ThermoPolReaction Buffer Pack from New England Biolabs

Product Specs
ItemThermoPolReaction Buffer Pack
CompanyNew England Biolabs
Price $25.00 Inquire
Catalog NumberB9004S
Quantity6 ml
ApplicationsDNA Analysis
TypeBuffer
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernpreviously described.51 Briefly, each 20 µl reaction contained 1× Thermopol Reaction Buffer (NEB, Cat. no. B9004S), 125 µM dNTPs (Thermo Fisher Scientific, Cat. no. 10297018), 2.5 mM MgSO4 (NEB, Cat. no. B1003S), 1X

(2) Oxalate induces proliferation and mitochondrial metabolism in select clear cell renal cell carcinoma cell linesBMC CancerMarch 17, 2026Garrett Hendley, Parveen Kumar, Vivek Verma, Mary Doamekpor, Natalie R. Gassman, Christine M. Wright, Hyeyoung Nam, Chen-Han Wilfred Wu, Tanecia MitchellNEB), EndoVIII (5U, cat#M0299, NEB), AAG (5U, cat#M0313, NEB) resuspended in 1X ThermPol buffer (cat#B9004S, NEB) + 200 µg/ml BSA (cat#B4287-25G, Sigma Aldrich, St. Louis, MO, USA) for 1 h at 37 °C in a hybridization

(3) Cell-type-specific RNA polymerase II activity maps in intact tissues provide a gateway to mammalian gene regulatory mechanisms in vivoDevelopmental CellFebruary 11, 2026Gopal Chovatiya, Sean Y. Huang, Alex B. Wang, Philip Versluis, Chris K. Bai, Michael DeBerardine, Yu-Ching Liao, Judhajeet Ray, Abdullah Ozer, John T. Lis, et al.carried out by first resuspending beads with nascent RNA in RppH reaction mix (10x ThermoPol buffer (NEB, B9004S), RppH (NEB, M0356S), SUPERase-In RNase Inhibitor) and incubating at 37°C for 45 minutes on thermomixer

(4) Identification of intestinal enteroendocrine cell subtypes and their associated hormones in zebrafishPLoS BiolDecember 18, 2025Margaret Morash, Richard G. Kay, Erik J. Soderblom, Grace H. MacLean, Jia Wen, Peyton J. Moore, Colin R. Lickwar, Mujahid Ali Shah, Julia Ganz, Fiona M. Gribble, et al.tissue was incubated in 25 µL of 1× lysis buffer [per 1 mL: 100 µL of 10× PCR buffer (BioLabs NEB, B9004S), 50 µL ∼20 mg/mL of proteinase K (ThermoFisher, 100005393), and 850 µL of nuclease-free water] at 55°C

(5) USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimensPLoS BiolDecember 15, 2025Ya-Wen Hsueh, Po-Min ChiangP2139); Sepharose CL-6B (GE Healthcare, CAS 62610-50-8); HindIII-HF (NEB, R3104S); ThermoPol buffer (NEB, B9004S); carboxylate paramagnetic particles (Cytiva, 65152105050250); Halt protease inhibitor (ThermoFisher);

(6) Click Detect: A Rapid and Sensitive Assay for Shiga Toxin 2 DetectionBiosensors (Basel)December 14, 2025Benjamin M. Thomas, Emma L. Webb, Katherine L. Yan, Alexi M. Fernandez, Zhilei Chenstand, washed 4 times with 250 µL of PTBD, resuspended in 10 µL of 1xNEB ThermoPol buffer (NEB, Cat# B9004S, Ipswich, MA, USA), heated to 95 °C for 5 min, and finally, used for NAATs. For target detection in

(7) Single cell transcriptional perturbome in pluripotent stem cell modelsMolecular Systems BiologyDecember 10, 2025Elisa Balmas, Maria L Ratto, Kirsten E Snijders, Silvia Becca, Carla Liaci, Irene Ricca, Giorgio R Merlo, Raffaele A Calogero, Luca Alessandrì, Sasha Mendjan, et al.Rapid DNA Ligation kit,Invitrogen,#K1423 SPRIselect beads,Beckman Coulter,#B23318 ThermoPol Reaction Buffer (10X),New England Biolabs,#B9004S Deep Vent DNA Polymerase (2 U/µL),New England Biolabs,#M0258S Q5 Hot Start High-Fidelity DNA Polymerase

