Treg Suppression Inspector, human from Miltenyi Biotec

(1) Differential regulation of Treg stability in human naïve and effector Treg subsets by TGFβ-signaling via ARKADIA-SKI axisFrontiers in ImmunologySeptember 9, 2025Fan Yang, Alexis Yanes, Miao Li, Patrick Heizer, Ivan Linatoc, Michael E. Stephens, Yang Song, Tatiana Ort, Kyle J. Bednar, Yasuhiro Ikeda, et al.in Treg media excluding IL2, with the presence of human Treg Suppression Inspector (Miltenyi Biotec, 130092909) at a Teff-to-bead ratio of 1:1. The cell mixtures were cultured for 4 days at 37 °C CO2 incubator.

(2) Role of Regulatory T Cells in Chronic Obstructive Pulmonary DiseasePulmonary MedicineMarch 6, 2025Meghashree Sampath, Geetanjali Bade, Randeep Guleria, Anant Mohan, Sudip Sen, Anjana Talwarisolated from CD4+ CD25+ regulatory T cell isolation kit and Human Treg Suppression Inspector beads (#Cat 130-092-909, Miltenyi Biotec Ltd., United Kingdom) were added to round-bottom 96-well plate at 1:1 bead:lymphocyte

(3) Central control of dynamic gene circuits governs T cell rest and activationNatureDecember 11, 2024Maya M. Arce, Jennifer M. Umhoefer, Nadia Arang, Sivakanthan Kasinathan, Jacob W. Freimer, Zachary Steinhart, Haolin Shen, Minh T. N. Pham, Mineto Ota, Anika Wadhera, et al.resting and stimulated Treg cells and Teff cells individually as controls. Treg Suppression Inspector (130-092-909, Miltenyi Biotec) iMACS particles were prepared and added to the appropriate wells according to the

(4) Controlled human hookworm infection remodels plasmacytoid dendritic cells and regulatory T cells towards profiles seen in natural infections in endemic areasNature CommunicationsJuly 16, 2024Mikhael D. Manurung, Friederike Sonnet, Marie-Astrid Hoogerwerf, Jacqueline J. Janse, Yvonne Kruize, Laura de Bes-Roeleveld, Marion König, Alex Loukas, Benjamin G. Dewals, Taniawati Supali, et al.were stimulated with anti–CD2/CD3/CD28-coated beads at a bead to cell ratio of 1:1 (Miltenyi Biotec; 130-092-909). On day 5 of culture, cells were harvested, washed twice in phosphate-buffered saline (PBS), and stained

(5) Co-transplantation of autologous T(reg) cells in a cell therapy for Parkinson's diseaseNatureJuly 1, 2023Park TY, Jeon J, Lee N, Kim J, Song B, Kim JH, Lee SK, Liu D, Cha Y, Kim M, et al.death profiles. C4-mDAPs with or without C4-TREG were co-cultured with TREG suppression inspector (130-092-909, Miltenyi Biotech) for 7 days under in vitro inflammatory conditions. ELISA After co-culture of PBMC

(6) The optimal use of tildrakizumab in the elderly via improvement of Treg function and its preventive effect of psoriatic arthritisFrontiers in immunologyJanuary 1, 2023Fukasawa T, Yamashita T, Enomoto A, Norimatsu Y, Toyama S, Yoshizaki-Ogawa A, Tateishi S, Kanda H, Miyagawa K, Sato S, et al.isolated CD4+CD25+ or CD4+CD25- T cells was assessed using the protocol provided by the manufacturer (130-092-909, Miltenyi-Biotec). The suppressor capacity of T cells was investigated through co-culture assays. The

(7) Short-Chain Fatty Acids Augment Differentiation and Function of Human Induced Regulatory T CellsInternational journal of molecular sciencesMay 20, 2022Hu M, Alashkar Alhamwe B, Santner-Nanan B, Miethe S, Harb H, Renz H, Potaczek DP, Nanan RK.ratio between 1:1 and 1:16 and stimulated with Treg Suppression Inspector (cell: bead ratio of 1:1, 130-092-909, Miltenyi Biotec) in 96 U-bottom plates. The cells were cultured in medium for 3 days under standard

