PacI from New England Biolabs

Product Specs
ItemPacI
CompanyNew England Biolabs
Price $312.00 Inquire
Catalog NumberR0547L
Concentration4 u/µL
Quantity1250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TTAAT/TAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(81) Derivation and maintenance of mouse haploid embryonic stem cellsNature ProtocolsJuly 1, 2019Ulrich Elling et al.seller. • NlaIII, MseI, PacI and Sbf1 (restriction enzymes; New England Biolabs, cat. nos. R0125S, R0525S, R0547S and R0642S, respectively). • CutSmart Buffer (New England Biolabs, cat no. B7204S). • TaqI (methyltransferase

(82) Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease ModelsJournal of Visualized ExperimentsApril 16, 2019Lidia Tagliafierro, Ekaterina Ilich, Malik Moncalvo, Jeffrey Gu, Ahila Sriskanda, Carole Grenier, Susan K. Murphy, Ornit Chiba-Falek, Boris KantorR0575S EcoRV New England Biolabs R0195S KpnI New England Biolabs R0142S PacI New England Biolabs R0547S SphI New England Biolabs R0182S Materials Company Catalog Number Comments

(83) Chromatin‐Associated RNA Sequencing (ChAR‐seq)Current Protocols in Molecular BiologyApril 1, 2019David Jukam, Charles Limouse, Owen K. Smith, Viviana I. Risca, Jason C. Bell, Aaron F. Straightno. E7335S) CutSmart Buffer (NEB, cat. no. B7204S) PacI restriction enzyme (10 U/μl; NEB, cat. no. R0547S) NEBNext Ultra II Q5 Master Mix (NEB, cat. no. M0544S; this is required in addition to amount included

(84) Epstein-Barr virus BORF2 inhibits cellular APOBEC3B to preserve viral genome integrityNature microbiologyJanuary 1, 2019Cheng AZ, Yockteng-Melgar J, Jarvis MC, Malik-Soni N, Borozan I, Carpenter MA, McCann JL, Ebrahimi D, Shaban NM, Marcon E, et al.5’-NNN NTT AAT TAA TTA AAC GGG CCC CTT GTC GTC-3’. PCR product was digested into NsiI-HF and PacI (NEB R0547) and ligated into pQCXIP digested with SbfI-HF (NEB R3642) and PacI. BORF2 truncation mutants were generated

(85) Inactivating hepatic follistatin alleviates hyperglycemiaNature medicineJuly 1, 2018Tao R, Wang C, Stöhr O, Qiu W, Hu Y, Miao J, Dong XC, Leng S, Stefater M, Stylopoulos N, et al.pAdEasy vector (Agilent) as described earlier50. The constructs were digested by enzyme PacI (NEB, R0547) and then transfected into human embryonic kidney (HEK) 293A cells for adenoviral production. To make

(86) Inducible Genome Editing with Conditional CRISPR/Cas9 MiceG3 (Bethesda, Md.)May 4, 2018Katigbak A, Robert F, Paquet M, Pelletier J.

(87) In vivo Analysis of Cyclic di-GMP Cyclase and Phosphodiesterase Activity in Escherichia coli Using a Vc2 Riboswitch-based AssayBIO-PROTOCOLMarch 5, 2018Ying Liu, Hyunhee Kim, Ute Römling(New England Biolabs, catalog number: R0189S) 20. PacI restriction enzyme (New England Biolabs, catalog number: R0547S) 21. PCR purification kit (QIAGEN, catalog number: 28106) 22. Rapid DNA ligase kit (Roche Diagnostics,

(88) Design and Direct Assembly of Synthesized Uracil-containing Non-clonal DNA Fragments into Vectors by USERTM CloningBIO-PROTOCOLNovember 20, 2017Morten Egevang Jørgensen, Nikolai Wulff, Majse Nafisi, Deyang Xu, Cuiwei Wang, Sophie Konstanze Lambertz, Zeinu Mussa Belew, Hussam Hassan Nour-Eldinand 2010; Geu-Flores et al., 2007) 3. PacI restriction enzyme (New England Biolabs, catalog number: R0547S) 4. Nt.BbvCI nicking enzyme (New England Biolabs, catalog number: R0632S) 5. Cutsmart buffer (included

(89) MacroBac: New technologies for robust and efficient large-scale production of recombinant multi-protein complexesMethods in EnzymologyJuly 3, 2017Scott D. Gradia, Justin P. Ishida, Miaw-Sheue Tsai, Chris Jeans, John A. Tainer, Jill O. FussQiagen mini-prep kit) AsiSI, PacI, and NotI restriction enzymes (New England Biolabs catalog #R0630, R0547, and R0189) 10× CutSmart buffer (New England Biolabs, included with AsiSI and PacI enzymes) Calf

