5x ELISA COATING BUFFER from Bio-Rad (Formerly AbD Serotec)

Product Specs
Item5x ELISA COATING BUFFER
CompanyBio-Rad
Price $105.00Supplier Page View Company Product Page
Catalog NumberBUF030A
Quantity100 ml
ApplicationsELISA
Format5 X Concentrate
TypeAccessory Reagent

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Preparing Adjuvanted Nanoliposomes for Applications Toward Recombinant Influenza Vaccine DevelopmentBIO-PROTOCOLApril 5, 2026Zachary R. Sia, Wei-Chiao Huang, Matthew Willadsen, Hilliard L. Kutscher, Jonathan F. Lovell, Bruce A. Davidsongrade (Thermo Fisher, catalog number: A57775) 6. 5× ELISA coating buffer (Bio-Rad, catalog number: BUF030C) 7. SuperBlockTM blocking buffer (Thermo Fisher, catalog number: 37515) 8. Bovine serum albumin (BSA),

(2) Preparing Adjuvanted Nanoliposomes for Applications Toward Recombinant Influenza Vaccine DevelopmentBio ProtocApril 5, 2026Zachary R. Sia, Wei-Chiao Huang, Matthew Willadsen, Hilliard L. Kutscher, Jonathan F. Lovell, Bruce A. Davidsongrade (Thermo Fisher, catalog number: A57775) 6. 5× ELISA coating buffer (Bio-Rad, catalog number: BUF030C) 7. SuperBlockTM blocking buffer (Thermo Fisher, catalog number: 37515) 8. Bovine serum albumin (BSA),

(3) Replication in human cells supersedes serum antigenic match for the in vivo efficacy of an A/H1N1pdm09 LAIV virusnpj VaccinesNovember 17, 2025Steffan Llewellyn, Katarzyna E. Schewe, Sameer Ayaz, David Burbidge, Shaun Cooper, Jonathan Crowe, Sarah Dodd, Jemma Shapanis, Katie Tudor, Kathryn Ryan, et al.Louis, MO, USA; Cat. No. F3385) in coating buffer (Bio-Rad Laboratories LTD, Hercules, CA, USA; Cat. No. BUF030B) to 25 μg/ml, before dispensing 100 μl into each well of a 96-well Maxisorp plate (Life Technologies,

(4) In vitro detection of canine anti-human antibodies following intratumoral injection of the hu14.18-IL2 immunocytokine in spontaneous canine melanomaPLoS OneAugust 19, 2025Andrew T. Kosharek, Cindy L. Zuleger, William S. Glass, Jens Eickhoff, Paul M. Sondel, David M. Vail, Mark R. Albertini009-000-003, RRID:AB_2337043) (10 μg/mL, 50 μL/well in ELISA Coating Buffer (Bio-Rad Laboratories, Inc. Cat# BUF030C)), which served as the “capture” agent, and incubated overnight at 4°C. The coating solution was removed,

(5) Addressing SARS-CoV-2 evolution: neutralization of emerging variants of concern by the AVX/COVID-12 ‘Patria’ vaccine based on HexaPro-S ancestral Wuhan spike or its updated BA.2.75.2 versionFront ImmunolMay 19, 2025Gregorio Carballo-Uicab, Gabriela Mellado-Sánchez, Edith González-González, Juana Salinas-Trujano, Ivette Mendoza-Salazar, Karina López-Olvera, Keyla M. Gómez-Castellano, Ma. Isabel Salazar, Jesús M. Torres-Flores, Héctor Elías Chagoya-Cortés, et al.SPN-C522r), both at a concentration of 2 µg/mL in coating buffer (BioRad, Berkeley, CA, USA; Cat. No. BUF030C). Plates were washed with phosphate-buffered saline containing 0.1% Tween 20 (PBS-T) (PBS 10X, Gibco,

(6) Discovery and in vitro characterization of a human anti-CD36 scFvFront ImmunolFebruary 4, 2025Cecilia Mata-Cruz, Sandra L. Guerrero-Rodríguez, Keyla Gómez-Castellano, Gregorio Carballo-Uicab, Juan Carlos Almagro, S. Mayra Pérez-Tapia, Marco A. Velasco-Velázquez(Thermo Scientific, Cat. 278743) were coated with Protein L (1µg/mL) in coating buffer (BioRad, Cat. BUF030C) overnight at 4°C. The plates were washed with MPBS-Tween 0.1% (MPBST). Dilutions of each IPTG-induced

