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Plasmid Purification Kits (Midi)
PureYield Plasmid Midiprep System
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PureYield Plasmid Midiprep System
PureYield Plasmid Midiprep System
PureYield Plasmid Midiprep System from Promega
(1)
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Citations:
(38)
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PureYield Plasmid Midiprep System
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Description
PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture
Product Specs
Company Info
Citations
Reviews
Product Reviews
Product Specs
Item
PureYield Plasmid Midiprep System
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
A2495
Method
silica-membrane column
Quantity
100 preps
Source
Bacterial
Nucleic Acid Type
Plasmid DNA
Applications
purification
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (38)
(1)
Occludin Regulates HIV-1 Infection by Modulation of the Interferon Stimulated OAS Gene Family
Molecular neurobiology
September 1, 2023
Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M.
Cat#
C737303
) and isolated using PureYield Plasmid midi-prep system (
Promega
, Madison, WI, USA, Cat#
A2492
). Viral stocks were created as described [15]. Briefly, a total of 50 μg of proviral plasmid was
transfected
(2)
Protocol to establish an oviduct epithelial cell line derived from Gallus gallus using Percoll for in vitro validation of recombinant proteins
STAR Protoc
August 4, 2023
Chiron Loubser, Natalya V. Nikitina
cabinet,Esco,Cat#2120759
Econo-Pac Chromatography Columns
,
Bio-Rad
,Cat#
7321010
PureYield Plasmid Midiprep System
,
Promega
,Cat#
A2492
10K MWCO
Vivaspin 6
concentrator column,
Sartorius
,Cat#VS.0601 VWR Instructor’s Dissecting Kit,VWR,Cat#76457-530 Cohan-Vannas
(3)
Modularity and diversity of target selectors in Tn7 transposons
Molecular Cell
June 15, 2023
Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I. Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S. Makarova,
et al.
Kit,
Qiagen
,
28106
QIAprep Spin Miniprep Kit,
Qiagen
,
27106
PureYield™ Plasmid Midiprep System
,
Promega
,
A2495
Gibson Assembly® Master Mix
,
New England Biolabs
,
E2611
NEBuilder® HiFi DNA Assembly Master Mix
,
New England Biolabs
(4)
Programmable mammalian translational modulators by CRISPR-associated proteins
Nature Communications
April 19, 2023
Shunsuke Kawasaki, Hiroki Ono, Moe Hirosawa, Takeru Kuwabara, Shunsuke Sumi, Suji Lee, Knut Woltjen, Hirohide Saito
Table 5. All Plasmids were purified using the
Midiprep
kit (
QIAGEN
, #
12143
/
12145
or
Promega
, #
A2492
/
A2495
). Construction of IVT (
in vitro transcription
) template The ORF of SpCas9 (for trigger mRNA), 5’-UTR
(5)
Protocol to measure centrosome cohesion deficits mediated by pathogenic LRRK2 in cultured cells using confocal microscopy
STAR Protocols
March 1, 2023
Elena Fdez, Rachel Fasiczka, Antonio Jesús Lara Ordóñez, Belén Fernández, Yahaira Naaldijk, Sabine Hilfiker
assays
,Critical commercial
assays
,Critical commercial
assays
,,
PureYield Plasmid Midiprep System
,
Promega
,Cat#
A2492
,,
Experimental models
: Cell lines,
Experimental models
: Cell lines,
Experimental models
: Cell lines ,, "A549
(6)
Temporal Notch signaling regulates mucociliary cell fates through Hes-mediated competitive de-repression
bioRxiv
February 18, 2023
Magdalena Maria Brislinger-Engelhardt, Fabian Lorenz, Maximilian Haas, Sarah Bowden, Alexia Tasca, Clemens Kreutz, Peter Walentek
et al., 2013) was injected at 30ng/μl after
purification
using the PureYield
Midiprep
kit (
Promega
, #
A2492
). For antisense
in situ hybridization
probes, notch1, notch2, notch3, dlc, and jag2 fragments were cloned
(7)
Occludin regulates HIV-1 infection by modulation of the interferon stimulated OAS gene family
Research square
January 30, 2023
Torices S, Teglas T, Naranjo O, Fattakhov N, Frydlova K, Cabrera R, Osborne OM, Sun E, Kluttz A, Toborek M.
