CRFK from ATCC

Product Specs
ItemCRFK
CompanyATCC
Price $318.00Supplier Page View Company Product Page
Catalog NumberCCL-94
SpeciesFelis catus
TypeCell Biology
Validated Species ReactivityCat (Ct)
Age12 weeks
DiseaseNormal
TissueCortex, kidney
ApplicationsThis cell line is a suitable transfection host

Specifications/Features

Post freeze viability ≥ 50%

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Development and Immunogenic Evaluation of a Recombinant Vesicular Stomatitis Virus Expressing Nipah Virus F and G GlycoproteinsVirusesJuly 31, 2025Guo H, Liu R, Pan D, Dang Y, Meng S, Shan D, Wang X, Ge J, Bu Z, Wen Z.catalogue number CCL-185), Crandell–Rees feline kidney cells (CRFK, sourced from ATCC, catalogue number CCL-94), equine embryo kidney cells (Eqek, provided by Professor Du Cheng from the Chinese Academy of Agricultural

(2) Indels allow antiviral proteins to evolve functional novelty inaccessible by missense mutationsCell GenomicsJune 11, 2025Jeannette L. Tenthorey, Serena del Banco, Ishrak Ramzan, Hayley Klingenberg, Chang Liu, Michael Emerman, Harmit S. Malikfemale fetal cell line that is highly transfectable for high-titer virus production; and CRFK (Cat #CCL-94; RRID:CVCL_2426), a female cat fibroblast cell line used for stable expression of TRIM5α variants followed

(3) In Vitro Differential Virucidal Efficacy of Alcohol-Based Disinfectants Against Human Norovirus and Its SurrogatesMicroorganismsFebruary 8, 2025Eri Hiraishi, Keita Ozaki, Moe Yamakami, Tempei Akasaka, Hirokazu Kimuraand 100 mg/L streptomycin (Fujifilm Wako, Osaka, Japan). Crandell–Rees feline kidney (CRFK) cells (CCL-94, ATCC) were cultured at 37 °C under a 5% CO2 atmosphere in DMEM containing 10% heat-inactivated FBS

(4) Early Transcriptional Changes in Feline Herpesvirus-1-Infected Crandell-Rees Feline Kidney CellsVeterinary SciencesOctober 30, 2024Xiuqing Xiao, Fuqiang Xu, Fan Jiainto FHV-1 and host interactions. 2. Materials and Methods 2.1. Cells and Viruses The CRFK cells (CCL-94, ATCC, Manassas, VA, USA) were cultured in DMEM (Gibco, Thermo Fisher, Waltham, MA, USA) supplemented

(5) Antiviral Activity of Nitazoxanide and Miltefosine Against FeHV‐1 In VitroVeterinary Medicine InternationalOctober 19, 2024Consiglia Longobardi, Gianmarco Ferrara, Sara Damiano, Salvatore Florio, Giuseppe Iovane, Ugo Pagnini, Serena Montagnaro, Roberto Ciarcia(Crandell-Rees Feline Kidney Cells, CRFK) cultured in 25 cm2 flasks were used. Briefly, CRFK (CRFK CCL-94, ATCC) were maintained in Dulbecco's modified Eagle's medium (DMEM; Corning) supplemented with 10% fetal

(6) Indels allow antiviral proteins to evolve functional novelty inaccessible by missense mutationsbioRxiv : the preprint server for biologyMay 10, 2024Tenthorey JL, Del Banco S, Ramzan I, Klingenberg H, Liu C, Emerman M, Malik HS.purchased from the ATCC: HEK293T/17 (CRL-11268), a transfectable cell line for virus production, and CRFK (CCL-94), a cat fibroblast cell line. Cell supernatants were tested for mycoplasma contamination by MycoProbe

