AML12 from ATCC

Product Specs
ItemAML12
CompanyATCC
Price $0.00Supplier Page View Company Product Page
Catalog NumberCRL-2254
SpeciesMus musculus
TypeCell Biology
Cell Typehepatocyte
Validated Species ReactivityMouse (Ms)
Age3 months
DiseaseNormal
TissueLiver
ApplicationsThis cell line is a suitable transfection host

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Lactobacillus johnsonii mediates the protective effects of pristimerin against ulcerative colitis and concomitant liver injury through remodeling hepatic lipid metabolism via LXRα-SCD1 axisGut MicrobesDecember 31, 2026Yan Cheng, Zhanxuan E. Wu, Ruoyue Huang, Qingmei Li, Dongmei Yan, Yuqi Chen, WeiFeng Zhu, Fei Liconcurrently given vehicle (1% DMSO sterilized water). Cell culture and treatment AML12 cells (ATCC, cat#CRL-2254) were cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin

(2) Microbial metabolite Enterobactin impairs mitochondrial respiration and alleviates colitisGut microbesDecember 31, 2026Kushwaha V, Yeoh BS, Saha P, Chassaing B, Joe B, Gewirtz AT, Vijay-Kumar M.maintained following the standard protocol available on the ATCC website (www.atcc.org/Products/All/CRL-2254.aspx#culturemethod). Recombinant Lcn2 stimulation Mouse recombinant Lcn2 was purchased from Sino Biological Inc

(3) Long-read target enrichment sequencing for rAAV integration site analysis in engineered clones with targeted viral insertionMol Ther Nucleic AcidsSeptember 8, 2026Erica Coratella, Myriam Lemmens, Mevion Oertli, Yoann Gilbart, Kristian Davidsen, Lara Bontadelli, Rebecca Bohnert, Joyce Rigal, Jennifer Bian, Tara Alpert, et al.of predominant events are priorities. Materials and methods MCF10A cell culture MCF10A cells (#CRL2254, ATCC, Manassas, VA, USA) were maintained in DMEM/F12 (#11330, Thermo Fisher Scientific, Zurich, Switzerland)

(4) Phytochemical Profiling, In Vitro Bioactivity, and Network Pharmacology of Astragalus cruciatus Link Ethanolic Extract: Multitarget Mechanisms Underlying Potential Antidiabetic EffectsMoleculesAugust 13, 2026Leila Bellebcir, Imene Derardja, Redouane Rebai, Luc Jasmin, Abdennacer BoudahCo. (St. Louis, MO, USA). The AML12 cell line was obtained from the American Type Culture Collection (CRL-2254, ATCC, Manassas, VA, USA). 4.2. Plant Collection and Identification Plant material was collected in

(5) Myeloid MMP14 couples extracellular proteolysis to inflammatory and metabolic remodeling during obesityCell ReportsJuly 28, 2026Long J. Shao, Fathima Elizondo, Feng Gao, Elizabeth L. Lieu, Bharati Reddi, Maryam Elizondo, Iqbal Mahmud, Kristin Eckel-Mahan, Philipp E. Scherer, Xin Ge, et al.Lines,Experimental models: Cell Lines,Experimental models: Cell Lines Alpha Mouse Liver: AML12 cells,ATCC,Cat#CRL-2254; RRID:CVCL_0140 Mouse: L929 cells,ATCC,Cat#CCL-1; RRID:CVCL_0462 Mouse: RAW264.7 cells,ATCC,Cat#Tib-71;

(6) A Non-Canonical Role for Hepatocyte MLKL in Promoting Mitochondrial Dysfunction and Senescence in the Aging LiverAging CellJuly 3, 2026Sabira Mohammed, Chao Jiang, Travis Pennington, Shylesh Bhaskaran, Phoebe Ohene-Marfo, Kara F. Kneuper, Constantin Georgescu, Kamille Pitts, Albert Tran, Zongkai Peng, et al.UPLC‐MS to profile liver lipid composition. 2.18 Overexpression of MLKL in AML12 Cells AML12 cells (#CRL2254, ATCC) were transfected with cDNA (pcDNA3.1 + C‐DYK, GenScript) or pcDNA‐MLKL‐Flag (ORF Clone, #OMU41768D,