Cat#
C737303
) and isolated using PureYield Plasmid midi-prep system (
Promega
, Madison, WI, USA, Cat#
A2492
). Viral stocks were created as described [16]. Briefly, a total of 50 μg of proviral plasmid was
transfected
(8)
PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencing
Molecular cell
December 15, 2022
Fong KW, Zhao JC, Lu X, Kim J, Piunti A, Shilatifard A, Yu J.
Kit,
NEB
,Cat#
E7530S
NEBNext® Ultra™ II
DNA Library Prep
Kit,
NEB
,Cat#
E7645S
PureYield Plasmid Midiprep System
,
Promega
,Cat#
A2495
LR clonase II,Life Tech,11791020
pCR
®8/GW/TOPO® TA Cloning Kit,Life Tech,K250020 X-tremeGENE™ HP
(9)
Systematic multi-omics cell line profiling uncovers principles of Ewing sarcoma fusion oncogene-mediated gene regulation
Cell Reports
December 6, 2022
Martin F. Orth, Didier Surdez, Tobias Faehling, Anna C. Ehlers, Aruna Marchetto, Sandrine Grossetête, Richard Volckmann, Danny A. Zwijnenburg, Julia S. Gerke, Sakina Zaidi,
et al.
kit,"
Macherey-Nagel
, Düren, Germany",Cat#740952
PureYield Plasmid Midiprep System
,"
Promega
, Maddison, WI, USA",Cat#
A2492
Deposited data,Deposited data,Deposited data
WGS
data from 18 EwS cell lines; raw data,This paper,SRA:
(10)
Mass spectrometry analysis of affinity-purified cytoplasmic translation initiation complexes from human and fly cells
STAR Protocols
December 1, 2022
Chingakham Ranjit Singh, Naoki Tani, Akira Nakamura, Katsura Asano
assays
,Critical commercial
assays
,Critical commercial
assays
,, PureYieldTM Plasmid
Midiprep
System,
Promega
,
A2492
Sliver stain II kit WAKO,Wako (Fisher Scientific),NC9535926 ,,
Experimental models
: Cell lines,
Experimental models
(11)
A noncanonical RNA-binding domain of the fragile X protein, FMRP, elicits translational repression independent of mRNA G-quadruplexes
Journal of Biological Chemistry
December 1, 2022
MaKenzie R. Scarpitti, Julia E. Warrick, Evelyn L. Yoder, Michael G. Kearse
#
C404006
), purified using the PureYield
Plasmid Miniprep
or
Midiprep
Systems (
Promega
#
A1222
and
A2495
), and validated by
Sanger sequencing
at The Ohio State University Comprehensive
Cancer
Center Genomics
(12)
Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systems
STAR Protocols
September 16, 2022
Zhiying Zhao, Jan-Fang Cheng, Yasuo Yoshikuni
assays
,Critical commercial
assays
,, Gel extraction kit,
Promega
,Cat#
A9282
Plasmid midi kit,
Promega
,Cat#
A2495
Quant-it dsDNA HS assay kit,
Invitrogen
,Cat#
Q32851
DNeasy blood & tissue Kits,
QIAGEN
,Cat#
69506
,,
Experimental models
(13)
LRH ‐1/ NR5A2 interacts with the glucocorticoid receptor to regulate glucocorticoid resistance
EMBO reports
September 5, 2022
Svenja Michalek, Thomas Goj, Anna Pia Plazzo, Blerim Marovca, Beat Bornhauser, Thomas Brunner
High‐Fidelity PCR Kit,New England BioLabs,Cat# M0553 Pure Yield™ Plasmid
Midiprep
System,
Promega
,Cat#
A2495
QIAquick Gel Extraction Kit,
Qiagen
,Cat#
28706X4
QIAquick PCR Purification Kit,
Qiagen
,Cat#
28104
Quick
(14)
Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement
iScience
June 17, 2022
Fdez E, Madero-Pérez J, Lara Ordóñez AJ, Naaldijk Y, Fasiczka R, Aiastui A, Ruiz-Martínez J, López de Munain A, Cowley SA, Wade-Martins R,
et al
.
assays
,, Gibson Assemby Master Mix,
New England Biolabs
,Cat#
E2611S
PureYield Plasmid Midiprep System
,
Promega
,Cat#
A2492
QuickChange II Site-Directed Mutagenesis Kit,
Agilent Technologies
,Cat#
200523
Duolink In Situ PLA probe anti-rabbit PLUS
(15)
A SARS-CoV-2 antibody broadly neutralizes SARS-related coronaviruses and variants by coordinated recognition of a virus-vulnerable site
Immunity
October 12, 2021
Onodera T, Kita S, Adachi Y, Moriyama S, Sato A, Nomura T, Sakakibara S, Inoue T, Tadokoro T, Anraku Y,
et al
.
