BLOT-FastStain™ from G Biosciences

Product Specs
ItemBLOT-FastStain™
CompanyG Biosciences
Price $227.00Supplier Page View Company Product Page
Catalog Number786-34
Quantity25 Blots
ApplicationsStaining of membranes for visualization of proteins onto the membrane after Western transfer and dot-blot applications

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G Biosciences

9800 Page Avenue

St. Louis, Missouri 63132

United States

Phone: +800-628-7730
Fax: 314-991-1504

Company Profile

Website: http://www.gbiosciences.com

(1) Distinct Roles of Surface Nanostructure and Polymer Degradation in Fibroblast Response to Device DesignResearch SquareNovember 19, 2025Kendell M. Pawelec, Erik M. Shapiroeither blocked and reprobed, or stained for total protein using the BLOT-Fast Stain (G Biosciences, cat# 786–34) according to manufacturer’s directions. The total protein signal was imaged under visible light on

(2) Distinct Roles of Surface Nanostructure and Polymer Degradation in Fibroblast Response to Device DesignbioRxivOctober 28, 2025Kendell M Pawelec, Erik M. Shapiroeither blocked and reprobed, or stained for total protein using the BLOT-Fast Stain (G Biosciences, cat# 786–34) according to manufacturer’s directions. The total protein signal was imaged under visible light on

(3) Ketogenesis supports hepatic polyunsaturated fatty acid homeostasis via fatty acid elongationSci AdvJanuary 31, 2025Eric D. Queathem, Zahra Moazzami, David B. Stagg, Alisa B. Nelson, Kyle Fulghum, Abdirahman Hayir, Alisha Seay, Jacob R. Gillingham, D. André d'Avignon, Xianlin Han, et al.μm, Thermo Fisher Scientific, 88518), equal loading was assessed using BlotFastStain (G-Biosciences, 786-34), and then membranes were blocked in 5% non-fat dry milk (NFDM) in tris-buffered saline with Tween 20

(4) In vivo micro-computed tomography evaluation of radiopaque, polymeric device degradation in normal and inflammatory environmentsActa biomaterialiaJune 1, 2024Pawelec KM, Hix JML, Troia A, MacRenaris KW, Kiupel M, Shapiro EM.either blocked and reprobed or stained for total protein using the BLOT-Fast Stain (G Biosciences, cat# 786-34) according to manufacturer’s directions. The total protein signal was imaged under visible light on

(5) Material matters: Degradation products affect regenerating Schwann cellsBiomaterials AdvancesMay 1, 2024Kendell M. Pawelec, Jeremy M.L. Hix, Erik M. Shapiroeither blocked and reprobed, or stained for total protein using the BLOT-Fast Stain (G Biosciences, cat# 786–34) according to manufacturer’s directions. The total protein signal was imaged under visible light on

(6) In vivo Biomedical Imaging of Immune Tolerant, Radiopaque Nanoparticle-Embedded Polymeric Device DegradationbioRxivNovember 4, 2023Kendell M. Pawelec, Jeremy M.L. Hix, Arianna Troia, Matti Kiupel, Erik Shapiroeither blocked and reprobed or stained for total protein using the BLOT-Fast Stain (G Biosciences, cat# 786-34) according to manufacturer’s directions. The total protein signal was imaged under visible light on

(7) Functional attachment of primary neurons and glia on radiopaque implantable biomaterials for nerve repairNanomedicine: Nanotechnology, Biology and MedicineAugust 1, 2023Kendell M. Pawelec, Jeremy M.L. Hix, Erik M. Shapiroeither blocked and reprobed, or stained for total protein using the BLOT-Fast Stain (G Biosciences, cat# 786-34) according to manufacturer's directions. The total protein signal was imaged under visible light on

(8) Mitochondrial citrate metabolism and efflux regulate BeWo differentiationScientific reportsMay 6, 2023Mahr RM, Jena S, Nashif SK, Nelson AB, Rauckhorst AJ, Rome FI, Sheldon RD, Hughey CC, Puchalska P, Gearhart MD, et al.,Jackson ImmunoResearch,115-035-003 Supersignal Chemiluminescent West Pico Plus,Thermo Fisher,34577 BLOT-FastStain,G-Biosciences,786-34 Protease inhibitor cocktail,Sigma,P8340 HDAC inhibitor cocktail,Active Motif,37494 RIPA buffer,Sigma,R0278 Immunofluorescence,Immunofluorescence,Immunofluorescence Rabbit

