PciI from New England Biolabs

Product Specs
ItemPciI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0655L
Quantity1000 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

A^CATGT

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopyNucleic Acids ResApril 28, 2026Sara De Bragança, Clara Aicart-Ramos, Ángel Rivera-Calzada, Raquel Arribas-Bosacoma, Mark S Dillingham, Oscar Llorca, Fernando Moreno-Herrerosite of the 2x poly-BbvCI plasmid. The recipient plasmid was digested with PciI (New England Biolabs, #R0655S), dephosphorylated with rSAP, and ligated with the phosphorylated duplex using T4 DNA ligase. Following

(2) Enhanced editing of Bifidobacterium lactis using the endogenous Type I-G CRISPR-Cas systemApplied and Environmental MicrobiologyFebruary 18, 2026Ourania Raftopoulou, Kendall Malmstrom, Meichen Pan, Rodolphe BarrangouRegistry). crRNAs were then assembled in the PciI-digested pBC1-cat (pTRK1400) (NEB, Ipswich, MA, USA; Cat# R0655S) using NEBuilder HiFi Assembly Master Mix (NEB, Ipswich, MA, USA; Cat# E2621S), generating the CRISPR-screening

(3) Structural insights into zinc piracy by Neisseria gonorrhoeae to overcome nutritional immunityStructureFebruary 5, 2026Pooneh Tavakoley Gheinani, Aloke Bera, Julie Stoudenmire-Saylor, Yi Lien, Simone A. Harrison, Alison Criss, Walter J. Chazin, Nicholas Noinaj, Cynthia Nau Cornelissen1706404 XmaI,New England BioLabs,Cat# R0180S XhoI,New England BioLabs,Cat# R0146S PciI,New England BioLabs,Cat# R0655L TPEN,EMD Millipore,Cat# 616394 Isopropyl-β-D-thiogalactopyranoside (IPTG-DIOXANE FREE),BACHEM AMERICAS

(4) Making GFP count: a validated framework for absolute protein quantification in precision fermentationAppl Microbiol BiotechnolFebruary 4, 2026Christina Peternell, Philipp Noll, Annette Brümmer-Rolf, Marius Henkelfrom Eurofins Genomics Germany GmbH Hamburg, Germany. The segment of interest was amplified, digested (R0655S PciI and R0581S BseRI, New England Biolabs GmbH, Frankfurt am Main, Germany), and ligated (T4 DNA Ligase

(5) Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosisNucleic Acids ResearchJanuary 8, 2026Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh, et al.PCR (Supplementary Table S5). Amplified fragment was digested with XbaI (NEB R0145S) and PciI (NEB R0655S) restriction enzymes and ligated to the already digested 18-kb plasmid with the same enzymes. The obtained

(6) Multiscale characterization of Caenorhabditis elegans mutants to probe functional mechanisms of human actin pathological variantsiScienceNovember 21, 2025Théo Hecquet, Nadine Arbogast, Delphine Suhner, Anaïs Goetz, Grégory Amann, Selin Yürekli, Fiona Marangoni, Sophie Quintin, Johannes N. Greve, Nataliya Di Donato, et al.,Thermo Fisher Scientific,ER1501 Restriction enzyme: BsmF1,New England Biolabs,R0572L Restriction enzyme: Pcil,New England Biolabs,R0655L Restriction enzyme: BsaBI,Thermo Fisher Scientific,ER1711 Restriction enzyme: AccI,New England Biolabs,R0161L Restriction

(7) Development of DNA markers for assisted selection of cassava resistant to cassava mosaic disease (CMD)Breeding ScienceApril 4, 2025Hiroki Tokunaga, Pham Thi Nhan, Pham Thi Huong, Nguyen Hai Anh, Le Thi Mai Huong, Truong Minh Hoa, Nguyen Thi Huyen Trang, Nguyen Ba Tung, Cu Thi Le Thuy, Xiaofei Zhang, et al.were cleaned up by ethanol precipitation. Each PCR product was cut by restriction enzyme PciI (NEB, R0655), BbsI (NEB, R3539), BccI (NEB, R0704), EcoRI (NEB, R3101) or NcoI (NEB, R3193) according to the manufacturer’s