Minipreps
DNA Purification
Systems,
Promega
,Cat#
A1460
PureYield Plasmid Midiprep System
,
Promega
,Cat#
A2495
R-Phycoerythrin Labeling Kit - NH2
,
DOJINDO
,Cat#
LK23
Allophycocyanin Labeling Kit - NH2
,
DOJINDO
,Cat#
LK21
QIAseq
(16)
Decentralizing Cell-Free RNA Sensing With the Use of Low-Cost Cell Extracts
Frontiers in Bioengineering and Biotechnology
August 23, 2021
Anibal Arce, Fernando Guzman Chavez, Chiara Gandini, Juan Puig, Tamara Matute, Jim Haseloff, Neil Dalchau, Jenny Molloy, Keith Pardee, Fernán Federici
was produced by midi prepping an overnight culture of 200 ml LB with the appropriate
strain
(
Promega
,
A2492
) and cleaned again using
PCR
cleanup (
Promega
,
A6754
). Plasmid DNA inputs were used in a set of concentrations
(17)
Dual modes of CRISPR-associated transposon homing
Cell
April 1, 2021
Makoto Saito, Alim Ladha, Jonathan Strecker, Guilhem Faure, Edwin Neumann, Han Altae-Tran, Rhiannon K. Macrae, Feng Zhang
Kit,
Qiagen
,
28106
QIAprep Spin Miniprep Kit,
Qiagen
,
27106
PureYield™ Plasmid Midiprep System
,
Promega
,
A2495
Gibson Assembly® Master Mix
,
New England Biolabs
,
E2611
NEBuilder® HiFi DNA Assembly Master Mix
,
New England Biolabs
(18)
Disabling VEGF-Response of Purkinje Cells by Downregulation of KDR via miRNA-204-5p
International Journal of Molecular Sciences
February 22, 2021
Julian Gehmeyr, Abdelouahid Maghnouj, Jonas Tjaden, Matthias Vorgerd, Stephan Hahn, Veronika Matschke, Verena Theis, Carsten Theiss
rpm and a midi-preparation was performed the subsequent day using
PureYield Plasmid Midiprep System
(
A2492
;
Promega
). The correct sequence was verified via
standard sequencing
and the resulting plasmid was stored
(19)
Notch signaling induces either apoptosis or cell fate change in multiciliated cells during mucociliary tissue remodeling
Developmental Cell
February 1, 2021
Alexia Tasca, Martin Helmstädter, Magdalena Maria Brislinger, Maximilian Haas, Brian Mitchell, Peter Walentek
Assays
,Critical Commercial
Assays
M-MLV reverse transcriptase
,
Promega
,
M3681
PureYield
Midiprep
kit,
Promega
,
A2495
Ambion mMessage Machine kit,
Thermo Fisher
,
AM1340
Q5 Site-directed mutagenesis kit
,
NEB
,
E0554S
LysoTracker,
Invitrogen
,
L7526
(20)
Mutational resilience of antiviral restriction favors primate TRIM5α in host-virus evolutionary arms races
eLife
September 15, 2020
Jeannette L Tenthorey, Candice Young, Afeez Sodeinde, Michael Emerman, Harmit S Malik
,
NEBuilder HiFi DNA assembly cloning kit
,
NEB
,
E5520S
,Gibson assembly kit Commercial
assay
or kit,PureYield
plasmid miniprep
kit,
Promega
,
A2495
,
Transfection
-quality
plasmid miniprep
(low LPS) Commercial
assay
or kit,NuceloBond Xtra
midiprep
kit,Takara
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Reviews
Write a Review
(1)
5/18/2016
Large High Quality Plasmid DNA Kit in Nominal Cost
Sivaraman Natarajan
Our lab regularly uses promega midi prep kit for large scale plasmid isolation from 100-200ml bacterial culture for transfection. We observed the quality of plamid DNA good enough for transfection. We...
Read Review
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Product Reviews
PureYield™ Plasmid Midiprep System From Promega
Many protein expression procedures require large amounts of pure plasmid DNA. Over the years, a ...
Read More
PureYield™ Plasmid Midiprep System from Promega
In addition to being very simple to use, all reagents can be stored at room temperature. So, no more...
Read More
PureYield Plasmid Midiprep System from Promega
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