Description
KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolution for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides.
The KAPA3G Plant PCR Kits are optimized for fast and efficient amplification of plant DNA from crude samples, DNA-containing carry-over inhibitors from crude extraction methods, and purified DNA.
Key features of the KAPA3G Plant PCR Kit include:
- Fast PCR direct from plant tissues such as leaf discs, seeds and crude plant extracts.
- Streamlined workflows for transgenic screening.
- Improved PCR success rates and reproducibility.
- Efficient amplification of long and difficult targets from all sample types.
Kits include KAPA3G Plant DNA Polymerase (2.5 U/µL), KAPA Plant PCR Buffer with dNTPs (2X, with 1.5 mM MgCl2 and 0.2 mM of each dNTP at 1X), KAPA Plant PCR Enhancer (100X), and MgCl2 (25 mM).