(7) SUCNR1 coordinates metabolic flux, mitochondrial function, and nutrient-dependent adaptation in hepatocytesSci AdvJune 12, 2026Anna Marsal-Beltran, Laura Salmerón-Pelado, Aleix Ribas-Latre, Maria Repollés-de-Dalmau, M-Mar Rodríguez-Peña, Catalina Núñez-Roa, Joan Badia, Ana Madeira, Eva Novoa, Marc Beltrà, et al.from the normal liver of a 3-month-old mouse, were obtained from the American Type Culture Collection (CRL-2254, ATCC) and were cultured in a humidified 5% CO2 incubator at 37°C with DMEM/F12 medium (31330-038, Gibco)

(8) Hepatocyte Circadian Clocks Control Cholesterol Metabolism and Protect From Metabolic Dysfunction-Associated Steatohepatitis (MASH)Cellular and Molecular Gastroenterology and HepatologyMay 12, 2026Leonardo Vinicius Monteiro de Assis, Lina Jegodzinski, Julica Inderhees, Sylvia J. Wowro, Juliana Marques Affonso, Isabel Heyde, Emelie Luise Fischer, Witigo von Schönfels, Andrea Schenk, Florian Roßner, et al.filtered for a score < 400. In Vitro Experiments AML-12 cells, obtained from ATCC Biobank (catalog no. CRL-2254), were cultured in Gibco Dulbecco’s Modified Eagle Medium supplemented with Nutrient Mixture F12 (ThermoFisher),

(9) Evolution-guided protein design of IscB for persistent epigenome editing in vivoNature biotechnologyMay 1, 2026Kannan S, Altae-Tran H, Zhu S, Xu P, Strebinger D, Oshiro R, Faure G, Moeller L, Pham J, Mears KS, et al.Mammalian cell culture experiments were performed in the HEK293FT line (Thermo Fisher Scientific) and AML12 line (CRL-2254, ATCC). HEK293FT cells were grown in Dulbecco’s modified Eagle medium with high glucose, sodium pyruvate

(10) Antioxidant Therapy Reverses Hepatotoxicity Induced by Microcystin-LR in a Cellular Model of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD)Journal of XenobioticsApril 29, 2026Apurva Lad, Jason Kindle, Prajwal Hegde, Gabriel G. Kleer, Andrew L. Kleinhenz, Johnna A. Birbeck, Judy Westrick, Nicholas J. Peraino, Terry D. Hinds, Neeraja Purandare, et al.(alpha mouse liver 12) cell line was obtained from American Type Culture Collection (ATCC) (Cat. No. CRL-2254, ATCC, Manassas, VA, USA). The cells were grown in DMEM/F12 1:1 media (Cat. No. SH30261.01, HyClone

(11) Hepatic GGPP triggers visceral adipose hypertrophy via binding with adipocyte ACSL1 in metabolic unhealthy obesityCommunications BiologyApril 28, 2026Hong-Yu Nie, Ming-Jie Zou, Meng-Fei Zhao, Yi-Ping Tang, Nan-Nan Wang, Zi-Yue Zhou, Jing-Zi Zhang, Chao-Jun Li, Lei Fangline,ATCC Cat#CRL1772,ATCC Cat#CRL1772 293 T cell line,ATCC Cat#CRL-3216,ATCC Cat#CRL-3216 AML12,ATCC Cat#CRL-2254,ATCC Cat#CRL-2254 Experimental models: organisms/strains,Experimental models: organisms/strains,Experimental models

(12) Adiponectin modulates the diurnal hepatic transcriptome and energy metabolism in male miceEndocr ConnectApril 14, 2026Misa Hirose, Enrique Calvo, Violetta Pilorz, Meike Kaehler, Ingolf Cascorbi, Inga Nagel, Leonardo Vinicius Monteiro de Assis, Henrik Osterk (24). Seahorse experiment AML-12 mouse hepatocytes, sourced from the ATCC Biobank (catalog no. CRL-2254) and transduced with Bmal1:Luc plasmid (25), were cultured in Gibco Dulbecco’s modified Eagle’s medium

(13) Dual control of liver regeneration by Nr1d1 homeostasis and Klf2 checkpointCell Death DiscoveryApril 13, 2026Bingyu Ye, Dejian Xie, Wenlong Shen, Meijuan Yue, Qinpeng Jin, Xinjie Guo, Yan Zhang, Ping Li, Zhihu Zhaoacross three independent experiments. Cell culture and transfection Mouse hepatocyte AML12 cells (CRL-2254, ATCC, Manassas, VA, USA) were cultured in AML12 Cell Complete Medium (CM-0602, Procell, Wuhan, China)

