SAM2 Biotin Capture Membrane from Promega

Product Specs
ItemSAM2 Biotin Capture Membrane
CompanyPromega
Price $213.00Supplier Page View Company Product Page
Catalog NumberV2861
Quantity96 samples

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Motif-based substrate mapping of the receptor-like cytoplasmic kinase BIK1 reveals novel components and regulatory nodes of plant immunityNature PlantsFebruary 9, 2026Ryan Toth, Sera Choi, Marie Le Naour--Vernet, Florian Schwanke, Jared L. Johnson, Estee E. Tee, Tomer M. Yaron-Barir, Eleanor Khochaba, Paul Derbyshire, Anka Colo, et al.completion of the reaction, the solutions were spotted onto streptavidin-conjugated membranes (Promega, V2861), where the peptides tightly associated through their C-terminal biotinylation. The membranes were rinsed

(2) An atlas of bacterial serine-threonine kinases reveals functional diversity and key distinctions from eukaryotic kinasesScience SignalingMay 6, 2025Brady O’Boyle, Wayland Yeung, Jason D. Lu, Samiksha Katiyar, Tomer M. Yaron-Barir, Jared L. Johnson, Lewis C. Cantley, Natarajan Kannanincubation. After the reaction, solutions were spotted onto streptavidin-conjugated membranes (Promega, V2861), where the biotinylated C-terminal ends of the peptides were tightly associated. The membranes were

(3) Development of mirror-image monobodies targeting the oncogenic BCR::ABL1 kinaseNature CommunicationsDecember 23, 2024Nina Schmidt, Amit Kumar, Lukas Korf, Adrian Valentin Dinh-Fricke, Frank Abendroth, Akiko Koide, Uwe Linne, Magdalena Rakwalska-Bange, Shohei Koide, Lars-Oliver Essen, et al.terminated with 10 µL of 7.5 M Guanidine-HCl were spotted onto a SAM2 Biotin Capture membrane square (V2861, Promega) and further treated according to the instructions of the manufacturer. 32P radiation of membrane

(4) The intrinsic substrate specificity of the human tyrosine kinomeNatureMay 30, 2024Tomer M. Yaron-Barir, Brian A. Joughin, Emily M. Huntsman, Alexander Kerelsky, Daniel M. Cizin, Benjamin M. Cohen, Amit Regev, Junho Song, Neil Vasan, Ting-Yu Lin, et al.the phosphorylation reactions, peptides were spotted onto Streptavidin-conjugated membranes (Promega, V2861), where they associated through their C-terminal biotinylations. The membranes were rinsed to remove

(5) The molecular basis of Abelson kinase regulation by its αI-helixeLifeApril 8, 2024Johannes Paladini, Annalena Maier, Judith Maria Habazettl, Ines Hertel, Rajesh Sonti, Stephan Grzesiekreaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations. After a short (~1 min) drying phase,

(6) The molecular basis of Abelson kinase regulation by its αI-helixElifeApril 8, 2024Johannes Paladini, Annalena Maier, Judith Maria Habazettl, Ines Hertel, Rajesh Sonti, Stephan Grzesiekreaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations. After a short (~1 min) drying phase,

(7) An atlas of substrate specificities for the human serine/threonine kinomeNatureJanuary 26, 2023Jared L. Johnson, Tomer M. Yaron, Emily M. Huntsman, Alexander Kerelsky, Junho Song, Amit Regev, Ting-Yu Lin, Katarina Liberatore, Daniel M. Cizin, Benjamin M. Cohen, et al.completion of the reaction, the solutions were spotted onto streptavidin-conjugated membranes (Promega, V2861), where the peptides tightly associated through their C-terminal biotinylation. The membranes were rinsed

(8) The TORC1 phosphoproteome in C. elegans reveals roles in transcription and autophagyiScienceMay 20, 2022Sewell AK, Poss ZC, Ebmeier CC, Jacobsen JR, Old WM, Han M.guanidine HCl. Equal volumes of each reaction were pipetted onto a SAM2 biotin capture membrane (Promega, V2861) with 2-3 technical replicates and washed thoroughly (2M NaCl, 1% H3PO4). The dry membrane was exposed

(9) Structural Basis for the Activation and Target Site Specificity of CDC7 KinaseStructureAugust 4, 2020Samual D. Dick, Stefania Federico, Siobhan M. Hughes, Valerie E. Pye, Nicola O'Reilly, Peter CherepanovAssays,Critical Commercial Assays,Critical Commercial Assays ,, SAM2 biotin-capture membrane assay,Promega,Cat# V2861 ,, Deposited Data,Deposited Data,Deposited Data ,, Initial model used for molecular replacement,Hughes

(10) Automethylation-induced conformational switch in Clr4 (Suv39h) maintains epigenetic stabilityNatureAugust 1, 2018Nahid Iglesias, Mark A. Currie, Gloria Jih, Joao A. Paulo, Nertila Siuti, Marian Kalocsay, Steven P. Gygi, Danesh Moazedbuffer. Samples were then spotted on a streptavidin matrix (SAM2® biotin capture membrane; Promega, #V2861), which was washed 3 times with 1M NaCl followed by two times with deionized water. After the membrane

(11) Discovery of a selective, substrate-competitive inhibitor of the lysine methyltransferase SETD8Journal of medicinal chemistryAugust 14, 2014Ma A, Yu W, Li F, Bleich RM, Herold JM, Butler KV, Norris JL, Korboukh V, Tripathy A, Janzen WP, et al.mixed. Then 10 μL of mixture was spotted onto a square of SAM2 biotin capture membrane (catalog no. V2861, Promega), allowed absorption for 5 min at room temperature, washed with 2 M of NaCl solution several

(12) Structure of the Catalytic Domain of EZH2 Reveals Conformational Plasticity in Cofactor and Substrate Binding Sites and Explains Oncogenic MutationsPLoS ONEDecember 19, 2013Hong Wu et al.and mixed. A total of 10 µl of the reaction mixture was spotted onto SAM2® Biotin Capture Membrane (cat# V2861, Promega) and placed at room temperature for 5 minutes. Membranes were washed with 2 M NaCl and deionized

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