TMB Stabilized Substrate for Horseradish Peroxidase from Promega

Product Specs
ItemTMB Stabilized Substrate for Horseradish Peroxidase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberW4121
Quantity200ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Genetic modification, characterization, and co-infection of Franken Sphingomonas and Anaplasma phagocytophilum in tick cellsSTAR ProtocolsSeptember 15, 2023Lorena Mazuecos, Almudena González-García, José de la Fuente1.00416 0.2 cm cuvettes,Bio-Rad,Cat# 1652086 "TMB (3,3′,5,5′-tetramethylbenzidine)",Promega,Cat# W4121 ProLong Antifade reagent containing DAPI,Thermo Fisher Scientific,Cat# P36962 Streptomycin-Penicillin,Thermo Fisher Scientific

(2) Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vectoriScienceMay 19, 2023Mazuecos L, Alberdi P, Hernández-Jarguín A, Contreras M, Villar M, Cabezas-Cruz A, Simo L, González-García A, Díaz-Sánchez S, Neelakanta G, et al.4561043 Bovine serum albumin,Sigma Aldrich,Cat# A9418 "TMB (3,3′, 5,5′- tetramethylbenzidine)",Promega,Cat# W4121 Proteinase K,Dako,Cat# S3020 Proteinase K,Sigma Aldrich,Cat# 70663 ProLong Antifade reagent containing

(3) In silico analysis and in planta production of recombinant ccl21/IL1β protein and characterization of its in vitro anti-tumor and immunogenic activityPLOS ONEAugust 29, 2022Hasan Marashi, Maria Beihaghi, Masoud Chaboksavar, Samad Khaksar, Homan Tehrani, Ardavan AbiriFinally, the protein bands were visualized by staining the membrane with TMB substrate (Promega; Cat.NO W4121). 2.6 Enzyme-linked immunosorbent assay (ELISA) ELISA plates were coated with total soluble proteins

(4) Computational design of a neutralizing antibody with picomolar binding affinity for all concerning SARS-CoV-2 variantsmAbsJanuary 1, 2022Jeong BS, Cha JS, Hwang I, Kim U, Adolf-Bryfogle J, Coventry B, Cho HS, Kim KD, Oh BH.with a goat anti-rabbit IgG (H + L)-HRP (1706515, Bio-Rad) for 1 h. Using TMB Stabilized Substrate (W4121, Promega), viral infection foci were counted with an ImmunoSpot analyzer (2001726, CTL). NC50s were

(5) Additional evidence on the efficacy of different Akirin vaccines assessed on Anopheles arabiensis (Diptera: Culicidae)Parasites & VectorsDecember 1, 2021Blaženka D. Letinić, Marinela Contreras, Yael Dahan-Moss, Ingrid Linnekugel, José de la Fuente, Lizette L. Koekemoerwith TBS and finally developed with tetramethylbenzidine (TMB) stabilised substrate for HRP (Promega, W4121), according to the manufacturer's recommendations. Mouse immunisation The recombinant proteins were

(6) Transgenic soybean expressing a thermostable phytase as substitution for feed additive phytaseScientific ReportsDecember 1, 2019Yu Zhao, Lixia Zhu, Chaoyang Lin, Zhicheng Shen, Chao Xusecondary antibody. TMB stabilized substrate solution for Horseradish Peroxidase (Promega catalog number W4121) was used for detection. Phytase activity assay. Phytase activity was detected in protein extracts from

(7) Characterization of transgenic rice expressing fusion protein Cry1Ab/Vip3A for insect resistanceScientific ReportsDecember 1, 2018Chao Xu, Jiahui Cheng, Haiyan Lin, Chaoyang Lin, Jianhua Gao, Zhicheng Shensecondary antibody. TMB stabilized substrate solution for Horseradish Peroxidase (Promega, catalog number W4121) or 3,3′-diaminobenzidine tetrahydrochloride (DAB, Sigma-Aldrich #D5905,) was used for detection. PageRuler

(8) Nucleosome acidic patch-targeting binuclear ruthenium compounds induce aberrant chromatin condensationNature CommunicationsDecember 1, 2017Gabriela E. Davey et al.Cell Signaling Technology) and the bands were visualized using the 3,3′,5,5′-tetramethylbenzidine substrate for HRP (W4121 Promega, WI, USA). All antibodies were employed at a concentration corresponding to a 1000-fold dilution

(9) Restraint of the G2/M transition by the SR/RRM family mRNA shuttling binding protein SNXAHRB1 in Aspergillus nidulansGeneticsOctober 1, 2014James SW, Banta T, Barra J, Ciraku L, Coile C, Cuda Z, Day R, Dixit C, Eastlack S, Giang A, et al.Protein complexes were detected by a 1-min exposure in 5 ml of TMB stabilized substrate for HRP (Promega W4121). For GFP-NIMECYCLINB detection, 2.5 mg of extracts was separated by SDS-PAGE (10% Tris-glycine gels,

(10) Spring-loaded model revisited: paramyxovirus fusion requires engagement of a receptor binding protein beyond initial triggering of the fusion proteinJournal of virologyDecember 1, 2011Porotto M, Devito I, Palmer SG, Jurgens EM, Yee JL, Yokoyama CC, Pessi A, Moscona A.washes in 0.1% PBS-Tween 20 and a final wash in Mqh20) and detection with tetramethyl benzidine (TMB) (W4121; Promega). Images of the membranes were taken with a Kodak2000MM image station. ␤-Gal complementation-based

(11) Improved extraction of prolamins for gluten detection in processed foodsAgricultural and Food ScienceDecember 4, 2008P. KANERVA, T. SONTAG-STROHM, O. BRINCKto the buffers. After incubation, the membranes were washed and stained with tetramethylbenzidine (W4121, Promega Corp., USA). The third membrane was incubated with the polyclonal anti-gliadin antibody (1/1000

(12) Requirements for CEACAMs and cholesterol during murine coronavirus cell entryJournal of virologyMarch 1, 2004Thorp EB, Gallagher TM.and tetramethylbenzidine-stabilized substrate for horseradish peroxidase from Promega (catalog no. W4121). To measure the cholesterol content in DRM fractions, cells were labeled with [1,2-3H(N)]cholesterol

(13) Melanocortin and MCH precursor-derived NEI effects on striatum–midbrain co-culturesPeptidesApril 27, 1998V Kistler—Heer, M Schlumpf, W Lichtensteiger

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