Standard Taq Reaction Buffer Pack from New England Biolabs

Product Specs
ItemStandard Taq Reaction Buffer Pack
CompanyNew England Biolabs
Price $25.00 Inquire
Catalog NumberB9014S
Quantity6 ml
ApplicationsDNA Analysis
TypeBuffer
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Modular gene tagging in C. elegansResearch SquareFebruary 10, 2026Erik Jorgensen, Kevin Hefel, Kevin Kruse, Soren B. Jorgensen, Kam Hoe Jorgensen, Ryan Stolley, Matthew S. Stolley, Matthew JorgensenNew England Biolabs (NEB #P8107S). Each PCR reaction contained 7.24 μL water, 1 μL Taq buffer (NEB #B9014S) 0.2 μL 10mM dNTPs (Thermo Fisher, #R0193), 0.2 μL 10mM forward primer, 0.2 μL 10mM reverse primer,

(2) Modular gene tagging in C. elegansbioRxivJanuary 18, 2026Adam Hefel, Kevin Kruse, Kaden Wall, Soren B. Jorgensen, Kam Hoe Ng, Ryan Stolley, Matthew S. Rich, Erik M. JorgensenNew England Biolabs (NEB #P8107S). Each PCR reaction contained 7.24 μL water, 1 μL Taq buffer (NEB #B9014S) 0.2 μL 10mM dNTPs (Thermo Fisher, #R0193), 0.2 μL 10mM forward primer, 0.2 μL 10mM reverse primer,

(3) NEAT and tidy: a novel RT-PCR method for the independent quantification of the NEAT1_1 isoformBiology Methods and ProtocolsOctober 11, 2025Nadine E Smith, Janni Petersen, Michael Z MichaelReagent,Final concentration,Manufacturer,Cat #,Location 10X Taq DNA polymerase buffer,1X,New England Biolabs,B9014S,"Ipswich, MA, USA" 10 mM dNTPs,0.2 mM,Thermo Fisher Scientific,U1511,"Waltham, MA, USA" 10 µM forward

(4) Pseudomonas aeruginosa Dnr-regulated denitrification in microoxic conditionsMicrobiology SpectrumSeptember 2, 2025Stacie Stuut Balsam, Amy Conaway, Dallas L. Mould, Fabrice Jean-Pierre, Deborah A. Hogan10 mM. The first round reaction mixture contained 2.5 µL 10× Standard Buffer (New England Biolabs, #B9014S), 1.5 µL of 50 mM magnesium chloride (MgCl2; New England Biolabs, #B0510A), 0.75 µL of PMFLGM.GB-3a,

(5) Protocol for sample preparation and single-molecule optical tweezers study of nanodisc-embedded ABC transporter OpuASTAR ProtocJune 20, 2025Lyan van der Sleen, Marco van den Noort, Laura-Marie Silbermann, Bert Poolman, Katarzyna TychBiochemicals GmbH,D97002 Tris,ITW Reagents,A2264 Standard Taq reaction buffer,"New England Biolabs, US",B9014S Taq DNA polymerase,"New England Biolabs, US",M0273 Deoxynucleotide (dNTP) solution mix,"New England Biolabs

(6) Ultra-rapid droplet digital PCR enables intraoperative tumor quantificationMedJune 13, 2025Zachary R. Murphy, Emilia C. Bianchini, Andrew Smith, Lisa I. Körner, Teresa Russell, David Reinecke, Nader Maarouf, Yuxiu Wang, John G. Golfinos, Alexandra M. Miller, et al.proteins","Chemicals, peptides, and recombinant proteins" 10X Standard Taq Reaction Buffer,NEB,Cat# B9014S "Hot Start Taq DNA polymerase, 100 U/μL, high concentration",NEB,Cat# M0495B-HC3 (custom order) HaeIII,NEB,Cat#