(7) A Targetable Secreted Neural Protein Drives Pancreatic Cancer Metastatic Colonization and HIF1α Nuclear RetentionCancer DiscoveryDecember 2, 2024Norihiro Yamaguchi, Y. Gloria Wu, Ethan Ravetch, Mai Takahashi, Abdul G. Khan, Akimasa Hayashi, Wenbin Mei, Dennis Hsu, Shigeaki Umeda, Elisa de Stanchina, et al.was coated with the capture NPTX1 antibody (Tri-I TDI, 1B1) at 10ug/ml in a coating buffer (Bio-Rad, #buf030a) overnight at 4°C. Following to an iterative wash with PBS, the plate was blocked with a blocking solution

(8) A targetable secreted neural protein drives pancreatic cancer metastatic colonization and HIF1a nuclear retentionCancer DiscovDecember 2, 2024Norihiro Yamaguchi, Y Gloria Wu, Ethan Ravetch, Mai Takahashi, Abdul G. Khan, Akimasa Hayashi, Wenbin Mei, Dennis Hsu, Shigeaki Umeda, Elisa de Stanchina, et al.was coated with the capture NPTX1 antibody (Tri-I TDI, 1B1) at 10ug/ml in a coating buffer (Bio-Rad, #buf030a) overnight at 4°C. Following to an iterative wash with PBS, the plate was blocked with a blocking solution

(9) A Novel Tetravalent Bispecific Immune Cell Engager Activates Natural Killer Cells to Kill Cancer Cells without Mediating FratricideAntibodies (Basel, Switzerland)September 10, 2024Yang G, Nikkhoi SK, Owji H, Li G, Massumi M, Cervelli J, Vandavasi VG, Hatefi A.NJ, USA, Cat# CDA-H82E8) or HER2 (AcroBiosystem, HE2-H82E2) protein in coating buffer (Biorad, Cat# BUF030C) was applied onto a 96-well plate and incubated at 4 °C overnight. After one wash with 250 µL DPBS containing

(10) Natural Autoantibodies in Biologic-Treated Rheumatoid Arthritis and Ankylosing Spondylitis Patients: Associations with Vascular PathophysiologyInt J Mol SciMarch 18, 2024Diána Simon, Dorottya Kacsándi, Anita Pusztai, Boglárka Soós, Edit Végh, György Kerekes, Monika Bodoki, Szilvia Szamosi, Gabriella Szűcs, Zoltán Prohászka, et al.ELISA plates were coated at a concentration of 2.25 μg/mL of the antigen in coating buffer (Bio-Rad BUF030, Bio-Rad Laboratories Inc., Hercules, CA, USA) (50 μL/well, 4–6 °C, overnight). After washing and blocking, serum

(11) Following Natural Autoantibodies: Further Immunoserological Evidence Regarding Their Silent Plasticity and Engagement in Immune ActivationInternational Journal of Molecular SciencesOctober 6, 2023David Szinger, Timea Berki, Péter Németh, Szabina Erdo-Bonyar, Diana Simon, Ines Drenjančević, Senka Samardzic, Marija Zelić, Magdalena Sikora, Arlen Požgain, et al.strain (coating concentration: 0.4 µg/mL). The antigens were dissolved in ELISA Coating Buffer (Bio-Rad BUF030) and applied to 96-well plates overnight at 4–6 °C. Blocking was performed for ≥2 h, RT, with our in-house-developed,

(12) Evaluating the Clinical and Immune Responses to Spotted Fever Rickettsioses in the Guinea Pig-Tick-Rickettsia SystemCurr ProtocNovember 1, 2022Stokes JV, Levin ML, Cross CE, Ross AL, Snellgrove AN, Willeford BV, Alugubelly N, Varela-Stokes AS.flasks (Fisher 13‐680‐65 or equivalent)Millipore water (0.2 µm filtered)5× ELISA Coating Buffer (Bio‐Rad BUF030A)ELISA Ultrablock (Bio‐Rad BUF033A)Bacterial Viability Kit (Invitrogen L7012)Tube Revolver/Rotator (Thermo