(90) A rapid and effective method for screening, sequencing and reporter verification of engineered frameshift mutations in zebrafishDisease Models & MechanismsJune 1, 2017Sergey V. Prykhozhij, Shelby L. Steele, Babak Razaghi, Jason N. Bermanusing QIAquick Gel Extraction kit (QIAGEN, 28704), digested with PacI and AscI (New England Biolabs, R0547S and R0558S), purified and inserted into pCS2+MCS-P2A-sfGFP using standard cloning methods. At least

(91) Highly Expandable Human iPS Cell–Derived Neural Progenitor Cells (NPC) and Neurons for Central Nervous System Disease Modeling and High‐Throughput ScreeningCurrent Protocols in Human GeneticsJanuary 12, 2017Chialin Cheng, Daniel M. Fass, Kat Folz‐Donahue, Marcy E. MacDonald, Stephen J. HaggartyStart II DNA polymerase (ThermoFisher Scientific cat# F549L) PacI restriction endonuclease (NEB cat# R0547S) Calf intestinal alkaline phosphatase (NEB cat# M0290S) Quick Ligation kit (NEB cat# M2200S) Stbl3 E. coli

(92) Towards Translational ImmunoPET/MR Imaging of Invasive Pulmonary Aspergillosis: The Humanised Monoclonal Antibody JF5 Detects Aspergillus Lung Infections In VivoTheranosticsJanuary 1, 2017Davies G, Rolle AM, Maurer A, Spycher PR, Schillinger C, Solouk-Saran D, Hasenberg M, Weski J, Fonslet J, Dubois A, et al.linearised in a restriction reaction consisting of 176 μl of endonuclease free water, 30 µl of NE buffer 2 (R0547S, New England Biolabs, UK), 3 µl of 100X BSA (R0547S, New England Biolabs) and 5 µl of Pac1 (R0547S,

(93) Single-cell ChIP-seq reveals cell subpopulations defined by chromatin stateNature biotechnologyNovember 1, 2015Rotem A, Ram O, Shoresh N, Sperling RA, Goren A, Weitz DA, Bernstein BE.preparation To minimize the abundance of barcode adaptors concatemers we add 1uL of PacI restriction enzyme (R0547L, NEB, USA) and 2.5ul of NEB Buffer 1 to each sample of 100 cells in 21.5uL of TE and incubate at 37C

(94) Genome Engineering with CRISPR-Cas9 in the Mosquito Aedes aegyptiCell ReportsApril 7, 2015Kathryn E. Kistler, Leslie B. Vosshall, Benjamin J. Matthewsused as a template for a restriction digest using BamHI (R0136, New England Biolabs [NEB]) or PacI (R0547, NEB). Statistics Summary data were plotted using the python packages matplotlib (boxplots) and seaborn

(95) Rapid, modular and reliable construction of complex mammalian gene circuitsNucleic Acids ResearchSeptember 1, 2013Patrick Guye, Yinqing Li, Liliana Wroblewska, Xavier Duportet, Ron Weissvector was digested in a total volume of 20 µl with 4 U of the restriction enzyme FseI or PacI (R0588, R0547 NEB Biolabs) for 3 h, purified using the QIAGEN Qiaquick PCR purification kit and eluted in 30 µl of

(96) Design and generation of recombinant rabies virus vectorsNature ProtocolsAugust 1, 2013Fumitaka Osakada, Edward M. Callawaycat. no. R0642S), SrfI (Toyobo, cat. no. SRF-101), NheI (NEB, cat. no. R0131S), PacI (NEB, cat. no. R0547S), AscI (NEB, cat. no. R0558S), SacII (NEB, cat. no. R0157S) Phusion high-fidelity DNA polymerase (Finnzymes,

(97) An efficient method of directly cloning chimpanzee adenovirus as a vaccine vectorNature protocolsNovember 1, 2010Zhou D, Zhou X, Bian A, Li H, Chen H, Small JC, Li Y, Giles-Davis W, Xiang Z, Ertl HCJ.cat. no. R0142S) PmeI (New England Biolabs, cat. no. R0560S) PacI (New England Biolabs, cat. no. R0547S) XbaI (New England Biolabs, cat. no. R0145S) SnaBI (New England Biolabs, cat. no. R0130S) NdeI

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PacI

PacI from New England Biolabs

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PacI from New England Biolabs

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