories are isolated from CD® IGS rat lymph node. RLF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RLF are characterized by their spindle-shaped morphology. RLF are negative for mycoplasma, bacteria, yeast, and fungi. RLF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories. Recommended Medium It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RLF in vitro