Fig 1: Clonogenic cell assay performed in six-well plates, with clones produced by LL 24 ATCC ® CCL-151™ human fibroblasts. Steen was previously irradiated for 3 h while circulating in the mini-circuit and samples were taking hourly. a Cells were cultured for 12 days in a mixture of fresh Steen and media (control, upper images) and in a mixture of UVC-treated Steen and media (UVC, bottom images). No cytotoxic effect of the UVC was seen. b Survival fraction curves of LL 24 ATCC ® CCL-151™ human fibroblasts (n = 3 replicates). The survival curves derived from clonogenic assays experiments and are not significantly different, when comparing untreated controls with cells plated with irradiated Steen solution, after one-way ANOVA statistical analysis
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