Taq 2X Master Mix from New England Biolabs

Product Specs
ItemTaq 2X Master Mix
CompanyNew England Biolabs
Price $158.00 Inquire
Catalog NumberM0270L
Quantity500 reactions
Concentration2X
ApplicationsDNA Analysis
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Modified Polycyclic Compounds Rescue Mis-splicing in Myotonic Dystrophy Type 1 Disease ModelsACS Chemical BiologyMarch 20, 2026Jesus A. Frias, Sawyer M. Hicks, Hormoz Mazdiyasni, Subodh K. Mishra, Kahini Sarkar, Clara Yeboah, Noah M. LeFever, Marina M. Scotti, Hana Zeghal, Naomi Brandt, et al.resulting cDNA was used for polymerase chain reaction (PCR) using NEB Taq 2x Master Mix (NEB, cat. no. M0270L). PCR primer sequences and annealing temperatures (T m) are reported in Table . The manufacturer’s

(2) Aptamer-based approaches for sensitive detection and epitope mapping of SARS-CoV-2 spike proteinMolecular Therapy Nucleic AcidsMarch 12, 2026Suttinee Poolsup, Elnaz Yaghoobi, Aliaksandra Radchanka, Nandanee Mulloo, Spencer Uguccioni, John Paul Pezacki, Abdullah Khraibah, Aasha Jawad, Gurcharan K. Uppal, Yuxuan Gu, et al.from ACRObiosystems. Salt-tolerant T4-DNA ligase (Cat. no. M0467S) and Taq 2× Master Mix (Cat. no. M0270L) were purchased from New England Biolabs. Phosphate buffer saline (PBS) without Ca2+-Mg2+ (Cat. no.

(3) Protocol for conditional gene expression in Magnaporthe oryzae via fungal nitrate reductase promoter replacementSTAR ProtocolsMarch 1, 2026Mostafa Rahnama, Justin King,Zymo Research,D4019 Q5 High-Fidelity 2x Master Mix,New England Biolabs,M0492S Taq 2x Master Mix,New England Biolabs,M0270L AMPure XP Beads for DNA Cleanup,Beckman Coulter,A63880 PerfeCTa SYBR Green FastMix,Quantobio,95072–250 SuperScript™ III First-Strand Synthesis System

(4) Unlocking CRISPR-Cas9 editing for widely diverse Dictyostelid speciesMolecular Systems BiologyJanuary 12, 2026Mireia Garriga-Canut, Nikki Cannon, Matt Benton, Andrea Zanon, Samuel T Horsfield, Jacob Scheurich, Kim Remans, John Lees, Alexandre Paix, Jordi van GestelK,NEB,P8107S Q5 Hot Start High-Fidelity 2X Master Mix,NEB,M0494L Taq 2X Master Mix - 500 reactions,NEB,M0270L Software,Software,Software Snapgene,Dotmatics,https://www.snapgene.com Cas designer,RGEN tools,http://www.rgenome.net/cas-designer/ Other,Other,Other BD FACSAria Fusion Flow Cytometer

(5) RNA-stabilized coat proteins for sensitive and simultaneous imaging of distinct single mRNAs in live cellsNature MethodsJanuary 1, 2026Christopher J. Kuffner, Alexander M. Marzilli, John T. Ngo5’-GGGGAGATTCAGTGTGGTGG-3’; Kwon et. al. 2021).66 Amplifications were performed with Taq 2X Master Mix (NEB, M0270L) using 20 μL reaction volumes containing 1 μL of cDNA or no-RT control template solutions (or 1 ng of

(6) Calcium and Cadmium Activate ESRRB to Mediate Cell Stemness and PluripotencyInternational Journal of Molecular SciencesDecember 25, 2025Xu Shi, Gai Yan, Nicole C. Zhao, Qiaochu Wang, Dajun Lu, Destiny Lawler, Reem M. Gahtani, Celia Byrne, Bassem R. Haddad, Robert L. Copeland, et al."New England Biolabs, Ipswich, MA, USA",R3189 Taq 2X Master Mix,"New England Biolabs, Ipswich, MA, USA",M0270L T4 DNA Ligase,"New England Biolabs, Ipswich, MA, USA",M0202 Maxima H MA Synthesis Master Mix with