(7) Rapid and automated interpretation of CRISPR-Cas13-based lateral flow assay test results using machine learning†Sensors & DiagnosticsFebruary 13, 2025Mengyuan Xue, Diego H. Gonzalez, Emmanuel Osikpa, Xue Gao, Peter B. Lillehojadded to a 10 μL reaction solution containing 1 μL of 10× standard Taq buffer (New England Biolabs, B9014S). Annealing was performed in a thermocycler by heating the oligonucleotides to 95 °C and cooling them

(8) Prevalence, Characterization, and Proteomic Relatedness Among β-Lactam-Resistant Bacteria Throughout the Poultry Production Chain in GreeceFoodsJanuary 13, 2025Anestis Tsitsos, Alexandros Damianos, Konstantinos Kiskinis, Vasilios Tsiouris, Ilias Tirodimos, Nikolaos Soultos, Anna Papa, Vangelis EconomouUSA), 0.625U of OneTaq™ DNA Polymerase (M0273S, NEB), 2.5 μL of 10× OneTaq Standard Reaction Buffer (B9014S, NEB), and 2 μL of DNA sample (Table S5). PCR was carried out in a thermal cycler (LabCycler Gradient,

(9) Field-deployable porcine epidemic diarrhea virus diagnostics utilizing CRISPR-Cas13aVirulenceDecember 31, 2024Yuanyuan Wang, Dalin He, Weihua Li, Yaqin Dong, Linlin Fang, Deju Liu, Yi Tang, Shaobo Xiaoevery sample, an annealing reaction (Table S1) was performed using a standard Taq buffer (cat. no. B9014S; New England Biolabs, UK). Inside the PCR thermocycler, annealing of T7–3 G oligonucleotide and crRNA

(10) Prevalence, Characterization, and Epidemiological Relationships between ESBL and Carbapenemase-Producing Escherichia coli, Klebsiella pneumoniae, and Acinetobacter spp. Isolated from Humans and the Kitchen Environment of Two Greek HospitalsAntibiotics (Basel)October 2, 2024Anestis Tsitsos, Alexandros Damianos, Maria Boutel, Panagiota Gousia, Nikolaos Soultos, Anna Papa, Ilias Tirodimos, Vangelis Economoucomprised 0.625U OneTaq™ DNA Polymerase (M0273S, NEB), 2.5 μL 10× OneTaq Standard Reaction Buffer (B9014S, NEB), 200 μM of dNTPs (N0447S, NEB), 0.2–0.5 μM of primers, and 2 μL of DNA sample (Table S12). K.

(11) PLK-1 regulates MEX-1 polarization in the C. elegans zygotebioRxivAugust 1, 2024Amelia J. Kim, Stephanie I. Miller, Elora C. Greiner, Arminja N. Kettenbach, Erik E. Griffinwas performed by picking individual worms into PCR tubes containing 1X Taq reaction buffer (NEB, Cat#B9014S), Proteinase K (Roche; Cat#03115828001) and nuclease free water, freezing at −80°C for at least 15 min,

(12) The effect of pseudoknot base pairing on cotranscriptional structural switching of the fluoride riboswitchNucleic Acids ResearchMay 8, 2024Laura M Hertz, Elise N White, Konstantin Kuznedelov, Luyi Cheng, Angela M Yu, Rivaan Kakkaramadam, Konstantin Severinov, Alan Chen, Julius B Lucksin vitro transcription were synthesized through a 200 μl PCR reaction containing 10× Taq Buffer (NEB, #B9014S), 250 μM dNTP Mix (NEB, #N0447L), 125 nM primer A (Supplementary Table S1), 125 nM primer B (Supplementary

(13) Magnetically Functionalized Hydrogels for High‐Throughput Genomic ApplicationsAdvanced Materials TechnologiesJanuary 22, 2024Evan Lammertse, Siran Li, Jude Kendall, Catherine Kim, Patrick Morris, Nissim Ranade, Dan Levy, Michael Wigler, Eric Brouzesmagnetic separation video of a 500 μL 20% (v/v) BAG suspension in 1X standard Taq reaction buffer (B9014S, New England Biolabs). Each sample was then split into two PCR microtubes to accommodate their lower

(14) Robust and rapid partitioning in thermoplastic†The AnalystJanuary 1, 2024Phenix-Lan Quan, Maria Alvarez-Amador, Yuhe Jiang, Martin Sauzade, Eric Brouzes(Elmhurst, IL, USA), the ring magnets (R844-N52) from K&J Magnetics (Pipersville, PA, USA). The Taq buffer (#B9014S) was purchased from New England Biolabs (Ipswich, MA, USA) and the Tetronic 1307 was a gift from BASF.