(8) Effect of 1Bx7null on Soft Wheat Cookie Quality Under Different Nitrogen Inputs and Its CAPS Marker DevelopmentFoodsDecember 2, 2025Pingping Zhang, Guicheng Song, Yuntao Zhang, Jinbao Yao25 µL, consisted of the following: 1 µL genomic DNA (20 ng), 2.5 µL 10×thermalPol® reaction buffer (B9004S) (New England Biolabs (Beijing) Co., Ltd., Beijing, China), 0.5 µL dNTP (10 mM), 0.5 µL forward primer

(9) Paleogenomic evidence on the temporal continuity of indigenous goat exploitation in the Canary IslandsiScienceNovember 21, 2025Clara Díaz-Pérez, Jonathan Santana, Kevin G. Daly, Alejandra C. Ordóñez, Javier G. Serrano, Sara B. Armas-Quintana, Emilio Vacas-Fumero, Aitor Brito-Mayor, Simon-Pierre Gilson, Jacob Morales, et al.,Thermo Scientific,Cat#M0201L Tango Buffer (10X),Thermo Scientific,Cat#BY5 ThermoPol® Reaction Buffer,New England Biolabs,Cat#B9004S Tween® 20,VWR Chemicals,Cat#437082Q "UltraPureTM 0.5M EDTA, pH 8.0",Invitrogen,Cat#15575020 "UltraPureTM

(10) Click-code-seq reveals strand biases of DNA oxidation and depurination in human genomeNature Chemical BiologyOctober 31, 2025Vakil Takhaveev, Nikolai J. L. Püllen, Navnit K. Singh, Lucie Lefevre, Emilie A. Aghajani, Sabrina M. Huber, Stefan Schauer, Hailey L. Gahlon, Anna R. Poetsch, Shana J. SturladdNTP blocking, gDNA was first incubated with 1 µl of ENDOIV (NEB, M0304L) in 1× ThermoPol buffer (NEB, B9004S) in a final volume of 20 µl for 20 min at 37 °C. Then, 2.5 µl of ddNTP mix (Jena Biosciences, NU-1019S),

(11) HybriSeq: probe-based device-free single-cell RNA profilingCommun BiolAugust 19, 2025Daniel Foyt, David Brown, Shuqin Zhou, Brittany Moser, Qin Zhu, Zev J. Gartner, Bo Huangof quenching oligo 1 and 2) and then resuspended in ice-cold 1× ThermoPol reaction buffer (NEB ref B9004S). Cells were passed through a 40μm strainer and counted. Cell concentration was normalized to 23,000

(12) CompasSeq: epitranscriptome-wide percentage assessment of metabolite-capped RNA at the transcript resolutionNat CommunJuly 20, 2025Yuanbo Liu, Dean Li, Xueting Wang, Kongyan Niu, Jun Bai, Lefeng Qu, Nan Liu(NEB, catalog: M0356) to RNAs, resuspended in 50 μL of 1× ThermoPol Reaction Buffer (NEB, catalog: B9004S) and incubated at 37 °C for 1 h in the presence of 20 U of RNaseOUT. Excess yDcpS enzyme (400 U, NEB,

(13) Homeostasis of DNA Hemi‐Methylation in Arabidopsis through Methylation Maintenance, DNA Replication, and Nucleosome Positioning MechanismsAdvanced ScienceJuly 11, 2025Hengye Chen, Chenhuan XuPolymerase (EP0402, Thermo Fisher), 3 µL of 10x A‐tailing mix (100 µL of 10x ThermoPol Reaction Buffer (B9004S, NEB), and 1 µL of 100 mm dATP) were added to the eluted DNA, followed by incubation at 37 °C for 30

