T/G HA-VSMC from ATCC

Product Specs
ItemT/G HA-VSMC
CompanyATCC
Price $431.00Supplier Page View Company Product Page
Catalog NumberCRL-1999
TypeCell Biology
SpeciesHomo sapiens
TissueHeart, Aorta:Smooth muscle
Cell LineT/G HA-VSMC
Validated Species ReactivityHuman (Hu)
Tissue (standardized)Muscle
Age11 months
ApplicationsThis cell line is a suitable transfection host

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Salidroside attenuates the osteoblastic differentiation of vascular smooth muscle cells via AMPK/AKT signaling pathwayJournal of Molecular HistologyApril 1, 2026Ying Lu, Yi Fengsmooth muscle cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA; Cat. No. CRL-1999). Cells at passages 4–6 were used for all experiments. VSMCs were cultured in RPMI 1640 medium supplemented

(2) Prolonged In Vivo Performance of Electrospun PCL-Based Vascular Grafts in a Large Animal Model: Influence of Material Design and Systemic Drug SupportACS OmegaMarch 24, 2026Suzan Ozdemir, Janset Oztemur-Sezgin, Havva Tezcan-Unlu, Atıf Yolgosteren, Elif Unlu, Abdullah Dogukan Oz, Meric Kocaturk-Guney, Gulsah Cecener, Nihal Yasar Gul Satar, Hande Sezgin, et al.Cytocompatibility Assessment For in vitro analysis, human aortic smooth muscle cells (SMCs) (T/G HA-VSMC, ATCC CRL-1999) and human umbilical vein endothelial cells (HUVEC, ATCC CRL-1730) were sourced from the American Type

(3) A novel protein B2URF3 from Akkermansia muciniphila increased by intermittent fasting alleviates vascular calcificationJournal of NanobiotechnologyJanuary 7, 2026Shi-Yu Zeng, Jiang-Hua Liu, Ying-Ying Xiang, Zhao-Lin Zeng, Zhi-Bo Zhao, Jie zheng, Yi-Fu Liu, Zhi-Rou Lin, Yi-Yi Wang, Chun-Gu Hong, et al.collected for Alizarin Red S staining (ARS) and calcium content assay. Cell culture Human VSMCs (ATCC, CRL-1999) were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 containing 10% fetal bovine serum (FBS

(4) Ginkgolic Acid Inhibits VSMC Proliferation and Migration and Vascular Restenosis by Regulating Cell Cycle Progression and Cytoskeleton Rearrangement Through TCTN1CellsDecember 3, 2025Yuting Shao, Lingyan Yi, Qingyu Zhu, Yulin Zhou, Tingting Chen, Wenjuan YaoTreatment Human aortic VSMCs (HA-VSMCs) were bought from the American Type Culture Collection (ATCC number: CRL-1999; Manassas, VA, USA) and cultured as we previously described [3]. Cells in passages 3–7 were used in

(5) Ultrasmall Cu2-xSe nanoparticles alleviate vascular calcification through inhibiting oxidative stress and NF-κB/NLRP3-mediated inflammationRedox BiolDecember 3, 2025Ding Liu, Yuanzhi Ye, Zirong Lan, An Chen, Xingchen Zhou, Zhenhao Li, Xin Wen, Jisheng Xiao, Fan Ouyang, Jianyun YanRat VSMCs then migrated out from aortic tissues and were utilized between passages 3–8. Human VSMCs (CRL-1999, ATCC, USA) were cultured under identical conditions. Calcifying medium (CM) containing 3 mM calcium

(6) Microscopically Adaptable Bioink Guide Cell Compartmentalization toward Morphogenesis of a Functional Vasculature‐Like SystemAdvanced Healthcare MaterialsNovember 1, 2025Jun Chen, Yuqiong Wu, Jiarong Huang, Junjin Jie, Chan Huang, Di Zhang, Yuxin Huang, Rui Mao, Ming Zhao, Lin‐Ping Wuand 1% penicillin/streptomycin (P/S, Gibco, USA) at under water‐saturated 5% CO2 at 37 °C. VSMCs (#CRL‐1999, ATCC, USA) were maintained in F12K medium (Gibco, USA) supplemented with 10% FBS and 1% P/S at 37 °C

