Wizard Plus Minipreps DNA Purification System from Promega

Product Specs
ItemWizard Plus Minipreps DNA Purification System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA7510
Time15 minutes or less
VolumeHigh Copy Number Plasmid: 1-3 ml
Quantity250 preps
YieldThe yield will depend on the volume and type of medium, growth conditions, plasmid copy number and host bacterial strain. In general, 1-10µg of high copy number plasmid can be obtained from 1-3ml of culture (e.g. Luria broth) using a standard bacterial host (e.g. JM109 or DH5alpha™).
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification
FormatSilica resin
Sample TypeBacteria

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth ratesSci RepFebruary 9, 2025Ligiane de Oliveira Leme, Mauricio Machaim Franco, Otávio Augusto de Faria, Alexandre Rodrigues Caetano, Juliana Gonçalves de Souza, Luciano de Rezende Carvalheira, Emivaldo de Siqueira Filho, Margot Alves Nunes Dodeout using the GoTaq qPCR Master Mix Kit (Promega®), according to the manufacturer’s instructions in a7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). Each sample was analyzed in triplicates.

(2) Night vision restored in days after decades of congenital blindnessiScienceOctober 21, 2022Samuel G. Jacobson, Artur V. Cideciyan, Allen C. Ho, Alejandro J. Roman, Vivian Wu, Alexandra V. Garafalo, Alexander Sumaroka, Arun K. Krishnan, Malgorzata Swider, Abraham A. Mascio, et al.assays ,, DNA Clean and Concentration-5 kit,Zymo Research,Cat.# D4004 Wizard Miniprep kit,Promega,Cat.# A7510 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Human:

(3) HupZ, a Unique Heme-Binding Protein, Enhances Group A Streptococcus Fitness During Mucosal ColonizationFrontiers in Cellular and Infection MicrobiologyJune 14, 2022Kristin V. Lyles, Lamar S. Thomas, Corbett Ouellette, Laura C. C. Cook, Zehava Eichenbaum15-ml screw-top tubes). Plasmid extractions were performed using the Promega Wizard Miniprep kit (PR-A7510) or the Qiagen Midiprep kit (12123). Genomic DNA was harvested with Invitrogen PureLink (K1820-01).

(4) Purification and cryo-EM structure determination of Arabidopsis thaliana GLR3.4STAR ProtocolsDecember 17, 2021Shanti Pal Gangwar, Marriah N. Green, Maria V. Yelshanskaya, Alexander I. Sobolevsky(0.22 μm),Merck Millipore,Cat#SA1J789H5 Wizard Plus Minipreps DNA Purification System,Promega,Cat#A7510 Phenol:chloroform:isoamyalcohol,Life Technologies,Cat# 15593031 Cellfectin II Reagent,Gibco,Cat#58760 Ethanol

(5) Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on MembranesStructureDecember 3, 2019Sanchaita Das, Andrew W. Malaby, Agata Nawrotek, Wenhua Zhang, Mahel Zeghouf, Sarah Maslen, Mark Skehel, Srinivas Chakravarthy, Thomas C. Irving, Osman Bilsel, et al.Commercial Assays,Critical Commercial Assays ,, Wizard Plus Miniprep DNA Purification Kit,Promega,Cat#A7510 Wizard SV Gel and PCR Cleanup Kit,Promega,Cat#A9281 ,, Deposited Data,Deposited Data,Deposited Data ,, Grp1

(6) Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange FactorsStructureJanuary 1, 2018Andrew W. Malaby, Sanchaita Das, Srinivas Chakravarthy, Thomas C. Irving, Osman Bilsel, David G. LambrightCommercial Assays,Critical Commercial Assays Wizard Plus Miniprep DNA Purification Kit,Promega,Cat#A7510 Wizard SV Gel and PCR Cleanup Kit,Promega,Cat#A9281 ,, Deposited Data,Deposited Data,Deposited Data Grp1

(7) Standardization of DNA Residual Quantification Method of Vero Cell Rabies Vaccine for Human UseThe Open Medicinal Chemistry JournalJune 30, 2017Janeth del Carmen Arias Palacios, Carlos Alberto Barrero Barreto, José Salvador Montaña Lara, Ángela María Londoño NavasExtraction Plasmid DNA was extracted using Wizard® Plus Minipreps DNA Purification System (Promega #A7500) according to the manufacturer’s protocol and evaluated by electrophoresis and spectrophotometry. Copy

(8) Protein Delivery into Plant Cells: Toward In vivo Structural BiologyFrontiers in Plant ScienceApril 19, 2017Cesyen Cedeño, Kris Pauwels, Peter Tompawork using a Wizard Wizard R Plus SV Minipreps DNA Purification System. (Promega Corporation, Cat. No. A7510). Finally, both erd14-rfp and erd10-rfp fusion constructs were confirmed correctly in frame within pK7WGR2.0

(9) Identification of a calcitonin-like diuretic hormone that functions as an intrinsic modulator of the American lobster,Homarus americanus, cardiac neuromuscular systemJournal of Experimental BiologyJanuary 1, 2010A. E. Christie et al.isolated using a Wizard Plus Miniprep DNA Purification System (Promega, Madison, WI, USA; catalog no. A7500). The vector insert was then sequenced on an ABI 3100 16-capillary sequencer (Applied Biosystems Incorporated, Foster

(10) Systematic Deletion and Mitotic Localization of the Nuclear Pore Complex Proteins ofAspergillus nidulansMolecular Biology of the CellDecember 1, 2006Aysha H. Osmani, Jonathan Davies, Hui-Lin Liu, Aaron Nile, Stephen A. Osmania sterile toothpick, and resuspended in 200 ␮l of Cell Lysis Solution from a Promega Mini Prep kit (A7510; Madison, WI). An equal volume of Neutralizing Solution from the kit was added and this material then

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