(13) Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancerThe Journal of biological chemistryApril 1, 2022Cha JH, Chan LC, Wang YN, Chu YY, Wang CH, Lee HH, Xia W, Shyu WC, Liu SP, Yao J, et al.coated with 100 μl of the antigen solution at a concentration of 5 μg/ml in coating buffer (Bio-Rad, #BUF030A) overnight at 4 °C. Purified proteins of FC control (#110-HG-100), EphA1 (#7146-A1), EphA2 (#3035-A2),

(14) In vivo prime editing of a metabolic liver disease in miceScience translational medicineMarch 16, 2022Böck D, Rothgangl T, Villiger L, Schmidheini L, Matsushita M, Mathis N, Ioannidi E, Rimann N, Grisch-Chan HM, Kreutzer S, et al.plates (Thermo Fisher Scientific, cat. no. 439454) diluted in 1×ELISA Coating Buffer (BioRad, cat. no. BUF030A) for 2h at room temperature. After washing in 1 ×ELISA Wash Buffer (Bio-Rad, cat. no. BUF031C), the

(15) Dynamic Features of Herd Immunity: Similarities in Age-Specific Anti-Measles Seroprevalence Data between Two Countries of Different Epidemiological HistoryJournal of Clinical MedicineFebruary 21, 2022Katalin Böröcz, Senka Samardžić, Ines Drenjančević, Ákos Markovics, Tímea Berki, Péter Némethor equivalent) + TMB. Pre-coated high-binding polystyrene plates (antigens were dissolved in Bio-Rad BUF030, overnight, 4–6 °C) were blocked with our self-developed, synthetic blocking buffer. Samples were applied

(16) Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display LibraryAntibodies (Basel, Switzerland)February 10, 2022Mendoza-Salazar I, Gómez-Castellano KM, González-González E, Gamboa-Suasnavart R, Rodríguez-Luna SD, Santiago-Casas G, Cortés-Paniagua MI, Pérez-Tapia SM, Almagro JC.plates were coated with Protein L (1 µg/mL) in coating buffer (BioRad, Berkeley, CA, USA, Cat. No.: BUF030C) overnight at 4 °C. The plates were washed with PBS and blocked with 3% skim milk in PBS-Tween 0.1%

(17) In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levelsNature biotechnologyAugust 1, 2021Rothgangl T, Dennis MK, Lin PJC, Oka R, Witzigmann D, Villiger L, Qi W, Hruzova M, Kissling L, Lenggenhager D, et al.plates (Thermo Fisher Scientific, cat. no. 439454) diluted in 1× ELISA Coating Buffer (BioRad, cat. no. BUF030A) for 2 h at room temperature. After washing in 1× ELISA Wash Buffer (Bio-Rad, cat. no. BUF031C), the

(18) Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS DisordersJournal of Immunology ResearchMay 25, 2021Zsófia Hayden, Szabina Erdő-Bonyár, Beáta Bóné, Noémi Balázs, Kornélia Bodó, Zsolt Illes, Timea Berki, Diána Simonfrom porcine hearts (Sigma-Merck C3260) at a concentration of 2.25 μg/mL in a coating buﬀer (Bio-Rad BUF030) (50 μL/well, 4-6°C, and overnight). After blocking with 0.5 m/m% PVA (~72,000 Mw, 300 μL/well, room

(19) Relationship between natural and infection-induced antibodies in systemic autoimmune diseases (SAD): SLE, SSc and RAClinical and Experimental ImmunologyDecember 16, 2020K. Böröcz, D. Simon, S. Erdő-Bonyár, K. T. Kovács, É. Tuba, L. Czirják, P. Németh, T. Berkimaltose‐binding protein (MBP) on the other half of the plate at 2·5 µg/ml in ELISA coating buffer (Bio‐Rad BUF030) (50 µl/well, 4–6°C, overnight; Biorad, Hercules, CA, USA). Plates were washed with washing buffer (WB)

(20) The Effect of NNK, A Tobacco Smoke Carcinogen, on the miRNA and Mismatch DNA Repair Expression Profiles in Lung and Head and Neck Squamous Cancer CellsCellsApril 21, 2020Doukas SG, Vageli DP, Lazopoulos G, Spandidos DA, Sasaki CT, Tsatsakis A.buffer [protein added to coating buffer and mixed for 15 min; 1X coating buffer diluted from 5X stock (BUF030A; BIORAD) in dH2 O and mixed for 15 min]. The plates were covered and incubated at 4 ◦ C overnight. The

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