(8) Loss-of-function mutations in the dystonia gene THAP1 impair proteasome function by inhibiting PSMB5 expressionNat CommunFebruary 10, 2025Dylan E. Ramage, Drew W. Grant, Richard T. Timmsa fragment encoding mClover3 followed by a P2A peptide and a pUC plasmid digested with PciI (NEB, #R0655) and SbfI (NEB, #R3642). The resulting plasmid was transfected into HEK-239T cells along with a PX459

(9) Tumor cell-intrinsic and tumor microenvironmental conditions co-determine signaling by the glycoimmune checkpoint receptor Siglec-7Cellular and molecular life sciences : CMLSMay 30, 2023van Houtum EJH, Kers-Rebel ED, Looman MW, Hooijberg E, Büll C, Granado D, Cornelissen LAM, Adema GJ.Sanger sequencing. Next, the obtained plasmids were linearized using restriction with PciI (New England Biolabs, R0655S) and purified using phenol/chloroform/isoamyl alcohol. Jurkat/MA cells were electroporated with the

(10) Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter GenesMicrobiology SpectrumDecember 21, 2022Chengjin Ye, Luis Martinez-Sobrido31985-070), PacI (New England Biolabs, catalog no. R0547L), PciI (New England Biolabs, catalog no. R0655L), PCR cleanup system (Promega, catalog no. A9285), plasmid maxiprep kit (Omega, catalog no. D6922-04),

(11) A moonlighting function of a chitin polysaccharide monooxygenase, CWR-1, in Neurospora crassa allorecognitioneLifeAugust 30, 2022Tyler C Detomasi, Adriana M Rico-RamÃrez, Richard I Sayler, A Pedro GonÃ§alves, Michael A Marletta, N Louise GlassRedwood City, CA; Cat# 27104) and linearized with NdeI (NEB, R0111S), SspI-HF (R3132S), or PciI (NEB, R0655S) to transform N. crassa. Strain and culture conditions The standard protocols for N. crassa growth,

(12) Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activityMolecular CellMarch 17, 2022Connor A. Tsuchida et al.Biotech,Cat#A600451-0050 BbsI-HF,NEB,Cat#R3539L AgeI,NEB,Cat#R3552L BamHI,NEB,Cat#R0136L KpnI,NEB,Cat#R3142L PciI,NEB,Cat#R0655L "Dulbecco’s Modified Eagle’s Medium, high glucose",Gibco,Cat#11995073 Opti-MEM I Reduced Serum Medium,Gibco,Cat#31985070 Fetal

(13) The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain qualityThe Plant CellMay 5, 2021Yujiao Gao et al.−80°C. Backcrossed lines were obtained as shown in Supplemental Figure S15. The restriction endonuclease PciI (NEB, R0655L) was used to distinguish between TaNAC019-BI and TaNAC019-BII by the Cleaved Amplified Polymorphic Sequence

(14) CRISPR-READI: Efficient generation of knock-in mice by CRISPR RNP Electroporation and AAV Donor InfectionCell ReportsJune 25, 2019Sean Chen, Sabrina Sun, Dewi Moonen, Clancy Lee, Angus Yiu-Fai Yiu-Fai Lee, David V. Schaffer, Lin Heby digesting the PCR products with EcoRI (New England Biolabs, R0101S) or PciI (New England Biolabs, R0655S), respectively, for 4 hours at 37°. For Sox2-P2A-mStrawberry, Sox2-P2A-CreERT2 and Rosa26-FLEX-mStrawberry

(15) A Transgenic tdTomato Rat for Cell Migration and Tissue Engineering ApplicationsTissue Engineering Part C: MethodsMay 1, 2018Brian C. Syverud, Jonathan P. Gumucio, Brittany L. Rodriguez, Olga M. Wroblewski, Shelby E. Florida, Christopher L. Mendias, Lisa M. Larkinwas isolated from pAAV-UbC-tdTomato28 by digestion with PciI and RsrII restriction enzymes (cat. no. R0655S and R0501S; New England BioLabs, Ipswitch, MA). This isolated fragment contained the human ubiquitin C

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PciI

PciI from New England Biolabs

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PciI from New England Biolabs

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