(14) Platelets accelerate endoplasmic reticulum stress and promote hepatic steatosisJHEP ReportsApril 1, 2026Martina Derler, Twisha Dube, Elisabeth Ableitner, Waltraud C. Schrottmaier, Manuel Salzmann, Dagmar Kolb, Anderson Massahiro De Campos, Hanna Engelke, Elisabeth Annerer, Natalia Kunowska, et al.Materials and methods. Cell culture The hepatocyte cell line AML12 (ATCC, Manassas, Virginia, USA, CRL-2254™) was cultured in DMEM-F12 medium (Gibco, Waltham, Massachusetts, USA) containing 10% FBS (Sigma, Darmstadt,

(15) Dual-targeted lipid nanoparticles for TET3 siRNA delivery: nanobiotechnology strategy to remodel tumor immune microenvironment in hepatocellular carcinomaJournal of NanobiotechnologyApril 1, 2026Wei Peng, Jin Zhou, Zhiliang Chen, Chengjia Tang, Jia Kuang, Wenke Li, Zhao YangATCC, USA) and Hepa1c1c-7 (CRL-2026, ATCC, USA), along with the murine normal liver cell line AML12 (CRL-2254, ATCC, USA). All cells were cultured in either Dulbecco’s Modified Eagle Medium (DMEM, 11965092, Gibco,

(16) Therapeutic potential of dihydronicotinamide riboside (NRH) on obesity and glucose intolerance in miceNature CommunicationsMarch 25, 2026Marie Rumpler, Guido van Mierlo, Kasper T. Vinten, Maria Pilar Giner, Stefan Christen, Faisal Hayat, Mikhail V. Makarov, Vincent Gardeux, Julie Russeil, Bauke V. Schomakers, et al.were obtained from Gibco (Thermo Fisher Scientific Inc. (Waltham, MA, USA)). AML12 cells (ATCC Cat #: CRL-2254) were cultured in Dulbecco’s modified Eagle’s medium/Nutrient mixture F-12 (DMEM:F-12), supplemented

(17) A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injuryCell Rep MedMarch 17, 2026Zhaoyi Che, Zhihui Luo, Dong Xiao, Fashu Ma, Haoxiong Zhou, Yali Song, Shanshan Guo, Yuan Yuan, Hao Wang, Ching-Pong Mak, et al.Cell lines,Experimental models: Cell lines ,, RAW264.7,ATCC,ATCC Cat#TIB-71 AML12,ATCC,ATCC Cat#CRL-2254 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models:

(18) Novel nucleoside analogs exhibit potent intracellular and in vivo activities against Mycobacterium aviumMicrobiology SpectrumMarch 3, 2026Ho-Sung Park, Do Young Kim, Ji Seok Oh, Dong Hyon Koo, Suyeon Yeom, Seungwoo Kim, Arjun Gontala, Sang-Yeop Lee, Dong Ho Kim, Kyungho Woo, et al.data are provided in the Supplemental material (“Synthetic Procedures”). Cell culture AML12 cells (CRL-2254; ATCC, Manassas, VA, USA), a murine hepatocyte cell line, were cultured in Dulbecco’s modified Eagle’s

(19) Hepatocyte TonEBP promotes metabolic stress-induced hepatic fibroinflammation involving transcriptional activation of ELR⁺ CXC chemokinesCell Death DiscoveryFebruary 26, 2026Jun Ho Lee, Hana Song, Eun Jin Yoo, Yeseul Jeong, Seung Mi Ko, Go Woon Shin, Gee Euhn Choi, Youngheun Jee, Minhyeok Kang, Jiwon Yang, et al.Manassas, VA) were maintained in Eagle’s MEM supplemented with 10% fetal bovine serum (FBS). AML12 cells (CRL-2254; ATCC) were cultured in DMEM containing 10% FBS, insulin (10 μg/mL), transferrin (5.5 μg/mL), selenium

(20) Comprehensive Cytogenetic and Genomic Profiling of the Murine AML12 (Alpha Mouse Liver 12) Hepatocyte Cell LineCellsFebruary 24, 2026Darine Y. Asar, Stefanie Kankel, Diandra T. Keller, Katharina S. Hardt, Sarah K. Schröder-Lange, Eva M. Buhl, Thomas Liehr, Ralf Weiskirchensearches. 2.2. Cell Culture AML12 cells were purchased from the American Type Culture Collection (CRL-2254, ATCC, Manassas, VA, USA). The cells were grown in a humidified incubator at a temperature of 37 °C

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AML12 from ATCC

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