(14) New molecular components of high and low affinity iron import systems in DrosophilaNat CommunJuly 1, 2025Sattar Soltani, Minyi Yan, Qingxuan Yu, Areeg Abd Elhafiz, Erika Pfriem, Samuel M. Webb, Thomas Kroll, Jahir Marceliano Bahena Lopez, Fanis Missirlis, Kirst King-JonesGibson Assembly Master Mix (NEB, #E2611). Yeast cell PCR screening was performed using Taq-DNA polymerase (NEB, #B9004S). All cloning and sequencing primers are listed in Supplementary Data 1. Inductively coupled plasma

(15) Widespread impact of nucleosome remodelers on transcription at cis-regulatory elementsCell reportsJune 24, 2025Patty BJ, Dhungana R, Hainer SJ.acids,Corning,25-025-CI L-glutamine,Corning,25-005-CI β-mercaptoethanol,Acros Organics,EW-88124-66 Trypsin,Corning,25-052-CI dNTPs,NEB,N0447L 10X Thermo PCR buffer,NEB,B9004S Taq Polymerase,NEB,M0267X Shortcut RNase III,NEB,M0245L FuGene HD,Promega,E2311 T7 polymerase,This

(16) A Rapid F0 CRISPR Screen in Zebrafish to Identify Regulator Genes of Neuronal Development in the Enteric Nervous SystemNeurogastroenterol MotilApril 6, 2025Ann E. Davidson, Nora R. W. Straquadine, Sara A. Cook, Christina G. Liu, Chuhao Nie, Matthew C. Spaulding, Julia Ganzdeletions. Larvae were placed in lysis buffer containing 1X Thermo pol reaction buffer (New England Biolabs, B9004S) and 1 μg/μl Proteinase K (Invitrogen, 25,530,049) in nuclease‐free water, incubating for 50 min at

(17) Mouse promoters are characterised by low occupancy and high turnover of RNA polymerase IIMolecular Systems BiologyMarch 31, 2025Kasit Chatsirisupachai, Christina J I Moene, Rozemarijn Kleinendorst, Elisa Kreibich, Nacho Molina, Arnaud Krebs"GeneRuler 100 bp DNA Ladder, ready-to-use",Thermo,SM0244 ThermoPol Reaction Buffer Pack - 6.0 ml,New England Biolabs,B9004S TRIzol Reagent,Thermo Fisher,15596026 "T4 RNA Ligase 1 (ssRNA Ligase) - 5,000 units",New England Biolabs,M0204L EGTA,Sigma-Aldrich,E3889 RNA 5’ Pyrophosphohydrolase (RppH

(18) Oligonucleotide subsets selection by single nucleotide resolution barcode identificationNat CommunFebruary 12, 2025Woojin Kim, Mingweon Chon, Yoonhae Koh, Hansol Choi, Eunjin Choi, Hyewon Park, Yushin Jung, Taehoon Ryu, Sunghoon Kwon, Yeongjae Choithe beads were washed three times with 1x ThermoPol® Reaction Buffer (New England Biolabs, cat. no. B9004S). For the coupling step, a mixture containing 1 μL of 1 mM 3′-O-azidomethyl-dNTP, 1 μL each of 2 mM

(19) Structural basis of SIRT7 nucleosome engagement and substrate specificityNat CommunFebruary 4, 2025Carlos Moreno-Yruela, Babatunde E. Ekundayo, Polina N. Foteva, Dongchun Ni, Esther Calvino-Sanles, Henning Stahlberg, Beat FierzBiolabs, cat. # N0447) were diluted on ice in ThermoPol reaction buffer (New England Biolabs, cat. # B9004S), and Taq DNA polymerase (120 units, New England Biolabs, cat. # M0273) was added for a total volume

(20) Root-specific theanine metabolism and regulation at the single-cell level in tea plants (Camellia sinensis)eLifeOctober 14, 2024Shijia Lin, Yiwen Zhang, Shupei Zhang, Yijie Wei, Mengxue Han, Yamei Deng, Jiayi Guo, Biying Zhu, Tianyuan Yang, Enhua Xia, et al.Tag DNA polymerase (NEB, Cat. No. M0267V), 0.4 μl Buffer Tag DNA polymerase (5 U/μl, NEB, Cat. No. B9004S), 1.6 μl dNTP (TAKARA, Cat. No. 4030), 0.32 μl DIG-11-dUTP (25 nmol, Sigma, Cat. No. 11093088910), 1

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