(9) Radiopaque Implantable Biomaterials for Nerve RepairbioRxivJanuary 10, 2023Kendell M Pawelec, Jeremy ML Hix, Erik M Shapiroeither blocked and reprobed, or stained for total protein using the BLOT-Fast Stain (G Biosciences, cat# 786-34) according to manufacturer’s directions. The total protein signal was imaged under visible light on

(10) Diminished ketone interconversion, hepatic TCA cycle flux, and glucose production in D-β-hydroxybutyrate dehydrogenase hepatocyte-deficient miceMolecular MetabolismNovember 1, 2021David B. Stagg, Jacob R. Gillingham, Alisa B. Nelson, Justin E. Lengfeld, D. André d’Avignon, Patrycja Puchalska, Peter A. Crawford(Southern Biotech 4030–05 and 1015–05 respectively). Protein loading was assessed by BlotFastStain (G-Biosciences, 786–34), or by immunoblot against actin (Sigma A2066) and HRP-conjugated goat anti-rabbit IgG. Blots were developed

(11) TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALSeLifeDecember 10, 2020Kathleen M Cunningham et al.,One Shot TOP10 Chemically Competent E. coli,Thermo Fischer Scientific,Cat# C404006, Commercial assay or kit,Faststain,G-Biosciences,Cat #786–34, Commercial assay or kit,SYBR Select Master Mix,Thermo Fischer Scientific,Cat #4472908, "Chemical

(12) TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALSElifeDecember 10, 2020Kathleen M Cunningham, Kirstin Maulding, Kai Ruan, Mumine Senturk, Jonathan C Grima, Hyun Sung, Zhongyuan Zuo, Helen Song, Junli Gao, Sandeep Dubey, et al.,One Shot TOP10 Chemically Competent E. coli,Thermo Fischer Scientific,Cat# C404006, Commercial assay or kit,Faststain,G-Biosciences,Cat #786–34, Commercial assay or kit,SYBR Select Master Mix,Thermo Fischer Scientific,Cat #4472908, "Chemical

(13) G₄C₂ Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTDNeuronSeptember 23, 2020Coyne AN, Zaepfel BL, Hayes L, Fitchman B, Salzberg Y, Luo EC, Bowen K, Trost H, Aigner S, Rigo F, et al.Scientific,Cat#34580 Immonilon ECL Ultra Western HRP Substrate,Millipore,Cat#WBULS0100 BLOT-FASTSTAIN,G Biosciences,Cat#786-34 RNeasy Mini Kit,Qiagen,Cat#74104 Ambion TRIzol Reagent,Thermo Fisher Scientific,Cat#15-596-018 SuperScript IV First-Strand Synthesis System

(14) A Multidisciplinary Approach Reveals an Age-Dependent Expression of a Novel Bioactive Peptide, Already Involved in Neurodegeneration, in the Postnatal Rat ForebrainBrain sciencesJuly 10, 2018Ferrati G, Brai E, Stuart S, Marino C, Greenfield SA.step, membranes were stained to evaluate the transfer quality with Blot FastStain (G-Biosciences, Cat # 786-34, St Louis, MO, USA) following the manufacturer’s indications and imaged using a CCD camera (G-Box, Syngene,

(15) Modulatory Effects of a Novel Cyclized Peptide in Reducing the Expression of Markers Linked to Alzheimer's DiseaseFrontiers in neuroscienceJanuary 1, 2018Brai E, Simon F, Cogoni A, Greenfield SA.P2938, USA). Next, the membranes were temporarily stained with Blot FastStain (G-Biosciences, cat # 786-34, USA), following the manufacturer's instructions, in order to determine the transfer quality and the

(16) A Novel Ex Vivo Model to Investigate the Underlying Mechanisms in Alzheimer's DiseaseFrontiers in cellular neuroscienceJanuary 1, 2017Brai E, Stuart S, Badin AS, Greenfield SA.Mini-PROTEAN TGX Precast Protein Gels (Bio Rad, cat# 456-1094, USA)BLOT-FastStain (G-Biosciences, cat# 786-34, USA)Blotting grade blocker (Bio Rad, cat# 170-6404, USA)Mouse anti-phosphorylated Tau (Thermo Fisher,

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BLOT-FastStain™ from G Biosciences

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BLOT-FastStain™ from G Biosciences

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