(7) Circ_HUWE1: a novel regulator of lipid accumulation, inflammation, and gut microbiota in atherosclerosisCell BiosciJuly 19, 2025Zulong Sheng, Yi Fan, Zhenjun Ji, Yanru He, Rui Zhang, Yuyu Yao, Genshan Magroups. Cell culture and transfection Human vascular smooth muscle cells (VSMCs) were obtained from ATCC (#CRL-1999) and cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, C0252, Beyotime) in

(8) Extraction of coronary thrombus-derived exosomes from patients with acute myocardial infarction and its effect on the function of adventitial cellsPLoS OneJanuary 16, 2025Youfu He, Bo Wang, Yu Qian, Debin Liu, Qiang Wuvein endothelial cells (HUVEC, Cat.NO. PCS-100-013™) and vascular smooth muscle cells (SMCs, Cat.NO. CRL-1999) were purchased from ATCC (Manassas, VA, USA). Human cardiac myocytes (HCM; cat. 6200) was purchased

(9) lncRNA H19 facilitates vascular neointima formation by targeting miR-125a-3p/FLT1 axisActa Biochimica et Biophysica SinicaSeptember 2, 2024Rengui Jiang, Xuyu He, Weidong Chen, Huoying Cai, Zhaohai Su, Zheng Xie, Bilong Zhang, Jiangyong Yang, Yueting Wang, Ling Huang, et al.elastic lamina (IEL). Cell culture The human aortic vascular smooth muscle cell line T/G HA-VSMCs (ATCC CRL-1999) and human embryonic kidney 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s modified Eagle’s medium (DMEM

(10) Attenuating Atherosclerosis through Inhibition of the NF-κB/NLRP3/IL-1β Pathway-Mediated Pyroptosis in Vascular Smooth Muscle Cells (VSMCs)Cardiovascular TherapeuticsMarch 25, 2024Shihuan Li, Qingjie Li, Qiaofeng Zhou, Suqin Li, Siqi Wang, Qing Yao, Changhan Ouyang, Chao Liu, Mincai LiMaterials and Methods 2.1. Materials 2.1.1. Experimental Cells Human vascular smooth muscle cell line (CRL-1999) was purchased from ATCC. 2.1.2. Experimental Animals Fourteen male SPF grade ApoE-/-male mice, 6-8

(11) S-propargyl-cysteine promotes the stability of atherosclerotic plaque via maintaining vascular muscle contractile phenotypeFrontiers in Cell and Developmental BiologyJanuary 24, 2024Li Ping, Li Zhi-Ming, Zhang Bi-Shan, Zhu Lei, Yu Bo, Zhu Yi-Chun, Wang Ming-Jiethe primers’ sequences we used. Cell culture Human artery vascular smooth muscle cells (HAVSMCs, CRL-1999, ATCC, United States) were cultured in a humidified incubator at 37°C and 5% CO2 using HAVSMC growth

(12) LINC02015 modulates the cell proliferation and apoptosis of aortic vascular smooth muscle cells by transcriptional regulation and protein interaction networkCell Death DiscovAugust 18, 2023Fangyu Liu, Yulin Wang, Xitong Huang, Dingqian Liu, Wenjun Ding, Hao Lai, Chunsheng Wang, Qiang JiSmooth Muscle Cell Medium (#1101, Sciencell, USA). HAVSMC cell line was purchased from American Type Culture Collection (CRL-1999, ATCC, USA) and cultured with Ham’s F-12K medium (Gibco, NY, USA) containing 10% fetal bovine serum

(13) A novel cuproptosis-related diagnostic gene signature and differential expression validation in atherosclerosisMol BiomedJuly 14, 2023Cui Y, Chen Y, Gan N, Li M, Liao W, Zhou Y, Xiang Q, Gong X, Guo Q, Hu P, et al.(Proteintech, 67,735–1-gl), and CD68 (Abcam, ab201340) were used. Cell culture conditions Human aortic SMCs (CRL-1999) were purchased from BeNa Culture Collection (BNCC, Beijing, China) and authenticated by the supplier.

