PacI from New England Biolabs

Product Specs
ItemPacI
CompanyNew England Biolabs
Price $76.00 Inquire
Catalog NumberR0547S
Concentration4 u/µL
Quantity250 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

TTAAT/TAA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) The Rpd3 histone deacetylase is a critical regulator of temperature-mediated morphogenesis and virulence in the human fungal pathogen HistoplasmaPLOS BiologyMarch 17, 2026Nebat Ali, Mark Voorhies, Rosa A. Rodriguez, Anita SilHistoplasma. Final plasmids were sequence verified for correct assembly and linearized using PacI (NEB R0547L) along with a non-targeting control plasmid before being electroporated into yeast as described previously

(2) Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancerSci AdvMarch 13, 2026Heather Karner, Tabea C. Mittmann, Vicky W. Chen, Ashir A. Borah, Andreas Langen, Hassan Yousefi, Lisa Fish, Balyn W. Zaro, Albertas Navickas, Hani Goodarzisequences and cloned into the pBdLV-Puro-T2A-mCherry reporter vector (10) through PacI digestion (NEB, R0547S) and HiFi assembly (NEB, E2621S) in an arrayed format. The plasmid library was pooled in an equimolar

(3) Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolutioneLifeFebruary 25, 2026Alastair J Tulloch, Ryan Nicholas Delgado, Rinaldo Catta-Preta, Constance L CepkoKit. Cloning into barcoded library The Stagintbc7 barcoded library was linearized with PacI (NEB, cat. #R0547) and EcoRI-HF (NEB, cat. #R3101). The digested vector was purified via 0.75% agarose gel electrophoresis

(4) NRMLncR, a myocyte-enriched long non-coding RNA, enhances myogenesis in mousebioRxivFebruary 17, 2026Yufen Li, Yumei Zhou, Qing Ying Li, Justine Arrington, Mostafa F. Abdelhai, Yubo Wang, Junxiao Ren, Yung-Yu Cheng, Mingyi Xie, W. Andy Tao, et al.Cat#200157) with pAdEasy-1 plasmid. The positive recombinant plasmid was detected by PacI (NEB, cat#R0547S) digestion. For adenovirus generation, HEK293A cells (60–70% confluent) in 10-cm culture dishes were

(5) Zinc accumulation-induced integrated stress response triggers β-cell identity lossCell ResearchJanuary 28, 2026Qing Ma, Wenjun Xu, Xuan Wang, Haoyu Nie, Yukun Gao, Rui Hu, Zhihao Yang, Xushu Wang, Ta Na, Xiangyi Chen, et al.The pAd/CMV/V5-gene-mCherry adenoviral expression clone was digested with PacI (New England Biolabs, #R0547S) to expose the inverted terminal repeat sequences (ITRs), and the digested clone was purified by phenol/chloroform

(6) Assessing the Toxoplasma Tachyzoite Cell Cycle Phases Using Fluorescent Ubiquitination-Based Cell Cycle IndicatorBIO-PROTOCOLJanuary 20, 2026Mrinalini Batra, Elena S. SuvorovaScientific, catalog number: AAA16046AE) 48. PacI endonuclease (New England Biolabs, catalog number: R0547S) 49. NsiI endonuclease (New England Biolabs, catalog number: R0127S) 50. EcoRV endonuclease (New England Biolabs

(7) Pooled CRISPRi screening reveals fungal-specific vulnerabilities across environments and genetic backgroundsbioRxivJanuary 10, 2026Lauren F Wensing, Philippe C Després, Desiree Francis, Meea Fogal, Anthony Hendriks, Nicholas C Gervais, Clara Fikry, Abdul-Rahman Adamu Bukari, Aleeza C Gerstein, Christina A Cuomo, et al.pooled. Briefly, 30 μL of plasmid DNA (~225 ng/μL) was combined with 1 μl of 10,000 U/mL PacI (NEB, cat. R0547L), 5 μL of 10x rCutsmart and 14 μL of Nuclease-free water and was digested overnight at 37 °C static.

(8) Integrins mediate symbiont-specific uptake in cnidarian larvaeEMBO ReportsDecember 16, 2025Victor A S Jones, Melanie Dörr, Isabelle Siemers, Sebastian Rupp, Sami El Hilali, Sara Brites, Joachim M Surm, Ira Maegele, Sebastian G Gornik, Meghan Ferguson, et al.Digoxygenin-labeled probes were generated after linearization with either AscI (R0558S: NEB) or PacI (R0547S: NEB); dependent on final orientation of the probe, using DIG RNA Labeling Kit (11175025910: Roche).

(9) HyperdCas12a-based multiplexed genetic regulation in Candida albicansNucleic Acids ResDecember 11, 2025Nicholas C Gervais, Ruby K J Rogers, Madeleine R Robin, Rebecca S Shapirowere grown up overnight in YPD at 30°C (∼250 rpm). Plasmids were linearized using PacI from NEB (cat R0547L) and left at 37°C for 12 h, followed by a 20 min heat-inactivation step at 65°C. A transformation master

(10) Growth Factor-Independent mTORC1 Signaling Promotes Primary Cilia Length via Suppression of AutophagyiScienceNovember 25, 2025Jong Shin, Khaled Tighanimine, Yann Cormerais, Dean M. Rosenthal, Pratima Niroula, Samuel C. Lapp, Krystle C. Kalafut, Madi Y. Cisse, Sandra Schrötter, Brendan D. ManningQIAquick Gel Extraction Kit (Qiagen; 28704), and inserted into pMXS-IRES-Blast linearized by PacI (NEB; R0547S) and XhoI (NEB; R0146S) using NEBuilder HiFi DNA Assembly (NEB; E2621S). The plasmid was cloned in NEB