(15) The Effect of Pseudoknot Base Pairing on Cotranscriptional Structural Switching of the Fluoride RiboswitchbioRxivDecember 5, 2023Laura M Hertz, Elise N White, Konstantin Kuznedelov, Luyi Cheng, Angela M Yu, Rivaan Kakkaramadam, Konstantin Severinov, Alan Chen, Julius B Lucksin vitro transcription were synthesized through a 200 μL PCR reaction containing 10x Taq Buffer (NEB, #B9014S), 250 μM dNTP Mix (NEB, #N0447L), 125 nM primer A (Supplementary Table S1), 125 nM primer B (Supplementary

(16) Hidden evolutionary constraints dictate the retention of coronavirus accessory genesbioRxivOctober 20, 2023Stephen A. Goldstein, Teagan M. Feeley, Kristina M. Babler, Zoë A. Hilbert, Diane M. Downhour, Niema Moshiri, Nels C. EldeIncubate at 72°C for minutes in 12 μl total volume including 10x Standard Taq Reaction Buffer (NEB Cat. #B9014S), dATP (NEB Cat. #N0440S), Taq DNA Polymerase (NEB Cat. #M0273L), and nuclease-free water. 2) Insertion

(17) Profiling DNA Ligase Substrate Specificity with a Pacific Biosciences Single‐Molecule Real‐Time Sequencing AssayCurrent ProtocolsMarch 7, 2023Alexander T. Duckworth, Katharina Bilotti, Vladimir Potapov, Gregory J. S. LohmanNew England Biolabs, cat. no. M0379S) Standard Taq Reaction Buffer (10 X, New England Biolabs, cat. no. B9014S) Monarch PCR & DNA Cleanup Kit (New England Biolabs, cat. no. T1030L) Ethanol (200 proof, Sigma-Aldrich,

(18) A missense variant in the nuclear localization signal of DKC1 causes Hoyeraal-Hreidarsson syndromeNPJ genomic medicineOctober 30, 2022Chu CM, Yu HH, Kao TL, Chen YH, Lu HH, Wu ET, Yang YL, Lin CH, Lin SY, Tsai MM, et al.markers (OCT4, SOX2, NANOG) using a standard Taq Reaction Buffer Pack (New England Biolabs, Cat. No. B9014S and M0273S) in a T100TM Thermal Cycler (Bio-Rad) under the following conditions: one cycle at 95 °C

(19) Characterization of histone inheritance patterns in the Drosophila female germlineEMBO reportsJuly 5, 2021Kahney EW, Zion EH, Sohn L, Viets-Layng K, Johnston R, Chen X.and protected from light exposure. Prepare a 10 ml PCR consisting of 1 ml 10× Taq buffer (NEB, Cat#B9014S), 200 µl 10 mM dNTPs (Promega, Cat# U151B), 100 µl 200 µM 5’fluor‐labeled forward primer, 100 µl 200

(20) CD4 Inhibits Helper T Cell Activation at Lower Affinity Threshold for Full-Length T Cell Receptors Than Single Chain Signaling ConstructsFrontiers in ImmunologyJanuary 19, 2021Deborah K. Johnson, Wyatt Magoffin, Sheldon J. Myers, Jordan G. Finnell, John C. Hancock, Taylor S. Orton, Stephen P. Persaud, Kenneth A. Christensen, K. Scott WeberActivation were selected from scTCR transcripts replicated by error-prone PCR (Standard Taq, New England BioLabs, B9014S) (44). Afﬁnity libraries were generated using site directed mutagenesis of 5 amino acids in the CDR3b

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