(7) Guinea pig herpes like virus is a gamma herpesvirusVirus GenesFebruary 10, 2024Brent A. Stanfield, Emmanuelle Ruiz, Vladimir N. Chouljenko, Konstantin G. Kousoulas(CRFK), guinea pig 104c1 GPC-16, and JH4 clone 1 were purchased from ATCC (ATCC, Catalogue #s: CCL-81, CCL-94, CRL-1405, CCL-242, and CCL-158, respectively) and maintained in either DMEM (Gibco, Catalogue #: 11965),

(8) Detection of Felis catus Gammaherpesvirus 1 in Domestic Cat Saliva: Prevalence, Risk Factors, and Attempted Virus IsolationPathogensJanuary 26, 2024Malcolm A. M. Hill, Tracy Satchell, Ryan M. Troyerfrom the American Type Culture Collection (ATCC) included Crandell-Rees feline kidney (CRFK) cells (CCL-94), the feline embryonal tongue cell line Fc3Tg (CCL-176), African green monkey Vero cells (CCL-81), and

(9) Broad-Spectrum, Potent, and Durable Ceria Nanoparticles Inactivate RNA Virus Infectivity by Targeting Virion Surfaces and Disrupting Virus–Receptor InteractionsMoleculesJuly 4, 2023Candace R. Fox, Kritika Kedarinath, Craig J. Neal, Jeremy Sheiber, Elayaraja Kolanthai, Udit Kumar, Christina Drake, Sudipta Seal, Griffith D. ParksFeline Calicivirus (FCV, ATCC, catalog number VR-782) was grown in CRFK cells (ATCC, catalog number CCL-94) at 37 °C. At 1 DPI, media and dislodged cells were harvested and processed by three rounds of quick

(10) The NSs protein of severe fever with thrombocytopenia syndrome virus differentially inhibits the type 1 interferon response among animal speciesThe Journal of biological chemistryJune 1, 2023Yoshikawa R, Kawakami M, Yasuda J.American Type Culture Collection [ATCC]), NIH3T3 (mouse) (CRL-1658; ATCC), MDTF (mouse), CRFK (cat) (CCL-94; ATCC), FEA (cat) (feline embryonic fibroblasts), Cf2Th (dog) (CRL-1430; ATCC), A72 (dog) (CRL-1542;

(11) Feline Calicivirus P39 Inhibits Innate Immune Responses by Autophagic Degradation of Retinoic Acid Inducible Gene IInternational Journal of Molecular SciencesMarch 9, 2023Jianwei Mao, Shaotang Ye, Jie Deng, Jie Song, Zhen Wang, Aolei Chen, Pei Zhou, Shoujun Liembryonic kidney (HEK) 293T cell line (CRL-3216) and Crandell Reese feline kidney (CRFK) cell line (CCL-94) was purchased from ATCC in 2014; it was appropriately cryopreserved, cultured, and passaged in our

(12) Natural Flavonoid Derivatives Have Pan-Coronavirus Antiviral ActivityMicroorganismsJanuary 25, 2023Mattia Mori, Deborah Quaglio, Andrea Calcaterra, Francesca Ghirga, Leonardo Sorrentino, Silvia Cammarone, Matteo Fracella, Alessandra D’Auria, Federica Frasca, Elena Criscuolo, et al.Feline-CoV, Bovine-CoV, and HCoV-OC43 In Vitro Assays and Titration HRT-18 (CCL-244; ATCC) and CRFK (CCL-94; ATCC) cells were cultured in DMEM supplemented with NEAA, P/S, Hepes buffer, and 10% FBS. A clinical

(13) Potent Inactivation of Human Respiratory Viruses Including SARS-CoV-2 by a Photoactivated Self-Cleaning Regenerative Antiviral CoatingACS Applied Materials & InterfacesSeptember 14, 2022Udit Kumar, Candace R. Fox, Elayaraja Kolanthai, Craig J. Neal, Kritika Kedarinath, Yifei Fu, Erik Marcelo, Balaashwin Babu, Griffith D. Parks, Sudipta Sealin 96-well plates. FCV (ATCC, catalog number VR-782) was grown in CRFK cells (ATCC, catalog number CCL-94) at 37 °C. At d1pi, media and dislodged cells were harvested and virus stocks were prepared as described