(8) Extricating human tumour immune alterations from tissue inflammationNatureMay 1, 2022Mair F, Erickson JR, Frutoso M, Konecny AJ, Greene E, Voillet V, Maurice NJ, Rongvaux A, Dixon D, Barber B, et al.Dynabeads (Gibco, 11161D, used at a 1:1 bead-to-cell ratio) or with anti-CD3/CD28/CD2 beads (Miltenyi, 130-092-909, Treg Suppression Inspector, also used at a 1:1 bead-to-cell ratio), either with or without recombinant

(9) L-Arginine Depletion Improves Spinal Cord Injury via Immunomodulation and Nitric Oxide ReductionBiomedicinesJanuary 18, 2022Erens C, Van Broeckhoven J, Hoeks C, Schabbauer G, Cheng PN, Chen L, Hellings N, Broux B, Lemmens S, Hendrix S.10% FBS in a 96-well U-bottom plate. Cells were activated with Treg suppression inspector beads (1:1, 130-092-909, Miltenyi Biotec, Leiden, The Netherlands) and supplemented with the indicated concentrations of rArg-I

(10) Dissecting the role of CSF2RB expression in human regulatory T cellsFrontiers in immunologyJanuary 1, 2022Côrte-Real BF, Arroyo Hornero R, Dyczko A, Hamad I, Kleinewietfeld M.(555725, BD Biosciences). Alternatively, Treg inspector beads (anti-CD2/CD3/CD28 mAb-coated beads) (130-092-909, Miltenyi Biotec) at 1 bead:1 cell ratio were used for cell stimulation. Media was supplemented with

(11) Fast and Efficient Genome Editing of Human FOXP3(+) Regulatory T CellsFrontiers in immunologyJanuary 1, 2021Van Zeebroeck L, Arroyo Hornero R, Côrte-Real BF, Hamad I, Meissner TB, Kleinewietfeld M.96-well U-bottom plates in X-vivo + 5% FBS. Cells were stimulated using Treg Suppression Inspector beads (130-092-909, Miltenyi Biotec) on a 1:1 bead to cell ratio and cultured for 4 days before FACS analysis. After incubation,

(12) PPARγ Contributes to Immunity Induced by Cancer Cell Vaccines That Secrete GM-CSFCancer Immunology ResearchJune 1, 2018Girija Goyal, Karrie Wong, Christopher J. Nirschl, Nicholas Souders, Donna Neuberg, Niroshana Anandasabapathy, Glenn Dranoffselection (Miltenyi) and incubated with CD8+ or CD4+ T cells cultured with activating beads (Miltenyi, 130–092-909), according to the manufacturer’s recommendations. PPARγ modulation of human cultures Rosiglitazone

(13) PPARγ Contributes to Immunity Induced by Cancer Cell Vaccines That Secrete GM-CSFCancer Immunol ResApril 18, 2018Girija Goyal, Karrie Wong, Christopher J. Nirschl, Nicholas Souders, Donna Neuberg, Niroshana Anandasabapathy, Glenn Dranoffselection (Miltenyi) and incubated with CD8+ or CD4+ T cells cultured with activating beads (Miltenyi, 130–092-909), according to the manufacturer’s recommendations. PPARγ modulation of human cultures Rosiglitazone

(14) Production of Proinflammatory Cytokines by Monocytes in Liver-Transplanted Recipients with De Novo Autoimmune Hepatitis Is Enhanced and Induces TH1-like Regulatory T CellsJournal of immunology (Baltimore, Md. : 1950)May 15, 2016Arterbery AS, Osafo-Addo A, Avitzur Y, Ciarleglio M, Deng Y, Lobritto SJ, Martinez M, Hafler DA, Kleinewietfeld M, Ekong UD.suppression assay: anti-CD2, anti-CD3 and anti-CD28 Treg Supression Inspector beads from Miltenyi Biotec (130-092-909), CFSE was obtained from Invitrogen (Carlsbad, CA), anti-PDL1 (BioLegend clone 29E.2A3), anti-PD-1 (BD

Be the First to Write a Review!

Treg Suppression Inspector, human from Miltenyi Biotec

Miltenyi Biotec

Miltenyi Biotec

Citations (14)

Reviews

Treg Suppression Inspector, human from Miltenyi Biotec

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map