(14) Long noncoding RNA SNHG1 alleviates high glucose-induced vascular smooth muscle cells calcification/senescence by post-transcriptionally regulating Bhlhe40 and autophagy via Atg10Journal of physiology and biochemistryFebruary 1, 2023Li S, Ni Y, Li C, Xiang Q, Zhao Y, Xu H, Huang W, Wang Y, Wang Y, Zhan J, et al.criteria to find differentially expressed lncRNAs. Cell culture HA-VSMCs were obtained from ATCC (ATCC-CRL-1999). The cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Hyclone, USA) with 10% fetal

(15) BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In VivoAging and diseaseFebruary 1, 2023Lin X, Xiang QY, Li S, Song WL, Wang YJ, Ni YQ, Zhao Y, Li C, Wang Y, Li HH, et al.(CACs). MATERIALS AND METHODS Cell culture Human aorta VSMCs (HA-VSMCs) were purchased from ATCC (ATCC-CRL-1999) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS

(16) PKR deficiency delays vascular aging via inhibiting GSDMD-mediated endothelial cell hyperactivationiScienceJanuary 20, 2023Peng Z, Tan X, Xie L, Li Z, Zhou S, Li Y.lines,Experimental models: Cell lines ,, HUVECs,ATCC,"Cat#: CRL-1730, RRID: CVCL_2959" HASMCs,ATCC,"Cat#: CRL-1999, RRID: CVCL_4009" ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(17) Proteins secreted by brain arteriolar smooth muscle cells are instructive for neural developmentMolecular BrainNovember 30, 2022Xuzhao Li, Lili Zhou, Xiaoxuan Zhang, Yuxiao Jin, Bingrui Zhao, Dongdong Zhang, Chengjie Xi, Jiayu Ruan, Zhu Zhu, Jie-Min Jiamedia HBVSMCs, HUVSMCs (ScienCell Research Laboratories, Cat# 1100 and Cat# 8020), and HAVSMCs (ATCC, cat. No CRL-1999) were cultured with smooth muscle cell medium (SMCM) (ScienCell Research Laboratories, Cat# 1101) supplemented

(18) Improved mechanical, degradation, and biological performances of Zn–Fe alloys as bioresorbable implantsBioactive MaterialsNovember 1, 2022Yingchao Su, Jiayin Fu, Wonsae Lee, Shaokang Du, Yi-Xian Qin, Yufeng Zheng, Yadong Wang, Donghui Zhuendothelial cells (EA.hy926, ATCC CRL-2922, US) and human aortic vascular smooth muscle cells (HA-VSMCs, ATCC CRL-1999, US) were cultured in 75 cm2 flask (BD Bioscience) with Dulbecco's Modified Eagle Medium (DMEM, ATCC,

(19) Exosomal STAT1 derived from high phosphorusâ€‘stimulated vascular endothelial cells induces vascular smooth muscle cell calcification via the Wnt/Î²â€‘catenin signaling pathwayInternational Journal of Molecular MedicineOctober 21, 2022Zheng Qin, Yupei Li, Jiameng Li, Luojia Jiang, Zhuyun Zhang, Kaixi Chang, Qinbo Yang, Shanshan Chen, Ruoxi Liao, Baihai SuCell culture and transfection Human aortic VSMCs were purchased from American Type Culture Collection (ATCC-CRL-1999) and HUVECs were purchased from iCell Bioscience, Inc. VSMCs were cultured in DMEM (Gibco; Thermo Fisher

(20) Amphiphilic and fatigue-resistant organohydrogels for small-diameter vascular graftsScience AdvancesJuly 29, 2022Jinfei Hou, Xu Zhang, Yuqiong Wu, Junjin Jie, Zhenxing Wang, Guo-Qiang Chen, Jiaming Sun, Lin-Ping Wuwas evaluated using HUVECs [#PCS-100-010, American Type Culture Collection (ATCC), USA] and VSMCs (#CRL-1999, ATCC, USA). The HUVECs were cultured in RPMI 1640 medium (#C11875500BT, Gibco, USA) supplemented with

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