(14) Development of Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detecting Feline CoronavirusAnimals (Basel)August 14, 2022Witsanu Rapichai, Wichayet Saejung, Kotchaporn Khumtong, Chaiwat Boonkaewwan, Supansa Tuanthap, Peter A. Lieberzeit, Kiattawee Choowongkomon, Jatuporn RattanasrisompornMethods 2.1. Cell and Virus Propagation Samples of the Crandell-Rees Feline Kidney (CRFK) cell line (CCL-94, ATCC) were cultured in Eagle’s Minimum Essential Medium (EMEM) (ATCC 30-2003, USA) supplemented with

(15) Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoproteinCellJune 23, 2022M. Alejandra Tortorici et al.obtained from ATCC: Human cells epithelial embryo (HEK293T, CRL-3216), Felis catus kidney cells (CRFK, CCL-94), Canis familiaris epithelial kidney cells (MDCK, CCL-34), Canis familiaris tumor fibroblast cells (A-72,

(16) Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technologyScientific ReportsApril 27, 2022Sayumi Shimode, Tetsushi Sakuma, Takashi Yamamoto, Takayuki Miyazawafor vaccine production, but also for studies of the function of ERVs. Methods Cell cultures CRFK (CCL-94, ATCC, Manassas, VA, USA), AH927 (feline embryonic fibroblasts)36, and a feline sarcoma-positive leukemia-negative

(17) Surface Modification of E. coli Outer Membrane Vesicles with Glycosylphosphatidylinositol-Anchored Proteins: Generating Pro/Eukaryote Chimera ConstructsMembranesJune 4, 2021Zaruba M, Roschitz L, Sami H, Ogris M, Gerner W, Metzner C.transfected by calcium phosphate co-precipitation into a variety of host cell lines—CrFK (ATCC acc. no. CCL-94), HeLa (ATCC acc. no. CCL-2), HEK293T (ATCC acc. no. CRL-3216), CHO (ATCC acc. no. CCL-61)—and selected

(18) Safety and Efficacy of Felid Herpesvirus-1 Deletion Mutants in CatsVirusesJanuary 22, 2021Yao Lee, Roger K. Maes, John M. Kruger, Matti Kiupel, Kim S. Giessler, Gisela Soboll Husseytwo-step Red-mediated recombination [17]. A and lowtitration, passage virulent clinical the two-step challenge on(CRFK) day 63 a dose of CCL-94) 4 × 105[17]. TCID perSC cat via intranasal instillation USA] and Red-mediated recombination A50 For low passage virulent clinical linevirus kidney cellatline (ATCC, was used. administrations of

(19) CRFK and Primary Macrophages Transcriptomes in Response to Feline Coronavirus Infection Differ SignificantlyFrontiers in GeneticsDecember 3, 2020Yvonne Drechsler, Elton J. R. Vasconcelos, Lisa M. Griggs, Pedro P. P. V. Dinizpathogenesis. Materials and Methods Cell Culture CRFK cells (ATCC, Manassas, VA, United States, cat#: CCL-94) were grown as monolayers in Dulbecco’s Modified Essential Medium (DMEM) containing 10% Fetal Bovine Serum (FBS

(20) High-throughput viral microneutralization method for feline coronavirus using image cytometryJournal of Virological MethodsDecember 1, 2020Morgan Pearson, Alora LaVoy, Leo Li-Ying Chan, Gregg A. Deandrug discovery and vaccine development. 2 Materials and methods 2.1 Host cell preparation CRFK (CCL-94, ATCC, Manassas, VA), an adherent epithelial cell line derived from feline kidney cortex, were cultured

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CRFK from ATCC

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