pBR322 DNA-MspI Digest from New England Biolabs

Product Specs
ItempBR322 DNA-MspI Digest
CompanyNew England Biolabs
Price $82.00 Inquire
Catalog NumberN3032S
Range9 - 622 bp
Concentration1,000 µg/ml
Quantity50 gel lanes
ApplicationsDNA Manipulation
Nucleic Acid TypepBR322 DNA
Features (standardized)Unstained
Size Range9 - 622 bp
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) BID-seq for transcriptome-wide quantitative sequencing of mRNA pseudouridine at base resolutionNature ProtocolsFebruary 1, 2024Li-Sheng Zhang, Chang Ye, Cheng-Wei Ju, Boyang Gao, Xinran Feng, Hui-Lung Sun, Jiangbo Wei, Fan Yang, Qing Dai, Chuan He(6X; New England Biolabs, cat. no. B7021S) pBR322 DNA-MspI Digest (New England Biolabs, cat. no. N3032S) Equipment 50 mL round-bottom flask (Synthware) 250 mL Erlenmeyer flask (Synthware) 100 mL separation

(2) Quantitative base-resolution sequencing technology for mapping pseudouridines in mammalian mRNAMethods in EnzymologyNovember 4, 2023Li-Sheng Zhang, Qing Dai, Chuan He(6X) (New England Biolabs, cat. no. B7021S). pBR322 DNA-MspI Digest (New England Biolabs, cat. no. N3032S). MinElute Gel Extraction Kit (QIAGEN). 2.10 NGS sequencing and data analysis NovaSeq 6000 System

(3) Base-resolution quantitative DAMM-seq for mapping RNA methylations in tRNA and mitochondrial polycistronic RNAMethods in EnzymologyNovember 4, 2023Li-Sheng Zhang, Cheng-Wei Ju, Bochen Jiang, Chuan He(6×) (New England Biolabs, cat. no. B7021S). pBR322 DNA-MspI Digest (New England Biolabs, cat. no. N3032S). MinElute Gel Extraction Kit (QIAGEN). 2.9 NGS sequencing and data analysis NovaSeq 6000 System

(4) Transcription factors modulate RNA polymerase conformational equilibriumNature CommunicationsDecember 1, 2022Chengjin Zhu et al.the size of the RNA species. pBR322 vector (1 mg/ml) was digested with restriction enzyme MspI (NEB, #N3032S). 5′-phosphates were removed by adding 20 U of calf intestinal phosphatase (NEB) and the vector fragments

(5) Experimental and computational workflow for the analysis of tRNA pools from eukaryotic cells by mim-tRNAseqSTAR ProtocolsSeptember 1, 2022Andrew Behrens, Danny D. Nedialkova2009)",https://biopython.org/ ,, Other,Other,Other ,, Low Range ssRNA ladder,New England Biolabs,Cat#N0364S pBR322 DNA-MspI Digest,New England Biolabs,Cat#N3032S Disposable homogenizer pestles for 1.5 mL tubes,VWR,Cat#47747-358

(6) Mammalian NET-seq analysis defines nascent RNA profiles and associated RNA processing genome-wideNature ProtocolsMarch 1, 2016Takayuki Nojima, Tomás Gomes, Maria Carmo-Fonseca, Nicholas J ProudfootI8896)Nuclease-free water, not DEPC-Treated (Life technologies, cat. no. AM9937)pBR322 MspI digest (NEB, cat. no. N3032S)γ-32P ATP (Perkin Elmer, 6,000 Ci/mmol, cat. no. NEG502Z)! CAUTION Consult local and institutional rules

(7) Expanded RNA-binding activities of mammalian Argonaute 2Nucleic acids researchDecember 1, 2009Tan GS, Garchow BG, Liu X, Yeung J, Morris JP 4th, Cuellar TL, McManus MT, Kiriakidou M.detected by autoradiography. Two different radiolabeled size markers were used: (M) pBR322/MspI (NEB N3032S), and (M1) Decade RNA marker (Ambion AM7778). Electrophoretic mobility shift assay A total of 10.8

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