(11) Megabase-scale loss of heterozygosity provoked by CRISPR-Cas9 DNA double-strand breaksMolecular CellNovember 20, 2025Samantha B. Regan, Darpan Medhi, Yuanlin Xu, Travis B. White, Yi-Zhen Jiang, Jung Eun Kim, Shih-Chun Wang, Qichen Deng, Su Jia, Dulguun Baasan, et al.,New England BioLabs,Cat# R3539 Opti-MEM reduced serum media,Gibco,Cat# 11058-021 PacI,New England BioLabs,Cat# R0547 Homemade Amaxa-compatible buffer,Maria Jasin Lab,Zhang et al.95 S. pyogenes Cas9 protein,"QB3 MacroLab,

(12) Targeted loss of heterozygosity in Candida albicans using CRISPR-Cas9 reveals the functional impact of allelic variationGENETICSOctober 8, 2025Philippe C Després, Nicholas C Gervais, Meea Fogal, Ruby K J Rogers, Christina A Cuomo, Rebecca S Shapiroin growth phase the following morning. Plasmids were linearized using the PacI enzyme from NEB (cat.R0547L) and left at 37 °C for 12 h followed by a 20 min heat-inactivation step at 65 °C. A transformation master

(13) Regulation of inflammatory responses by pH-dependent transcriptional condensatesCellOctober 2, 2025Wu Z, Pope SD, Ahmed NS, Leung DL, Hong Y, Hajjar S, Krabak C, Zhong Z, Raghunathan K, Yue Q, et al.Science,Cat# 15710 ProLong Glass Antifade Mountant,Invitrogen,Cat# P36984 PacI,New England Biolabs,Cat# R0547S NheI-HF,New England Biolabs,Cat# R3131S MluI-HF,New England Biolabs,Cat# R3198S BamHI-HF,New England Biolabs

(14) Comprehensive profiling of activity and specificity of RNA-guided transposons reveals opportunities to engineer improved variantsNucleic Acids ResearchSeptember 23, 2025Seong Guk Park, Jung-Un Park, Esteban Dodero-Rojas, John A Bryant, Geetha Sankaranarayanan, Elizabeth H Kelloggusing PaqCI (NEB, R0745L) and T4 DNA ligase. The barcoded TnsC library was digested with PacI (NEB, R0547L) and sent to the Hartwell Center in St. Jude Children’s Research Hospital for Revio PacBio sequencing.

(15) The functionally conserved human lncRNA motif GULF lowers glucose and lipid levels in obese miceJournal of Clinical InvestigationSeptember 16, 2025Zhe Li, Sunmi Seok, Chengfei Jiang, Ping Li, Yonghe Ma, Hang Sun, Haiming CaoThermo Fisher Scientific) according to the protocols. Adenoviral plasmids were then digested with PacI enzyme (catalog R0547L, New England Biolabs), purified (MinElute Reaction Cleanup Kit, catalog 28206, QIAGEN), and transfected

(16) A stress-induced paralog of Lhcb4 controls the photosystem II functional architecture in Arabidopsis thalianaNat CommunJuly 26, 2025Roberto Caferri, Qian Zhou, Luca Dall'Osto, Antonello Amelii, Jianyu Shan, Zhenfeng Liu, Roberto Bassiwas cloned into the plant destination vector pBI121 using SbfI (R3642, New England Biolabs) and PacI (R0547, New England Biolabs) restriction enzymes, followed by ligation with T4 DNA Ligase (EL0011, Thermo Fisher Scientific

(17) Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancerbioRxivJune 7, 2025Heather Karner, Tabea Mittmann, Vicky W. Chen, Ashir A. Borah, Andreas Langen, Hassan Yousefi, Lisa Fish, Balyn W. Zaro, Albertas Navickas, Hani Goodarzisequences and cloned into the pBdLV-Puro-T2A-mCherry reporter vector10 through PacI digestion (NEB, R0547S) and HiFi assembly (NEB, E2621S) in an arrayed format. The plasmid library was pooled in an equimolar

(18) The genetic interaction map of the human solute carrier superfamilyMolecular systems biologyJune 1, 2025Wolf G, Leippe P, Onstein S, Goldmann U, Frommelt F, Teoh ST, Girardi E, Wiedmer T, Superti-Furga G.Kit,Qiagen,#51304 QIAGEN Genomic-tip 100/G,Qiagen,#10243 QIAGEN Genomic-tip 500/G,Qiagen,#10262 PacI enzyme,NEB,#R0547S XbaI enzyme,NEB,#R0145S Phusion HF buffer,NEB,#B0518S Pfu-Sso7d polymerase,Self-purified,https://barricklab.org/twiki/bin/view/Lab/ProtocolsReagentsPfuSso7d RNeasy

(19) Construction of a proximity labeling vector to identify protein-protein interactions in human stem cellsPLoS OneMay 30, 2025Rubens Gomes-Junior, Claudia Maria do Nascimento Moreira, Bruno Dallagiovannarespectively. The digestion followed the manufacturer’s instructions as well (NEB - R3552S and NEB - R0547S). The cloning was performed with T4 DNA ligase (Invitrogen – 15224017) for 16 hours at 4°C. The vector

(20) Neutrophil extracellular traps aggravate uremic cardiomyopathy by inducing myocardial fibroblast pyroptosisSci RepMay 20, 2025Ying Xu, Yan Ren, Wenli Zou, Minmin Wang, Xianyun Ye, Wei ShenThese restriction enzymes included BseRI (New England Biolabs, R0581 V), PacI (New England Biolabs, R0547 V), NdeI (New England Biolabs, R0111 V), and BslI (New England Biolabs, R0555 V), which cleaved DNA

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PacI

PacI from New England Biolabs

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PacI from New England Biolabs

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