(7) High-resolution genetic maps and QTL mapping applications reveal polycystin gene involvement in oyster shell formationiScienceDecember 19, 2025Haitao Ma, Yanping Qin, Dongmei Yu, Bingke Jiao, Qingliang Liao, Yang Zhang, Yinjie Zhang, Jingyue Huang, Jun Li, Ziniu Yu, et al.China",Cat#C112-01 2×RealStar Green Power Mixture,"Genstar, China",Cat#A314-10 Taq master mix,NEB,Cat#M0270L ,, Deposited data,Deposited data,Deposited data ,, GBS reads,This paper,NCBI-SRA (PRJNA967088) ,, Experimental models

(8) IMAGE: INTEGRATE-Mediated Agrobacterium Genome EngineeringFrontiers in MicrobiologyNovember 6, 2025Ephraim Aliu, Liang-Chun Chen, Keunsub Lee, Kan Wang)Q5 High-Fidelity 2X Master Mix (NEB, cat. no. M0492S/L)Taq 2 × Master Mix for genotyping only (NEB, cat. no. M0270L)Thermocycler (Analytikjena, cat. no. Biometra TAdvanced) or preferred vendor.Sterile nuclease-free water

(9) DNA bendability regulates transcription factor binding to nucleosomesNature Structural & Molecular BiologyNovember 1, 2025Luca Mariani, Xiao Liu, Kwangwoon Lee, Stephen S. Gisselbrecht, Philip A. Cole, Martha L. Bulykspecific sequence by PCR, a PCR Master Mix was prepared by thawing 2.5 ml of Taq 2x Master Mix (NEB, M0270L) on ice and adding 2.5 ml cold pure water. The template and the primers were added to the PCR Master

(10) Long-read sequencing reveals the RNA isoform repertoire of neuropsychiatric risk genes in human brainGenome BiologySeptember 23, 2025Ricardo De Paoli-Iseppi, Shweta S. Joshi, Josie Gleeson, Yair D. J. Prawer, Yupei You, Ria Agarwal, Anran Li, Anthea Hull, Eloise M. Whitehead, Yoonji Seo, et al.sequence length and/or nucleotide composition. Novel exons were amplified using Taq 2X MasterMix (NEB, M0270L), and cycling conditions can be found in Additional file 1: Table S7. PCR products were visualized via

(11) Bisulfite‐free PCDHGB7 methylation in urine enables early noninvasive detection of urothelial carcinomaBioengineering & Translational MedicineFebruary 26, 2025Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Zhanrui Mao, Yaping Dong, Wei Li, Wenxuan Li, Yang Han, Lihe Dai, et al.PCR amplification as described previously. 21 Amplifications were performed using the Taq 2× Master Mix kit (NEB, M0270L). The PCR program was set as 30 s at 98°C for pre‐denaturation, followed by 45‐cycle amplification (98°C

(12) Clonal Lineage Tracing with Somatic Delivery of Recordable Barcodes Reveals Migration Histories of Metastatic Prostate CancerCancer DiscoveryOctober 4, 2024Ryan N. Serio, Armin Scheben, Billy Lu, Domenic V. Gargiulo, Lucrezia Patruno, Caroline L. Buckholtz, Ryan J. Chaffee, Megan C. Jibilian, Steven G. Persaud, Stephen J. Staklinski, et al.sample, depending on tumor purity, for analysis. Prior to BC10 analysis, we added Taq 2x Master Mix (M0270L, New England Biolabs) and performed PCR on extracted DNA (95°C for 30 seconds, 54°C for 30 seconds,

(13) Genetic interaction mapping of Aurora protein kinases in mouse oocytesFrontiers in Cell and Developmental BiologySeptember 25, 2024Cecilia S. Blengini, Karen Schindler5′-GTAGGTGGAAATTCTAGCATCATCC-3′). Primers were used at a final concentration of 1 μM, and Taq 2xMaster Mix (NEB, #M0270L) was used following the manufacturer’s protocol. The sizes of products were: Aurka: 243 bp for WT and

(14) Development and characterization of human T-cell receptor (TCR) alpha and beta clones’ library as biological standards and resources for TCR sequencing and engineeringBiology Methods and ProtocolsSeptember 5, 2024Yu-Chun Wei, Mateusz Pospiech, Yiting Meng, Houda AlachkarS1. Amplification was performed in a 25 µl reaction volume consisting of 12.5 µl Taq 2X Master Mix (#M0270L, New England BioLabs Inc., MA, USA), 1.25 µl of 10 µM forward primer, 1.25 µl of 10 µM reverse primer,

(15) Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic controlMetabolic EngineeringSeptember 1, 2024Jennifer N. Hennigan, Romel Menacho-Melgar, Payel Sarkar, Maximillian Golovsky, Michael D. Lynchcolonies were selected for colony PCR with Taq polymerase (Taq 2X Master Mix, NEB, Ipswich, MA, Cat#M0270L), then sent for sequencing for final confirmation. Primers used for colony PCR and sequencing inserts

(16) Spatio-temporal requirements of Aurora kinase A in mouse oocyte meiotic spindle buildingiScienceAugust 16, 2024Cecilia S. Blengini, Michaela Vaskovicova, Jan Schier, David Drutovic, Karen Schindler5′-GTAGGTGGAAATTCTAGCATCATCC-3′). Primers were used at a final concentration of 1 μM, and Taq 2xMaster Mix (NEB, #M0270L) was used following the manufacturer’s protocol. The sizes of products were for Aurka: 243 bp for WT

(17) Cell-type-specific Labeling of Endogenous Proteins Using the Split GFP System in DrosophilabioRxivAugust 2, 2024Melissa Ana Inal, Kota Banzai, Rie Kamiyama, Daichi Kamiyamaneeded for detailed orientation validation.For PCR reaction: Taq 2x Master Mix (New England Biolabs Inc, M0270L). 4. Tissue-specific Reconstitution GFP11 knockin line (generated in the previous step)Gal4 driver

(18) Hypermethylated TAGMe as a universal-cancer-only methylation marker and its application in diagnosis and recurrence monitoring of urothelial carcinomaJournal of Translational MedicineJuly 2, 2024Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Wanxiang Zheng, Zhanrui Mao, Chengchen Qian, Xiangyi Zheng, Lihe Dai, Chengyang Wang, et al.for subsequent PCR amplification. Amplification was performed using the Taq 2 × Master Mix Kit (NEB, M0270L). The PCR program was set to pre-denaturation at 98 °C for 30 s, followed by 45 cycles of amplification

(19) CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryosEMBO reportsOctober 9, 2023Knoblochova L, Duricek T, Vaskovicova M, Zorzompokou C, Rayova D, Ferencova I, Baran V, Schultz RM, Hoffmann ER, Drutovic D.The size of the Zp3‐Cre transgene was 139 bp. The Taq 2× Master Mix was used for genotyping (NEB, # M0270L). Cdc25a mKO genotyping was performed by PCR analysis using the following primers for Cdc25a mKO (Zp3‐Cre):

(20) CRISPR/Cas9-mediated knock-in in ebony gene using a PCR product donor template in DrosophilaGene and Genome EditingJuly 10, 2023Kathy Clara Bui, Daichi Kamiyamaused the genomic DNA prepared from individual flies and was amplified using Taq 2X Master Mix (NEB #M0270L). Thermocycling conditions are as followed: 95 °C for 30 s (denaturation), 95 °C 30 s, 60 °C 30 s, 68

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Taq 2X Master Mix

Taq 2X Master Mix from New England Biolabs

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Taq 2X Master Mix from New England Biolabs

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